JOURNAL OF LUMINESCENCE
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2022
·
IF 4.171
Jing Ying Yang,Wen Ying Gao,Jian Hua Wang,Zhen Ming Dong,Yu Wang,Shao Min Shuang
Herein we present a new colorimetric and fluorescent probe , AH, derived from the Schiff base condensation between 4-(2-aminophenylimino)-7-nitrobenzofurazan and 2-hydroxy-1-naphthaldehyde for optical sensing of Cu 2+ ions. In aqueous CH 3 CN solution (CH 3 CN/H 2 O, 1:1, v/v , pH7.4), AH effectively complexed with Cu 2+ at a stoichiometric ratio of 1:1, producing nearly 25-fold fluorescence enhancement at 540 nm along with a significant color change from orange to grey blue. The sensing system exhibits high specificity for the detection of Cu 2+ . The detection limit is as low as 42.2 nM. The observed switching on fluorescence emission can be attributed to the suppression of photo-induced electron transfer (PET) as well as chelation enhanced fluorescence (CHEF) effect after coordination of AH with Cu 2+ ion via the unshared electron pair on the phenyl amine. A test strip with AH has been fabricated for identifying Cu 2+ . Fluorescence imaging study reveals that probe AH is capable to sense Cu 2+ in living HeLa cells, Malpighian tubules of locust and zebrafish.
研究领域:
分析化学
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生物医学
·
光学传感
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产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 18
·7625-7630
·
IF 1.821
Yi Li,Jinhui Zhou,Xiaofeng Xue,Liming Wu,Lanzhen Chen,Jinzhen Zhang,Shuming Yang
An optimization method of NBD-F derivatization has been developed for the determination of proline in honey using high performance liquid chromatography (HPLC) with fluorescence detection. To determine the free proline in different samples of honey, ultrasound extraction was performed using 0.1 M borate buffer solution as the extraction solvent. Chromatographic separation was performed on a XDB C18 column (150 × 4.6 mm i.d.) using 0.1 M sodium acetate buffer solution (pH 7.2) : methanol : tetrahydrofuran (900 : 95 : 5, respectively, v/v/v) as mobile phase A and methanol as mobile phase B at a flow rate of 1.0 mL min−1 with a run time of 10 min. The assay was linear over a range of 0.15 μg mL−1 to 100.00 μg mL−1 with a lower limit of detection of 3.00 mg kg−1, and recoveries were greater than 90%. The intra-day and inter-day precision was less than 4%. This method was successful in determining free proline in honey, ranging from 80.5 mg kg−1 to 426.4 mg kg−1 in different honey samples.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
TALANTA
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2021
·
IF 6.057
Dongmei Wang,Jiajia He,Junyong Sun
The thiolysis of 7-nitro-1,2,3-benzoxadiazole amine (NBD-A) paves the way for specific sensing of H 2 S over biothiols and real-time imaging in living organisms. Rational fabrication of NBD-A-based probe with ratiometric mode and two-photon excitation is highly appealing to achieve high quality bioimaging. In this work, the NBD-A molecules are assembled with poly(9,9-dioctylfluorenyl-2,7-diyl) polymer nanoparticles , defined as NBD@PFO, to construct two-photon ratiometric probes for H 2 S detection through the fluorescence resonance energy transfer (FRET). For the construction of NBD@PFO nanohybrids , polymer nanoparticles are employed as the NBD-A molecular vehicle, energy donor and two-photon absorber, while NBD-A is served as the response unit and energy acceptor. Taking advantages of NBD-A and polymer nanoparticles, the obtained NBD@PFO probes exhibit high selectivity, fast response (<5 s), ratiometric detection and two-photon excitation. Our results indicate that NBD@PFO nanohybrids are successfully applied for monitoring of H 2 S concentration in living cells and zebrafish, exhibiting great potential of polymer nanoparticles to improve the imaging capability of an organic small molecular probe .
研究领域:
生物化学
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分子生物学
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纳米医学
·
生物成像
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DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Jun Xu,Ying Wang,Menghua Huang,Xiaojuan Xu,Yueyun Zeng,Xuan Luo,Shicheng Pei,Keming Xu,Wenying Zhong
Compared to conventionally synthesized organic compounds, peptides with amphiphiles have unique advantages, especially in self-assembly. Herein, we reported a peptide-based molecule rationally designed for the visual detection of copper ions (Cu 2+ ) in multiple modes. The peptide exhibited excellent stability, high luminescence efficiency, and environmentally responsive molecular self-assembly in water. In the presence of Cu 2+ , the peptide undergoes an ionic coordination interaction and a coordination-driven self-assembly process that leads to the quenching of fluorescence and the formation of aggregates. Therefore, the concentration of Cu 2+ can be determined by the residual fluorescence intensity and the color difference between peptide and competing chromogenic agents before and after Cu 2+ incorporation. More importantly, this variation in fluorescence and color can be presented visually, thus allowing qualitative and quantitative analysis of Cu 2+ based on the naked eye and smartphones. Overall, our study not only extends the application of self-assembling peptides but also provides a universal method for dual-mode visual detection of Cu 2+ , which would significantly promote point-of-care testing (POCT) of metal ions in pharmaceuticals, food, and drinking water .
研究领域:
分析化学
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材料科学
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环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
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2020
·Vol. 12
·期 12
·1617-1623
·
IF 2.596
Guoqiang Liu,Hongwei Ge,Ranhao Yin,Long Yu,Congming Sun,Weiru Dong,Zhenli Sun,Khalid A. Alamry,Hadi M. Marwani,Suhua Wang
To discriminate hydrogen sulfide (H2S) from thiol nucleophiles with similar reactivity is always a major challenge in developing new detection methods. In this paper, we report a colorimetric and ratiometric fluorescence probe selectively reactive to H2S, which was synthesized by hybridizing the fluorescent nitro-benz-2-oxa-1,3-diazole (NBD)-based derivative P-NBD with carbon dots (CDs). The selective reaction can be monitored by distinct ratiometric fluorescence or colorimetric variation. The nanohybrid probe exhibited two fluorescence bands centered at 440 nm and 543 nm, respectively. The yellow fluorescence at 543 nm from P-NBD is quenched by H2S, while the blue fluorescence is enhanced by weakening of the Förster resonance energy transfer (FRET) mechanism. It was observed that the apparent fluorescence color evolved from yellow to blue when exposed to different amounts of H2S, accompanied by the color change of the probe solution from yellow to red under natural light. The detection limit was estimated to be 57 nM in aqueous solution. We successfully applied this probe for the detection of H2S in real water samples, suggesting its potential application in environmental monitoring and protection.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
TETRAHEDRON
·
2021
·
IF 2.457
Peixin Niu,Jiaojiao Liu,Yifan Rong,Xingjiang Liu,Liuhe Wei
This work proposes a fluorescent probe that uses coumarin as a chromophore and NBD as a sensing unit to detect hydrogen sulfide . The probe can work well in aqueous media, and can quickly respond to hydrogen sulfide. When detecting hydrogen sulfide, the probe has high selectivity and good anti-interference ability. Besides, in monitoring hydrogen sulfide in living cells and zebrafish, good results have demonstrated the probe’s potential in practical applications.
研究领域:
生物化学
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医学检测
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分子生物学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2017
·Vol. 41
·期 11
·4416-4423
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IF 3.269
Hui Yu,Yan Liu,Jiamin Wang,Qing Liang,Hong Liu,Jian Xu,Shijun Shao
A novel organic–inorganic ratiometric fluorescent probe AuNCs–NBD was developed for the detection of cysteine (Cys) and homocysteine (Hcy). In our design, the AuNCs–NBD exhibited dual-emission fluorescence properties with a single excitation. Bovine serum albumin (BSA) protected gold nanoclusters (AuNCs) with carboxyl groups were employed as a reference signal unit and conjugated with the p-aminothiophenol substituted NBD (7-nitro-2,1,3-benzoxadiazole) moiety to form the ratiometric fluorescent probe. The AuNCs–NBD composite showed a single red fluorescence at 630 nm ascribed to AuNCs, and reaction of AuNCs–NBD with Cys and Hcy caused the cleavage of the thioether bond and therefore generated the fluorescent amino linked NBD with emission at 540 nm. Accordingly, the green fluorescence of released NBD at 540 nm gradually increased upon increasing the concentration of Cys or Hcy, while the red fluorescence ascribed to AuNCs at 630 nm remained constant, which resulted in the ratiometric determination of Cys and Hcy using the AuNCs–NBD probe. Due to the specific recognition of NBD with Cys and Hcy, the AuNCs–NBD probe exhibited high selectivity for Cys and Hcy over other amino acids and GSH. In addition, the AuNCs–NBD composite displayed desirable properties such as high specificity, appreciable solubility, ratiometric fluorescence response and favorable detection limit. Moreover, the AuNCs–NBD probe has been successfully applied for monitoring and imaging of intracellular Cys and Hcy in HeLa cells.
研究领域:
分析化学
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生物医学
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细胞生物学
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DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
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2019
·Vol. 17
·期 4
·939-944
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IF 3.49
Leixia Mei,Suyun He,Li Zhang,Keming Xu,Wenying Zhong
We report the synthesis and self-assembly of fluorescent peptide amphiphiles (NBD-PA) composed of a fluorescent NBD probe and a peptide derivative VVAADD with a C12-alkyl-chain as the linker (NBD-C12-VVAADD). The self-assembly of NBD-PA formed beta-sheet structures at neutral pH in aqueous solution, contributed to an ∼10-fold increase in the fluorescence and quantum yield of NBD molecules, and conferred a supramolecular hydrogel with excellent viscoelastic properties, while gel-to-sol transition of NBD-PA occurred rapidly when the pH value was adjusted to strongly alkaline (e.g. pH 11). Through the pH-responsive self-assembly behavior, we further explored the relationship between fluorescence of NBD-PA and pH values. Interestingly, the fluorescence of the NBD-PA system exhibited an excellent sigmoidal function relationship (R2 = 0.9999) with the alkaline pH values, which enabled accurate pH measurement regardless of salt types and ionic strength of solvents. Furthermore, the fluorescence of NBD-PA was fully reversible upon cycles of pH shifts, with the chemical structure of NBD-PA well-maintained throughout the process. These features of NBD-PA would facilitate the design of in situ pH detection systems as well as pH-responsive actuators for various applications in future.
研究领域:
生物化学
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材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
·
IF 4.097
Shaofei Zhang,Hui Yang,Ying Ma,Yu Fang
A bis-nitrobenzoxadiazole (NBD) derivative of calix[4]arene (L 1 ) and a control compound (L 2 ), mono-NBD derivative of the calixarene were designed and prepared via click chemistry. Fluorescence studies demonstrated that L 1 as created can function as a switch with the presence of Ag + in THF, of which the free state of L 1 emits at ∼527 nm, but the binding state at 576 nm. Based upon this discovery, L 1 was studied as a chemo-sensor of Ag + in a mixture solvent of THF and H 2 O with a detection limit (DL) of ∼6.2 × 10 −7 mol/L. Presence of other commonly found metal ions shows little effect upon the determination. Moreover, the L 1 in the Ag + -L 1 complex could be fully released with introduction of HCHO, a bases for the sensitive and selective detection of the toxic chemical. The DL of this test is 6.6 × 10 −7 mol/L. Interestingly, binding and releasing of the fluorescent ligand could be repeated for at least 5 times. Furthermore, both sensing could be performed in a visualized manner. It is believed that the fluorescent compound as created should have a potential to find real-life applications.
研究领域:
化学
·
分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaojie Ren,Huihui Tian,Liu Yang,Long He,Yani Geng,Xingjiang Liu,Xiangzhi Song
Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are of great importance in many physiological and pathological processes. Due to their similar structure and reactivity of these three biothiols, it is a great challenge to simultaneously distinguish one from another. By virtue of two different fluorescence signals, nitrobenzofurazan ( NBD ) has been proved to be an effective sensing functional group as well as a green fluorophore precursor to construct fluorescent probes for simultaneous discrimination of Cys/Hcy and GSH. However, most of the reported NBD-based fluorescent probes suffered from a poor water-solubility, a slow response, double-wavelength excitation or two interfering fluorescence signals (narrow wavelength separation in λ max Em and/or big difference in Φ Fl ). In this study, we developed a fluorescent probe, MCQ-O-NBD , for simultaneous discrimination of Cys/Hcy and GSH using two matched fluorescent signals (well separated red and green fluorescence and comparable Φ Fl ), which could function in a pure aqueous media with a rapid response under a single-wavelength excitation. This probe exhibited high sensitivity and good selectivity as well as low detection limit, and had been successfully applied to visualize and differentiate Cys/Hcy and GSH in living cells.
研究领域:
生物化学
·
医学检测
·
荧光成像
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 18
·6848-6855
·
IF 3.069
Xiaoting Huang,Haiyang Liu,Jiewen Zhang,Boren Xiao,Fengxu Wu,Yueying Zhang,Ying Tan,Yuyang Jiang
A dicyanoisophorone-based near-infrared fluorescent probe (NIR-NP) was successfully designed for hydrogen sulfide (H2S) detection. 7-Nitro-1,2,3-benzoxadiazole (NBD) amine, an electron-withdrawing group, was applied to quench the fluorescence of dicyanoisophorone-based fluorescent dyes and could be removed by H2S-specific thiolysis of an NBD amine bond, leading to a significant fluorescence turn-on response. The probe could quantitatively detect H2S with a lower detection limit (0.03 μM) in vitro and in living cells. The probe with a large Stokes shift (186 nm) showed a high selectivity to H2S in the presence of other biothiols, including glutathione, homocysteine, and cysteine. Moreover, the probe was successfully applied to image endogenous H2S in different tumor cells and in liver tissues. The excellent properties of the probe and its applications in cell and tissue imaging indicated its potential for further exploration and application of H2S in the pathophysiology of cancers and even in the diagnosis of H2S-related diseases.
研究领域:
分子影像学
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肿瘤学
·
化学生物学
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分子生物学
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生物分析化学
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转化医学
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Lijun Wang,Fang Wang,Qiang Zhou,Yanfei Wang,Haixiang Song,Haiyang Yang
In recent years, the use of fluorescent amphiphilic polymers as the bioimaging agents has achieved significant progress, and promoted the development of the early stage cancer diagnosis and treatment. In this study, a serials of novel nitrobenzoxadiazole (NBD) fluorophore-functionalized polyester-based amphiphilic polymers were designed and synthesized via a two-step process: a hydrophobic polyester backbone was synthesized first by ring-opening alternating copolymerization (ROAC) of phthalic anhydride (PA) and allyl glycidyl ether (AGE) monomers using NBD-OH fluorophore as the initiator and metal-free Lewis pairs as the catalyst; then hydrophilic modified by three different hydrophilic pendants, PEG-SH, mercaptoacetic acid and mono-6-thio-β-cyclodextrin, through thiol-ene click reaction. Notably, directly using fluorophore as the initiator to initiate the ROAC of cyclic anhydrides and epoxides has never been reported. The influence of the feed ratio, type of Lewis pair and reaction temperature upon the polymerization performance were presented and the quantitative introduction of NBD and three kinds of hydrophilic pendants were described in detail as well. Due to the amphiphilic properties, these polymers could facilely self-assemble into fluorescent polymeric nanoparticles, which were characterized by a series of characterization techniques including surface tensiometer, dynamic light scattering (DLS), transmission electronic microscopy (TEM), and fluorescence spectroscopy. These fluorescent polymeric nanoparticles with diameters of tens or hundreds of nanometers showed good particle stability and good fluorescence performance in aqueous solution. The biocompatibility of these fluorescent polymeric nanoparticles was also excellent. In fact, the biological imaging of A549 cells could be realized successfully in the presence of the fluorescent polymeric nanoparticles. This work provides a new strategy and may inspire new approaches to the design and preparation of the biocompatible fluorescent amphiphilic polymers which could be used as the potential biological imaging agents in basic life science research and clinical applications.
研究领域:
材料科学
·
生物医学工程
·
高分子化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）