RSC Advances
·
2023
·Vol. 13
·期 20
·13824-13833
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IF 3.9
Yani Zheng,Yin Dai,Junqiang Hong,Huizhu Fan,Qing Zhang,Wei Jiang,Wei Xu,Jianwen Fei,Junli Hong
The compound 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), one of the tobacco specific nitrosamines (TSNAs), is widely recognized as a major carcinogen found in tobacco products, environmental tobacco smoke and wastewater. Thus, a selective enrichment and sensitive detection method for monitoring the risk of NNK exposure is highly desirable. In this study, a magnetic molecularly imprinted polymer (MMIP) functionalized with dendritic nanoclusters was synthesized to selectively recognize NNK via the dummy template imprinting strategy, aiming to avoid residual template leakage and increase the imprinting efficiency. The nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, as well as vibrating sample magnetometry (VSM) and nitrogen adsorption/desorption analysis. The resulting MMIPs exhibited high adsorption capacity, fast binding kinetics and good selectivity for trace amounts of NNK. A rapid, low cost and efficient method for detecting NNK in tobacco products was established using magnetic dispersive solid-phase extraction coupled with HPLC-DAD with a good linear range of 0.1–250 μg mL−1. The limit of detection (LOD) and limit of quantification (LOQ) of NNK were 13.5 and 25.0 ng mL−1, respectively. The average recoveries were 87.8–97.3% with RSDs lower than 3%. The results confirmed that the MMIPs could be used as an excellent selective adsorbent for NNK, with potential applications in the pretreatment of tobacco products.
研究领域:
分析化学
·
环境科学
·
烟草科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Yu Tang,Zhe Wang,Menglin Li,Ruiping Zhang,Jinlan Zhang
A rapid, sensitive and wide coverage ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method has been developed and validated for the simultaneous quantitation of 14 alkylation DNA adducts in cell genomic DNA, RNA and cell contents isolated from the in vitro cultured human kidney cell line 293 T and the human liver cell line L02 exposed to 3 genotoxic reagents: N -methyl -N- nitrosourea (MNU), methyl methanesulfonate (MMS) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). After exposure, DNA was isolated and directly hydrolysed under acid conditions or digested by enzymes to obtain the hydrolysates containing DNA alkylation adducts followed by optimization of the pretreatment method and chromatographic separation conditions. Quantification was performed on a Waters ACQUITY UPLC BEH Amide column (1.7 μm, 2.1 × 150 mm) using an electrospray ionization (ESI) source in positive mode by selective reaction monitoring (SRM) at the precursor to product ion transitions of 14 analytes. The method showed selectivity, good linearity (r＞0.9950), accuracy (82.1%–115%), and intra-day (RSD%＜14%) and inter-day (RSD%＜15%) precision for 14 analytes. The recoveries of two pretreatment methods were all more than 50.5%, and no relative matrix effects were observed. Additionally, the samples were stable after short-term storage at 20 ℃ for 2 h, at 4 ℃ for 48 h or one cycle of freeze-thaw at −80 ℃. The established UHPLC-MS/MS method was used to evaluate the changes in alkylation DNA adducts and epigenetic modification-related methylcytosine after exposure to genotoxic reagents. For the first time, the results demonstrated that 3 genotoxic reagents induced different total amounts of adducts in the following sequence: MMS ＞ NNK ＞ MNU, and showed significant differences in the ratios of 7MeG to 1MeA and 1MeG to 1MeA in the 293 T cell model. Meanwhile, 293 T and L02 cells revealed significantly different DNA adduct formation characteristics in the contents of 1MeG and 1MeA. The DNA adduct formation relationships between DNA, RNA, and cell contents were probed to predict cancer risk and potential genotoxic exposure. This approach could be used to investigate the DNA adducts, their formation and the relationship to the mutagenicity or carcinogenicity of genotoxic reagents in future studies.
研究领域:
毒理学
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分析化学
·
分子生物学
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DOI
PubMed
高影响因子
GENE
·
2026
·
IF 2.7
Xia Xiao,Tingting Cui,Shengfei Hu,Shishi Shen,Yiying Huang,Chunping Cui,Hanzhang Tan,Wei Qiu,Yipeng Zhao
Multiple sclerosis (MS) is characterized by recurrent neuroinflammatory episodes that drive progressive neurodegeneration, with cigarette smoking recognized as a major environmental risk factor. Nicotine-derived nitrosamine ketone (NNK), a potent tobacco-specific nitrosamine, has been implicated in MS; however, its mechanistic contribution to disease pathogenesis remains poorly understood. Here, we applied an integrative multi-level approach to investigate the potential role of NNK in MS. Mendelian randomization (MR) analysis identified genetically predicted dipeptidyl peptidase-4 (DPP4) activity as being associated with MS susceptibility. Molecular docking further demonstrated feasible interactions between NNK and candidate proteins, including DPP4, providing theoretical plausibility for chemical–protein interactions. In experimental autoimmune encephalomyelitis (EAE) mice, systemic NNK exposure accelerated disease onset, aggravated neurological deficits, and enhanced immune cell infiltration and demyelination within the central nervous system, accompanied by increased DPP4 expression in inflamed regions. Consistent with these observations, in vitro NNK treatment impaired endothelial barrier integrity in bEnd.3 cells by reducing tight junction proteins (ZO-1, Claudin-5, and Occludin) and upregulating adhesion molecules (VCAM-1 and ICAM-1). NNK exposure also triggered inflammatory activation in BV2 microglia, resulting in elevated expression of TNF-α, IL-1β, and IL-6. Importantly, DPP4 silencing partially restored endothelial junctional integrity and attenuated pro-adhesive signaling in endothelial cells while reducing inflammatory cytokine expression in microglia, suggesting that DPP4 is functionally involved in NNK-induced neurovascular inflammation. Together, these findings suggest that NNK exposure may modulate neuroinflammatory processes relevant to MS through blood–brain barrier (BBB) disruption and microglial activation, with DPP4 potentially being involved in this process. This study provides mechanistic insights into how smoking-associated toxins may influence MS progression and highlights DPP4-related pathways as potential targets for therapeutic investigation.
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DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Wen Bo Zhu,Ling Gao,Yu Kang Zhang,Qi Zhang,Ying Hong,Wei Jian Shen,Ying Wang,Jian Hua Zhu
Liquid adsorption of TSNA by ZIF-8 was studied in this paper for the first time, applying the deformable molecular sieve to adsorb and separate TSNA. ZIF-8 can adsorb the bulky NNK molecules whose size exceeds its pore size, whereas NaA and KA zeolites cannot though they have similar pore sizes. Moreover, ZIF-8 selectively adsorbed NNK in solution and reached adsorption equilibrium within 5 min. In contrast, NNN was hard to be adsorbed and easy to be eluted by water, which is useful for the separation of TSNA. The influence of solid-liquid (S/L) ratio and other factors on TSNA liquid adsorption was investigated in detail. When S/L was 0.2, ZIF-8 exerted the highest efficiency and the adsorption capacity per unit surface area reached 12–15 nmol. Through the comparison of adsorption of NNK, NNN and NPYR, it is found that the adsorption of TSNA by ZIF-8 depended on π-π electron interaction mainly, rather than geometric confinement effect. The liquid adsorption curve of NNK conforms to Langmuir isothermal adsorption model, and its saturated adsorption capacity Q m was estimated to be 4.34 mg g −1 , but actually it could reach 3.19 mg g −1 only. In addition, ZIF-8 had a mixing effect in liquid adsorption of TSNA: the presence of NNK promoted the adsorption of NNN slightly. ZIF-8 has the characteristics of fast adsorption and easy elution of TSNA in solution, which is beneficial for removal of TSNA to protect the environment and public health.
研究领域:
环境科学
·
公共卫生
·
材料科学
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DOI
高影响因子
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