FITOTERAPIA
·
2015
·
IF 2.345
Hui Xiong,Yanan Zheng,Guangzhong Yang,Huixia Wang,Zhinan Mei
The phytochemical investigation of the ethanol extract from the stems of Entada phaseoloides (L.) Merr (also called “Guo Gang Long”) led to the isolation of eleven triterpene saponins ( 1 – 11 ). Their structures were elucidated by extensive spectroscopic methods including 1D- ( 1 H and 13 C) and 2D-NMR ( 1 H– 1 H COSY, HSQC, HMBC, HSQC-TOCSY and ROESY) experiments as well as ESIMS analysis and hydrolysis. These saponins comprised entagenic acid as the main aglycon , saccharide moieties at C-3 and C-28, and esterification of C-2 or C-3 hydroxyl group of the terminal β- d -glucopyranose unit with a monoterpenic acid. To further explain the clinical applications of “Guo Gang Long” for its anti-inflammatory effect, the inhibitory activities on the production of NO of the saponins and the related aglycon, entagenic acid ( 12 ), were evaluated in vitro. The compounds containing a free hydroxyl at C-3 of aglycon ( 1 and 4 ) and entagenic acid showed significant activities against NO production in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells with IC 50 values of 25.08, 20.13 and 23.48 μM, respectively. And the three compounds could also inhibit the release of pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-6 and IL-8.
研究领域:
植物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF PHARMACAL RESEARCH
·
2015
·Vol. 39
·期 2
·154-160
·
IF 2.046
Ma Wei,Zhang Yue,Ding Yun-Yun,Liu Feng,Li Ning
Bioactivity guided phytochemical investigation of the ethanol extract of the medullae of Juncus effusus resulted in the isolation of two new phenanthrenes, 8-hydroxymethyl-2-hydroxyl-1-methyl-5-vinyl-9,10-dihydrophenanthrene ( 1 ), and 5-(1-methoxyethyl)-1-methyl-phenanthren-2,7-diol ( 2 ) together with 15 known phenanthrenoids ( 3 – 17 ). The chemical structures of 1 and 2 were established by a combination of spectroscopic techniques. Compounds 1 – 15 and 17 were evaluated for their cytotoxic activities against five human cancer cell lines (SHSY-5Y, SMMC-7721, HepG-2, Hela and MCF-7) by CCK-8 assay, and their anti-inflammatory activities were also evaluated by inhibition on NO production in LPS-activated murine macrophage RAW 264.7 cells.
研究领域:
植物化学
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药理学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2024
·
IF 5.1
Yajie Ma,Fan Zhang,Yujun Xie,Luyao An,Bo-Li Zhang,Bo-Yang Yu,Renshi Li
Asparagi Radix is a healthy food type with medicinal and edible homology, which is now widely used for improving pulmonary inflammation in clinical. However, the lung-protective effect and the active constituents of Asparagi Radix have yet to be elucidated. Herein, the potential pulmonary protective effect of oligosaccharides was investigated. We firstly identified eighteen oligosaccharides with different degrees of polymerization from Asparagi Radix using HPLC-QTOF MS. Oligosaccharides were found to be simultaneously determined in 20 batches of Asparagi Radix collected from various regions in China by HILIC-ELSD, and oligosaccharides stably exist in this herb. In the present study, we found that AROS significantly reduced NO production, and effectively down-regulated the mRNA expression of IL-6, IL-1β and TNF-α in RAW 264.7 cells, thereby reducing the inflammatory response induced by LPS. AROS also inhibited LPS-stimulated intracellular ROS production. A murine model of lipopolysaccharide (LPS)-induced acute lung injury was used to evaluate the in vivo anti-inflammatory and lung protective efficacy of AROS. AROS could ameliorate the damage of the pulmonary cellular architecture pathological injury and lung edema. AROS significantly decreased the levels of cytokines IL-6, TNF-α and IL-1β, as well as the levels of MPO and MDA, and superoxide dismutase consumption in vivo. This effect of oligosaccharides could explain the traditional usage of Asparagus cochinchinensis as a tonic medicine for respiratory problems and oligosaccharides from Asparagi Radix used as a natural ingredient could play an important role in protecting from lung injury.
研究领域:
药理学
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免疫学
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肺科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Mingyue Zhao,Xin Liu,Yuzhu Hou,Tongtong Yang,Jiaquan Xu,Rui Su
The levels of l-arginine and asymmetric dimethylarginine (ADMA) and the amount of the nitric oxide (NO) production have recently been linked to breast cancer and pharmaceutical effect evaluation. Herein, a method combining electrochemistry and high-resolution mass spectrometry (HRMS) was established and used to study NO metabolism and its modulation by ginsenoside compound K (CK) in breast cancer cells. Platinum nanoparticles-decorated fluorine tin oxide was employed as an electrochemical sensor for in situ detection of NO release, while HRMS was used for the analysis of the NO-related metabolites. Through the combination of the electrochemical and HRMS results, decreases in arginine and NO and increases in ADMA and ornithine were observed after modulation by CK, and two highly correlated metabolic pathways including arginine and proline metabolism and vascular smooth muscle contraction were found. This method offers a new strategy for fast evaluation of pharmaceutical efficacy based on NO metabolism.
研究领域:
分析化学
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生物化学
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药理学
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癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Xiao-Fang Zhang,Hong-Fei Li,Hui Liu,Fang-Lu Wei,Jiao-Xian Du,Ji-Kai Liu,Juan He,Tao Feng
Twenty-nine sesquiterpenoids, including pseudoguaiane-type ( 1 – 11 ), eudesmane-type ( 12 – 23 ), and carabrane-type ( 24 – 29 ), have been identified from the plant Carpesium abrotanoides . Of them, compounds 1 – 4 , 12 – 15 , and 24 – 27 , namely carpabrotins A–L, are twelve previously undescribed ones. Compound 3 possessed a pseudoguaiane backbone with a rearrangement modification at C-11, C-12 and C-13, while compound 4 suffered a carbon bond break between the C-4 and C-5 to form a rare 4,5- seco -pseudoguaiane lactone. Compounds 1 – 3 , 5 , 13 – 16 and 25 – 27 exhibited anti-inflammatory activity by inhibiting NO production in LPS-induced RAW264.7 macrophages with IC 50 values less than 40 μM, while compounds 1 , 2 , 5 , 13 , 14 , 16 , and 25 – 27 showed significant inhibitory activity comparable to that of dexamethasone. The anti-atopic dermatitis (AD) effects of compounds 5 and 16 were tested according to 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in KM mice, and the results revealed that the major products 5 and 16 improved the histological features of AD-like skin lesions and mast cell infiltration in mice. This study suggested that sesquiterpenoids in C. abrotanoides should play a key role in its anti-inflammatory use.
研究领域:
药理学
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植物化学
·
炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2022
·Vol. 6
·期 10
·1269-1281
·
IF 8.683
Xiaohui Wang,Xiaoyue Feng,Mengjie Sun,Mingzhu Wang,Wei Wang,Zhi Yuan
Poor perfusion and low accumulation of drugs caused by abnormal tumor blood vessels significantly reduce the chemotherapy efficacy. Nowadays, the synergistic therapy of vascular normalization and chemotherapy has become an ideal choice to increase the tumor inhibition rate. Delivering an appropriate amount of NO around tumor vessels proved to be an effective vascular normalization strategy. In order to meet the demand for continuous and stable production of NO at the tumor site, herein, we developed a strategy to normalize tumor blood vessels by catalyzing NO generation from endogenous NO donors (RSNO). Concretely, the surface of copper-doped mesoporous silica was modified with PAA by electrostatic interactions to form CuMSN-PAA nanoparticles. The system was simple in structure and could catalyze NO production by exposing the CuMSN surface in response to a slightly acidic environment. In vivo, the NO level in the tumor increased 5.8-fold after CuMSN-PAA treatment, and blood perfusion increased by 70%, which in turn induced vascular normalization. Consequently, DOX accumulation of the CuMSN-PAA group increased 2.4-fold compared with the control group. More importantly, after the synergistic treatment of vascular normalization and chemotherapy, the anti-tumor efficacy was increased by 37.2% compared with the DOX-treated group with no overt toxicity observed.
研究领域:
癌症治疗
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纳米技术
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药学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2023
·
IF 8.6
Xingyu Fan,Botao Chen,Hui Xu,Anqiang Pan,Shuquan Liang,Songwen Tan,Yongju He
Depleting intracellular glutathione (GSH) has emerged as a potent strategy to combat cancer. However, the existing GSH-depleting agents are too toxic or ineffective and standalone GSH depletion fails to yield a satisfactory curative effect. Herein, we present an intelligent nanoparticle that possesses GSH depletion, glucose consumption accompanied with H2O2 production, and NO generation properties for multimodal cancer therapy. The nanoparticle is constructed by synthesis of tetrasulfide bond-doped mesoporous silica nanoparticles followed by conjugating glucose oxidase (GOx) on the surface and loading l-arginine (l-Arg) into the mesopores. In this nanoparticle, the doped tetrasulfide bonds can quickly deplete GSH, which increases the cellular reactive oxygen species concentration to induce ferroptosis and meanwhile triggers particle biodegradation to expose the loaded l-Arg. Moreover, the elevated H2O2 level activates l-Arg to release NO for NO therapy. GOx consumes glucose to initiate starvation therapy and simultaneously produces a large amount of H2O2. Importantly, the produced H2O2 can not only potentiate ferroptosis but also promote NO release to enhance NO therapy. Besides, NO could in turn improve the efficacy of starvation therapy by damaging the mitochondria to block energy supply. In vitro and in vivo studies demonstrate that the nanoparticles show a great synergistic effect of ferroptosis/starvation/NO therapy, which can significantly kill cancer cells and remarkably inhibit tumor growth without obvious side effects. Therefore, we think that the designed nanoparticles may provide a promising paradigm for synergistic cancer therapy and hold a prospect in clinical trials.
研究领域:
纳米技术
·
癌症生物学
·
药学
·
治疗学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）