PHYTOMEDICINE
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2025
·
IF 8.3
Junsong Jing,Yi Sun,Yiyang Shui,Junyi Wang,Wenjing Ye,Ranran Chen,Lianhao Wu,Lijuan Xing,Rongrong Huang,Ting Zhou,Wenwen Zhu,Yueguo Wu,Sheng Zhang,Jing Shi,Yuanyuan Li,Yan Liu,Zhenqiang You
Background Viral pneumonia is an infection of the lungs caused by numerous different viruses, which can lead to severe respiratory distress and even life-threatening conditions. In the absence of specific treatments for viral pneumonia, natural traditional medicines offer an alternative in terms of innovative drug therapies. Morus alba L. (common name mulberry leaf) is a Chinese medicine that has been used clinically as an antiviral. Purpose The therapeutic effect of M. alba on viral pneumonia was investigated along with its mechanism of action. Methods Network pharmacology and molecular docking were used to analyze the mechanism of action of M. alba in the treatment of viral pneumonia. Histology, immunofluorescence, Western blotting, qPCR, and flow cytometry were used to evaluate the protective effect of MLE (the ethanol extract of Morus alba L. ) on PR8 (A/Puerto Rico/8/1934 H1N1, a murine lung-adapted influenza A virus strain)-induced viral pneumonia. SiRNA was used to validate the relationship between the therapeutic effects of MLE on viral pneumonia and the target Syk (a crucial non-receptor tyrosine kinase). Results MLE alleviated PR8-induced viral pneumonia by reducing inflammatory factor expression in the lungs, decreasing NF-κB pathway activation, slowing oxidative damage in the lungs, and inhibiting lung tissue cell apoptosis. Meanwhile, MLE for viral pneumonia was significantly associated with Syk targets. Notably, knockdown of the Syk gene not only reduced the therapeutic effect of MLE, but also suppressed PR8-induced viral pneumonia. Conclusion MLE can alleviate PR8-induced viral pneumonia through inhibiting the Dectin-1/Syk pathway.
研究领域:
药理学
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分子生物学
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病毒学
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2023
·
IF 6
Yan Xu,Tianran Huang,Yongqiang Huang,Ming Huang
Heterocyclic amines (HAs) are carcinogenic compounds produced by the Maillard reaction or pyrolysis of protein-rich foods under thermal processing conditions. The inhibitory effects of mulberry leaf extract on HA formation and flavor changes in braised Muscovy ducks were investigated. The phenolic compounds were found to significantly inhibit HA formation. In particular, tetrahydroxyisoflavone was significantly effective at inhibiting free HAs, with an inhibition rate of 64.43%. The polyphenol mixture (chlorogenic acid, quercetin, kaempferol, luteolin, and tetrahydroxyisoflavone) was effective at inhibiting bound HAs, with an inhibition rate of 43.44%. The addition of quercetin and luteolin significantly reduced the cooking loss of braised Muscovy ducks. Further, phenolic marination had a significant impact on color and water distribution. Using GC-IMS, 45 volatile flavor compounds were identified. Principal component analysis revealed differences in the flavor compounds of the braised Muscovy duck. Collectively, this study provides insights into the mitigation of HA formation for producing safe and healthy meat and meat products.
研究领域:
食品科学
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化学
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营养学
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毒理学
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DOI
高影响因子
Nutrients
·
2023
·Vol. 15
·期 8
·1889
·
IF 5.9
Tae Sik Sung,Seung-Bum Ryoo,Chang-Hyun Lee,Seon-Min Choi,Joo-Won Nam,Hyun-Bok Kim,Ji young Lee,Jung-Dae Lim,Kyu Joo Park,Hyun-Tai Lee
The fruit ofMorus albaL. (MAF) has been consumed as a food worldwide. MAF has also been widely used in traditional medicine for thousands of years in East Asia, and its diverse bioactivities have been reported in numerous publications. However, no prokinetic activity has been reported for MAF or its components. In the present study, therefore, we investigated the effects of MAF on gastrointestinal motor function by measuring the intestinal transit rate (ITR) of Evans blue in mice in vivo. The ITR values accelerated by MAF were significantly higher than those accelerated by cisapride or metoclopramide, suggesting that MAF has potential as a new prokinetic agent to replace cisapride and metoclopramide. We also investigated the effects of MAF on myogenic and neurogenic contractions in human intestinal smooth muscles by measuring spontaneous contractions of smooth muscle strips, smooth muscle contractions induced by neural stimulation, and migrating motor complexes from intestinal segments in the human ileum and sigmoid colon in situ. MAF increased both myogenic and neurogenic contractions to enhance ileal and colonic motility in the human intestine. Taken together, these results indicate that MAF enhanced intestinal motility by increasing both myogenic and neurogenic contractions, thereby accelerating the ITR.Keywords:intestinal transit;Morus alba;mulberry fruit;myogenic contraction;neurogenic contraction;prokinetic;smooth muscle
研究领域:
药理学
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胃肠病学
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DOI
PubMed
高影响因子
Journal of Agriculture and Food Research
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2025
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IF 6.2
Ziyang Wang,Qinqin Ouyang,Xiaolin Liang,Hongxia Li,Haiying Guo,Rong Liu
Traditional Chinese medicine polysaccharides (TCMPs) have attracted growing attention due to their multi-target and multi-level therapeutic effects in the treatment of diabetes. In this study, we systematically evaluated the antidiabetic effect of three TCMPs, including Moringa oleifera Lam. polysaccharide (MOP), Morus alba L. polysaccharide (MLP) and Lycium barbarum polysaccharide (LBP) in T2DM mouse model. Among the three, MLP exhibited the most pronounced therapeutic efficacy, markedly alleviating hyperglycemia, insulin resistance, hepatic steatosis, and pathological lesions in the liver, pancreas. Liver metabolomic profiling revealed that MLP significantly modulated key glucose and lipid metabolic pathways, particularly bile acid and glycerolipid metabolism. Furthermore, we found MLP exerted its antidiabetic action through inhibition of the mTORC1 signaling pathway, leading to suppressed lipogenesis and enhanced fatty acid oxidation. Notably, MLP-mediated mTORC1 inhibition activated the PPARα–SIRT1 axis, thereby downregulating FOXO1-driven gluconeogenesis and promoting GLUT4-mediated glucose uptake. These findings suggest that MLP exerts comprehensive antidiabetic effects by targeting mTORC1-mediated metabolic reprogramming, offering promise as a natural therapeutic agent for T2DM.
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高影响因子
PLoS One
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2013
·Vol. 8
·期 7
·e71144
·
IF 3.73
Yihai Wang,Limin Xiang,Chunhua Wang,Chao Tang,Xiangjiu He
The antidiabetic and antioxidant activities of the ethyl acetate-soluble extract (MFE) of mulberry fruit (Morus alba L.) were investigated. In vitro , MFE showed potent α-glucosidase inhibitory activity and radical-scavenging activities against DPPH and superoxide anion radicals. In vivo , MFE could significantly decrease fasting blood glucose (FBG) and glycosylated serum protein (GSP), and increase antioxidant enzymatic activities (SOD, CAT, GSH-Px) in streptozotocin (STZ)-induced diabetic mice. Bioactivity-guided fractionation of the MFE led to the isolation of 25 phenolic compounds, and their structures were identified on the basis of MS and NMR data. All the 25 compounds were isolated from mulberry fruit for the first time. Also, the α-glucosidase inhibitory activity and antioxidant activity of the phenolics were evaluated. Potent α-glucosidase inhibitory and radical-scavenging activities of these phenolics suggested that they may be partially responsible for the antidiabetic and antioxidant activities of mulberry fruit.
研究领域:
药理学
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生物化学
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营养学
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植物科学
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产品关联
DOI
PubMed
高影响因子
Metabolites
·
2022
·Vol. 12
·期 2
·106
·
IF 5.581
Cui Wu,Huijun Wang,Zhenying Liu,Bo Xu,Zhuojun Li,Pingping Song,Zhimao Chao
The differences in metabolites in male and female individuals of dioeciousMorus albaL. (Moraceae) are usually ignored and lack study. In the present study, 58 leaves and 61 stem barks from male and female individuals were analyzed by untargeted metabolomics via headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) coupled with chemometrics, including principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). A total of 66 and 44 metabolites were identified from leaves and stem barks, respectively. Four and eight differential metabolites among candidate metabolites in leaves and stem barks from male and female individuals were identified. Moreover, females possessed stronger antioxidant activity than males. This is the first report where untargeted metabolomics coupled with chemometrics was used to analyze the different metabolites and to discriminate the gender of leaves and stem barks of dioeciousM. alba. It provided the basis for further study ofM. albaand reference value for researching dioecious plants.Keywords:Morus albaL.;dioecious plant;leaves;stem barks;untargeted metabolomics;chemometrics
研究领域:
植物学
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代谢组学
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药用植物研究
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2015
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IF 4.074
Qingxia Yuan,Yufeng Xie,Wei Wang,Yuhua Yan,Hong Ye,Saqib Jabbar,Xiaoxiong Zeng
Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0 ± 0.5% for MLP were determined as follows: extraction temperature 92 °C, extraction time 3.5 h and ratio (v/w, mL/g) of extraction solvent (water) to raw material 34. Two purified fractions, MLP-3a and MLP-3b with molecular weights of 80.99 and 3.64 kDa, respectively, were obtained from crude MLP by chromatography of DEAE-Cellulose 52 and Sephadex G-100. Fourier transform-infrared spectroscopy revealed that crude MLP, MLP-3a and MLP-3b were acidic polysaccharides. Furthermore, crude MLP and MLP-3a had more complicated monosaccharide compositions, while MLP-3b had a relatively higher content of uronic acid. Crude MLP, MLP-3a and MLP-3b exhibited potent Fe 2+ chelating power and scavenging activities on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide and 2,2′-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) radicals. The results suggested that MLP could be explored as natural antioxidant.
研究领域:
食品科学
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植物化学
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生物化学
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DOI
高影响因子
PHYTOMEDICINE
·
2026
·
IF 11.3
Yuanyuan Liu,Ruoyan Wu,Huijuan Li,Danfeng Pu,Xingang Wang,Shan Chen,Chenjie Jiang,Wangmin Hu,Liyun Wang,Xinhua Ye,Wenlong Huang,Xueqin Wang,Yun Hu,Shiqin Yuan,Yumeng Han,Jun Ye,Qing He,Lan Xu,Yuling Liu
Background Polycystic ovary syndrome (PCOS) is a neuroendocrine-metabolic disorder with no approved drugs. Hypothalamic-pituitary-ovarian (HPO) axis dysfunction is core pathogenesis, and metabolic heterogeneity complicates treatment. Existing medications rarely ameliorate PCOS comprehensively. Sangzhi alkaloids (SZ-A), an extract from Morus alba L. twigs approved for type 2 diabetes in China, have not been systematically explored for PCOS. Purpose To clarify the therapeutic effect and the mechanisms of SZ-A in PCOS. Study Design Animal studies, in vitro experiments and a placebo-controlled clinical trial. Materials and methods In vivo : Obese/non-obese PCOS rats received oral SZ-A 67, 100, 150 mg/(kg·d) for 21 days (positive controls: Diane-35, metformin). In vitro : Granulosa cell apoptosis induced by dihydrotestosterone was treated with SZ-A 25, 50, 100, 200 μg/mL. Clinical: 6-month placebo-controlled trial (128 PCOS patients; oral SZ-A 300 mg/d, n=88; placebo, n=40). Multi-omics/Western blotting explored HPO pathways. Results and discussion In rats, SZ-A (67 to 150 mg/kg/d, 21 days) dose-dependently reduced androgens, regularized estrous cycles, and improved ovarian morphology. In vitro, SZ-A (25 to 200 μg/mL) protected granulosa cells from apoptosis. In patients, 6-month SZ-A (300 mg/d) lowered free androgen index and improved menstrual regularity versus placebo. Mechanistically, SZ-A inhibited hyperandrogenism-induced granulosa cell apoptosis and regulated GnRH secretion and downstream signaling pathways. Conclusion SZ-A exerts therapeutic effects on PCOS by modulating HPO axis function through multiple pathways. Trial registration ChiCTR2200065097
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DOI
PubMed
高影响因子
FITOTERAPIA
·
2025
·
IF 2.6
Wenyuan Li,Lin Chen,Minpeng Li,Kaiye Peng,Xuemei Lin,Yifan Feng,Yun Zou,Xia Wu
In this study, the chemical constituents of branch bark and root bark of Morus alba L. were first qualitatively analyzed by UPLC-Q/TOF-MS. A total of 40 compounds were identified, including 20 flavonoids, 3 stilbenes, 1 phenolic acid, 1 alkaloid, 5 arylbenzofurans and 10 Diels-Alder adducts. Of these, 19 compounds were specific to the root bark, 10 to the branch bark, and 11 were common to both. Total phenolic and flavonoid contents were determined, and anti-inflammatory activities were evaluated. Ethyl acetate extracts of both significantly inhibited NO secretion, with the root bark showing a stronger effect than the branch bark. Chemical components of branch bark were further separated, and the anti-inflammatory activities of these isolated compounds were investigated. Eight compounds were isolated from the ethyl acetate extract of the branch bark of Morus alba L. . These compounds were able to inhibit the release of inflammatory factors, including NO, IL-6, and IL-1β, in a concentration-dependent manner. Among them, compounds SZP-1, SZP-6 and SZP-8 exhibited superior in vitro anti-inflammatory activity, comparable to the positive drug meloxicam at high concentrations. In addition, the quality standard of the branch bark of Morus alba L. was established for the first time according to the 2020 edition of the Chinese Pharmacopoeia. This study provides valuable insights into the medicinal value of the branch bark of Morus alba L. and promotes the comprehensive utilization of mulberry branch resources.
研究领域:
药理学
·
植物化学
·
分析化学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Pengfei Zhou,Xuping Wang,Pengzhan Liu,Jing Huang,Chen Wang,Mushui Pan,Zheshi Kuang
It is of great significance to develop green solvents for promoting green and sustainable technology. Deep eutectic solvents (DESs) as new extraction solvents are emerging in the search for efficient the extraction of bioactive compounds or drugs. In this study, twelve types of DESs including choline chloride-, betaine-, and l -proline-based DESs were investigated in combination with ultrasonic-assisted extraction (UAE) for the extraction of phenolic compounds from Morus alba L. leaves. Compared with conventional extraction system, DESs composed of choline chloride/citric acid showed more effective extraction yields (22.66 mg/g vs. 15.29 mg/g). The operation conditions were also optimized systematically. In addition, liquid–liquid extraction was an effective approach to recover the major phenolic compounds in the extracts. The recovery and recyclability of DESs were also evaluated. A broad extractability of phenolic compounds in the certain designed DESs systems was observed according to principal component analysis (PCA). The developed DESs can be an alternative procedure for the green and efficient extraction of phenolic compounds from natural sources.
研究领域:
植物化学
·
天然产物开发
·
绿色化学
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·2688
·
IF 4.927
Tao Chen,Fei-Fan Shuang,Qing-Yue Fu,Yu-Xiong Ju,Chen-Man Zong,Wei-Guo Zhao,Dong-Yang Zhang,Xiao-Hui Yao,Fu-Liang Cao
Mulberry (Morus albaL.) fruit is a fruit with nutritional and medicinal value. It is widely cultivated in different regions of China, which may result in differences in its chemical composition. In this research, 25 mulberry fruit samples from six provinces in China were investigated. The contents of anthocyanins were evaluated by high-performance liquid chromatography (HPLC). The contents of two main anthocyanins, cyanidin-3-O-glucoside (C3G) and cyanidin-3-O-rutinoside (C3R), ranged from 0.656 ± 0.006 mg/g to 4.367 ± 0.243 mg/g and from 0.226 ± 0.007 mg/g to 1.649 ± 0.013 mg/g, respectively. Additionally, the contents of total phenolic, total flavonoid, vitamin C, titratable acids, reducing sugars and antioxidant capacity (FRAP, DPPH, scavenging and hydroxyl radical scavenging activity) were also assessed. The results and principal component analysis showed that the Zhongsang 5801 variety from Sichuan, Dechang had the greatest health value with the highest active compound contents. Based on our analysis, the variety from Sichuan, Dechang is a high-quality plant source for mulberry fruit cultivation. This research provides a basis for the rational development and utilization of mulberry fruit resources in China.Keywords:mulberry fruit;varieties and regions;anthocyanin;chemical composition;antioxidant activity
研究领域:
农业
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食品科学
·
药理学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Cheng-Hai Yan,Fang-Hui Chen,Yu-Lu Yang,Li-Wei Shen,Xiao-Meng Xun,Zhi-Ang Zhang,Yu-Fan Zhan,You Shuai,Jun Wang
BACKGROUND With the requirements of environmental, cost and economic sustainability, new sources of alternative proteins in the livestock industry are receiving increasing attention. Mulberry ( Morus alba L.) leaves are a unique feed resource due to their high protein content and large availability. Therefore, mining sustainable protein suitable for the animal husbandry industry in sericulture resources could achieve a win-win situation. RESULTS The protein content in mulberry leaves is 232.10-386.16 g·kg -1 , and the mean value of crude fat content is 43.76±8.48 g·kg -1 , which has the advantages of protein content and energy. In addition, the average content of phytic acid in mulberry leaves is only 1.88±0.56 g·kg -1 , which makes it not inhibited in terms of nutrient absorption. Meanwhile, the digestibility of protein was Bean pulp> Sample 8≈ Alfalfa≈ Sample 13≈ Cottonseed meal> Fish meal, and the β -turn and particle size of mulberry leaf protein are more conducive to digestion in vitro . Furthermore, the protein of sample 13 had the richest essential amino acids (252.00 g·kg -1 ) and the highest essential amino acid index (EAAI), which was superior to conventional feed protein. In addition, the partial substitution of mulberry leaf protein (15%) significantly increased the EAAI value of conventional feed protein. However, to balance nutrition, it is necessary to combine mulberry leaf protein with other proteins to further broaden its application field. CONCLUSION Mulberry leaves are a new source of feed protein, which helps to alleviate the two major problems of mulberry resource surplus and feed protein resource shortage. This article is protected by copyright. All rights reserved.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）