Xing-Yu Long,Jia-Yuan Li,Dong Sheng,Hong-Zhen Lian
The spinel-type magnetic manganese ferrite (MnFe 2 O 4 ) microspheres synthesized by simple solvothermal method were used as a novel adsorbent for selective enrichment and effective isolation of phosphopeptides. The uniform MnFe 2 O 4 magnetic affinity microspheres (MAMSs) had a narrow particle size distribution between 250 and 260 nm, and displayed superparamagnetism with a saturation magnetization value of 67.0 emu/g. Comprehensively, the possible formation mechanism of MnFe 2 O 4 microspheres with ferric and manganous sources as dual precursors was elucidated by comparison with those of Fe 3 O 4 nanoparticles and MnOOH nanosheets respectively with either ferric or manganous source as single precursor. It was suggested that the spherical or sheet nanostructures could be achieved via secondary recrystallization or Ostwald ripening . The MnFe 2 O 4 MAMSs probe exhibited excellent dispersibility in aqueous solution, and rapid magnetic separation within 15 s, as well as good reusability. More importantly, MnFe 2 O 4 was highly selective for phosphopeptides because of the strong coordination interaction between metal ions (Fe 3+ and Mn 2+ ) and phosphate groups of phosphopeptdies. This high specificity was demonstrated by effectively enriching phosphopeptides from digest mixture of β -casein and bovine serum albumin (BSA) with high content of non-phosphopeptides, and embodied further in phosphopeptides enrichment from non-fat milk digests and human serum. Consequently, the prepared MnFe 2 O 4 affinity materials are expected to possess great potential in phosphoproteome research.
In this study, MnFe 2 O 4 microspheres were synthesized to activate potassium persulfate complex salt (Oxone) for the degradation of 17β-estradiol (17β-E2) in aqueous solutions. The characteristic of MnFe 2 O 4 was detected by XRD, XPS and SEM-EDS. The experimental results indicated that the degradation of 17β-E2 followed pseudo-first-order kinetics. At 25 °C, 17β-E2 concentration of 0.5 mg/L, MnFe 2 O 4 dosage of 100 mg/L, Oxone dosage of 0.5 mmol/L, and initial pH value of 6.5, the decomposition efficiency of 17β-E2 reached 82.9% after 30 min of reaction. Additionally, free radical quenching experiments and electron paramagnetic resonance analysis demonstrated that SO 4 − • and •OH participated in the reaction process of the whole reaction system, with SO 4 − • being the main reactive oxygen species (ROS). The activation mechanism of the MnFe 2 O 4 /Oxone/17β-E2 system is proposed as follows: MnFe 2 O 4 initially reacts with O 2 and H 2 O in solution to generate active Fe 3+ -OH and Mn 2+ -OH species. Subsequently, Fe 3+ -OH and Mn 2+ -OH react with Oxone in a heterogeneous phase activation process, producing highly reactive free radicals. After four cycles of MnFe 2 O 4 material, the removal rate of 17β-E2 decreased by 24.1%.
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