ACS Nano
·
2025
·
IF 16
Ziang Li,Jincheng Tang,Liang Zhou,Jiannan Mao,Wei Wang,Ziyan Huang,Lichen Zhang,Jie Wu,Xinzhao Jiang,Zhouye Ding,Kun Xi,Feng Cai,Yong Gu,Liang Chen
The extracellular matrix (ECM) stores signaling molecules and facilitates mechanical and biochemical signaling in cells. However, the influence of biomimetic “rejuvenation” ECM structures on aging- and degeneration-related cellular activities and tissue repair is not well understood. We combined physical extrusion and precise “on–off” alternating cross-linking methods to create anisotropic biomaterial microgels (MicroRod and MicroSphere) and explored how they regulate the cell activities of the nucleus pulposus (NP) and their potential antidegenerative effects on intervertebral discs. NP cells exhibited aligned growth along the surface of the MicroRod, enhanced proliferation, and reduced apoptosis. This suggests an adaptive cellular response involving adhesion and mechanosensing, which causes cytoskeletal extension via environmental cues. NP cells maintain nuclear membrane integrity through the YAP/TAZ pathway, which activates the cGAS-STING pathway to rectify the aging mechanisms. In vivo, MicroRod carries NP cells and reduces inflammatory factor and protease secretion in degenerated intervertebral discs, inhibiting degeneration and promoting NP tissue regeneration. Our findings highlight the role of mechanical stress in maintaining cellular activity and antiaging effects in harsh environments, providing a foundation for further research and development of antidegenerative biomaterials.
研究领域:
生物材料
·
组织工程
·
力学生物学
·
细胞衰老
·
退行性疾病
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DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Yuhua Zhu,Song Chen,Cunfang Zhang,Toshiyuki Ikoma,Hongmei Guo,Xinyan Zhang,Xiaona Li,Weiyi Chen
In this study, three types of porous gelatin/HAp composite-based scaffolds were prepared and their microstructure, mechanical property, and in vitro biocompatibility were evaluated. FD-gelatin/HAp scaffolds were prepared via a conventional freeze-drying (FD) method, while DCPD-bound gelatin/HAp (DCPD-gelatin/HAp) microsphere scaffolds and HAp-bound gelatin/HAp (HAp-gelatin/HAp) microsphere scaffolds were prepared via the combined method of emulsion technique and novel microsphere-packing method. It was found that the microstructure and mechanical property of porous gelatin/HAp scaffolds were highly dependent on the preparation method and type of scaffolds. FD-gelatin/HAp scaffolds had few pores with a comparatively flat surface and had a compressive strength was 24 ± 1 MPa. DCPD-gelatin/HAp microsphere scaffolds had a porous structure with the average pore size of 121 ± 12 μm and had the compressive strength of 50 ± 3 MPa. HAp-gelatin/HAp microsphere scaffolds also had a porous structure with the average pore size of 190 ± 26 μm and had the compressive strength of 17 ± 4 MPa. In vitro biocompatibility was performed by culturing the osteoblast-like MC3T3-E1 cells with all types of scaffolds. The results showed that all types of scaffolds were biocompatible to support cell attachment and proliferation. However, a significant difference in cell behavior was clearly observed. Most cells were grown on the surface of FD-gelatin/HAp scaffolds due to the lack of the porous structure, while those were grown on the surface and inside the pore of both DCPD-gelatin/HAp microsphere scaffolds and HAp-gelatin/HAp microsphere scaffolds due to the presence of the porous structure. Thus, the porous gelatin/HAp microsphere-based scaffolds have a potential as novel bone tissue regenerative materials.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
FERROELECTRICS
·
2018
·
IF 0.728
Rui Yang,Qinghua He,Chuhui Wang,Shuqing Sun
We report the surface modification of polystyrene microsphere by ozone treatment. The effects of the ozone treatment on the surface properties of polystyrene microsphere are probed using a series of measurements, including Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy(XPS), scanning electron microscope(SEM), fluorescence microscope. As a result of above measurements, the surface of the sample contains a large number of carboxyl and hydroxy (mainly –COOH) groups. In addition, the polystyrene microsphere successfully connected with amino quantum dots which demonstrates the existence of functional groups. A major conclusion that can be drawn from the results and analysis described in this work is that the ozone treatment provides a feasible method for surface modification of polystyrene microsphere in the use of biological testing. The method is simple and universal, and the operation is simple and convenient to modify the carboxyl group.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Yong Pan,Xiangyang Luan,Yue Jing,Yu Jiang,Li Ding
Hydrogenation reaction is a highly dangerous yet widely applied chemical process. Monitoring hydrogen leakage during this reaction is essential to ensure industrial safety. To address this need, a highly sensitive hydrogen sensor has been developed based on Pt sensitized ZnO microsphere as sensing materials, which was prepared by annealing-assisted one-pot solvothermal method. Compared with the previously reported work, this strategy enables the controlled synthesis of uniform and monodisperse ZnO microspheres, ensuring highly controllable and reproducible precious metal loading, which is conducive to large-scale production. Thanks to higher oxygen vacancy concentrations, enhanced mesopore volume fractions, and a larger specific surface area, the 1 at% Pt-ZnO sensor at an annealing temperature of 500 °C demonstrates excellent hydrogen detection performance, including a higher response value (1,480,000), a lower detection limit (100 ppb), outstanding selectivity (response to interfering gases <1%), and good short-term repeatability (relative standard deviation of 6.98% over 8 cycles). Furthermore, the sensor has also been applied in the hydrogenation process of 2,4-dinitrotoluene for hydrogen leak monitoring, effectively detecting hydrogen leaks at concentrations of 30-40 ppm, confirming its potential for leak detection in typical hydrogenation reaction.
✓ 已验证
DOI
高影响因子
OPTICAL MATERIALS
·
2022
·
IF 3.754
Huimin Yu,Xueqiong Su,Yong Pan,Dongwen Gao,Jin Wang,Ruixiang Chen,Junhe Zhang,Fei Dou,Xinping Zhang,Kun Ge,Xiaoyu Shi,Tianrui Zhai,Li Wang
The whispering gallery mode (WGM) microcavity has a high-quality factor and small mode volume, for the property of confining photons in a small volume for a long time, which enhances the light-matter interaction. Combining with quantum dots that act as gain medium, it can realize low-threshold and narrow-linewidth microlasers with excellent performance. However, achieving conventional whispering gallery mode lasers still requires complex preparation conditions. Here, we report a narrow linewidth whispering gallery mode lasing from all-inorganic perovskite quantum dots (CsPbBr 3 QDs), illustrating facile and cost-effective properties. CsPbBr 3 QDs with excellent properties were synthesized by hot injection method and used as gain media for lasers, and microspheres as optical resonators . The whispering gallery mode laser with a quality factor Q of 4791 and a narrow linewidth of 0.11 nm was obtained at room temperature. The WGM microlaser achieved by this paper could be a great application prospects in biotechnology.
研究领域:
光学
·
光子学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Xiaoyou Wang,Lei Wang,Simin Peng,Xin Luo,Zhenzhen Zhang,Tao Wu,Jungang Jiang,Yifan Zhang
The desire for healthy living has created a crucial need for portable flexible health-monitoring devices based on biomaterials. Toward this end, we report a microsphere-structured hydrogel that uses bovine serum albumin (BSA) as a dielectric layer for capacitive pressure sensors. We developed a theoretical model that describes how stacking dielectric layers of spheres affects the performance of capacitive sensors. We also prepared a prototype sensor featuring the unique microsphere structure to create capacitive sensors with high sensitivity (360.91 strain sensitivity), excellent cyclical stability, and a long service life (over 5000 stretching–compression cycles). Furthermore, the design of the hydrogel sensor allows for easy integration into fabrics to create devices such as smart wristbands, which can collect a diverse range of health data. Thus, BSA-hydrogel-based sensors not only provide safe wearable devices but also advance the performance of high-sensitivity capacitive sensors.
研究领域:
生物材料学
·
传感器技术
·
医学工程
✓ 已验证
DOI
高影响因子
Journal of Biomaterials and Tissue Engineering
·
2021
·Vol. 11
·期 1
·1-13
·
IF 0.287
Le Guo-Ping,Xi Li-Cheng,Zhai Jin-Min
Objective: To fabricate and characterize sustained release antibacterial microsphere composite scaffolds for bone tissue engineering. Methods: First, poly(lactic acid glycolic acid) (PLGA) copolymer, chitosan (CS), and hyaluronic acid (HA) were used to prepare porous microspheres
by the emulsion method. Vancomycin (VA) hydrochloride was loaded onto the microspheres (VA@PLGA-CS-HA) for sustained release of drug, after which hydroxyapatite (HAP) was added (VA@PLGA-CS-HA/HAP). The detailed in vitro characterization of the antibacterial sustained-release microspheres
was performed using various techniques including the Cell Counting Kit-8 assay; hemolysis, osteogenic, and antibacterial experiments; scanning electron microscopy (SEM); and mechanical testing. Results: SEM showed that the sustained-release PLGA-CS-HA microspheres had a regular spherical
shape, and the composite scaffolds of VA@PLGA-CS-HA/HAP microspheres were compact in structure. The relative colony-forming unit was less than 3% after 14 d of intervention. Cytotoxicity testing revealed that the relative growth rate of MC3T3-E1 and MG-63 osteoblast cells was more than 80%
when the concentration of VA did not exceed 50 mg/mL. The mechanical strength of the scaffolds was comparable to that of human cancellous bone. The composite scaffolds enhanced the alkaline phosphatase activity of MG-63 cells and promoted the mRNA expression of osteogenic genes such as runt-related
transcription factor 2, osteocalcin, osteopontin, and collagen. Conclusion: VA@PLGA-CS-HA microspheres can control the slow and sustained release of VA in vitro. The VA@PLGA-CS-HA/HAP microsphere composite scaffolds has a slower degradation rate and good antibacterial and mechanical
properties in vitro.
研究领域:
药学
·
材料科学
·
生物医学工程
·
组织工程
✓ 已验证
DOI
高影响因子
POLYMER
·
2020
·
IF 4.231
Chunhua Wang,Lan Yang,Yujing He,Huining Xiao,Wei Lin
In this work, the vinyl groups were introduced onto gelatin to create methacrylated gelatin nanoparticles (MA-GNP) as crosslinking centers for fabricating macromolecular microsphere composite (MMC) hydrogels. The MA-GNPs with an average size of ~100 nm, measured with dynamic light scattering , were also visualized using AFM . The presence of C C bonds in the nanoparticles was further confirmed with the proton NMR . Then, the MA-GNP was incorporated as a cross-linker to synthesize MMC hydrogels using acrylamide as the monomer . The resulting hydrogels are porous with tunable pore sizes; and exhibit the improved compressive resistance and elasticity. Increasing the dosage of MA-GNP appropriately led to the reduction of the equilibrium swelling ratio but improved thermal stability of the gels. Moreover, the MA-GNP can endow the gels with enhanced biocompatibility. Overall, the novel protein-based nanospheres are of great potential as both nano-crosslinker and reinforcer to create polymer hydrogels with tailored properties.
研究领域:
材料科学
·
生物医学工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
·
2016
·
IF 6.008
Jianbing Wu,Zhaozhu Zheng,Gang Li,David L. Kaplan,Xiaoqin Wang
A one step, rapid method to prepare silk microspheres was developed, with particle size controlled by the addition of polyethylene glycol (PEG). PEG molecular weight (4.0 K–20.0 KDa) and concentration (20–50 wt%), as well as silk concentration (5–20 wt%), were key factors that determined particle sizes varying in a range of 1–100 μm. Addition of methanol to the PEG-silk combinations increased the content of crystalline β-sheet in the silk microspheres. To track the distribution and degradation of silk microspheres in vivo , 3-mercaptopropionic acid (MPA)-coated CdTe quantum dots (QDs) were physically entrapped in the silk microspheres. QDs tightly bound to the β-sheet domains of silk via hydrophobic interactions, with over 96% of the loaded QDs remaining in the silk microspheres after exhaustive extraction. The fluorescence of QDs-incorporated silk microspheres less stable in cell culture medium than in phosphate buffer solution (PBS) and water. After subcutaneous injection in mice, microspheres prepared from 20% silk (approx. 30 μm diameter particles) still fluoresced at 24 h, while those prepared from 8% silk (approx. 4 μm diameter particles) and free QDs were not detectable, reflecting the QDs quenching and particle size effect on microsphere clearance in vivo . The larger microspheres were more resistant to cell internalization and degradation. Since PEG is an FDA-approved polymer, and silk is FDA approved for some medical devices , the methods developed in the present study will be useful in a variety of biomedical applications where simple, rapid and scalable preparation of silk microspheres is required. Statement of Significance The work is of significance to the biomaterial and controlled release society because it provides a new option for fabricating silk microspheres in one simple step of mixing silk and polyethylene glycol (PEG), with the size and properties of microspheres controllable by PEG molecular weight as well as PEG and silk concentrations. Although fabrication of silk microspheres have been reported previously using spray-drying, liposome-templating, polyvinyl alcohol (PVA) emulsification , etc., applications were hindered due to harsh conditions (temperature, solvents, etc.) and complicated procedures used as well as low yield and less controllable particle size (usually <10 μm). Since PEG is an FDA-approved polymer, and silk is FDA approved for some medical devices, the methods developed in the present study will be useful in a variety of biomedical applications where simple, rapid and scalable preparation of silk microspheres is required.
研究领域:
生物材料学
·
控释系统
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micro & Nano Letters
·
2017
·Vol. 12
·期 6
·386-390
·
IF 0.723
Lv Ming-zhe,Wang Li-feng,Fang Lei,Li Pu-wang,Li Si-dong
Chitosan microspheres were prepared by ion induction and chemical cross-linking using water as the medium. Natural rubber/chitosan microsphere blends were then prepared by blending the chitosan microsphere suspension with natural rubber latex. An infrared spectrometer, particle size analyser, scanning electron microscope, surface contact angle analyser, thermogravimetric analyser, and differential scanning calorimeter were employed for structure and property characterisation. The results showed that the average particle diameter and the polydispersity index of chitosan microspheres were 323.8 nm and 0.283, respectively. The thermal stability and mechanical properties of natural rubber/chitosan blends were enhanced by introducing chitosan microspheres into natural rubber latex. The water contact angle was significantly decreased, from 94.64° to 55.47°, indicating improved hydrophilic properties for the natural rubber/chitosan film. The natural rubber/chitosan blends showed excellent antibacterial properties when the chitosan microsphere content was >4.00% w/w.
研究领域:
材料科学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2024
·
IF 4.1
Chen Chang,Xuyang Wang,Xingyan Zeng,Yingfeng Wen,Hui Nie,Li Chang,Xingping Zhou,Xiaolin Xie,Lin Ye,Yiu-Wing Mai
The development of lithium-sulfur (Li–S) batteries is hindered by inhomogeneous Li plating and irreversible loss of active materials. In this work, a thermal conductive and electrocatalytic aramid nanofiber (ANF) composite separator containing surface-decorated alumina (Al 2 O 3 ) microspheres is prepared through a simple filtration approach using ZnO nanosheets decorated Al 2 O 3 microspheres (Al 2 O 3 @ZnO) as the thermal conductive filler and electrocatalyst. Compared with the separators fabricated by surface modification of commercial polyolefin separators, the as-prepared ANF/Al 2 O 3 @ZnO composite separator features homogeneous compositions and ensures uniform thermal distribution in both the through-plane and in-plane directions of the membrane. By rationally designing the structure of Al 2 O 3 @ZnO and adjusting the mass ratio of Al 2 O 3 @ZnO to ANF, excellent thermal conduction networks are formed by overlapping the ZnO nanosheets. Hence, the prepared ANF/Al 2 O 3 @ZnO composite separator possesses higher thermal conductivity than ANF/Al 2 O 3 composite separator, which benefits uniform thermal distribution and homogeneous Li plating within the battery. Moreover, the electrocatalytic ZnO nanosheets catalyze the conversion of lithium polysulfides, efficiently inhibiting the shuttle effect and improving sulfur utilization. When subjected to a temperature gradient, the ANF/Al 2 O 3 @ZnO composite separator assembled cells display high initial capacity, low decay rate, and superb cycle stability. This novel composite separator integrates both thermal conductive and electrocatalytic functional components with the heat-resistant ANF matrix, offering a new design strategy of separator towards high-performance Li–S batteries.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Green Chemical Engineering
·
2020
·
IF 0
Xing Jiang,Jian Ren,Yong Kong,Zhiyang Zhao,Xiaodong Shen,Maohong Fan
The purpose of this study was to prepare a proof-of-concept CO 2 adsorbing material based on a new amine grafted silica aerogel microsphere (AGSAM) that could be used on both fixed and fluidized beds. A low-cost water glass and environment-friendly water were used as precursor and solvent of the silica aerogel, respectively. The silica aerogel microsphere (SAM) was prepared by dropping the siliceous solution into hot oil bath. The effect of the pH value of the siliceous solution, stirring speed and stirring paddle position on the sphericity and size of the SAM was investigated. The SAM with good sphericity was obtained when the pH value was 5.69–5.79. The mean diameter of the SAM decreased from 5 to 1 mm when the stirring speed increased from 1000 to 2000 rpm. The SAM with excellent sphericity was prepared when the paddle was placed on the top of the oil bath. When the paddle was placed at the middle and bottom of the oil bath, some liquid drops aggregated together and formed large aggregations. AGSAM was obtained by grafting the amine groups onto the framework of the silica gel microsphere. The CO 2 adsorption capacity of the AGSAM was 1.04 mmol g -1 with 1% CO 2 at 300 mL min -1 . The AGSAM with 4, 3.2, 2 and 1 mm in diameters had the fluidizing velocities of 0.531, 0.425, 0.265 and 0.159 m s -1 , respectively. The AGSAM with different sizes met different fluidizing conditions.
研究领域:
材料科学
·
环境科学
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DOI
危险品化学品经营许可证（带存储）