MATERIALS CHEMISTRY AND PHYSICS
·
2018
·
IF 2.21
Yi Wang,Yuan Zhong,Li Luo,Xiuhua Li,Tieying Yin,Junli Huang,Xiaojuan Zhang,Wei Wu,Guixue Wang
The appropriate composite ratio of cationic polymer and gene is fundamental but paramount determinant for efficient gene condensation . In this study, we proposed to construct a novel strategy to comprehensively understand gene condensation situation by using the pyrene (Py) modified polyethylenimine as fluorescent self-reporter, owing to the unique monomer and excimer fluorescence of Py groups highly depending on their intermolecular interval. Constant with the traditional measurements for polymer-gene complex including gel retardation analysis, DLS, ζ-potential, and TEM , the typical curve of fluorescence intensity ratio at I 380 / I 490 was verified to easily have an insight into the gene condensing process, and further recommend the appropriate range of N/P ratio (the ratios of moles of the amine groups of cationic polymers to those of the phosphate ones of DNA) for enhancing gene delivery. Thus, the facilely fabricated fluorescigenic cationic polymer based on an unique excimer-monomer switch could be used as an novel fluorescent self-reporter for comprehensively discerning the gene condensation, which may provide a feasible way to improving the optimal gene encapsulation for efficient and safe gene delivery.
研究领域:
生物技术
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基因治疗
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Shuze Ren,Songming Li,Mahmoud Abou-Elsoud,Rui Cui,Xing Fu,Xi Huang
Phosvitin phosphopeptides (PPP) have a high ability to chelate mineral ions and promote calcium absorption. In this study, The Fe 3 O 4 nanospheres were first synthesized by hydrothermal method, then the three magnetic nanoparticles (Fe 3 O 4 @PDA@PMAA@PAMAM, Fe 3 O 4 @PDA@Ce 4+ , and Fe 3 O 4 @PDA@TiO 2 ) were obtained by layer modification on their surfaces, respectively. The magnetic nanoparticles were characterized by TEM, XRD, VSM, and Zeta potential. TEM analysis revealed that the nanoparticles were spherical and regular in shape. XRD diffraction analysis showed that the modified nanoparticles have a complete crystal structure. Meanwhile, PAMAM, Ce 4+ , TiO 2 , and other groups have been successfully modified on the nanoparticles. VSM hysteresis regression line results revealed that the magnetic nanoparticles are superparamagnetic. The three kinds of magnetic nanoparticles still maintained excellent enrichment performance after five times of repeated use. Fe 3 O 4 @PDA@TiO 2 has the best enrichment performance (167 mg g −1 ). The N/P ratio of the enriched PPP decreased from 1.36 to 0.98, which also showed that it had excellent PPP enrichment performance. Therefore, the modified magnetic nanoparticles could be an optimal PPP enrichment and purification material.
研究领域:
材料科学
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纳米技术
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
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2011
·Vol. 9
·期 22
·7799-7806
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IF 3.451
Wenyu Li,Lina Chen,Zhixiong Huang,Xiaofei Wu,Yuanfeng Zhang,Qiaoling Hu,Youxiang Wang
Cyclodextrin-modified polycations have been studied widely due to their low cytotoxicity, low immunogenicity and the ability to form inclusion complexes. However, the influence of CD modification on cellular uptake and transfection efficiency of polyplexes is still unclear. In this research, cyclodextrin-modified polyethylenimines (PEI-CD) with different CD-grafting levels were synthesized, which were named PEI-CD15 and PEI-CD41, respectively, according to the CD number per PEI chain. CD modification showed great influence on the DNA condensation ability of the polycation. PEI-CD15 could protect DNA completely above N/P ratio of 2. The particle sizes of these polyplexes were about 120 nm. However, PEI-CD41 could not protect DNA below N/P of 6, and PEI-CD41/DNA polyplexes were larger than 1 μm, even at N/P ratio of 10. Therefore, this research was mainly focused on PEI-CD15. It was interesting that the PEI-CD15/DNA polyplexes at N/P ratio of 8 and 10 displayed excellent stability in physiological salt conditions, probably due to the hydration shell of CDs. The influence of CD modification on the cellular uptake and transfection efficiency of polyplexes depended on the type of the cells. Uptake inhibition experiments indicated that PEI/DNA polyplexes were internalized by HEK293T cells by both clathrin-mediated endocytosis and caveolae-mediated endocytosis. The route of caveolae-mediated endocytosis was significantly promoted after CD modification. So the cell uptake and transfection efficiency of PEI-CD15/DNA polyplexes were significantly improved for HEK293T cells. However, the uptake and transfection efficiency of PEI-CD15/DNA polyplexes in HepG2 cells was similar to that of PEI/DNA polyplexes, probably due to the lack of endogenous caveolins.
研究领域:
生物医学工程
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分子生物学
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基因治疗
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2019
·
IF 5.667
Yuxin Zhang,Jie Zhou,Shengnan Ma,Yiyan He,Jun Yang,Zhongwei Gu
Smart polymers as ideal gene carriers have drawn increasing attentions due to the effective DNA release once triggered by intrinsic stimuli, as well as reduced cytotoxicity. Herein, a stimulus-responsive, positively charged and water-soluble polymer (OEI-TKx) was facilely engineered by cross-linking low molecular weight oligoethylenimine (OEI) via thioketal (TK) linkages that would cleave selectively in reactive oxygen species (ROS)-rich environments induced by hypoxia. Agarose gel electrophoresis assay demonstrated that the threshold N/P ratios for complete retardation of negatively charged DNA migration were above 5 for OEI-TKx. The reduction in DNA-condensing capability and the changes in particle size, size distribution and particle morphology all illustrated that OEI-TKx possessed excellent ROS responsiveness. OEI-TKx/DNA polyplexes showed lower toxicity and higher gene transfection efficiency compared with PEI/DNA polyplexes. The optimum formulation, OEI-TKx/DNA polyplexes (N/P = 40), showed a little better performance than PEI/DNA polyplexes in cellular uptake profile. Furthermore, OEI-TKx/DNA polyplexes could escape from endosomes to the cytosol as efficiently as PEI/DNA polyplexes. Confocal images confirmed that OEI-TKx/DNA polyplexes could more effectively release DNA than PEI/DNA polyplexes, mainly owing to the valid cleavage of thioketal linkages induced by characteristic rich-ROS in Hela cells. These results suggested that OEI-TKx could represent an on-demand stimulus-responsive gene delivery platform.
研究领域:
生物医学工程
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药学
·
纳米技术
·
分子生物学
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）