Biomaterials Science
·
2018
·Vol. 6
·期 7
·1834-1845
·
IF 5.831
Jiong Li,Xuandong Wang,Dongye Zheng,Xinyi Lin,Zuwu Wei,Da Zhang,Zhuanfang Li,Yun Zhang,Ming Wu,Xiaolong Liu
Theranostic nanoprobes integrated with dual-modal imaging and therapeutic functions, such as photodynamic therapy (PDT), have exhibited significant potency in cancer treatments due to their high imaging accuracy and non-invasive advantages for cancer elimination. However, biocompatibility and highly efficient accumulation of these nanoprobes in tumor are still unsatisfactory for clinical application. In this study, a photosensitizer -loaded magnetic nanobead with surface further coated with a layer of cancer cell membrane (SSAP-Ce6@CCM) was designed to improve the biocompatibility and cellular uptake and ultimately achieve enhanced MR/NIR fluorescence imaging and PDT efficacy. Compared with similar nanobeads without CCM coating, SSAP-Ce6@CCM showed significantly enhanced cellular uptake, as evidenced by Prussian blue staining, confocal laser scanning microscopy (CLSM) and flow cytometric analysis. Consequently, SSAP-Ce6@CCM displayed a more distinct MR/NIR imaging ability and more obvious photo-cytotoxicity towards cancer cells under 670 nm laser irradiation. Furthermore, the enhanced PDT effect benefited from the surface coating of cancer cell membrane was demonstrated in SMMC-7721 tumor-bearing mice through tumor growth observation and tumor tissue pathological examination. Therefore, this CCM-disguised nanobead that integrated the abilities of MR/NIR fluorescence dual-modal imaging and photodynamic therapy might be a promising theranostic platform for tumor treatment.
研究领域:
生物医学工程
·
肿瘤学
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 11
·2544-2552
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IF 4.2
Shaojie Wang,Yongbo Wang,Yuanna Ning,Qiming Liu
Antibiotic residues pose a serious threat to ecosystems and food safety. Developing convenient, visual, and on-site detection methods is therefore in high demand and has a practical purpose. In this work, a near-infrared (NIR) fluorescent probe with an analysis platform based on a smartphone has been constructed for quantitative and on-site detection of metronidazole (MNZ). CdTe quantum dots with NIR emission at 710 nm (QD710) were prepared by using a simple hydrothermal method and showed good properties. A spectral overlap between absorption of MNZ and excitation of QD710 resulted in an effective inner filter effect (IFE) between QD710 and MNZ. Because of the IFE, the fluorescence of QD710 decreased gradually with increasing concentrations of MNZ. Based on the fluorescence response, quantitative detection and visualization of MNZ was achieved. NIR fluorescence analysis and the special IFE between probe and target can improve sensitivity and selectivity for MNZ. Additionally, these were also utilized for quantitative detection of MNZ in real food samples and the results were reliable and satisfactory. Meanwhile, a portable visual analysis platform in a smartphone was constructed for on-site analysis of MNZ, which can be used as an alternative method for detection of MNZ residues in situations with limited instrumental conditions. Therefore, this work provides a convenient, visual, and real-time analysis method for detection of MNZ and the analysis platform shows great potential for commercialization.
研究领域:
分析化学
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环境科学
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食品安全
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生物技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Juan Ou-Yang,Chun-Yan Li,Yong-Fei Li,Bin Yang,Song-Jiao Li
Alkaline phosphatase (ALP) is an essential enzyme in phosphate metabolism and widely distributes in mammalian body fluids and tissues. So far, various fluorescent probes for ALP have been well developed, but some disadvantages such as short emission wavelength and low photostability restrict their biological application. Herein, a near-infrared (NIR) fluorescent nanoprobe based on infinite coordination polymer nanoparticles (CyOH@Tb-GMP) is constructed for endogenous ALP activity assay. The characteristics of the nanoprobe are as following: (1) The fluorescence emission of the nanoprobe is at 718 nm belonging to NIR region, which is suitable for bioimaging. (2) The nanoprobe displays better photostability than common NIR dyes such as ICG as well as CyOH. (3) The nanoprobe exhibits high sensitivity for ALP activity with a 7.5-fold fluorescence enhancement when 2.5 U/mL ALP is added. (4) The NIR nanoprobe is employed for monitoring endogenous ALP activity in various biological samples such as cell, tissue and mice with satisfactory results.
研究领域:
生物化学
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分子生物学
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纳米技术
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医学影像
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Wei Jiang,Huiyuan Zhang,Jilian Wu,Guangxi Zhai,Zhonghao Li,Yuxia Luan,Sanjay Garg
Quercetin (QT) is one promising candidate for the treatment of various cancers with virtually no toxic side effects. However, its anticancer effect is severely restricted by its poor bioavailability, low water solubility, and chemical instability in the neutral and alkaline medium. Herein, zeolitic imidazolate framework-8 (ZIF-8) is first reported as the multifunctional nanoplatform to the codelivery of quercetin as an anticancer agent and CuS nanoparticles as a photothermal therapy (PTT) agent for synergistic combination of chemotherapy and PTT as well as overcoming the drawbacks of quercetin. Moreover, folic acid–bovine serum albumin (FA–BSA) conjugates are applied to stabilize the CuS@ZIF-8-QT to promote the bioavailability of quercetin and realize active-targeting drug delivery. Near-infrared (NIR) fluorescent imaging demonstrated the highly increased drug accumulations of FA–BSA/CuS@ZIF-8-QT in tumors, resulting from efficient internalization via FA-receptors-mediated endocytosis. The results of in vivo and in vitro anticancer experiments demonstrate that quercetin and PTT agent can work together efficiently under NIR irradiation, thus remarkably improving the anticancer effect. Therefore, our newly designed FA–BSA/CuS@ZIF-8-QT multifunctional drug delivery system might be a promising nanoplatform for cancer treatment.
研究领域:
癌症治疗
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纳米技术
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药物递送系统
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2016
·
IF 8.387
Dan Chen,Bowen Li,Songhua Cai,Peng Wang,Shuwen Peng,Yuanzhi Sheng,Yuanyuan He,Yueqing Gu,Haiyan Chen
Dual targeting towards both extracellular and intracellular receptors specific to tumor is a significant approach for cancer diagnosis and therapy. In the present study, a novel nano-platform (AuNC-cRGD-Apt) with dual targeting function was initially established by conjugating gold nanocluster (AuNC) with cyclic RGD (cRGD) that is specific to α v β 3 integrins over-expressed on the surface of tumor tissues and aptamer AS1411 (Apt) that is of high affinity to nucleolin over-expressed in the cytoplasm and nucleus of tumor cells. Then, AuNC-cRGD-Apt was further functionalized with near infrared (NIR) fluorescence dye (MPA), giving a NIR fluorescent dual-targeting probe AuNC-MPA-cRGD-Apt. AuNC-MPA-cRGD-Apt displays low cytotoxicity and favorable tumor-targeting capability at both in vitro and in vivo level, suggesting its clinical potential for tumor imaging. Additionally, Doxorubicin (DOX), a widely used clinical chemotherapeutic drug that kill cancer cells by intercalating DNA in cellular nucleus, was immobilized onto AuNC-cRGD-Apt forming a pro-drug, AuNC-DOX-cRGD-Apt. The enhanced tumor affinity, deep tumor penetration and improved anti-tumor activity of this pro-drug were demonstrated in different tumor cell lines, tumor spheroid and tumor-bearing mouse models. Results in this study suggest not only the prospect of non-toxic AuNC modified with two targeting ligands for tumor targeted imaging, but also confirm the promising future of dual targeting AuNC as a core for the design of prodrug in the field of cancer therapy.
研究领域:
纳米技术
·
癌症生物学
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药物科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 11
·2202817
·
IF 10
Tianguang Liu,Yanli Chen,Huizhe Wang,Mengyuan Cui,Jiaqi Zhang,Wancun Zhang,Peng Wang
Asymmetrical heptamethine cyanine with near-infrared (NIR) absorption is used for photothermal therapy (PTT) of cancer. Aiming to overcome the drawbacks caused by the high temperature of PTT, the development of asymmetrical heptamethine cyanine with photothermal and photodynamic properties is still an attractive strategy. Different from the traditional method of the heavy atom effect, in this work, the carboxyl or sulfonic groups are introduced into the indole ring or branch chain of asymmetrical heptamethine cyanine to afford a series of new phototherapy agents. After being encapsulated by DSPE-PEG 2000 , BSS-Et NPs exhibit robust photostability, efficient reactive oxygen species generation (49%), and excellent photothermal conversion efficiency of about 37.6% under 808 nm laser irradiation. BSS-Et NPs possess passive tumor-targeting properties in vivo to not only visualize the tumor by NIR fluorescence imaging but also eliminate the tumor without any recurrence by photodynamic therapy and PTT synergistic therapy under laser irradiation. In addition, benefitting from the characteristics of organic small molecules, they can be metabolized quickly through the liver without inducing toxicity in the whole body. In general, this study provides a new direction for the development of multifunctional phototherapy agents for cancer treatment.
研究领域:
癌症治疗
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药物化学
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Kai Wang,Wei Wang,Shi-Yu Chen,Jia-Cheng Guo,Jia-Heng Li,Yu-Shun Yang,Xiao-Ming Wang,Chen Xu,Hai-Liang Zhu
Sulfur dioxide (SO 2 ) is an important marker in the human body, and maintaining its balance is very significant for human health. The high concentration of SO 2 will cause harm to human health because of the transient nature of SO 2 . To measure the level of SO 2 , it is urgent to find a suitable detection method for the real-time monitoring of sulfite. Herein, we designed and exploited a Near-Infrared (NIR) turn-on fluorescent probe L derived from rhodamine for the detection of SO 3 2− in living cells and zebrafish. The addition of sulfite would attack the 4-position of the carbonyl group of levulinic acid, resulting in the cleavage of the ester bond and the formation of the fluorophore . The intramolecular charge transfer (ICT) was restored thus the monitoring system emitted the corresponding long NIR fluorescence at 646 nm. Probe L had high selectivity, a short response period within 10 min, and the detection limit of 0.6 μM. Besides, Probe L had a long excitation wavelength (>580 nm), which was in favor of more deeply biological imaging properties. Moreover, Probe L had low cell cytotoxicity through MTT assay, thus it was confirmed with good biocompatibility . In addition, probe L could successfully real-time monitor exogenous and endogenous sulfite in living cells, and be applied in zebrafish. The confocal results suggested that probe L had strong potential application prospect of outstanding capabilities for detecting sulfite in clinical diagnostics.
研究领域:
化学
·
生物化学
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生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Haoyu Chen,Minjuan Wang,Qiquan Yang,Jing Liu,Feng Liu,Xiaohua Zhu,Shu Huang,Peng Yin,Xingfeng Wang,Haitao Li,Youyu Zhang,Meiling Liu,Mingjie Wei,Shouzhuo Yao,Yang Liu
The integration of reactive oxygen species (ROS) related photodynamic therapy (PDT) with the strategy of reshaping the tumor microenvironment (TME) has emerged as a potential approach for nanodiagnostic and therapeutic interventions. However, the therapeutic efficacy based on ROS treatments may be hindered by intracellular antioxidants such as glutathione (GSH) and tumor hypoxia. To address these challenges, a nanoplatform based on GSH-responsive multifunctional porphyrinic metal–organic framework (PCN-224@Au@MnO 2 @HA, PAMH) was proposed. It was developed through a layer-by-layer in-situ growth method. This method avoids the need for high-temperature calcination and complex modification processes while improving the stability of PCN-224 in a phosphate-rich environment. GSH depletion leads to oxidation–reduction imbalance in TME. With the inactivation of GSH peroxidase 4 (GPX4), the content of hydrogen peroxide (H 2 O 2 ) increases, ultimately triggering lipid peroxidation (LPO) and promoting ferroptosis. The catalase-like activity of Au nanozymes facilitates the generation of oxygen (O 2 ), thereby mitigating tumor hypoxia and downregulating hypoxia-inducing factors (HIF-1α). Due to the presence of porphyrin ligands in PCN-224, the generated O 2 can be further converted to toxic singlet oxygen ( 1 O 2 ) under laser irradiation. Additionally, the platform allows near-infrared (NIR) fluorescence imaging, providing real-time information on intracellular GSH changes during PDT and ferroptosis. The PAMH nanoplatform has shown effective inhibition of tumor growth in subcutaneous models via both intravenous and intratumoral injection, indicating its potential in modulating reactive oxygen/sulfur species and reshaping TME, thereby facilitating imaging-guided cascaded nanocatalytic therapy.
研究领域:
纳米医学
·
癌症治疗
·
生物化学
·
材料科学
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Ziwei Zhao,Susu Yan,Yanan Li
Curcuminoid boron difluoride dyes have garnered significant attention in the realm of bioimaging, primarily due to their excellent photophysicochemical properties. However, the strong hydrophobicity limits their optimal near-infrared (NIR) fluorescence emission, and the synthesis process is complicated. In this study, Curc-TPA, a curcuminoid boron difluoride derivative with high fluorescence quantum yield, was simply synthesized and surprisingly exhibited aggregation-induced NIR emission in dimethyl sulfoxide/water mixture. The compound self-assembled into stable and homogeneous nanoparticles through hydrophobic interaction with amphiphilic polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol), which showed enhanced aggregation-induced emission (AIE) capacity in the NIR region. The nanoparticles specifically enriched at the tumor site due to enhanced penetration and retention effect and exhibited bright fluorescence imaging in vitro and in vivo. Notably, they showed satisfactory hematological compatibility and unobservable cellular and histological toxicity. Therefore, it is of promising clinical significance in the AIE-enhanced NIR fluorescence imaging-visualized precise diagnosis of cancer.
研究领域:
生物成像
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药物化学
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纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
·
2021
·
IF 8.947
Di Ma,Qingyu Zong,Yuchen Du,Fangzhou Yu,Xuan Xiao,Rong Sun,Yuan Guo,Xinhua Wei,Youyong Yuan
Subcellular organelle targeted imaging and therapy are of enormous interest in cancer theranostics. However, the lack of tumor-selective organelle targeting has compromised their efficacy and safety. In this work, we found that the near-infrared (NIR) fluorophore hemicyanine (CyNH 2 ) can selectively target mitochondria with strong cytotoxicity through decreasing the mitochondrial membrane potential and increasing the intracellular reactive oxygen species (ROS) levels. A macrotheranostic probe (denoted as PLCy) based on conjugating CyNH 2 with an acetylated lysine group was developed with masked fluorescence and cytotoxicity, which could both be unmasked through sequential activation by cancer cells overexpressing histone deacetylases (HDACs) and cathepsin L (CTSL) enzymes for selective cancer cell mitochondria-targeted imaging and therapy. In vitro and in vivo studies confirmed that the specific fluorescence turn-on and toxicity were restored in cancer cells and efficiently inhibited tumor growth. This macrotheranostic probe with sequential enzyme activation and mitochondrial targeting is expected to have promising applications in imaging-guided cancer therapy with high specificity and efficiency. Statement of significance To improve the targeting efficiency and enhance the anti-cancer activities of macrotheranostic probe. We designed macrotheranostic probe PLCy that can be activated via sequential enzymes for selective tumor mitochondria targeting. More importantly, the activated CyNH 2 can decrease the mitochondrial membrane potential and elevate the reactive oxygen species level in cancer cells without light irradiation, which can further induce apoptosis of tumor cells for chemotherapy. Therefore, the use of sequential enzyme activation and mitochondria targeting strategies in the context of enzymatic activation may provide a general strategy for organelle-targeted imaging and therapy with high specificity and efficiency.
研究领域:
癌症治疗诊断
·
分子成像
·
药物递送
·
生物化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）