Artificial Cells Nanomedicine and Biotechnology
·
2014
·
IF 0
Xiulan Su,Song Guo,Xiaoxia Huang,Xiang Wang,Donglai Qi,Chengmin Yang
Oxidative reactions of hemoglobin (Hb) are still a serious problem for Hb-based blood substitute development. Although varieties of antioxidant strategies have been suggested, this in vitro study examined the ability of the ascorbate, N-Acetyl-L-Cysteine (NAC), 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine-1-oxygen free radicals (TEMPO) and their reductant system in preventing Hb oxidation. The content of ferric Hb is monitored in the process of vitamin C (Vc), NAC, TEMPO and their reductant system. The results suggest that ascorbate is effective in reducing ferryl Hb, and TEMPO with Vc/NAC could obviously shorten the reaction time, but it does not play the role of Met-Hb reductases. It demonstrates that TEMPO did little to recover Hb under oxidative stress.
研究领域:
生物化学
·
医学工程
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DOI
PubMed
JOURNAL OF PLANT BIOLOGY
·
2024
·
IF 2.2
Zhan Niheng,Zhao Ziyi,Ren Lingyan,Yang Fuxin,Zhang Chuhan,Qin Lijun,Gong Xian
NAC (NAM/ATAF1,2/CUC2) is one of the most important plant-specific transcription factors, playing a significant role in plant growth, morphogenesis, and stress resilience. In this investigation, 77 members of the NAC gene family were identified from the Eucommia ulmoides database, and these E. ulmoides NAC families were classified into 18 subfamilies based on their protein sequences. Currently, the architectural design, conserved domains, and cis -acting elements of EuNAC genes, as well as physicochemical properties of EuNAC proteins, have also been analyzed. In addition, the expression patterns of selected EuNAC genes under five heavy metal (Cu 2+ , Cd 2+ , Pb 2+ , Mn 2+ , and As 5+ ) imposed stresses were investigated. The results showed that the expression of EuNAC9 experienced a significant induction to upregulate under these five heavy metal stresses, suggesting that EuNAC9 may mediate the response to these five heavy metal stresses. In addition, the function of EuNAC9 was investigated in both transgenic yeasts and tobaccos. The transgenic yeasts exhibited enhanced resistance to Mn 2+ stress by increasing the expression of ScSMF1 and ScSOD2 , whereas the transgenic tobacco grew better under Mn 2+ stress and showed intense antioxidant enzyme activity to alleviate the accumulation of H 2 O 2 and O 2 − . Moreover, subcellular localization indicated EuNAC9 was a nuclear localization protein, and the transcriptional activity domain of EuNAC9 resided at the C-terminal. This study will provide a theoretical framework for analyzing the molecular regulatory mechanisms of plant responses to heavy metals mediated by EuNAC9 genes and elucidating the biological roles of E. ulmoides NACs in responding to heavy metal stress.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Yuanjing Zhu,Fangfang Song,Yanyun Ju,Liyuan Huang,Lu Zhang,Chuliang Tang,Hongye Yang,Cui Huang
Purpose In this study, we aimed to develop a unique N-acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.Materials and methods We first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in N, N-dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).Results NAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/(NAC@MSN) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/(NAC@MSN) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.Conclusion A PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/(NAC@MSN) for bone tissue engineering.
研究领域:
组织工程
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材料科学
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细胞生物学
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再生医学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2024
·
IF 5.5
Wali Muhammad,Min Liang,Beiduo Wang,Jieqi Xie,Wajiha Ahmed,Changyou Gao
N-Acetyl cysteine (NAC) is an essential molecule that boosts acute lung injury (ALI) defense via its direct antioxidant capability. Nevertheless, the therapeutic use of NAC is limited due to its poor bioavailability and short half-life. In this study, NAC was grafted to the polyurethane consisting of poly(propylene fumarate), poly(thioketal), and 1,6-hexamethylene diisocyanate (PFTU) to reduce excessive oxidative stress and inflammatory factors in ALI. The NAC-grafted polymer nanoparticles (NPT@NPs) were prepared as a drug delivery system, which could effectively scavenge free radicals and reduce inflammation in vitro. The administration of NPT@NPs exhibited notable efficacy in ameliorating pulmonary edema, attenuating the presence of inflammatory cells, suppressing myeloperoxidase expression, diminishing the levels of pro-inflammatory cytokines, and reversing cell apoptosis in an ALI model induced by lipopolysaccharide (LPS). The NPT@NPs demonstrated significantly better efficacy compared to the free NAC in mitigating the deleterious effects of LPS on pulmonary tissue, thereby providing more effective protection against pulmonary inflammation.
研究领域:
药理学
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纳米技术
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肺科学
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炎症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2024
·
IF 4.1
Gang Luo,Wen Yang,Zijian Geng,Yiyi Cheng,Yingqing Xu,Yimeng Xiao,Jiying Liu
The Pichia pastoris expression system is a favorable platform for production of pharmaceutical proteins. Treatment of strains with N-acetyl-L-cysteine (NAC) has been shown to enhance the yield of recombinant proteins, thereby contributing to a reduction in production costs. However, the specific mechanism of action of NAC remains unclear. Previous research has indicated that glutathione (GSH) and autophagy are involved in the increased production of human serum albumin and porcine follicle-stimulating hormone β (HSA-pFSHβ) by NAC. This study investigated the potential interaction between GSH and autophagy in the production of HSA-pFSHβ. The findings indicated that sulfhydryl-free antioxidants such as melatonin, vitamin C, or vitamin E did not exhibit similar effects to NAC in enhancing HSA-pFSHβ yield. Moreover, NAC was found to enhance HSA-pFSHβ production by modulating GSH metabolism to reduce GSH consumption, increase total GSH levels, as well as glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities. Additionally, inhibition of autophagy through disruption of autophagy scaffolding proteins Atg1 or Atg11 led to an increase in recombinant HSA-pFSHβ production. Furthermore, NAC significantly decreased the phosphorylation of Slt2, and the absence of the SLT2 gene influenced the effect of NAC on HSA-pFSHβ secretion by modulating mitophagy and GSH metabolism. In conclusion, these results suggest a complex interplay between GSH metabolism and autophagy in the regulation of NAC-induced HSA-pFSHβ secretion.
研究领域:
分子生物学
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生物技术
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药用蛋白质
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酵母表达系统
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DOI
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Zhiquan Qiang,Zhen Zeng,Danfeng Ma,Jinzhe Li,Yuang Zhao,Tao Qin
Drought serves as a major environmental stress that restricts both the yield and quality of perennial ryegrass. Therefore, it is important to identify the essential genes that determine drought tolerance in perennial ryegrasses. In this paper, we isolated a drought-induced NAC transcription factor LpNAC22 . Transcriptional activity assays in yeast and plant cells indicated that LpNAC22 has transcriptional activation function. Subcellular localization observations revealed that LpNAC22 localized in the nucleus, compatible with its function as a transcription factor. LpNAC22 overexpression plants had enhanced drought tolerance and reduced cell membrane damage, whereas the knockdown of LpNAC22 in perennial ryegrass reduced plant drought tolerance and led to aggravated cell membrane damage. Late embryogenesis abundant (LEA), well-known stress resistance proteins, can protect the cell membrane from damage during drought conditions. DNA affinity purification sequencing and transcriptional regulation analysis demonstrated that LpNAC22 upregulates two LEA family genes, LpLEA1 and LpLEA2-1 , by directly binding to their promoters. Furthermore, we demonstrated that overexpression of LpLEA1 and LpLEA2-1 in Arabidopsis enhanced drought tolerance and reduced cell membrane damage under drought conditions. Our findings provide evidence that LpNAC22 improves drought resistance by modulating the transcription of LEA family genes in perennial ryegrass.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2024
·
IF 2.6
Chen Wanqiu,Chen Ting,Fang Min,Zhu Weiju,Li Cun
In this work, a simple and sensitive N-acetyl-L-cysteine (NAC)-covered CdTe quantum dots (NAC-CdTe QDs) fluorescence probe for continuous detection of Co 2+ and pyrophosphate ions (PPi, P 2 O 7 4− ) was synthesized. The fluorescence of the quantum dots was significantly quenched by Co 2+ through the coordination of Co 2+ and the carboxyl groups on the NAC-CdTe quantum dots. Interestingly, the combination of NAC-CdTe quantum dots with Co 2+ yields a new fluorescence probe of Co 2+ -modified NAC-CdTe QDs (Co 2+ @NAC-CdTe). The addition of PPi restored the fluorescence due to the competition between PPi and carboxyl groups with Co 2+ causing Co 2+ to detach from the surface of Co 2+ @NAC-CdTe quantum dots. Thus, a sensitive and reversible detection of Co 2+ and PPi had been successfully established. The Co 2+ @NAC-CdTe quantum dots fluorescence probe exhibits excellent selectivity and high sensitivity toward PPi detection with low detection limit of 0.28 μM. In addition, the novel fluorescence probe was successfully applied to detect the concentration of PPi in environmental water samples and in-vitro cells imaging.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1632
·
IF 4.8
Khan Hanif,Zeb Umar,Haider Imran,Rao Weiyi,Wang Chun,Guan Junjie,Alanzi Abdullah R.,Herqash Rashed N.,Abbasov Mehraj A.,Xing Shihai
Extreme environmental conditions have significantly affected the natural habitats of Dendrobium huoshanense , a valuable medicinal orchid highly sensitive to abiotic stress. NAC transcription factors are key plants regulators, contributing to growth, development, and responses to environmental stresses. Despite their importance, information on the NAC gene family in D. huoshanense remains limited. In this study, a genome-wide analysis was conducted to identify and characterize D. huoshanense NAC genes, using integrated bioinformatics, phylogenetic, and expression profiles. A total of 77 DhNAC genes were identified, unevenly distributed across 19 chromosomes, with chromosome 4 containing the highest number of 16 genes. Phylogenetic and structural analysis grouped the DhNAC genes into 20 groups, with conserved NAM domains and diverse exon–intron structures, indicating both evolutionary conservation and functional specialization. Promoter region analysis identified the presence of diverse stress-responsive cis-regulatory elements, such as drought, cold, abscisic acid, methyl jasmonate, and salicylic acid signaling pathways, indicating their roles in stress adaptation. Comparative genomic studies with Arabidopsis thaliana , Dendrobium nobile , Oryza sativa , and Zea mays highlighted evolutionary relationships and revealed significant gene duplication, including tandem and segmental events. Finally, expression analysis of selected DhNAC gene using qRT-PCR showed differential expression under cold stress, methyl jasmonate, abscisic acid, and salicylic acid treatment. Under stress treatments, DhNAC genes showed distinct temporal expression patterns, with ABA inducing early, intermediate, and late responders, MeJA driving strong early-to-mid responses, and SA promoting sustained late responses. Key genes including DhNAC9 , DhNAC19 , DhNAC48 , DhNAC51 , DhNAC53 , DhNAC55 , DhNAC58 , and DhNAC73 were consistently responsive, highlighting their central roles in stress adaptation. These findings provide valuable insights into the role of NAC genes in D. huoshanense in response to environmental stresses.
研究领域:
植物学
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分子生物学
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遗传学
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植物逆境生理学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Tao Feng,Bin Pi,Bin Li,Lei Jiang,Yi-Meng Wang,Xue-Song Zhu,Hui-Lin Yang
Calcium phosphate cements (CPCs) are popular bone filling materials and drug carriers. However poor mechanical properties and lack of osteoinduction restrict their clinical applications. Recent studies suggested the osteogenic properties of NAC. In our study, we incorporated NAC with α-TCP/SF. We found that the compressive strength of α-TCP/SF-NAC composites increased with increase in NAC concentration, possibly due to complex three-dimensional networks of SF induced by NAC, which was large and chemically heterogeneous and induced compact oriented growth of HA crystals. However the setting time increased slightly with the addition of NAC, due to the ruptured disulfide bonds in SF. The α-TCP/SF-NAC composites also showed decent biocompatibility in vitro. As a result, these composites hold great potential as bone filling materials for clinical applications, including minimally invasive surgeries.
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DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2021
·
IF 5.194
Yilong Cui,Miao Song,Bonan Xiao,Menglin Liu,Pengli Liu,Yanfei Han,Bing Shao,Yanfei Li
The problem of excessive aluminum (Al) content in food is widespread. After Al enters the body, it can cause mineral metabolism imbalance and reactive oxygen species (ROS) overproduction, which ultimately leads to bone impairment. ROS is mainly produced in mitochondria and acts on mitochondria. Mitochondrial damage is closely related to mitophagy and apoptosis . In order to clarify whether ROS-mediated mitophagy and apoptosis are involved in Al-induced femoral impairment, forty-eight male C57BL/6 N mice were exposed to AlCl 3 (179.3 mg/kg) and/or NAC (100 mg/kg) for 90 days. Our results showed that NAC inhibited the mitophagy and apoptosis, and alleviated growth inhibition, mineral metabolism imbalance, structural damage, decreased bone mineral density and decreased bone formation factor expressions in the femora of Al-treated mice. These results suggest that ROS-mediated mitophagy and apoptosis are involved in Al-induced femoral impairment in mice, exogenous ROS clearance is a potential strategy for the treatment of Al-induced bone impairment.
研究领域:
毒理学
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骨骼生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2022
·
IF 6.1
Yi Li,Wenyan She,Tangxi Guo,Tianhe Huang,Yixin liu,Pan Liu,Xiaoran Xu,Xinyu Wang,Miao Wang,Chaochao Yu,Yi Liu,Yongchang Wei
Developing novel drugs for gastric cancer (GC) is greatly needed, and a reactive oxygen species (ROS)-modulating strategy has been demonstrated to be useful for cancer treatment. However, no organic arsenical-derived ROS-modulating drug has been developed in GC. Here, we constructed ACZ2 and investigated its efficacy and potential mechanism for GC in vitro and in vivo. Our data showed that ACZ2 could inhibit GC proliferation and cause G2/M phase arrest. Moreover, ACZ2 induced ROS accumulation by depleting glutathione (GSH) and TrxR1, triggering a subsequent ER stress response by activating the PERK/EIF2/ATF4/CHOP signalling pathways, which is a crucial step for ACZ2-mediated apoptosis and autophagy. Vitally, ROS scavenger (NAC) and ER stress inhibitor (4PBA) reversed ACZ2/ROS/ER stress-mediated apoptosis and autophagy. Our in vivo results clearly demonstrated that ACZ2 suppressed tumour growth in a GC xenograft model. Collectively, our data indicated that ACZ2 is a potential agent against GC.
研究领域:
肿瘤学
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药理学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 21
·11833-11841
·
IF 3.049
Kangquan Zhao,Bin Pi,Liping Zhao,Shoujin Tian,Jianfei Ge,Huilin Yang,Weiping Sha,Liming Wang
The properties of polymethyl methacrylate (PMMA) bone cement make it a popular bone filling material. However, its disadvantages, such as lack of biodegradability and osteogenesis, restrict its clinical application. Studies have indicated the osteogenic properties of N-acetyl cysteine (NAC) and the biodegradability of 2-methylene-1,3-dioxepane/methyl methacrylate-based (MDO/MMA) copolymers. In this study, we developed bioactive PMMA cements through modification with fixed concentrations of NAC and different proportions of MDO. The purpose of this study was to compare the mechanical properties, morphology, NAC release, biocompatibility, degradability and mineralization capability of modified bone cements with those of conventional cement. The specific-modified specimens (NAC-p (5% MDO-co-MMA)) exhibited a lower bending modulus but had little effect on compressive strength. This material was morphologically compact and nonporous, similar to conventional PMMA bone cement. NAC could be released from NAC-p (5% MDO-co-MMA) continuously and appropriately. NAC-p (5% MDO-co-MMA) was biologically safe and showed satisfactory tissue compatibility. Ester was introduced into the polymer, which reinforced the degradation properties of NAC-p (5% MDO-co-MMA). NAC-p (5% MDO-co-MMA) enhanced the mineralization capability of osteoblastic cells.
研究领域:
材料科学
·
生物材料
·
骨科工程
·
生物医学工程
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DOI
PubMed
高影响因子
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