PEST MANAGEMENT SCIENCE
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2023
·Vol. 79
·期 8
·2725-2736
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IF 4.1
Qi Li,Ning Zhao,Minghao Jiang,Mali Wang,Jingxu Zhang,Haiqun Cao,Min Liao
BACKGROUND Echinochloa glabrescens Munro ex Hook. f. is one of the main Echinochloa spp. seriously invading Chinese rice fields and has evolved resistance to commonly used herbicides. Previously, an E. glabrescens population (LJ-02) with suspected resistance to the acetyl-CoA carboxylase (ACCase)-inhibiting herbicide metamifop was collected. This study aimed to determine its resistance status to metamifop and investigate the internal molecular mechanisms of resistance. RESULTS Single-dose testing confirmed that the LJ-02 population had evolved resistance to metamifop. Gene sequencing and a relative expression assay of ACCase ruled out target-site based resistance to metamifop in LJ-02. Whole-plant bioassays revealed that, compared with the susceptible population XZ-01, LJ-02 was highly resistant to metamifop and exhibited cross-resistance to fenoxaprop- P -ethyl. Pretreatment with the known glutathione S -transferase (GST) inhibitor, 4-chloro-7-nitrobenzoxadiazole (NBD-Cl), largely reversed the resistance to metamifop by approximately 81%. Liquid chromatography–tandem mass spectrometry analysis indicated that the metabolic rates of one of the major metabolites of metamifop, N -(2-fluorophenyl)-2-hydroxy- N -methylpropionamide (HPFMA), were up to 383-fold faster in LJ-02 plants than in XZ-01 plants. There were higher basal and metamifop-inducible GST activities toward 1-chloro-2,4-dinitrobenzene (CDNB) in LJ-02 than in XZ-01. Six GST genes were metamifop-induced and overexpressed in the resistant LJ-02 population. CONCLUSION This study reports, for the first time, the occurrence of metabolic metamifop resistance in E. glabrescens worldwide. The high-level metamifop resistance in the LJ-02 population may mainly involve specific isoforms of GSTs that endow high catalytic activity and strong substrate specificity. © 2023 Society of Chemical Industry.
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高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Zhuwu Jiang,Yuchang Wang,Hai Yu,Ning Yao,Jyunhong Shen,Yan Li,HongYu Zhang,Xue Bai
With the rapid development of drinking water disinfection technology , extensive attentions are paid to the nitrogenous disinfection by-products (N-DBPs) that has strong carcinogenicity , thus their degradation becomes important for the health of human beings. In this work, for the first time, CoFe-LDH material used as particle electrode is proposed to treat trace N-nitrosopyrrolidine (NPYR) in a three-dimensional aeration electrocatalysis reactor (3DAER). The factors on the degradation efficiency and energy consumption of NPYR are systematically investigated, and the results of radical quenching experiments show that the degradation of NPYR is completed by combining with ·OH, ·O 2 and direct oxidation together. CoFe-LDH particle electrode plays a vital role in generating ·OH via heterogeneous ‾Fenton-like reaction. Moreover, the adsorbed saturated CoFe-LDH particle electrode can be regenerated by electrochemical action to induce further recycle adsorption and form in-situ electrocatalysis. This work pave a way for the removal of NPYR with high efficiency, low energy conservation and environmental protection.
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Agronomy-Basel
·
2023
·Vol. 13
·期 1
·216
·
IF 3.7
Guangtao Zhu,Hao Wang,Haitao Gao,Ying Liu,Jun Li,Zhike Feng,Liyao Dong
Italian ryegrass (Lolium multiflorumL.), a cross-pollinated grass, is gradually becoming a predominant weed in wheat fields in China and is evolving resistance to many groups of herbicides. The aim of this study is to determine the resistance levels of a singleL. multiflorumpopulation from a wheat field in Henan Province China, to three modes of action (MoAs) of herbicides and to further characterize the potential resistance mechanisms. ThisL. multiflorumpopulation evolved multiple herbicide resistances to pyroxsulam [acetolactate synthase (ALS)], pinoxaden [acetyl-CoA carboxylase (ACCase)] and isoproturon [photosystem II (PSII)]. Target-site resistance (TSR) mutations (Pro-197-Gln, Pro-197-Thr, and Trp-574-Leu) and non-target-site resistance (NTSR) mediated by cytochrome P450 monooxygenase (CYP450) genes were associated with pyroxsulam resistance. Pinoxaden resistance was conferred by two TSR mutations, which referred to a rare Ile-2041-Val mutation and a common Ile-1781-Leu mutation but with two different nucleotide substitutions (CTA/TTA). CYP450- and glutathione-S-transferase (GST)-mediated resistances were the main resistance mechanisms for this multiple herbicide-resistant (MHR) population to the PSII inhibitor isoproturon. This is the first case of a singleL. multiflorumpopulation evolving multiple resistance to three herbicide MoAs (ALS, ACCase and PSII) in China. Diverse resistance mechanisms including TSR and NTSR meanL. multiflorumexhibits a high degree of resistance plasticity.Keywords:Lolium multiflorumL.;pyroxsulam;pinoxaden;isoproturon;multiple herbicide resistance;TSR;CYP450;GST
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhiqin Ding,Mengdie Cai,Weimin Gan,Ping Yuan,Lijun Wei,Xianglei Cheng
In this paper, a novel, selective and sensitive high performance liquid chromatography-UV photolysis-fluorescence detection (HPLC-UV-FLD) method for nitrosamines (NAs) is proposed based on terbium-doped carbon dots (Tb-CDs). Experimental studies have shown that nitrosamines can be degraded by UV photolysis with wavelength at 254 nm to produce NO 2 − , while NO 2 − can rapidly and specifically quench the fluorescence of Tb-CDs. Based on this principle, N -nitrosodimethylamine (NDMA), Nnitrosomorpholine (NMor), N -nitrosopyrrolidine (NPYR), N -nitrosodiethylamine (NDEA) and N -nitrosopiperidine (NPIP) are separated within 8 min by HPLC. These five kinds of NAs showed a good linear relationship in the range of 10–2000 μg/L, and the limit of detection was as low as 2.25 μg/L. The proposed method is successfully applied to the detection of sweet potato, lotus root, potato, water chestnut, tomato and cucumber. The sample recoveries range from 77.35 % to 119.3 %.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Haiyan Wang,Jing Zhang,Yong Zhang
This study investigates the effects of mono-substituents (i.e., hydroxyl, methyl, amino and nitro groups) on the polarity-sensitive fluorescent probe properties of pyrene (Pyr) using fluorescence, UV–vis, and circular dichroism absorption spectroscopy. The results indicate that the four mono-substituents altered the fluorescence spectral characteristics of Pyr to varying degrees, with the most to least influential order being: nitro, amino, hydroxyl, and methyl. 1-Aminopyrene (1-APyr) and 1-nitropyrene (1-NPyr) are deemed unsuitable for use as polarity-sensitive fluorescent probes due to their limited or absent spectral characteristics. The I 386 /I 406 and I 376 /I 396 ratios of 1-HPyr and 1-MPyr decrease as solvents polarity increases, contrasting with the I 372 /I 384 ratio of Pyr. Thus, 1-hydroxypyrene (1-HPyr) and 1-methylpyrene (1-MPyr) also exhibit polarity-sensitive characteristics similar to Pyr, and their solubility in PBS buffer surpasses that of Pyr at 298 K. Moreover, 1-HPyr and 1-MPyr are practicable for detecting the polarity of human serum albumin (HSA) under simulated physiological conditions. The results underscore the potential of the polarity-sensitive mono-substituents of Pyr in biological applications, particularly in probing protein polarity and conformational changes, and further providing a potential tool for the related research in biochemistry and pathology.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Zhenjie Zheng,Jingxuan Wang,Mengqi Ma,Yunpeng Xu,Da Huang,Jian Wang,Cuiying Lin,Zhenyu Lin,Fang Luo
Sensitive and selective detection of N -nitrosodimethylamine (NDMA), a highly carcinogenic environmental contaminant, is essential for public health and safety. In this study, we have developed a homogeneous electrochemiluminescence (ECL) biosensor for ultrasensitive NDMA detection by integrating Nafion-functionalized covalent organic framework (COF) with aptamer recognition and magnetic separation. The imine-linked COF (COF-LZU1) scaffold not only enables stable encapsulation of Ru(bpy) 3 2 + , but also creates a confined microporous environment that locally concentrates c (TPrA) and enhances the generation and survival of TPrA radicals, thereby amplifying the ECL signal. Nafion further improves Ru loading and ionic conductivity. Biotinylated aptamers were initially immobilized on streptavidin-coated magnetic beads (MBs) and hybridized with complementary DNA-modified Ru@COF-LZU1 to form Ru@COF-LZU1-MBs complex. Upon NDMA binding, a conformational switch releases Ru@COF-LZU1 into the solution. After magnetic separation, the ECL signal of the supernatant is measured in the presence of TPrA. This homogeneous format avoids electrode surface modification, which is commonly required in conventional Ru@COF-LZU1-based ECL sensors, offering improved reproducibility and simplified operation. The biosensor exhibits a wide linear range (100 fg/mL - 100 ng/mL) with a low detection limit of 9.11 fg/mL. This platform offers a sensitive, reproducible, and facile strategy for monitoring NDMA and similar small-molecule contaminants in complex matrices.
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