MnO2-graphene oxide hybrid nanomaterial with oxidase-like activity for ultrasensitive colorimetric detection of cancer cells
Robust and sensitive cell-based enzyme-linked immunosorbent assay (CELISA) is of great significance in the diagnosis and screening of cancer. However, the method is limited by the high rate of negative results attributed to the instability of horseradish peroxidase (HRP), H 2 O 2 , and antibody. Here, we construct a folic acid–functionalized in situ-grown MnO 2 nanosheet/graphene oxide hybrid (FA-MnO 2 /GO) with oxidase-like activity instead of the anti-folate receptor antibody in traditional CELISA to resist the possible negative interference arising from unstable HRP, H 2 O 2 , and antibodies for more robust colorimetric detection of cancer cells. The functionalization of FA enables the selective binding between hybrid and cancer cells through the over-expressed folate receptor, and then the binding events are converted into quantitative colorimetric signals though the oxidation of the chromogenic substrate TMB catalyzed by MnO 2 , allowing the detection of cancer cells with colorimetric method. Moreover, the construction of MnO 2 /GO hybrid can synergistically enhance the oxidase-like activity of MnO 2 and promote its dispersion in water, further ensuring the accuracy and sensitivity of the detection. A detection limit of 20 cancer cells is obtained by a plate reader, which is lower than those obtained by most reported CELISA methods for cancer cell detection, and as few as 75 cancer cells can be identified by the naked eye. This study not only provides a multifunctional sensing platform for robust and sensitive cancer cell detection, but also offers a promising oxidase-like mimic in the field of bioanalysis. Graphical abstract
危险品化学品经营许可证（带存储）