CURRENT MICROBIOLOGY
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2019
·Vol. 76
·期 6
·706-712
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IF 1.595
Li Minghua,Meng Xiumei,Sun Zhiyang,Zhu Chunjie,Ji Huiying
It is well known that cofactors play a key role in the production of different compounds in bioconversion processes, while the high cost of cofactors limits their usage in industrial applications. In the present study, a NADH regeneration system was successfully developed in Lactobacillus plantarum by expressing the fdh gene coding for formate dehydrogenase (FDH) from Candida boidinii. Results indicated that the FDH was expressed with the highest activity of 0.82 U/mg of protein when cells entered early stationary phase. In addition, the expression of FDH increased the intracellular level of NADH and NADH/NAD + ratio in L. plantarum , and therefore, enhanced the NADH-dependent production of 3-phenyllactic acid (PLA) in repeated and fed-batch bioconversions. In brief, the results demonstrate that the NADH regeneration by expressing FDH is a promising strategy for producing NADH-dependent microbial metabolites in L. plantarum .
研究领域:
微生物学
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生物技术
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代谢工程
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2021
·
IF 5.097
Liqiang Yang,Kunpeng Yin,Yang Zhong,Hao Zheng,Lingzhi Zhao,Lian-Wen Qi,Juanjuan Peng
Mitochondrial damage is implicated with a variety of pathological processes and is featured by the dramatically raised NADH level. To date, the detection and in situ imaging of NADH in living animals is still a challenge. Herein, we designed an upconversion luminescent nanoprobe for the in vivo imaging of NADH. The Nile blue (NB) with a strong absorption band at 500–700 nm can quench the red luminescence of the upconversion nanoparticle at 654 nm by the fluorescence resonance energy transfer effect. Under the presence of NADH, the NB was bleached, so that the fluorescence of the probe was “turned on”. The nanoprobe achieved highly selective detection of NADH, and the in vivo monitoring of NADH was also demonstrated on living mice.
研究领域:
生物化学
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医学影像学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
·
IF 3.232
Fengmao Li,Yunming Tang
Peroxidase , a key enzyme causing enzymatic browning, and affected the potential values of fruit and vegetables. Phytic acid and NADH inhibited peroxidase in a competitive manner due to their reducing properties, and it's IC 50 (1.18 ± 0.32) × 10 −8 , (8.02 ± 0.45) × 10 −6 mol L −1 , respectively. The interaction between phytic acid, NADH and peroxidase contributed to intrinsic fluorescence quenching and conformation alternation with a accuracy determination by multispectroscopic techniques (fluorescence spectra, FT-IR and CD spectra), respectively. Molecular docking simulation revealed that phytic acid, NADH interacted with His170, Ala34, Arg38, Ser73, Arg31, Lys174, Gln176, Asn175, Arg75; Gln176, Asn175, Phe221, Lys174, Gly173, Ser167, Phe172, Gly169, His170 in peroxidase, respectively and blocked substrates into catalytic reactions.
研究领域:
食品科学
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生物化学
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植物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Fei-Long Li,Qiang Zhou,Wei Wei,Jian Gao,Ye-Wang Zhang
Enzymatic NADP + regeneration is a promising approach to produce valuable chemicals under economic conditions. Among all the enzymatic routes, using water-forming NADH oxidase is an ideal one because there is no by-product. However, most NADH oxidases have a low specific activity to NADPH. In this work, a thermostable NADH oxidase from Lactobacillus rhamnosus (LrNox) was rationally engineered to switch its specificity from NADH to NADPH. The results show that mutants D177A, G178R, D177A/G178R, D177A/G178R/L179S improved the NADPH activity by a factor of 4–6. The highest NADPH catalytic efficiency ( K cat /K m 223.71 S −1 μm −1 , 47.6-fold higher than wild-type LrNox) and 51% of NADH activity retention were achieved by replacing the single amino acid Leu179 for serine (L179S) in LrNox. Modeling of L179S-NADPH complex reveals that the phosphate group of NADPH interacts with the hydroxyl of Ser179 with a strong hydrogen bond and several shorter hydrogen bonds with the amino group of Lys185 could stabilize the binding of NADPH in the L179S mutant. This work provides an efficient method for converting NAD(P)H specificity and shows that L179S mutant is a potential and efficient auxiliary enzyme for NADP + regeneration.
研究领域:
生物化学
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生物技术
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酶学
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DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
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2024
·
IF 4.4
Xuyong Tang,Fengxi Li,Sijun Huang,Li Xu,Lei Wang,Yi Guo
Nicotinamide-adenine dinucleotide (NADH) as biomimetic units combined with copper ion to form laccases mimics (Cu-NADH). Cu-NADH has the enhanced activity of copper center to mediate aryl C–H amination using air as green oxidants. It could successfully catalyze oxidative coupling between different aromatic compounds and 4-phenylurazole (yield: 72 ∼ 91 %). Recycling catalytic ability (up to 6 times) showed its excellent stability. The Cu-NADH nanozyme was certificated to catalyze C–H amination effectively, and highlighted that it was a proper way to design catalysts according to enzyme-like catalyzing non-natural reaction.
研究领域:
生物化学
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催化化学
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环境科学
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DOI
高影响因子
ELECTROANALYSIS
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2012
·Vol. 24
·期 6
·1334-1339
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IF 2.872
Yang Fan,Xin Yang,Chunpeng Yang,Jinhang Liu
We describe a simple method for preparing Au-TiO 2 /graphene (GR) nanocomposite by deposition of Au nanoparticles (NPs) on TiO 2 /GR substrates. The as-prepared Au-TiO 2 /GR was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The presence of Au NPs on TiO 2 /GR surface remarkably improves the electrocatalytic activity towards the oxidation of hydrogen peroxide (H 2 O 2 ) and β -nicotinamide adenine dinucleotide (NADH). The Au-TiO 2 /GR modified glassy carbon (GC) electrode exhibits good amperometric response to H 2 O 2 and NADH, with linear range from 10 to 200 µM and 10 to 240 µM, and detection limit of 0.7 and 0.2 µM, respectively.
研究领域:
生物传感器
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电化学分析
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DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2020
·
IF 2.857
Simin Cao,Haoyang Li,Yangyi Liu,Mengjie Zhang,Mengyu Wang,Zhongneng Zhou,Jinquan Chen,Sanjun Zhang,Jianhua Xu,Jay R. Knutson
The ultrafast solvation dynamics of reduced nicotinamide adenine dinucleotide (NADH) free in solution has been investigated, using both a femtosecond upconversion spectrophotofluorometer and a picosecond time-correlated single-photon counting (TCSPC) apparatus. The familiar time constant of solvent relaxation originating in “bulk water” was found to be ∼1.4 ps, revealing ultrafast solvent reorientation upon excitation. We also found a slower spectral relaxation process with an apparent time of 27 ps, suggesting there could either be dissociable “biological water” hydration sites on the surface of NADH or internal dielectric rearrangements of the flexible solvated molecule on that timescale. In contrast, the femtosecond fluorescence anisotropy measurement revealed that rotational diffusion happened on two different timescales (3.6 ps (local) and 141 ps (tumbling)); thus, any dielectric rearrangement scenario for the 27 ps relaxation must occur without significant chromophore oscillator rotation. The coexistence of quasi-static self quenching (QSSQ) with the slower relaxation is also discussed.
研究领域:
生物物理学
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化学动力学
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DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2025
·
IF 3.7
Xinyu Chen,Jiahui Xie,Hui Wang,Yujie Tang,Yezhou Yang,Pilang Zheng,Qihua Yang,Xin Liu,Xiaobo Li
Graphical A novel composite material integrating β-ketoenamine-linked nano TPTTA COFs and a rhodium complex electron mediator into a mesoporous silica matrix is reported for photocatalytic NADH regeneration. This design enhances electron transfer, achieving a 75.7% NADH yield, 89.8% selectivity, and an initial rate of 8.7 g NADH •g Cat −1 •h −1 , with stable performance over four cycles. The photocatalytic regeneration of NADH represents an effective means of mimicking photosynthesis, but confronts the challenges of the charge separation, electron transfer, and the selectivity control in the surface reduction stage. Herein, we report a multifunctional composite material that embeds β-ketoenamine-linked nano TPTTA covalent organic frameworks (COFs) and electron mediator (rhodium complex, [Cp*Rh(bpy) (H 2 O)Cl 2 ] into a functional mesoporous silica matrix for photocatalytic NADH regeneration. This strategy enables the facilitation of the electron transfer process while maintaining the intrinsic properties of the COFs, providing flexibility in COFs design. The strategic integration of electron mediator and the shortened charge migration path due to the nanosized COFs results in an NADH regeneration yield of 75.7% and a selectivity of 89.8% for 1,4-NADH. The initial reaction rate for NADH regeneration attained 8.7 g NADH •g Cat −1 •h −1 , and the system showed no decrease in activity after four reaction cycles.
研究领域:
生物化学
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材料科学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2022
·
IF 10.383
Xiewen Wu,Song Wang,Jing Fang,Hui Chen,Hongbo Liu,Run Li
Inspired by natural photosynthesis, photocatalytic NADH regeneration has drawn increasing interest in the recent decade as it provides a perfect approach for NAD+ reduction into NADH, which can be further consumed by oxidordeuctase for enzymatic redox reactions. However, two issues still remain unsolved in this procedure. First, the photocatalytic efficiency in NAD+ hydrogenation requires further improvement. Second, the rhodium electron mediator [Cp*Rh(bpy)H2O]2+ (M), which is always required for selective 1,4-NADH regeneration, is difficult to recover because of its good solubility in aqueous solution. Given the high price of M, it is highly wasteful and inefficient if it only spends once. Here, we report a Cp*Rh(bpy)Cl implanted conjugated microporous polymer DTS/Rh@CMPs which can be employed as a highly effective visible light photocatalysts for in situ NADH regeneration without using additional M. In addition, the insertion of Rh complex into a polymer skeleton, as demonstrated in UV–vis, fluorescence, photocurrent and electrochemical impedance, dramatically improves the light absorption capacity and the electron separation and transfer efficiency. Compared with that of DTS@CMP-1 with M, an enhanced reaction yield of 33% was determined in DTS/Rh@CMP-1 suggesting that intramolecular electron transfer has a better activity than that of intermolecular electron transfer in photocatalytic NAD+ reduction. Moreover, as the Rh complex is rooted firmly in a polymer framework, negligible Rh loss and conversion decrease in NADH regeneration are observed. When the DTS/Rh@CMP-1 was coupled with yeast alcohol dehydrogenase (YADH, from Saccharomyces cerevisiae), 1.36 mM of methanol was accumulated, implying an excellent biocompatibility of DTS/Rh@CMP-1 and a high feasibility of photobiocatalysis for formaldehyde hydrogenation.
研究领域:
生物化学
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材料科学
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酶工程
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DOI
PubMed
高影响因子
Cell Discovery
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2021
·Vol. 7
·期 1
·1-12
·
IF 10.849
Meng Wensi,Zhang Lijie,Cao Menghao,Zhang Yongjia,Zhang Yipeng,Li Ping,Kang Zhaoqi,Guo Shiting,Xu Ping,Ma Cuiqing,Gao Chao
Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions.
研究领域:
微生物学
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代谢工程
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Guojun Weng,Xinju Zhao,Jing Zhao,Jianjun Li,Jian Zhu,Junwu Zhao
The plasmonic spectral sensing method based on the etching of gold nanorods has been a powerful tool in the field of biomedical detection. Among the plasmonic etching sensors, the most popular sensing strategy is the “positive oxidative etching” plasmonic sensor that is based on plasmon blue-shifting due to nanorod shortening. In this study, we proposed a nanoplasmonic detection of dihydronicotinamide adenine dinucleotide (NADH) based on negative oxidative etching: NADH-mediated inhibition of H 2 O 2 induced gold nanorods etching. In the presence of NADH, the bromine intermediates (Br 3 − and Br 2 ) used for etching gold nanorods were reduced and consumed, inhibiting the etching rate and resulting in a small blue-shift of the longitudinal plasmon band (LPB). This shift can be utilized to sense the presence of NADH. Under the optimal reaction conditions, the LPB difference (Δλ) of etched gold nanorods and the concentration of NADH had a good linear relationship in the range of 0–4.9 × 10 −6 mol/L. The nanoplasmonic sensing showed a satisfactory preference for NADH over other common substances tested. It was successfully applied to the detection of NADH in PBS solution. This system provides a platform for the detection of targets in NADH-generated enzyme catalysis.
研究领域:
生物医学检测
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纳米技术
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分析化学
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DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2022
·Vol. 28
·期 55
·e202201430
·
IF 5.02
Yuanyuan Zhang,Jian Liu
Graphical An artificial photosystem was constructed through the covalent attachment of Rh complex onto PCN. The integrated photocatalyst could regenerate NADH efficiently by integrating the light harvesting unit and the electron transfer unit through conjugated connection. By further coupling with FDH immobilized on the hydrophobic membrane, an enhanced HCOOH production could be obtained. Natural photosynthesis is a highly unified biocatalytic system, which coupled cofactor (NAD(P)H) regeneration and enzymatic CO 2 reduction efficiently for solar energy conversion. Mimicking nature, a novel system with Rh complex covalently grafted onto NH 2 -functionalized polymeric carbon nitride (NH 2 -PCN) was constructed. The integrated connection of the light-harvesting and electron mediation modules as Rh m3 -N-PCN could promote the efficient NAD + reduction to NADH. As a result, the integrated system exhibited a conversion of ∼66 % within 20 minutes. By further coupling in situ generated NADH with formate dehydrogenase (FDH), a photoenzymatic production of formic acid (HCOOH) from CO 2 was accomplished. Moreover, by immobilizing FDH onto a hydrophobic membrane, an enhanced HCOOH production of ∼5.0 mM can be obtained due to the concentrated CO 2 on the gas-liquid-solid three-phase interface. Our work herein provides an integrated strategy for coupling the anchored electron mediator with immobilized enzyme for enhanced artificial photosynthesis.
研究领域:
生物化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
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