FOOD CHEMISTRY
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2022
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IF 9.231
Yongxin Su,Zhenlin Li,Yinghui Zhao,Yijun Chen,Chen Luo,Xuri Wu
Extracts of Siraitia grosvenorii (Swingle), in Chinese known as Luo Han Guo (LHG), is authorized for use as a natural sweetener . LHG is rich in mogroside V that contains five glucoses, but also contains mogroside IIIE and analogues with fewer than three glucose units that cause an unpleasant aftertaste, limiting the use of the extract. Snailase was applied here to convert mogroside V in LHG extract in favor of siamenoside I formation, the sweetest mogroside with a taste similar to sucrose . For application, snailase was immobilized by adsorption to NKA (a macroporous resin), resulting in 10.9 U per g of adsorbed protein. Reuse of the NKA-adsorbed snailase was demonstrated for four cycles, and a continuous production of improved LHG extract at a 0.5 L scale had a productivity of 68.4 g/(L⋅day). The resulting product containing over 50% siamenoside I displayed an improved taste profile with satisfying safety toward HEK293T cells.
研究领域:
食品科学
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生物化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2021
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IF 7.514
Xiaomin Chen,Hui Wang,Xiaojun Huang,Shengkun Xia,Chunhua Chen,Qixing Nie,Shaoping Nie
This work established an effective method for kale flavonoids enrichment by resins. Resin screening, adsorption kinetics and isotherms, dynamic adsorption and desorption tests were conducted to optimize the appropriate resins and enrichment conditions. The results showed that NKA-9 was the optimum resin. The best adsorption conditions were 0.2 mg/mL flavonoids concentration, 12.5 bed volume (BV) sample volume and 2 BV/h adsorption rate. The desorption conditions were 3 BV of 80% ethanol at 2 BV/h elution rate. Under these conditions, the product purity was 31.16%. The purified flavonoids extract was mainly comprised of Kaempferol-3-O-sophoroside-7-O-diglucoside, Kaempferol-3,7,4′-O- d -triglucoside, Kaempferol-3-O-feruloyl-sophoroside-7-O- d -glucoside, and Kaempferol-3-O-sinapoyl-sophoroside. Moreover, it presented higher scavenging ability against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl ( OH) radical than crude extract. In conclusion, kale flavonoids can be well concentrated by NKA-9 resin and the purified flavonoids extract has good antioxidant activity which can be potentially applied in food, cosmetic or pharmacy industries.
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2018
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IF 6.735
Yuan Meng,Jianguo Jiang,Yuchen Gao,Aikelaimu Aihemaiti,Tongyao Ju,Yiwen Xu,Nuo Liu
Biogas upgrading technology is important for increasing the heating value of biogas via separating CH 4 and CO 2 , before biogas can be utilized as a vehicle fuel or for grid injection. In this work, using a porous polymeric resin (NKA-9), we prepared a new sorbent, namely polyethyleneimine (PEI)-impregnated NKA-9, for use in biogas upgrading to methane via CO 2 sorption firstly. The adsorption performance, regeneration behavior, cyclic stability and CH 4 /CO 2 separation performance were then evaluated in detail. The sorbent exhibited high adsorption capacity of up to 151.1 mg/g with negligible CH 4 adsorbed, demonstrating its high selectivity towards CO 2 over CH 4 . In column breakthrough experiments, binary mixtures were separated effectively under each set of reaction condition, where high CH 4 purities of >95% could be obtained as the final product in the outlet gas. As tested, excessive PEI loading, high temperature and low CO 2 concentration in biogas would result in unfavorable effects on biogas upgrading. In addition, we tested the regenerability of the sorbent using the two most common methods-N 2 and CO 2 purging, where the sorbent could be regenerated completely at temperatures above 85 °C and 155 °C, respectively. During the long-term operation, PEI-impregnated NKA-9 exhibited excellent cyclic stability, with 1.4% and 5.2% decreases in adsorption capacity after 30 adsorption-regeneration cycles. Detailed characterization revealed that the sorbent was chemically stable at 155 °C, in contrast to other conventional amine-modified sorbents. Therefore, the regenerable PEI-impregnated NKA-9 sorbent could be a promising choice for biogas upgrading to methane via CO 2 sorption.
研究领域:
环境科学
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化学工程
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能源技术
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产品关联
DOI
高影响因子
Journal of Oleo Science
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2022
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IF 1.628
Lihong He,Jiawei Zheng,Siting Feng,Li Xu,Nanjing Zhong
In this study, lipase A from Candida antarctica (CALA) was immobilized onto the macroporous resin NKA-9. Immobilization conditions (pH, time and CALA concentration) were studied, enzymatic activity and immobilization efficiency (IE) up to 968.89 U/g and 53.19% were respectively obtained under optimal conditions (immobilization pH 5.0, time 5 h and CALA concentration at 30 mg/mL). Then, the NKA-9 supported CALA (CALA@NKA-9) samples were used to catalyze glycerolysis in solvent-free system. With 0.25 g of the present CALA@NKA-9 (soybean oil 3.52 g and glycerol 0.184 g) and after 12 h reaction at 50 °C, diacylglycerols (DAG) content up to 64.37% and triacylglycerols (TAG) conversion at 83.33% were obtained. The relationship between temperature and TAG conversion was LnV 0 = 13.9310-6.4212/T for CALA@NKA-9. Meanwhile, the activation energy (Ea) of CALA@NKA-9 was calculated to be 53.39 kJ/mol. In addition, reusability in the glycerolysis reaction was also evaluated, and 57.82% of the initial glycerolysis activity was retained after 9 consecutive applications. Furthermore, the CALA@NKA-9 was also used to catalyze the esterification (esterification of fatty acids with glycerol), however, the present CALA@NKA-9 cannot initiate the esterification. Therefore, the present CALA@NKA-9 is shown to be potential for DAG production through glycerolysis reaction.
研究领域:
生物技术
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酶工程
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化学工程
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
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2025
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IF 8.2
Xinyu Yuan,Qiqi Feng,Qing Wang,Tao Wang,Zhu Qiao,Hongyu Pan,Yujie Zhong,Yongliang Zhuang
Walnut kernel pellicle, a by-product of the walnut industry, is rich in polyphenols. However, research on walnut kernel pellicle polyphenols (WPP) remains limited, and the composition and bioactivity of WPP are influenced by extraction methods, gastrointestinal digestion, and colonic fermentation. Therefore, this study aimed to optimize subcritical water extraction (SWE) and NKA-9 resin purification of WPP. Next, the changes in WPP throughout simulated gastrointestinal digestion and colonic fermentation were evaluated. The results demonstrated that SWE achieved higher polyphenol yield compared to ultrasound-assisted extraction, with ellagic acid and ellagitannins as the main components. Although digestion and fermentation reduced WPP contents and antioxidant activities, they facilitated the biotransformation of ellagic acid and ellagitannins into urolithins. Meanwhile, WPP modulated the gut microbiota by promoting beneficial bacteria. Additionally, WPP enhanced beneficial microbial metabolites, including short-chain fatty acids and indole derivatives. These findings highlighted the potential of WPP as a functional ingredient for gut health.
研究领域:
食品科学与技术
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营养生物化学
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植物化学
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肠道微生物学
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功能食品
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2022
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IF 7.425
Nan Sun,Jiayan Xie,Jianhua Xie,Yi Chen,Xiaobo Hu,Qiang Yu
In this study, the static adsorption–desorption performance of seven macroporous resins for bound polyphenols from mung bean coat dietary fiber (MBDF-BP) was compared, and NKA-9 macroporous resin was preferably screened for subsequent separation and purification. The composition of the purified products was identified and quantified, 44 major compounds were detected, with the main phenolic acid being p -hydroxybenzoic acid, which contained up to 8881.90 μg/g DW. The purification enriched flavonoids, with high contents of catechin (1419.03 μg/g DW) and vitexin (615.88 μg/g DW). The MBDF-BP purified products (pMBDF-BP) produced significant reversible inhibitory activity against α -glucosidase in a mixed-type inhibition manner, which was superior to the reported crude extracts. The antioxidant activity assays showed that pMBDF-BP exhibited distinct scavenging effects on DPPH•, ABTS + •, •OH free radicals, as well as reactive oxygen species (ROS) in Caenorhabditis elegans ( C. elegans ). These results demonstrated that NKA-9 macroporous resin could effectively enrich MBDF-BP extracts and enhance its antioxidant activity, which was promising to explore new sights into the applications of bound polyphenols from mung bean coat dietary fiber in functional foods or dietary supplements, thus contributing to the scientific utilization of mung bean coat resources and increasing the added value of related products.
研究领域:
食品科学
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植物化学
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生物化学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）