Marine Drugs
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2022
·Vol. 20
·期 11
·719
·
IF 6.085
Qian Wang,Fu-Jun Liu,Xin-Miao Wang,Guan-Hua Zhao,Dong Cai,Jing-Han Yu,Fa-Wen Yin,Da-Yong Zhou
Low molecular weight (<5 kDa) peptides from mussels (Mytilus edulis) (MPs) and the peptides from clams (Ruditapes philippinarum) (CPs) were prepared through enzymatic hydrolysis by proteases (dispase, pepsin, trypsin, alcalase and papain). Both the MPs and the CPs showed excellent in vitro scavenging ability of free radicals including OH, DPPH and ABTS in the concentration range of 0.625–10.000 mg/mL. By contrast, the MPs hydrolyzed by alcalase (MPs-A) and the CPs hydrolyzed by dispase (CPs-D) had the highest antioxidant activities. Furthermore, MPs-A and CPs-D exhibited protective capabilities against oxidative damage induced by H2O2in HepG2 cells in the concentration range of 25–800 μg/mL. Meanwhile, compared with the corresponding indicators of the negative control (alcohol-fed) mice, lower contents of hepatic MDA and serums ALT and AST, as well as higher activities of hepatic SOD and GSH-PX were observed in experiment mice treated with MPs-A and CPs-D. The present results clearly indicated thatMytilus edulisandRuditapes philippinarumare good sources of hepatoprotective peptides.Keywords:Mytilus edulis;Ruditapes philippinarum;hepatoprotective peptide;H2O2-induced oxidative damage;mice;acute liver injury
研究领域:
生物化学
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药理学
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分子生物学
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Jia-Nan Chen,Xu-Hui Huang,Jie Zheng,Yi-Han Sun,Xiu-Ping Dong,Da-Yong Zhou,Bei-Wei Zhu,Lei Qin
Blue mussel ( Mytilus edulis L.) is a shellfish with high nutritional and economic value. Free amino acids (FAAs), 5′-nucleotides, and lipids that affected the nutritional value and flavor of blue mussels varying with the seasons were analyzed using stable isotope labeling-liquid chromatography coupled with tandem mass spectrometry (SIL-LC-MS/MS) and ultra-performance liquid chromatography-Q-Exactive orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). FAAs and 5′-nucleotides were most in the summer. Changes in FAAs between seasons were more obvious than those between genders, except in spring, when females were in the reproductive period. A total of 413 lipids were annotated in blue mussels for one year. Seasonal variation in triacylglycerols (TAGs) was the most evident. TAGs were dominant in females, while phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) were dominant in males. Overall, blue mussels in summer were more delicious and nutritious. Thus, such findings are of great significance to the seasonal preference of deep processing and consumption of mussel products.
研究领域:
海洋生物学
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营养学
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代谢组学
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脂质组学
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食品科学
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高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Li-Xin Ma,Xu-Hui Huang,Jie Zheng,Liu Dong,Jia-Nan Chen,Xiu-Ping Dong,Da-Yong Zhou,Bei-Wei Zhu,Lei Qin
Blue mussel ( Mytilus edulis L.) is a popular, nutritional, and tasty mollusk. To better understand the composition of nutrients and improve further processing of the mussels, metabolomic approaches were used to analyze the free amino acids, 5ʹ-nucleotides, and lipid compositions of different tissues. Our results showed that the viscera and gonad were rich in glutamine and glycine. Adenosine 5ʹ-monophosphate, uridine 5ʹ-monophosphate, guanosine 5ʹ-monophosphate, and inosine 5ʹ-monophosphate were abundant in the mantle, foot, and adductor muscle. Three main types of lipids, phospholipids (PLs), glycerides, and fatty acids (FAs), were semi-quantified. PLs were mainly distributed in the gonad of male mussels and viscera, gonad, and mantles of female mussels. FAs were relatively high in the viscera of males and in the gonad and viscera of females. The viscera of females were rich in phosphatidylcholine, such as 16:0/22:6 and 16:0/20:5. Triglycerides were the key lipids for distinguishing different tissues, especially 16:0/18:1/18:3 and 16:0/18:4/20:5.
研究领域:
海洋生物学
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营养学
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代谢组学
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食品科学
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PubMed
高影响因子
Advanced Materials Interfaces
·
2023
·Vol. 10
·期 20
·2300252
·
IF 5.4
Lingyun Xu,Zhihao Zhao,Yinghao Ge,Zhixin Wang,Xiaohan Sun,Xiangyu Jiang
Assembling functional materials into one-dimensional arrays is an efficient method for the integration of devices. However, the complicated assembly methods and poor stability of patterns still restrict their further applications. In this work, the well-dispersible nanosheets are prepared by introducing a bionic adhesive strategy. The oriented arrays with uniform morphology and precise position are realized by the liquid-film-induced capillary bridge assembly method. Owing to the abundant interactions between catechol structure and targeted substrates, the micro-wires present extraordinary stability in adhesion tests. Field-effect transistors (FETs) based on these micro-wires are also fabricated, and enhanced carrier mobility is achieved, exhibiting to be 41 times higher than pristine film-structured FETs. Micro-wires have a certain stability and improved electrical properties at the same time, which endows this strategy with practicality and application prospect.
研究领域:
材料科学
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生物仿生学
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纳米技术
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Zhi Li,Lunyu Zhang,Kui Jiao,Yuxi Wei,Fei Li
This study aimed to investigate the stability, hypotensive effects, and underlying mechanisms of Mytilus edulis -derived angiotensin-converting enzyme inhibitory peptides (MEPs-F) in spontaneously hypertensive rats (SHRs). The results demonstrated that MEPs-F exhibited remarkable stability under thermal treatment, various pH conditions, high-salt exposure, and simulated gastrointestinal digestion. The MEPs-F(H) group produced maximum short-term reductions of 49 mmHg in systolic blood pressure (SBP) and 71 mmHg in diastolic blood pressure (DBP), and the maximum long-term reductions reached 85 and 65 mmHg (SBP and DBP) compared to the SHR group. Moreover, MEPs-F-treated groups increased the level of the vasodilatory mediator nitric oxide (NO) and decreased the secretion of the potent vasoconstrictor endothelin-1 (ET-1), thereby rectifying the impaired NO/ET-1 equilibrium in SHRs. The antihypertensive mechanisms of MEPs-F were mediated through the dual modulation of the renin-angiotensin-aldosterone system (RAAS) and the TGF-β/Smad signaling pathway. Specifically, it reduced blood pressure (BP) by inhibiting the ACE/Ang II/AT1 axis and concurrently activating the ACE2/Ang-(1–7)/Mas axis. Additionally, MEPs-F suppressed the TGF-β/Smad pathway via downregulating the protein expression levels of TGF-β, Smad2, Smad3, and Smad4, and upregulating the protein expression level of Smad7. MEPs-F-treated groups decreased the Firmicutes / Bacteroidetes (F/B) ratio and significantly reduced the abundance of Lactobacillus in SHRs, ameliorating the gut microbiota dysbiosis associated with hypertension. This study highlighted MEPs-F's potential as a promising functional food component with multi-organ protective mechanisms in hypertension management.
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高影响因子
Bioactive Materials
·
2025
·
IF 20.3
Zeyu Du,Yuting Huang,Qing Ma,Wentai Zhang,Yan Fu,Nan Huang,Xin Li,Zhilu Yang,Wenjie Tian
Surfaces with enduring and superior antithrombotic properties are essential for long-term blood-contacting devices. While current surface engineering strategies integrating anticoagulants and antiplatelet agents show promise in mimicking the non-thrombogenic properties of the endothelium, their long-term effectiveness remains limited. Here, we report an easy-to-perform, dual-biomimetic surface engineering strategy for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces. We first designed a Mytilus edulis foot protein-5 (Mefp-5) mimic rich in amine and clickable alkynyl groups to polymerize-deposit a chemical robust coating onto the surface through a mussel-inspired adhesion mechanism. Then, a clickable nitric oxide (NO, an antiplatelet agent)-generating enzyme and the anticoagulant heparin were sequentially co-grafted onto the chemical robust coatings via click chemistry and carbodiimide chemistry. Our results demonstrate that this engineered surface achieved an impressive NO catalytic release efficiency of up to 88 %, while heparin retained 86 % of its bioactivity even after one month of exposure to PBS containing NO donor. Both in vitro and in vivo experiments confirmed that this robust endothelium-mimicking coating substantially reduces thrombosis formation. Overall, our long-acting endothelium-mimicking anti-thrombotic coatings present a promising and feasible strategy to address thrombosis-related challenges associated with blood-contacting devices.
研究领域:
生物材料学
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医学工程
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生物化学
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PubMed
高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Zhe Xu,Zhixuan Zhu,Hui Chen,Lingyu Han,Pujie Shi,Xiufang Dong,Di Wu,Ming Du,Tingting Li
The IEELEEELEAER peptide (PIE) identified from the protein hydrolysate of Mytilus edulis is reported to enhance osteoblast growth and differentiation, which also possesses a superior bone formation ability both in vitro and in vivo . Moreover, PIE bound to calcium spontaneously at the stoichiometry of 1:1, and there were amino nitrogen and carboxyl oxygen atoms in 2 glutamic acid residues at the calcium-binding sites in the PIE. The PIE–calcium complex facilitated calcium uptake through the Caco-2 cell monolayers. Incorporation of PIE into calcium phosphate cements enhanced calcium ion uptake and proliferation of osteoblasts and inhibit bacteria. This study suggest that calcium phosphate cements supplemented with PIE can serve as a potentially efficient material for bone graft used during spinal surgery.
研究领域:
生物材料学
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骨科医学
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分子生物学
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PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 6
·3313-3320
·
IF 3.289
Yue Zhou,Qinggang Xu,Xinghua Zhou,Shuang Song,Beiwei Zhu
Bioactive peptides derived from mussels have multiple healthcare functions. Herein, we aimed to examine the effects of mussel peptide preparation on lifespan, stress resistance, apoptosis and aging in Caenorhabditis elegans. Lifespan was determined by counting the number of surviving nematodes daily. ROS level and lipofuscin were measured using a fluorescent microscope. The mussel protein was prepared and hydrolyzed, and then fractionated by ultrafiltration. The fraction (<3 kDa) was purified by gel filtration to obtain the bioactive peptides, and the peptide sequences included in the fractions were identified, which were mostly composed of peptides with <20 amino acid residues. Mussel peptides treatment was found to significantly increase oxidative stress resistance and extend the lifespan of C. elegans. Moreover, this treatment also reduced endogenous ROS level and aging pigments accumulation in C. elegans as well as apoptosis. Collectively, these findings demonstrated that mussel peptides could contribute to healthspan extension of C. elegans through regulating mRNA expression of daf-2 and daf-16. These results highlighted the important role of mussel peptides for food and pharmaceutical industries to develop new nutraceuticals and functional foods.
研究领域:
分子生物学
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老年学
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营养保健品
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功能性食品
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PubMed
高影响因子
Journal of Materials Chemistry B
·
2026
·
IF 6.2
Xinrong Cheng,Bicheng Liang,Guantian Li,Haoyue Yang,Kecheng Li,Song Liu,Huahua Yu,Ronge Xing
In mollusk shells, the chitin-based framework provides critical support for the attachment of shell-associated proteins and biomineralization. Our research has proposed that this chitin framework may also be closely associated with melanin deposition in the shell of Mytilus edulis. Using thermal acid hydrolysis, a melanin–chitin complex (M–C complex) was extracted from mussel shells. Subsequently, scanning electron microscopy and Micro-CT were employed to reveal the morphological and structural characteristics of this complex, showing that melanin tightly adheres to the chitin framework. Further characterization by FTIR and Raman mapping confirmed their co-localization. ssNMR and XPS analyses also provided some evidence that seems to suggest that hydrogen bonding may be a key factor mediating their interaction. Based on these, a novel possible melanin–chitin structural model was proposed. This discovery enhances our understanding of the shell architecture and provides crucial insights into the composition and formation mechanisms of shells.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Xinyu Qiao,Han Li,Yunjiao Ma,Xianbing Xu,Junjie Yi,Hesham R. El-Seedi,Ming Du
Aquatic protein hydrolysates have many biological activities, but the off-flavor seriously decreases their commercial acceptability. Therefore, it is important to invest in finding an effective deodorization of aquatic hydrolysates that do not affect activities. In this study, ethanol pretreatment of mussel was applied to establish a new method to deodorize the blue mussel ( Mytilus edulis L. ) hydrolysates. LC-MS and GC–MS analysis results showed that 87.34% of fatty acids, 83.94% of aldehydes, most volatile flavor compounds including aldehydes, ketones, alcohols, acids, and hydrocarbons were decreased after ethanol pretreatment. Besides, it was found that the enzymatic hydrolysates of mussel with or without ethanol pretreatment showed high osteogenic activity, which induced an increase of 33.65 ± 4.36% and 31.77 ± 5.45% in MC3T3-E1 cell growth. These results suggest that ethanol pretreatment has beneficial potential for improving the flavor aspects of blue mussel peptides which may have the potential to stimulate bone regeneration and formation.
研究领域:
海洋生物学
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食品科学
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生物技术
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PubMed
高影响因子
EUROPEAN JOURNAL OF NUTRITION
·
2026
·Vol. 65
·期 2
·52
·
IF 4.5
Liu Kaiyue,Li Na,Fan Jiayi,He Siliang,Tang Weixiang,Cao Yaxin,Shen Liang,Sun Shuyang,Du Chengcheng,Du Chao
Purpose This study investigated the basic composition, in vitro digestibility, biological activities, and potential bioactive peptides of Pacific oyster (PO), Mytilus edulis (ME), Argopecten irradians (AIS), and Meretrix meretri x L. (MML) after simulated gastrointestinal digestion (SGD). Methods SGD products were prepared using the INFOGEST 2.0 model, and their nutritional properties and biological activities were analyzed. A comprehensive peptidomic profiling of the digested samples was conducted using nano-liquid chromatography-tandem mass spectrometry (nano-LC MS/MS) analysis. Results All four shellfish exhibited a nutritional profile characterized by high protein and low-fat content. The digestibility of shellfish increased progressively, while the average molecular weight (MW) of the hydrolysates gradually decreased during SGD, with the intestinal hydrolysates showing the highest overall digestibility. Biological activity analysis demonstrated that intestinal digestion products from all four species exhibited notable ABTS radical scavenging activity and α-glucosidase inhibition activity, with PO and ME exhibiting the strongest effects. A total of 349, 99, 314 and 105peptides were identified from the digestion products of PO, ME, AIS, and MML, respectively. Among these, PO and AIS showed the highest sequence similarity, sharing 69 identical peptides. By contrast, MML displayed distinct peptidomic characteristics, including a higher proportion of large MW peptides. Conclusion This study provides a comprehensive peptide catalog of shellfish digestion products and offers new insights into the biological activities of shellfish-derived peptides, supporting their potential as functional food resources.
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Marine Drugs
·
2025
·Vol. 23
·期 8
·294
·
IF 5.4
Jing Lu,Pujing Shi,Yutian Cao,Bingxin Shi,Huilin Shen,Shuai Zhao,Yuchen Gao,Huibing Chi,Lei Wang,Yawei Shi
Mussels are nutrient-rich but perishable, resulting in substantial resource loss. A protease-producing strain (Bacillus velezensisZ-1,Mytilus edulis) isolated from marine sludge was used to hydrolyze mussels, producing Y-1, a hydrolysate with antioxidant activity. In this study, ultrafiltration, gel chromatography, and LC-MS/MS were employed to isolate and identify bioactive peptides from the hydrolysate. The results revealed that the hydrolysate exhibited antioxidant activity, pancreatic cholesterol esterase inhibitory activity, pancreatic lipase inhibitory activity, and α-glucosidase inhibitory activity. Molecular docking using AutoDock Tools 1.5.6 was performed to analyze the interactions of peptides with CD38 and Keap1, leading to the identification of five potentially bioactive peptides: VPPFY, IMLFP, LPFLF, FLPF, and FPRIM. These peptides formed hydrogen bonds and hydrophobic interactions with CD38 and Keap1, demonstrating strong DPPH radical scavenging and superoxide anion radical scavenging capacities. This study highlights the multifunctional bioactive potential of these peptides, offering insights into their therapeutic applications. The findings provide a novel approach for the effective utilization of mussel resources and highlight their potential application value in the development of functional foods.
研究领域:
海洋生物技术
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食品科学
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生物化学
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药理学
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生物技术
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PubMed
高影响因子
危险品化学品经营许可证（带存储）