Ming Ying,Qin Li,Jingbo Wu,Yihang Jiang,Zhourui Xu,Mingze Ma,Gaixia Xu
Breast cancer is characterized by the uncontrolled proliferation of breast epithelial cells under the action of a variety of carcinogens. Although HER2-inhibitors were currently applied for HER2-positive breast cancer patients, they didn’t work for patients with resistance to HER2-targeted anti-cancer drugs. In this work, we prepared novel CuS@BSA-NB2 nanoparticles (NPs) for breast cancer photothermal therapy (PTT). The NPs had good biocompatibility due to the Bovine Serum Albumin (BSA) encapsulating and excellent targeting to HER2 because of nanobody 2 (NB2). Under 808 nm laser irradiation, CuS@BSA-NB2 NPs had high photothermal conversion efficiency and photothermal stability. Meanwhile, we constructed a stable cell line of MDA-MB-231/HER2 with a high expression of HER2 protein. Immunofluorescence and ICP-MS assays showed that CuS@BSA-NB2 NPs can be specifically enriched and be ingested in MDA-MB-231/HER2 cells. Furthermore, CuS@BSA-NB2 NPs had shown a more significant photothermal treatment effect than CuS@BSA under certain treatment conditions for MDA-MB-231/HER2. In addition, the cytotoxicity assay demonstrated that CuS@BSA-NB2 NPs had a low toxicity for MDA-MB-231/HER2 cells. The above results suggested that CuS@BSA-NB2 NPs were great photothermal therapeutic agents to reduce the malignant proliferation of breast epithelial cells and have potential for breast cancer therapy.Keywords: CuS@BSA-NB2 nanoparticles, photothermal therapy, HER2, NB2, MDA-MB-231/HER2
Yang Zaizhi,Shen Yalong,Zhu Yuan,Jiang Xiaoming,Liu Rui,Yang Zhen,Wang Shouliang,Xu Dong
Flash sintering has revolutionized the field of advanced ceramic preparation with the low heating temperature and extremely short sintering time. The difference of doped ions radius can influence the defect structure and resistivity of the sample through lattice distortion, subsequently altering the parameters and dielectric properties of flash sintering samples. By flash sintering technology, co-doped TiO 2 ceramics with the acceptor ions (Mg 2+ , Ca 2+ , Sr 2+ , Ba 2+ ) and donor ions (Nb 5+ ) were successfully prepared at 1100 °C for 24 min. According to the blackbody radiation model, Mg 2+ and Nb 5+ co-doped TiO 2 ceramics exhibit the highest surface temperature at the moment of flash sintering, approximately 1852 °C. Additionally, the co-doped TiO 2 ceramics with Ba 2+ and Nb 5+ ions exhibit the strongest polarization effect, with a dielectric constant above 10 5 . The acceptor ions with larger ions increase oxygen vacancies by enhancing the stability of defect complexes. The excellent giant dielectric properties are attributed to the combined action of the electron-pinned defect dipole effect and the internal barrier layer capacitor effect.
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