CELL AND TISSUE RESEARCH
·
2021
·Vol. 386
·期 3
·661-677
·
IF 5.249
Tong Yan,Cheng Lu-Qiang,Li Xuan,Yu Xu-Peng,Shu Rui-Hao,Zhang Ji-Hong,Meng Qian,Qin Qi-Lian,Tang Kai,Xu Jian-Xiang,Zhang Huan
Determining the source of primary cells is conductive to enriching sufficient cells with immortal potential thereby improving the success rate of establishing cell lines. However, most of the existing insect cell lines are established by mixing and fragmentation of explants. At present, the origin of cell lines can only be determined according to the cultured tissues, so it is impossible to determine which cell types they come from. In this study, a new cell line designated IOZCAS-Myse-1 was generated from pupal ovaries of the migratory pest Mythimna separata by explant tissues to derive adherent cultures. This paper mainly shows the further descriptive information on the origin of primary cells in the process of ovarian tissue isolation and culture. Phospho-histone H3 antibody-labeled cells with mitotic activity showed that the rapidly developing somatic cells in vivo gradually stopped proliferation when cultured ex vivo. The primary cells dissociated outside the tissue originated from the lumen cells, rather than the germ cells or the follicular epithelium cells. The results suggest that the newly established cell line IOZCAS-Myse-1 had two possible sources. One is the mutation of lumen cells in the vitellarium, and the other is the stem cells with differentiation potential in the germarium of the ovarioles. Moreover, the newly established cell line is sensitive to the infection of Autographa californica multiple nucleopolyhedrovirus, responds to 20-hydroxyecdysone and has weak encapsulation ability. Therefore, the new cell line can be a useful platform for replication of viral insecticides, screening of hormone-based insecticides and immunology research.
研究领域:
昆虫学
·
细胞生物学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 79
·期 6
·2005-2016
·
IF 4.1
Zhen Tian,Ruichi Li,Shichang Cheng,Tong Zhou,Jiyuan Liu
BACKGROUND Mythimna separata is a notorious pest causing crop damages at the larval stage. Gaining insight into larval olfaction mechanisms would provide knowledge for olfaction-based management of M. separata larvae. RESULTS In the present research, ( Z )-11-hexadecenal ( Z 11-16: Ald), a major component of M. separata sex pheromone, was found to attract early-instar larvae of M. separata in a food context. Using a fluorescent binding assay, we found that M. separata general odorant binding protein 2 (MsepGOBP2) exhibited high binding affinity to Z 11-16: Ald. Further, silencing of MsepGOBP2 resulted in a sharp reduction of the response to Z 11-16: Ald, which could not be mitigated by increasing the concentration of Z 11-16: Ald. Additionally, we employed molecular dynamics-based approaches to unravel the interaction details between MsepGOBP2 and Z 11-16: Ald, specifically the binding of Z 11-16: Ald to MsepGOBP2. CONCLUSION Z 11-16: Ald is attractive to early-instar larvae of M. separata , and MsepGOBP2 is identified to be indispensable in the larval detection of Z 11-16: Ald. These results could aid in the development of olfaction-based methods for controlling M. separata in the larval stage. © 2023 Society of Chemical Industry.
研究领域:
昆虫学
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化学生态学
·
分子生物学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Ruige Yang,Xiaobo Huang,Zhiping Che,Yuanyuan Zhang,Hui Xu
Podophyllotoxin is isolated as a sustainable natural bioresource from the roots and rhizomes of Podophyllum hexandrum and Juniperus Sabina . In continuation of our program aimed at the discovery of biorenewable natural product-based pesticides, a series of 4α-acyloxy derivatives of 2'-chloro-5-bromopicropodophyllotoxin or 2',5-dibromopicropodophyllotoxin were synthesized by structural modification of podophyllotoxin, and evaluated as insecticidal and acaricidal agents against two serious agricultural pests, Mythimna separata Walker and Tetranychus cinnabarinus Boisduval. Among all derivatives, compounds 6a and 7a exhibited more promising insecticidal and acaricidal activities than podophyllotoxin, and the final mortality rates of 6a , 7a and 1 against M. separata (at 1 mg/mL) and T. cinnabarinus (at 0.5 mg/mL) were 50%/53.3%/36.7%, and 33%/41.8%/1.6%, respectively. This suggested that introduction of the acetoxy moiety at the C-4 position of 2'-chloro-5-bromopicropodophyllotoxin/2',5-dibromopicropodophyllotoxin was necessary for the insecticidal and acaricidal activities.
研究领域:
植物化学
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害虫管理
·
农业科学
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DOI
高影响因子
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
·
2020
·Vol. 23
·期 2
·111-118
·
IF 1.195
Che Zhiping,Yang Jinming,Sun Di,Tian Yuee,Liu Shengming,Lin Xiaomin,Jiang Jia,Chen Genqiang
Background: It is one of the effective ways for pesticide innovation to develop new insecticides from natural products as lead compounds. Quinine, the main alkaloid in the bark of cinchona tree as well as in plants in the same genus, is recognized as a safe and potent botanical insecticide
to many insects. The structural modification of quinine into 9R-acyloxyquinine derivatives is a potential approach for the development of novel insecticides, which showed more toxicity than quinine. However, there are no reports on the insecticidal activity of 9Racyloxyquinine derivatives
to control Mythimna separata. Methods: Endeavor to discover biorational natural products-based insecticides, 20 novel 9Racyloxyquinine derivatives were prepared and assessed for their insecticidal activity against M. separata in vivo by the leaf-dipping method at 1 mg/mL. Results:
Among all the compounds, especially derivatives 5i, 5k and 5t exhibited the best insecticidal activity with final mortality rates of 50.0%, 57.1%, and 53.6%, respectively. Conclusion: Overall, a free 9-hydroxyl group is not a prerequisite for insecticidal activity and C9- substitution
is well tolerated; modification of out-ring double-bond is acceptable, and hydrogenation of double-bond enhances insecticidal activity; Quinine ring is essential and open of it is not acceptable. These preliminary results will pave the way for further modification of quinine in the development
of potential new insecticides.
研究领域:
农药学
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植物化学
·
昆虫学
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DOI
PubMed
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
·
2021
·
IF 1.571
Zhi-Ping Che,Jin-Ming Yang,Song Zhang,Di Sun,Yue-E Tian,Sheng-Ming Liu,Xiao-Min Lin,Jia Jiang,Gen-Qiang Chen
Endeavor to discover biorational natural products-based insecticides, two series (27) of novel 9R/S-acyloxy derivatives of cinchonidine and cinchonine were prepared and assessed for their insecticidal activity against Mythimna separata in vivo by the leaf-dipping method at 1 mg/mL. Among all the compounds, especially derivatives 6l and 6o exhibited the best insecticidal activity with final mortality rates of 75.0% and 71.4%, respectively. Overall, a free 9-hydroxyl group is not a prerequisite for insecticidal activity and C9-substitution is well tolerated; the configuration of C8/9 position is important for insecticidal activity, and 9S-configuration is optimal; 6’-OCH3 moiety is not necessary, removal of it is also acceptable.
研究领域:
有机化学
·
植物保护学
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·Vol. 109
·期 4
·e21859
·
IF 1.698
Yan Liu,Shuangyu Li,Hongjia Yang,Yaru Chen,Dong Fan
Carboxylesterases are one of the three major types of detoxification enzyme in insects. In this study, we screened 12 full-length carboxylesterase cDNA sequences from the oriental armyworm Mythimna separata ; they were named MsCarE1–MsCarE12 and registered in GenBank with accession numbers MK440541 – MK440552. Treatment of fourth instar larvae of M. separata with the LD 50 of the insecticide chlorantraniliprole increased the expression levels of MsCarE3 and MsCarE4 , while treatment with the LD 50 of lambda-cyhalothrin significantly increased the expression levels of MsCarE5 and MsCarE10 . Spatiotemporal expression detection showed that MsCarE3 , MsCarE4 , MsCarE5 , and MsCarE10 were expressed at different developmental stages and in different tissues of M. separata and their expression levels were different. Induction using a high dose of chlorantraniliprole resulted in lower expression of MsCarE3 and MsCarE4 . LD 50 of lambda-cyhalothrin induced higher expression of MsCarE5 and MsCarE10 , while LD 70 induced higher MsCarE10 expression at 3, 6, and 12 h after treatment. RNA interference successfully inhibited the expression of MsCarE3 , MsCarE4 , MsCarE5 , and MsCarE10 , to different degrees at different time points. Silencing of MsCarE5 , or MsCarE5 and MsCarE10 simultaneously changed carboxylesterase activity and increased the susceptibility of M. separata larvae to lambda-cyhalothrin. This study provides a new method to increase the insect susceptibility to insecticide. Research Highlights Twelve carboxylesterase cDNA sequences named MsCarE1–MsCarE12 were identified from Mythimna separata. Silence of MsCarE5, or MsCarE5and MsCarE10 simultaneously increased the insecticidal activity of lambda-cyhalothrin on M. separatalarvae.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
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PubMed
高影响因子
JOURNAL OF APPLIED ENTOMOLOGY
·
2016
·Vol. 141
·期 5
·363-371
·
IF 1.517
J. B. Fan,S. van de Velde,W. B. Sun,H. Yu,Y. L. Zhang,D. Wang
The oriental armyworm, Mythimna separata Walker (Lepidoptera: Noctuidae), is a severe pest of graminaceous crops in Asia and Australia. In this study, we investigated the impacts of Cry2Ab, cantharidin and its analogues (cantharidin-23 and cantharidin-24) on M. separata growth, hydrolytic enzymes and detoxifying enzymes. Differences in weight gain and enzyme activities among all treatments were observed. Larval and pupal weight gain and hydrolytic enzyme activities declined when larvae were treated with Cry2Ab, cantharidin and its analogues, individually or in combination. The combination of Cry2Ab and cantharidin or cantharidin-24 had a markedly adverse effect on larval weight gain. Alkaline phosphatase and acid phosphatase were inhibited, whereas glutathione S-transferase was upregulated in all treatments with sublethal doses. The maximum reduction in alkaline phosphatase activity and increase in glutathione S-transferase activity occurred after the larvae were treated with a combination of Cry2Ab and cantharidin-24 over 48 h. The results suggest that the compositions of Cry2Ab, cantharidin and cantharidin-24 have a potential use in pest management.
研究领域:
昆虫学
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植物保护
·
生物化学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·
IF 3.44
Xin-Ru Li,Yue Li,Wei Wang,Ning He,Xiao-Ling Tan,Xue-Qing Yang
Lambda-cyhalothrin has long been recommended as an effective insecticide to control the oriental armyworm , Mythimna separata (Walker), a notorious migratory pest of agricultural plants. Previous researches have suggested that survival, development, and reproduction of insects are influenced by sublethal concentrations of insecticides. However, the effects of sublethal concentrations of lambda-cyhalothrin on M. separata are less known. In this study, we determined the toxicity and effects of LC 20 and LC 50 concentration of lambda-cyhalothrin on development and reproduction of M. separata . Results indicate that LC 20 of lambda-cyhalothrin tends to decrease the life traits of M. separate , with a shortening larvae period of offspring and oviposition period, whereas LC 50 of lambda-cyhalothrin stimulates daily maximal fecundity and forwards the oviposition peak, suggesting a stimulation of reproduction by LC 50 of lambda-cyhalothrin. The M. separata population was increased by an LC 50 concentration of lambda-cyhalothrin, resulting in a net reproductive rate ( R 0 ) and intrinsic rate of increase ( r m ) significantly higher than that of the control. Transcripts of vitellogenin ( MsVg ) and vitellogenin receptor ( MsVgR ) genes were suppressed at day 1 after emergence of moth which developed from the larvae exposed to LC 20 and LC 50 of lambda-cyhalothrin, but were significant induced when the moth begin to lay eggs (day 4), with a more remarkable induction by LC 50 of lambda-cyhalothrin than those of LC 20 of lambda-cyhalothrin. Our results indicate that the observed stimulation of reproduction is therefore the results of up-regulation of MsVg and MsVgR by LC 50 of lambda-cyhalothrin.
研究领域:
昆虫学
·
毒理学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
·
2020
·
IF 1.345
Zhi-Ping Che,Jin-Ming Yang,Xi-Jie Shan,Yue-E Tian,Sheng-Ming Liu,Xiao-Min Lin,Jia Jiang,Mei Hu,Gen-Qiang Chen
A series of sulfonate derivatives of sesamol were synthesized and evaluated for their insecticidal activity against a crop-threatening agricultural pest, the pre-third-instar larvae of Mythimna separata in vivo. Among all the target compounds, compounds 3b, 3g, 3h, and 3p exhibited more promising insecticidal activity than sesamol and toosendanin, and the final mortality rates (FMRs) of 3b, 3g, 3h, 3p, 1, and toosendanin were 60.7%/60.7%/67.9%/53.6%/32.1%/50.0%, respectively. Especially compound 3h exhibited the most potent insecticidal activity with FMRs of 67.9%. This suggested that a 4-fluorophenylsulfonyl group introduced at the hydroxyl position of sesamol was necessary for obtaining the most potent compound.
研究领域:
植物化学
·
农业昆虫学
·
有机合成化学
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DOI
PubMed
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
·
2023
·
IF 1.7
Bo Zhou,Lu-Lu Liu,Wang-Yu Zhao,Li-Juan Han,Ai-Juan Li,Chun Zhao,Wen-Jun Wu,Ji-Wen Zhang
A series of 4-(propargyloxy) benzenesulfonamide derivatives with different substituents on the benzene ring were synthesized and evaluated for their insecticidal activity. Some of the compounds showed good insecticidal activity against Mythimna separata, and the LC50 value of the most active compound B2.5 was 0.235 mg/ml. Ultrastructural changes in the midgut epithelial cells of Mythimna separata were observed using transmission electron microscopy, and severe structural damage was found in microvilli, mitochondria and rough endoplasmic reticulum. It indicates that the possible site of action of these benzenesulfonamides is the cytoplasmic membrane and endomembrane system of the midgut epithelial cells. The above provides a basis for the development of novel insecticidal active compounds with a novel mechanism of action.
研究领域:
农药化学
·
昆虫生理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2025
·
IF 2.4
Tang Rui,Wei Dandan,Luo Chun-Hua,Qin Juan-Rong,Mobarak Syed-Husne,Yu Xing-Lin,Liu Tong-Xian
Global temperature increases require the reevaluation of pesticide efficacy under thermal stress conditions; however, most studies have focused on acute high-temperature exposure, neglecting the effects of natural diurnal temperature fluctuations and cumulative acclimation. This study investigated the effect of thermal acclimation during developmental on the sensitivity of Mythimna separata (Walker) (Lepidoptera: Noctuidae) to emamectin benzoate (EB). Larvae were reared under cyclic thermal regimes (25→34/38→25 °C), experiencing daily high-temperature exposures of 2, 4, or 6 hours from the egg stage. Continuous fluctuating high-temperature exposure did not have a detrimental effect on the life-history traits of M. separata but substantially increased its sensitivity to EB. Daily 6-hour exposure to either 34 °C or 38 °C significantly increased LC10 EB-induced mortality and reactive oxygen species (ROS) levels compared to constant 25 °C. Increasing thermal intensity progressively suppressed the levels of heat shock proteins (MsHsp70 and MsHsp90) and the enzymatic activities of superoxide dismutase and glutathione-S-transferase in the EB-exposed larvae. A pronounced temperature-dependent increase in cytochrome P450 monooxygenases (P450s) activity accompanied this suppression, suggesting an adaptive metabolic response that could accelerate the development of EB resistance under climate warming scenarios. Thus, thermal acclimation during insect development increases EB toxicity, potentially through ROS accumulation caused by inhibiting heat shock proteins and antioxidant enzymes. These findings demonstrate that fluctuating thermal conditions amplify EB toxicity and underscore the potential role of P450s in long-term resistance to global temperature increases. This study guides the development of climate-adaptive pesticide applications and region-specific integrated pest management strategies.
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PubMed
高影响因子
ENVIRONMENTAL ENTOMOLOGY
·
2025
·
IF 1.5
Meng Wei,Zhang Wanming,Zhang Dan,Zheng Jingjun,Huang Dechao,Guo Qianshuang,Du Yongjun
The sex pheromone of the oriental armyworm, Mythimna separata (Walker), in China has always been confusing. It was initially considered as cis-11-hexadecenal (Z11-16:Ald) or cis-11-hexadecenyl acetate (Z11-16:Ac) and cis-11-hexadecenol (Z11-16:OH), as well as a mixture of these compounds. However, inconsistent results have frequently occurred using these compounds or mixtures in field trapping. Through gland analysis from individual females and nationwide field experiments, we identified two distinct types: Z11-16:Ald (aldehyde type or A type) and a mixture of Z11-16:Ac and Z11-16:OH (ester type or E type). Electroantennography (EAG) results indicated that both strains of males co-existed in the field. A strain of males had a strong EAG response to Z11-16:Ald but weak responses to Z11-16:Ac and Z11-16:OH, whereas E strain males had strong responses to Z11-16:Ac and Z11-16:OH but a weak response to Z11-16:Ald. Z11-16:OH identified in female E strain inhibited the attraction of A strain males to A type pheromones. Both A and E types of sex pheromone were respectively formulated and field evaluation (2014-2021) across > 20 provinces from southern to northern China showed geographic and generational variations. During overwintering, E strain males were predominantly caught in Hainan, Fujian, and Guangdong provinces, while A strain males were mainly trapped in Guangxi, Anhui, Sichuan, Jiangsu, and Jiangxi provinces. Subsequent generations exhibited mixed populations in most regions. These findings suggest regional adaptation and migration-driven hybridization. The study highlights the necessity of strain-specific pheromone blends for effective monitoring and control. This work resolves long-standing ambiguities in M. separata sex pheromone ecology.
研究领域:
昆虫学
·
化学生态学
·
信息素研究
·
种群遗传学
·
农业害虫治理
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）