BMC Veterinary Research
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2026
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IF 3.1
Yao Lulu,Kang Yunzhe,Zhao Xuyang,Wang Lucai,Lv Lijie,Zhu Wenhui,Yang Xiuwen,Zhuang Guoqing,Sun Aijun
Background N6-methyladenosine, the most prevalent post-transcriptional modification in eukaryotes, plays a critical role in regulating gene expression and disease pathogenesis. Concurrently, G-quadruplex structures are implicated in key biological processes, including the regulation of transcription and post-transcriptional events. Emerging evidence suggests that m 6 A modifications and G4 structures colocalize within viral genomes and human pre-mRNAs; however, their functional interplay remains poorly understood. Results Using existing MeRIP-seq data from Marek’s disease virus (MDV)-infected samples, we investigated the colocalization pattern and its potential role in viral infection. Our bioinformatic analyses revealed that the predicted G4 structures were predominantly two G-tetrad G-quadruplexes. Genes with m 6 A-G4 colocalization were significantly enriched in pathways related to immune response and tumorigenesis. Then we verified the presence of co-localization on the CCL4 , which inhibited CCL4 expression. Co-localization also affected viral replication and regulated ICP4 expression. Conclusions We systematically characterized the features of G4 structures co-localized with m 6 A modifications during MDV infection, investigated the role in viral replication and gene regulation, and provided experimental evidence for m 6 A-G4 co-localization. These findings shed new light on the epitranscriptomic regulatory mechanisms employed by an oncogenic virus and may inform the development of novel antiviral and anti-tumor therapeutic strategies.
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
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2025
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IF 4.7
Shuaishuai Cui,Zhaoyang Jin,Xuyang Ma,Dao Lin,Xiangjun Li,Yujian He,Li Wu
The G-quadruplex (G4) DNA structures are a noncanonical nucleic acid secondary structure involved in numerous fundamental biological processes, including replication and transcription. Accordingly, regulating the folding/unfolding of G-quadruplexes in genes is important for understanding the studies of G4-associated biological processes. Here, we propose a reduction-sensitive G4 binder for regulation of replication and transcription of G4-containing DNA, thereby enabling specific control over G4-associated biological processes. Biophysical studies, combined with molecular dynamics simulations, displays the reduction-sensitive azobenzene ligands target binding to G4 structure in template DNA, while dissociate the G4 structure by the reductive cleavage of the ligand. In vitro polymerase assays revealed that azobenzene ligands stalled the replication and transcription of template DNA at G4, and the inhibitory effects were significantly weakened on polymerase processivity with incubation of ligands at high concentration of GSH. Finally, we integrated the G4-forming sequence and G4-negative control into a synthetic reporter gene, respectively. We observed effective suppression in expression of G4-containing gene by adding the ligands compared to the corresponding G4-negative control in a way, and effective restoration of gene expression induced by their reductive states of ligands. Our findings highlight the potential of endogenous-responsive G4 binders in advancing specific regulation of biological process in a G4-dependent manner, but may also represent a promising strategy as a future therapeutic modality.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
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IF 9.221
Hualin Guo,Pengfei Ma,Ke Li,Shuxia Zhang,Yin Zhang,Huiqing Guo,Zhouping Wang
Ochratoxin A (OTA) is known as an important mycotoxin that is widely distributed in food products. The increasing requirement for sensitive and convenient OTA detection has promoted the development of aptasensors based on nanomaterials and elegant design strategy for aptamer probes. Ratiometric fluorescence aptasensors greatly reduce the probability of generating false-positive signals, compared to one-signal detection methods. In this work, an efficient ratiometric aptasensor was constructed, based on the dual-emission fluorescence of N -methyl mesoporphyrin IX (NMM) and copper nanoclusters (CuNCs). The key binding region of aptamer was obtained by theoretical results of molecular docking, and experiments of optimizing the length of complementary DNA (cDNA) verified the conjecture. With the addition of OTA, CuNCs-cDNA dissociated from the aptamer sequence, and the fluorescence of CuNCs (λ em =510 nm) decreased. Meanwhile, the aptamer contained G-quadruplex structure and enhanced the fluorescence of NMM (λ em =610 nm). In this way, fluorescence intensity at the emission wavelength of 610 nm and 510 nm were monitored and I 610 /I 510 was set as the signal output of this aptasensor. Stability tests indicated that the established aptasensor can reduce the interference of pH and storage time. The limit of detection (LOD) was as low as 0.045 ng/mL, with a wide linear range of 0.05–100 ng/mL. Moreover, specificity tests provided excellent selectivity towards other common mycotoxins, and the applicability was confirmed by performing real sample analysis, indicating that the NMM/CuNCs ratiometric biosensor for OTA detection has practical application prospects in food samples.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
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IF 8
Xin Gao,Yun Yin,Junyu Xie,Shaohua Gong,Xuemei Li
CRISPR/Cas13a system has been combined with different isothermal amplification strategies for RNA analysis. Nevertheless, expensive enzymes are usually indispensable in these isothermal amplification methods, which increases the detection cost. In this study, a CRISPR/Cas13a-mediated triple signal amplification strategy has been designed for selective, sensitive and inexpensive detection of viral RNA. SARS-CoV-2 S RNA activated CRISPR/Cas13a to cut hairpin DNA, triggering catalytic hairpin assembly (CHA) and subsequent hybridization chain reaction (HCR) to form a long-stranded Y-type DNA. G-quadruplexes were distributed on the branch of the long-stranded DNA and bound with N-methyl mesoporphyrin IX (NMM) to enhance the fluorescence signal. SARS-CoV-2 S RNA was specifically detected with a limit of detection (LOD) of 285 fM. Notably, no additional expensive enzymes were required in the entire detection process. The CRISPR/Cas13a-mediated triple signal amplification strategy holds great potential for specific and sensitive viral RNA detection with low cost.
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高影响因子
危险品化学品经营许可证（带存储）