Algal Research-Biomass Biofuels and Bioproducts
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2021
·
IF 4.401
Pingkang Qian,Kun Gao,Zheng Gao,Chunye Xue,Xiaoli Hu,Qiaoqiao Liu,Jingwei Dong,Xiangyuan Deng
The aim of this work was to investigate the effects of myo -inositol (MI) on growth, photosynthetic activity , and chemical compositions of Chlorella sorokiniana . Results showed that a maximum value of cell density was obtained when this alga was cultivated in BG11 media with 1000 mg L −1 MI, which was 1.37 times greater than that in the controls. Moreover, it was observed that MI had a negative effect on algal photosynthetic activity, which could be alleviated by regulating the contents of chemical compositions, such as chlorophyll a (Chl a ). In the BG11 media with MI, contents of Chl a in the algal cells were in the range of 0.30–0.35 μg 10 −6 cells, which were about 1.75 times greater than that in the controls during microalgae cultivation. The accumulated Chl a would be used to capture and deliver more photons during algal photosynthesis . In addition, contents of lipids , carbohydrates, and proteins varied with the changes in MI concentrations. Thus, it is concluded that MI could promote the algal growth and play an important role in regulating the biochemical compositions in algal cells. These data would provide basic theory and technical support for large-scale cultivation and biomass production of microalgae by adding MI to culture media.
研究领域:
水生生物学
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生物化学
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DOI
高影响因子
POLYMER
·
2025
·
IF 4.5
Haoting Yin,Dongyuan Ye,Tianyu Wu,Xiaoyu Meng,Hai-Mu Ye
The sluggish crystallization kinetics of poly(ethylene succinate) (PES), a biodegradable aliphatic polyester with promising mechanical properties, severely restricts its industrial processing and application. To address this limitation, this study introduces two bio-derived hexahydric alcohols, myo -inositol (MIS) and dipentaerythritol (DPE), as nucleating agents to regulate PES crystallization. Non-isothermal and isothermal differential scanning calorimetry, polarized optical microscopy, and wide angle X-ray diffraction analyses reveal that both MIS and DPE significantly enhance nucleation density and crystallization rates via epitaxial templating and hydrogen-bonding interactions. DPE outperforms MIS, achieving 19.1 °C increase in crystallization temperature and 28% improvement in relative crystallinity at 2 wt% loading, attributed to its superior epitaxial surface provision and stronger intermolecular interactions for PES chains. Fourier transform infrared spectroscopy further confirms the plasticizing effect of these agents, which reduces rigid amorphous fractions and enhances chain mobility. The synergistic mechanism combines heterogeneous epitaxial nucleation and chain lubrication, enabling accelerated crystallization kinetics without compromising biodegradability. This work provides a sustainable strategy to optimize PES processing efficiency and performance, advancing its potential as a viable alternative to conventional thermoplastics.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2017
·Vol. 134
·期 16
·
IF 1.86
Hui Shi,Xuan Chen,Wenkai Chen,Sujuan Pang,Lisha Pan,Nai Xu,Tan Li
In this work, use of myo -inositol as a biobased nucleating agent (NA) for PLLA was researched. Effects of myo -inositol on non-isothermal and isothermal crystallization behaviors of PLLA at temperatures ranged from 85 °C to 130 °C were studied by using DSC, POM and WAXD. Isothermal crystallization kinetics results showed that the incorporation of myo -inositol enhanced significantly the crystallization rate of the PLLA samples. It was confirmed that the optimum isothermal crystallization temperature range was 100 to 110 °C. The above results were instructive to confirm proper heat treatment time and temperature for compression or injection molding to fabricate highly crystallized PLLA articles. The relations among heat treatment time, crystallinity, heat resistance, and mechanical performances of the neat PLLA and PLLA/1% myo -inositol specimens prepared by compression molding were investigated. Compared with the PLLA specimens, the PLLA/1% myo -inositol specimens showed a shorter heat treatment time to reach the maximum crystallinity. Vicat softening temperature, as well as tensile strength, modulus, and toughness of the PLLA/1% myo -inositol specimens was improved when crystallinity increased from 5.4% to 38.1%. Considering the nontoxicity and biocompatibility of myo -inositol, PLLA/ myo -inositol blends would be potential to prepare some products, which are required higher health standard and can be used in elevated temperature environments. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44732.
研究领域:
材料科学
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高分子化学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2025
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IF 3.7
Haotian Cheng,Yuan Li,Yi Zhou,Shuke Wu,Yi-Heng P. Job Zhang
Cellobiose phosphorylase (CBP) catalyzes the reversible phosphorolysis of cellobiose to glucose and α-D-glucose-1-phosphate. CBPs play an important role in the in vivo enzymatic utilization of cellulosic materials. Here we discovered a novel CBP from Thermoclostridium caenicola (TcCBP), displaying 50–75 % sequence homology with previously reported CBPs. Recombinant TcCBP was well expressed in E. coli BL21(DE3), with a 1.6-fold increase in soluble expression levels over the widely-used Clostridium thermocellum CBP (CtCBP). This enzyme exhibited broad pH adaptability, maintaining substantial activities across pH 4.0–7.5 in the synthetic direction and pH 5.0–7.5 in the phosphorolytic direction. Compared to CtCBP, TcCBP displayed the superior thermostability and a nearly 100-fold improvement in "Product-to-Enzyme Ratio" (PE value). The biosynthesis of myo -inositol from cellobiose was conducted by using this CBP along with other four thermophilic enzymes (i.e., phosphoglucomutase, inositol 1-phosphate synthase, inositol monophosphatase, and polyphosphate glucokinase) in one pot without step-by-step addition of enzymes. Approximately 96 mM of myo -inositol was produced from 50 mM of cellobiose. These results indicated that this enzyme could be a potential thermophilic enzyme used for the production of value-added biochemicals by in vitro BioTransformation.
研究领域:
酶工程
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分子生物学
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合成生物学
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代谢工程
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工业生物技术
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DOI
PubMed
高影响因子
Frontiers in Microbiology
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2020
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IF 4.236
Wang Xiaoshuai,Chen Lei,Liu Jing,Sun Tao,Zhang Weiwen
myo-inositol (MI) is an essential growth factor, nutritional source, and important precursor for many derivatives like D-chiro-inositol. In this study, attempts were made to achieve the “green biosynthesis” of MI in a model photosynthetic cyanobacterium Synechocystis sp. PCC 6803. First, several genes encoding myo-inositol-1-phosphate synthases and myo-inositol-1-monophosphatase, catalyzing the first or the second step of MI synthesis, were introduced, respectively, into Synechocystis. The results showed that the engineered strain carrying myo-inositol-1-phosphate synthase gene from Saccharomyces cerevisiae was able to produce MI at 0.97 mg L–1. Second, the combined overexpression of genes related to the two catalyzing processes increased the production up to 1.42 mg L–1. Third, to re-direct more cellular carbon flux into MI synthesis, an inducible small RNA regulatory tool, based on MicC-Hfq, was utilized to control the competing pathways of MI biosynthesis, resulting in MI production of ∼7.93 mg L–1. Finally, by optimizing the cultivation condition via supplying bicarbonate to enhance carbon fixation, a final MI production up to 12.72 mg L–1 was achieved, representing a ∼12-fold increase compared with the initial MI-producing strain. This study provides a light-driven green synthetic strategy for MI directly from CO2 in cyanobacterial chassis and represents a renewable alternative that may deserve further optimization in the future.
研究领域:
微生物学
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生物技术
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合成生物学
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DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
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2018
·
IF 2.975
Rui Zhang,Tianyun Li,Houbo Zhou,Huahua Huang,Yongming Chen
Here, we develope a kind of biobased transparent polyimides (PIs) with excellent solubility and mechanical properties by using myo-inositol derived diamines. Two biobased diamines M1 and M2 were synthesized through a protection reaction of –OH units of myo-inositol followed by the substitution and subsequent reduction reactions. Myo-inositol based PIs were prepared via the microwave-assisted polymerization of biobased diamines and commercial dianhydrides. These PIs were soluble in various common solvents even in CH 2 Cl 2 and acetone at room temperature . The biobased PIs also showed good optical transparency with T 450nm values of over 82%. Moreover, all the PI films possessed outstanding mechanical properties with tensile modulus of over 3.5 GPa, which was higher than that of most of petroleum-based PIs. In addition, the PI films containing –OH units were obtained via a simple deprotection reaction of myo-inositol based PIs.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
FLUID PHASE EQUILIBRIA
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2013
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IF 2.379
Yonghong Hu,Wenbiao Zhi,Mengmeng Liang,Zheng Cao,Wenge Yang
In this paper, we focused on solubility and solution thermodynamics of myo -inositol. By gravimetric method, the solubility of myo -inositol was measured in (water + acetic acid) binary solvent mixtures from 278.15 K to 333.15 K under atmosphere pressure. The solubility data were fitted using modified Apelblat equation, a variant of the combined nearly ideal binary solvent/Redich–Kister (CNIBS/R–K) model and Jouyban–Acree model. The three models were all proven to give good agreement with the experiment data, and modified Apelblat equation was the best. By means of van’t Hoff analysis, the enthalpy, entropy and Gibbs energy were calculated, which indicated that the dissolving process was nonspontaneous.
研究领域:
化学
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物理化学
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DOI
高影响因子
FOOD CHEMISTRY
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2023
·
IF 8.8
Xiaowei Wang,Kangli Li,Wei Zhao,Leida Zhang,Xuemei Wei,Runpu Shen,Mingyang Chen,Dandan Han,Junbo Gong
Myo-inositol, referred to as vitamin B8, is an essential nutrient for maintaining human physiological functions. However, the morphology of myo-inositol products is predominantly powder or needle shaped, leading to poor food properties. In this work, three edible sugar additives, i.e. d -glucose, l -arabinose and d -fructose, are adopted in the crystallization of myo-inositol to improve its food properties. The results show that these additives change the morphology of myo-inositol crystals. d -glucose and l -arabinose reduced the aspect ratio of myo-inositol crystals, and d -glucose transformed elongated lamellar myo-inositol crystals into diamond-shaped lamellar crystals. The diamond-shaped lamellar myo-inositol products exhibited outstanding functional food properties. It offered a smoother texture and more pleasant mouthfeel when the products were added to infant formulas and nutraceuticals. When they were applied to functional beverages, the dissolution rate was increased by 35 %. This work provides a theoretical guidance for improving food properties through crystallization and possesses considerable potential for industrialization.
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
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2022
·
IF 5.436
Linchen Xie,Anqi He,Da Li,Tianyi Li,Limin Yang,Kun Huang,Yizhuang Xu,Guozhong Zhao,Jingyu Liu,Kexin Liu,Jia’er Chen,Yukihiro Ozaki,Isao Noda
Single-crystal structures of myo-inositol complexes with erbium ([Er2(C6H11O6)2(H2O)5Cl2]Cl2(H2O)4, denoted ErI hereafter) and strontium (Sr(C6H12O6)2(H2O)2Cl2, denoted SrI hereafter) are described. In ErI, deprotonation occurs on an OH of myo-inositol, although the complex is synthesized in an acidic solution, and the pKa values of all of the OHs in myo-inositol are larger than 12. The deprotonated OH is involved in a μ2-bridge. The polarization from two Er3+ ions activates the chemically relatively inert OH and promotes deprotonation. In the stable conformation of myo-inositol, there are five equatorial OHs and one axial OH. The deprotonation occurs on the only axial OH, suggesting that the deprotonation possesses characteristics of regioselectivity/chiral selectivity. Two Er3+ ions in the μ2-bridge are stabilized by five-membered rings formed by chelating Er3+ with an O–C–C–O moiety. As revealed by the X-ray crystallography study, the absolute values of the O–C–C–O torsion angles decrease from ∼60 to ∼45° upon chelating. Since the O–C–C–O moiety is within a six-membered ring, the variation of the torsion angle may exert distortion of the chair conformation. Quantum chemistry calculation results indicate that an axial OH flanked by two equatorial OHs (double ax–eq motif) is favorable for the formation of a μ2-bridge, accounting for the selectivity. The double ax–eq motif may be used in a rational design of high-performance catalysts where deprotonation with high regioselectivity/chiral selectivity is carried out.
研究领域:
无机化学
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物理化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Tao Sun,Zhixiang Li,Shubin Li,Lei Chen,Weiwen Zhang
Cyanobacteria have been considered as an ideal photosynthetic chassis for CO 2 recycle and bioconversion, which could make a significant contribution to carbon neutrality. However, several inherited issues associated with current cyanobacterial strains need to be addressed before their biotechnological application, such as slow growth, uneven of carbon distribution, biased production of reducing power and imbalance between growth and biosynthesis. To address the issues, myo -inositol (MI) was selected as an example target and a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 was utilized as chassis to improve cell growth. Second, several genes involved in MI synthesis were evaluated. Third, the MI biosynthesis was further increased by cofactors enhancement and down regulation of the competing pathways using multiplex artificial small RNAs (PTRNAs). Forth, a MI sensor was developed and optimized to trigger the automatic expression PTRNAs to achieve real-time intelligent regulation, avoiding the growth defect caused by blocking essential genes at early growth stage. Fifth, carbon re-direction was achieved using rhythmical cultivation, resulting in a final production of 262.6 mg/L MI in shaking flask, which represents the highest production from CO 2 in cyanobacteria. Finally, the metabolomic analysis was applied to elucidate the shifted metabolic flux and predicted targets for future optimization. Together of all efforts, we can achieve the CO 2 -based MI biosynthesis to the same order of magnitude as heterogenous microorganisms with suitable scale-up in the future.
研究领域:
生物技术
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微生物学
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代谢工程
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合成生物学
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DOI
高影响因子
MicrobiologyOpen
·
2018
·Vol. 8
·期 5
·e00721
·
IF 2.682
Can Chen,Keqi Chen,Tao Su,Bing Zhang,Guizhi Li,Junfeng Pan,Meiru Si
Graphical The ino-1 deletion mutant showed obviously higher ROS levels than the WT(pXMJ19) and the complement Δino-1(pXMJ19-ino-1) strains under oxidative stress conditions. And clearer protein carbonyl groups were detected in the Δino-1(pXMJ19) strains after exposure to ROS-generating agents compared to that in the WT(pXMJ19) strains. Reactive oxygen species (ROS) generated in aerobic metabolism and oxidative stress lead to macromolecules damage, such as to proteins, lipids, and DNA, which can be eliminated by the redox buffer mycothiol (AcCys-GlcN-Ins, MSH). Myo -inositol-phosphate synthase (Ino-1) catalyzes the first committed step in the synthesis of MSH, thus playing a critical role in the growth of the organism. Although Ino-1s have been systematically studied in eukaryotes, their physiological and biochemical functions remain largely unknown in bacteria. In this study, we report that Ino-1 plays an important role in oxidative stress resistance in the gram-positive Actinobacteria Corynebacterium glutamicum . Deletion of the ino-1 gene resulted in a decrease in cell viability, an increase in ROS production, and the aggravation of protein carbonylation levels under various stress conditions. The physiological roles of Ino-1 in the resistance to oxidative stresses were corroborated by the absence of MSH in the Δ ino-1 mutant. In addition, we found that the homologous expression of Ino-1 in C. glutamicum yielded a functionally active protein, while when expressed in Escherichia coli BL21(DE3), it lacked measurable activity. An examination of the molecular mass (Mr) suggested that Ino-1 expressed in E. coli BL21(DE3) was not folded in a catalytically competent conformation. Together, the results unequivocally showed that Ino-1 was important for the mediation of oxidative resistance by C. glutamicum .
研究领域:
微生物学
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分子生物学
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生物化学
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细菌生理学
·
氧化应激
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Dengliang Cai,Guangxin Zhao,Wenan Tie,Ting Lei,Jiachun Feng
Polypropylene (PP) combines excellent mechanical properties with cost-effectiveness, yet its practical applications are hindered by poor thermo-oxidative resistance and slow crystallization kinetics. While conventional antioxidants and nucleating agents can mitigate these issues, increasing safety concerns over synthetic additives have spurred interest in natural alternatives. However, natural antioxidants such as vitamin E (VE) often exhibit inadequate stabilization at low loadings, and few industrially available biobased nucleating agents were developed. Here, we reported that myo-inositol (Ins), a bioderived compound, acted as a dual-functional additive, simultaneously improving both the thermo-oxidative stability and crystallization behavior of VE-containing PP. Incorporating 0.2 wt % Ins into PP stabilized with 0.1 wt % VE dramatically extended the oxidation induction time from 7.5 to 45.3 min, indicating strong synergistic antioxidant activity. Furthermore, Ins enhanced crystallization kinetics, increasing the peak crystallization temperature by 5.4 °C (10 °C/min cooling rate) and reducing the half-crystallization time by 69% during isothermal crystallization at 120 °C compared to VE-only PP. The dual role of Ins was attributed to its function as a metal chelator and a heterogeneous nucleation agent, offering a sustainable strategy for high-performance PP modification.
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DOI
高影响因子
危险品化学品经营许可证（带存储）