Thioredoxin reductase (TrxR) is a pivotal biomarker and therapeutic target, monitoring its expression levels with good accuracy and sensitivity in cells or tissues is of great significance for clinical disease diagnosis and prognosis studies. Herin, we first develop a novel TrxR fluorescence sensing strategy based on the nucleic acid site-specific cleavage (NASC) mechanism, which highlights the benefits of prominent selectivity and high sensitivity. Notably, our NASC-based sensing strategy shows the limit of detection (LOD) is over two orders of magnitude lower than other site-specific cleavage-based approaches. Our proposed method has demonstrated the successful application in determining the TrxR level of in living cell and tissue, highlighting its potential for analyzing TrxR in clinical samples. The results convincingly illustrated that the NASC strategy stands as a highly promising molecular instrument for unraveling the physiological roles of TrxR within living organisms.
Portable near-infrared (NIR) lighting source stimulates great efforts to exploit NIR phosphor-converted LEDs (pc-LEDs) that push the rapid development of broadband NIR-emitting phosphors. Although Cr 3+ -activated NIR phosphors designed by multisites occupation strategy show significant potential in emission bandwidth, poor spectral stability caused by different thermal quenching behaviors is a brake on practical application due to different local structure around Cr centers. One-site-based NIR phosphors seem to be an effective method to solve aforementioned problem. Herein, we report a type of new ultra-broadband one-site-based NIR-emitting phosphors NaSc 1-x P 2 O 7 :xCr 3+ (NSP:xCr 3+ ) with emission at 910 nm and full width at half maximum (FWHM) of up to ∼200 nm. Their FWHM value exceeds all one-site-based Cr 3+ -activated phosphors and some multiple-sites ones. Structural analysis and low-temperature spectra demonstrate that such impressive broadband NIR emission originates from Cr 3+ occupying at single octahedral Sc site. At 425 K, integrated emission intensity of NSP:0.04Cr 3+ phosphor maintains 53.8% of room temperature. A NIR pc-LED prototype is fabricated by using NSP:0.04Cr 3+ phosphor with a blue LED chip, and its multipurpose applications in non-destructive detection, night vision and analysis of foodstuff are demonstrated. These findings indicate that broadband NSP:0.04Cr 3+ NIR phosphor enabled by one-site occupation strategy has potential prospect for multipurpose NIR pc-LEDs applications.
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