Lanlan Nie,Rui Li,Jie Huang,Lingling Wang,Mengjie Ma,Chunyue Huang,Tong Wu,Renjie Yan,Xiao Hu
Five undescribed abietane diterpenoids , dracocephalumoids A–E, together with six known analogues were isolated from the aqueous EtOH extract of aerial parts of Dracocephalum moldavica L.. The structures were elucidated through extensive analysis of spectroscopic data , and their absolute configurations were established by Mosher's method and time-dependent density functional theory electronic circular dichroism (TDDFT ECD) calculation. Cell-based anti-inflammatory assays displayed that dracocephalumoid A, uncinatone, trichotomone F and caryopterisoid C showed potent suppressive effects against TNF- α , IL-1 β or NO production in LPS-induced RAW 264.7 cells, with IC 50 values ranging from 1.12 to 5.84 μM. The structure-activity relationship of these compounds was also discussed.
Under guidance of 1 H NMR, ten new polypropionate derivatives, decempyrones A–J ( 1 – 10 ) along with two known analogues ( 11 and 12 ), were isolated from the marine-derived fungus Fusarium decemcellulare SYSU-MS6716. The planar structures were elucidated on the basis of extensive spectroscopic analyses (1D and 2D NMR, and HR-ESIMS). The absolute configuration of the chiral centers in the side chain is a major obstacle for the structure identification of natural polypropionate derivatives. Herein, the J -based configurational analysis (JBCA), chemical degradation, geminal proton rule, and the modified Mosher’s method were adopted to fix their absolute configurations in the side chain. Compounds 3 and 10 exhibited potent anti-inflammatory activity by inhibiting the production of NO in RAW264.7 cells activated by lipopolysaccharide with IC 50 values 22.4 ± 1.8 and 21.7 ± 1.1 μM. In addition, compounds 3 and 10 displayed MptpA inhibitory activity with an IC 50 value of 19.2 ± 0.9 and 33.1 ± 2.9 µM. Structure-activity relationships of the polypropionate derivatives were discussed.
危险品化学品经营许可证（带存储）