Analytical Methods
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2024
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IF 2.7
Zhiren Zhou,Yuting Tian,Lina Zou,Yanan Liu,Xueqing Zhang,Xiaojing Huang,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Nuclear matrix protein 22 (NMP22) is one of the most important tumor markers of bladder cancer and is significantly elevated in the urine of bladder cancer patients. Therefore, in this work, a highly sensitive ratiometric electrochemical immunosensor was constructed to detect NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs composites. ZIF-8 had a large surface area and good adsorption ability. Multi-Walled Carbon Nanotubes (MWCNTs) can optimize the electrical conductivity of ZIF-8, so that the electrode surface of ferrocene (Fc) obtains a stable and strong electrochemical signal. In addition, AuPt-MB provided another strong detection signal methylene blue (MB) while immobilizing the secondary antibody (Ab2) through Au–N and Pt–N bonds. A ratiometric electrochemical sensor was formed based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB, which showed a great linear connection between IMB/IFc and the logarithmic concentration of NMP22 with a detection limit of 3.33 fg mL−1 (S/N = 3) under optimized specifications in the concentration interval of 0.01 pg mL−1 to 1000 ng mL−1. In addition, the ratiometric immunosensor showed good selectivity and stability.
研究领域:
生物医学工程
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肿瘤学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Shengzhong Rong,Lina Zou,Ye Zhu,Ze Zhang,Hongfeng Liu,Yingcong Zhang,Hui Zhang,Hongmin Gao,Hongjun Guan,Jing Dong,Yupeng Guo,Fenghai Liu,Xiaoxia Li,Hongzhi Pan,Dong Chang
Nuclear matrix protein 22 (NMP22) is a crucial bladder cancer marker, the accurate detection of which is conductive to improving the effectiveness of tumor prevention and treatment. However, due to low sensitivity, it is impossible for conventional immunoassay to detect the trace of NMP22 at the early stages of tumor progression. It is crucial to develop a simple, sensitive and accurate detection method of NMP22. As a variety of two-dimensional (2D) material, reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) demonstrates excellent conductivity and contains abundant amino groups. As a sort of 3D material, silica-modified Cu-based metalorganic frameworks deposited silver nanoparticles ( [email protected] 2 @AgNPs) composites are characterized by large surface area, excellent biocompatibility and water stability, which allows the immobilization of anti-NMP22 in large amounts via Ag-N bonds. Through a combination of the advantages exhibited by 2D material, 3D material, AgNPs and disposable screen-printed electrode, a disposable and sensitive label-free electrochemical immunosensor has been successfully assembled for NMP22 detection. In addition to exhibiting a wide linear range (0.1 pg∙mL −1 -1000 ng∙mL −1 ) and a low detection limit (33.33 fg∙mL −1 , S/N = 3), the proposed immunosensor also demonstrated satisfactory selectivity, strong reproducibility and high stability. Moreover, the excellent performance in detecting human urine samples makes it applicable in practice.
研究领域:
生物医学工程
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分析化学
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癌症研究
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2018
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IF 3.235
Shuang Zhao,Yuchan Zhang,Songtao Ding,Jingchuan Fan,Zhi Luo,Kunguo Liu,Qi Shi,Weichi Liu,Guangchao Zang
A reliable and non-invasive electrochemical immunosensor for nuclear matrix protein 22 (NMP22) was presented based on reduced graphene oxide-tetraethylenepentamine (rGO-TEPA) and gold nanoparticles‑platinum nanoparticles-metal organic frameworks (AuNPs-PtNPs-MOFs) nanomaterial . rGO-TEPA is a promising template to enhance the loading ability of electrode for its large specific surface area and abundance in amino groups. AuNPs-PtNPs-MOFs nanomaterial was synthesized by NaBH 4 reduction method and applied to immobilize biomolecules due to their good biocompatibility, well conductivity , and excellent H 2 O 2 electrocatalytic ability. The electrochemical response signal was primarily originated from the obstruction of immobilized NMP22 antigen towards electrocatalytic reduction of H 2 O 2 and the signal was recorded by differential pulse voltammetry . Under optimum conditions, the immunosensor showed a sensitive response towards NMP22 at a broad concentration range from 0.005 ng·mL −1 to 20 ng·mL −1 . Two strong linear ranges could be observed at 0.005 ng·mL −1 to 0.5 ng·mL −1 (R 2 = 0.9833), and 0.5 ng·mL −1 to 20 ng·mL −1 (R 2 = 0.9909), with a low LOD of 1.7 pg·mL −1 (S/N = 3). Satisfactory reproducibility, stability and selectivity were obtained for NMP22 detection in real urine samples compared with standard ELISA assay, demonstrating the good analytical performance and potential non-invasive clinical application of this label-free electrochemical immunosensor.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2019
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IF 5.256
Ming Li,Lei Jiao,Siquan Liu,Lianhua Zhang,He Li
Ratiometric signal transducing strategies can improve the precision of immunoassay , which possess the unique merits of spatial-resolved signal readout and self-correcting towards possible false positive results. In this work, a new ratiometric electrochemical immunosensor has been developed for reliable detection of Nuclear matrix protein 22 (NMP22). Bioinspired synthetic melanin nanospheres (SMNPs) were elaborately chosen for the following considerations: 1) SMNPs can supply good biocompatible biorecognition interface for antibody anchoring; 2) SMNPs can chelate a large amount of lead ions (Pb 2+ ) and copper ions (Cu 2+ ) to fabricate two spatial-resolved electrochemical signals with different response manners towards NMP22. SMNPs chelated with Pb 2+ were used for the immobilization of captured primary anti -NMP22. And SMNPs chelated Cu 2+ were employed to prepare signal labels after anchored with anti -NMP22 antibody. After sandwich-type immunoreaction, with the increasing concentration of NMP22, the stripping peak current of Pb 2+ decreases while the stripping peak current of Cu 2+ increases. Thus, ratiometric electrochemical signals could be provided for NMP22 detection. The immunosensor presented a linear concentration range from 0.013 U mL −1 to 6.7 U mL −1 with a limit of detection of 0.005 U mL −1 towards NMP22. Meanwhile, satisfactory reproducibility, stability and selectivity of the immunosensor were demonstrated.
研究领域:
分析化学
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生物传感器
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电化学
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免疫分析
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
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2024
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IF 10
Jialuo Ai,Yixuan Huang,Zhaoyi Yin,Yingshan Deng,Ling Yan,Jingwen Liao,Guoyan Liang,Chong Chen,Yunbing Chang,Cairong Xiao,Jiale Zhou,Zurong Zhu,Chengli Liu,Zhuo Jiang,Chengyun Ning,Zhengao Wang
Combining the detection of tumor protein markers with the capture of circulating tumor cells (CTCs) represents an ultra-promising approach for early tumor detection. However, current methodologies have not yet achieved the necessary low detection limits and efficient capture. Here, we introduced a novel polypyrrole nanotentacles sensing platform featuring anemone-like structures capable of simultaneously detecting protein biomarkers and capturing CTCs. The incorporation of nanotentacles significantly enhanced the electrode surface area, providing abundant active sites for antibody binding. This enhancement allowed detecting nucleus matrix protein22 (NMP22) and bladder tumor antigen (BTA) with 2.39 and 3.12 pg/mL detection limit, respectively. Furthermore, our developed sensing platform effectively captured MCF-7 cells in blood samples with a detection limit of fewer than 10 cells/mL, attributed to the synergistic multivalent binding facilitated by the specific recognition antibodies and the positive charge on the nanotentacles surface. This sensing platform demonstrated excellent detection capabilities and outstanding capture efficiency, offering a simple, accurate, and efficient strategy for early tumor detection. This article is protected by copyright. All rights reserved
研究领域:
生物医学工程
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材料科学
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肿瘤学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）