MICROCHIMICA ACTA
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2023
·Vol. 191
·期 1
·1-11
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IF 5.7
Lu Yuyang,Shen Nuotong,Xi Yachao,Zhu Tao,Peng Hao,Zhong Lihao,Li Fang
Mn 3 O 4 nanozyme with good oxidase-like activity was successfully synthesized. The prepared Mn 3 O 4 nanozyme can directly and effectively catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate green–blue-colored ox-TMB. Creatinine exhibits distinct inhibition effect on Mn 3 O 4 nanozyme-catalyzed TMB colorimetric reaction system, leading to obvious color fading and absorbance intensity decrease of the reaction system. Furthermore, interference from uric acid can be effectively eliminated by regulating the pH of TMB-Mn 3 O 4 colorimetric reaction system to pH 2.0. Then, a simple and bioenzyme-free colorimetric assay for the determination of creatinine was developed based on TMB-Mn 3 O 4 colorimetric reaction. The linear detection range is from 100 to 800 μM and from 1 to 20 mM. The lowest limit of detection is 35.3 μM. Satisfied results are obtained for the determination of creatinine in real urine and sweat samples. This work provides the synthesis of a good oxidase-like nanozyme Mn 3 O 4 and presents the fabrication of an effective nanozyme-based bioenzyme-free colorimetric assay for the determination of creatinine. Graphical Abstract
研究领域:
生物化学
·
医学诊断
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xuechao Xu,Juanli Yang,Guijie Hao,Bo Wang,Tong Ma,Suping Zhu,Lu Gao,Zhen-quan Yang
A multifunctional oxidase-mimicking Ag/Mn 3 O 4 was prepared, catalyzing the 3, 3′, 5, 5′-tetramethylbenzidine (TMB) chromogenic reaction. Six foodborne pathogenic bacteria species, including Escherichia coli , Staphylococcus aureus , Salmonella enterica , Listeria monocytogenes , Bacillus cereus , and Cronobacter sakazakii , were observed to differentially inhibit its oxidase-like activity, resulting in decelerating the TMB chromogenic reaction. Owing to these properties, the following achievements were achieved: colorimetric determination of these bacteria with high sensitivity can be achieved using Ag/Mn 3 O 4 + TMB reaction system; precise identification of these bacteria at different concentrations, including individual bacterium, binary mixtures, and even multivariate mixtures, can be effectively realized by combining the Ag/Mn 3 O 4 -based colorimetric sensor array with principal component analysis (PCA); broad-spectrum inactivation of these bacteria can be remarkably realized through catalyzation of Ag/Mn 3 O 4 to generate superoxide anion free radicals. Therefore, our proposed Ag/Mn 3 O 4 holds significant application potential in the colorimetric determination, precise identification, and broad-spectrum inactivation of foodborne pathogenic bacteria.
研究领域:
食品安全
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生物传感
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微生物学
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DOI
PubMed
高影响因子
Nanoscale
·
2025
·
IF 5.1
Minghui Li,Song Wang,Lingling Chang,Ruiting Chen,Yuhang Liu,Zhengjie Ye,Yuhang Zhao,YiFan Ma,Jing Yang,Xinyu Gan,Yongzhi Zhuang,Peng Wang
Background: Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder of uncertain etiology. Current studies suggest that ASD progression is closely linked to an imbalance between oxidative stress and antioxidant capacity, marked by elevated levels of reactive oxygen species (ROS) and reduced concentrations of antioxidant molecules such as superoxide dismutase (SOD) and glutathione (GSH). Although the human body does possess endogenous ROS-scavenging enzymes, their sensitivity to environmental conditions and the difficulties of large-scale production limit their practical application. Consequently, substantial efforts have been dedicated in recent years to developing artificial enzymes with ROS-scavenging activity. Among these, ROS-scavenging nanozymes have been widely used due to their enhanced stability and multifunctionality. Notably, only a few manganese-containing nanozymes have been reported to exhibit effective reactive oxygen species (ROS) scavenging activity thus far. Methods: In this study, we utilized Mn3O4 nanozymes (Mn3O4 NZs) exhibiting superoxide dismutase, catalase, and hydroxyl radical–scavenging activities. We assessed brain injury, as well as the antioxidative and anti-inflammatory effects of Mn3O4 NZs through behavioral tests, Nissl staining, immunofluorescence assays, and a laser speckle imaging system. Furthermore, we explored the underlying mechanisms of Mn3O4 NZs by employing ELISA kits, oxidative stress detection kits, and immunofluorescence analysis. Results: The results demonstrated that Mn3O4 NZs increase cerebral blood flow and effectively ameliorate ischemic and hypoxic conditions in BTBR mice. Moreover, they improve social deficits, repetitive stereotyped behaviors, cognitive impairment, and neuronal morphological damage. Further in vitro experiments confirmed that Mn3O4 NZs exert neuroprotective effects in BTBR mice by mitigating oxidative stress and inflammation. Conclusion: These findings indicate that Mn3O4 NZs exhibit excellent antioxidant and anti-inflammatory effects in vitro and effectively enhance cerebral blood flow, ameliorate behavioral deficits, and alleviate neuronal damage in BTBR mice in vivo. Collectively, our results suggest that Mn3O4 NZs exert neuroprotective effects in the hippocampus of BTBR mice by reducing oxidative stress, mitigating neuroinflammation, and rescuing neuronal injury. Consequently, they hold promise as a potential nanomaterial for the treatment of autism.
研究领域:
神经科学
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纳米医学
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药理学
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分子生物学
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行为科学
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PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 11
·2484-2497
·
IF 3.641
Li Guiyin,Chen Min,Li Haimei,Li Xinhao,Yao Xiaoqing,Li Liuxun
Golgi protein 73 (GP73) is a novel biomarker for the detection of hepatocellular carcinoma. We prepare a colorimetric aptasensor for GP73 detection based on the catalytic performance of the H-rGO-Mn3O4 nanozyme. The H-rGO-Mn3O4 nanozyme serves
as a peroxidase mimetic which can enhance the reaction with the substrate (3, 3,’ 5, 5′-tetramethylbenzidine, TMB) and H2O2. The peroxidase-like activity of H-rGO-Mn3O4 features a 1.78-fold higher binding affinity value (Km
)
for TMB compared with that of horseradish peroxidase. The H-rGO-Mn3O4 nanozyme not only exhibits admirable peroxidase-like activities due to the synergistic effect of Mn3O4 NPs, rGO and hemin, but also has a large specific surface area to endow the
GP73 aptamer with specific recognition capabilities. A sandwich colorimetric aptasensor is formed to realize the visual detection of GP73 through catalyzing the H2O2-mediated oxidation peroxidase substrate TMB to oxidized TMB accompanied by a color change from colorless
to blue. Under optimal conditions, the logarithm of the GP73 concentration (0.05–50.0 ng/mL) shows a good linear relationship with the absorbance. The calibration equation is Y =−0.0383 lgC+0.4835, with R2 of 0.9964, and the lowest limit of detection is 36.94
pg/mL. Additionally, the content of GP73 in human serum samples is directly detected, and the relative standard deviation is 0.49–4.91%. Compared with the enzyme-linked immunosorbent assay method, the relative error is 0.23–3.61%. All in all, colorimetric aptasensor is demonstrated
to exhibit excellent specificity, stability, and reproducibility.
研究领域:
生物标志物研究
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纳米技术
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分析化学
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癌症诊断
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生物化学
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DOI
高影响因子
Sensors and Actuators Reports
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2025
·
IF 7.6
Junting Liang,Dihan Duan,Lubing Sun,Jinming Li,Mengke Wang,Ziyi Chang,Rick F. Thorne,Chuanliang Chen,Demin Duan
Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn 3 O 4 nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K m value of 0.1896 mM and a high V max of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn 3 O 4 nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.
研究领域:
生物化学
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分析化学
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纳米技术
·
疾病诊断
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenping Yang,Zufei Jin,Ying Yu,Guangyan Hao,Langjin Zhong,Yue Tang,Yuangen Wu
The presence of excessive nitrite in foodstuffs poses significant risks to human health, underscoring the critical importance of developing rapid detection methods. This study synthesizes a Mn₃O₄ vacancy-rich nanozyme exhibiting exclusive oxidase-mimicking activity, capable of catalyzing the oxidation of N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS). Intriguingly, TOPS oxidation derivatives demonstrate nitrite concentration-dependent color variations through a diazotization-like phenomenon. Thus, we establish dual detection platforms incorporating both visual and smartphone-assisted sensing modalities. Mechanistic investigations reveal that both the catalytic oxidation and diazotization-like processes involve reactive oxygen species ( • O₂ − and 1 O₂). Sensitivity studies demonstrate a linear detection range of 20–160 μM, with calculated limits of detection (LOD) at 1.9 μM (visual) and 0.01 μM (smartphone), respectively. Comparative analysis with national standard methods validates the reliability and accuracy of our approach in real food matrices. This work presents a robust analytical platform combining operational simplicity with laboratory-grade precision for food safety monitoring.
研究领域:
分析化学
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纳米技术
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食品安全
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生物传感器
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）