RSC Advances
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2020
·Vol. 10
·期 33
·19300-19308
·
IF 3.119
Wen-Xue Lei,Zi-Shuai An,Bai-Hong Zhang,Qian Wu,Wen-Jun Gong,Jin-Ming Li,Wen-Li Chen
In recent years, gold nanoparticles (AuNPs) have been widely used as gene silencing agents and therapeutics for treatment of cancers due to their high transfection efficiency and lack of cytotoxicity, but their roles in gene silencing in plants have not yet been reported. Here, we report synthesis of AuNPs-branched polyethylenimine and its integration with the small interfering RNAs (siRNA) of NPR1 to form a AuNPs-siRNANPR1 compound. Our results showed that AuNPs-siRNANPR1 was capable of infiltrating into Arabidopsis cells. AuNPs-siRNANPR1 silenced 80% of the NPR1 gene in Arabidopsis. Bacteriostatic and ion leakage experiments suggest that the NPR1 gene in Arabidopsis leaves was silenced by AuNPs-siRNANPR1. In Columbia-0 plants, compared with the control group treated with buffer solution, the AuNPs-siRNANPR1 treatment significantly increased the number of colonies and cell death, and the leaves turned yellow, similar to the phenotype of the npr1 leaves. These results indicated this AuNPs-siRNANPR1 silencing the NPR1 gene method is simple, effective and quick (3 days), and a powerful tool to study gene functions in plants.
研究领域:
植物科学
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分子生物学
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纳米技术
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DOI
PubMed
高影响因子
MOLECULAR PLANT-MICROBE INTERACTIONS
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2020
·
IF 3.696
Huanpeng Li,Jiaojiao Wu,Xiaofeng Shang,Miaomiao Geng,Jing Gao,Shuqing Zhao,Xiumei Yu,Daqun Liu,Zhensheng Kang,Xiaojie Wang,Xiaodong Wang
In Arabidopsis , both pathogen invasion and benzothiadiazole (BTH) treatment activate the nonexpresser of pathogenesis-related genes 1 ( NPR1 )-mediated systemic acquired resistance, which provides broad-spectrum disease resistance to secondary pathogen infection. However, the BTH-induced resistance in Triticeae crops of wheat and barley seems to be accomplished through an NPR1 -independent pathway. In the current investigation, we applied transcriptome analysis on barley transgenic lines overexpressing wheat wNPR1 ( wNPR1-OE ) and knocking down barley HvNPR1 ( HvNPR1-Kd ) to reveal the role of NPR1 during the BTH-induced resistance. Most of the previously designated barley chemical-induced ( BCI ) genes were upregulated in an NPR1 -independent manner, whereas the expression levels of several pathogenesis-related ( PR ) genes were elevated upon BTH treatment only in wNPR1-OE. Two barley WRKY transcription factors, HvWRKY6 and HvWRKY70 , were predicted and further validated as key regulators shared by the BTH-induced resistance and the NPR1 -mediated acquired resistance. Wheat transgenic lines overexpressing HvWRKY6 and HvWRKY70 showed different degrees of enhanced resistance to Puccinia striiformis f. sp. tritici pathotype CYR32 and Blumeria graminis f. sp. tritici pathotype E20. In conclusion, the transcriptional changes of BTH-induced resistance in barley were initially profiled, and the identified key regulators would be valuable resources for the genetic improvement of broad-spectrum disease resistance in wheat.
研究领域:
植物病理学
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分子生物学
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遗传学
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农学
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DOI
PubMed
高影响因子
Experimental and Therapeutic Medicine
·
2023
·Vol. 26
·期 2
·1-10
·
IF 2.7
Yao-Meng Huang,Tong-Xin Li,Shu-Yu Li,Xiao-Ran Zhu,Ying Li,Dang-Yang Liu,Wei-Min Li,Lin-Quan Yang,Kun-Shen Liu,Chao Liu
Natriuretic peptides, which are produced by the heart, bind to natriuretic peptide receptor A (NPR1 encoded by natriuretic peptide receptor 1 gene) and cause vasodilation and natriuresis. Thus, they serve an important role in regulating blood pressure. In the present study, microinjection of CRISPR associated protein 9/single guide RNA into fertilized C57BL/6N mouse eggs was performed to generate filial generation zero (F0) Npr1 knockout homozygous mice (Npr1‑/‑). F0 mice mated with wild‑type (WT) mice to obtain F1 Npr1 knockout heterozygous mice with stable heredity (Npr1+/‑). F1 self‑hybridization was used to expand the population of heterozygous mice (Npr1+/‑). The present study performed echocardiography to investigate the impact of NPR1 gene knockdown on cardiac function. Compared with those in the WT group (C57BL/6N male mice), the left ventricular ejection fraction, myocardial contractility and renal sodium and potassium excretion and creatinine‑clearance rates were decreased, indicating that Npr1 knockdown induced cardiac and renal dysfunction. In addition, expression of serum glucocorticoid‑regulated kinase 1 (SGK1) increased significantly compared with that in WT mice. However, glucocorticoids (dexamethasone) upregulated NPR1 and inhibited SGK1 and alleviated cardiac and renal dysfunction caused by Npr1 gene heterozygosity. SGK1 inhibitor GSK650394 ameliorate cardiorenal syndrome by suppressing SGK1. Briefly, glucocorticoids inhibited SGK1 by upregulating NPR1, thereby ameliorating cardiorenal impairment caused by Npr1 gene heterozygosity. The present findings provided novel insight into the understanding of cardiorenal syndrome and suggested that glucocorticoids targeting the NPR1/SGK1 pathway may be a potential therapeutic target to treat cardiorenal syndrome.
研究领域:
心脏病学
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肾病学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
MOLECULAR PLANT PATHOLOGY
·
2021
·Vol. 22
·期 7
·817-828
·
IF 5.663
Xianping Fang,Weiguo Chai,Shuigen Li,Liqing Zhang,Hong Yu,Jiansheng Shen,Wenfei Xiao,Aichun Liu,Boqiang Zhou,Xueying Zhang
In this study, we used virus-mediated gene silencing technology and found that the HSP17.4 gene-silenced cultivar Sweet Charlie plants were more susceptible to Colletotrichum gloeosporioides than the wild-type Sweet Charlie, and the level of infection was even higher than that of the susceptible cultivar Benihopp. The results of differential quantitative proteomics showed that after infection with the pathogen, the expression of the downstream response genes NPR1 , TGA , and PR-1 of the salicylic acid (SA) signalling pathway was fully up-regulated in the wild-type Sweet Charlie, and the expression of the core transcription factor MYC2 of the jasmonic acid (JA) pathway was significantly down-regulated. The expression of the proteins encoded by these genes did not change significantly in the HSP17.4 -silenced Sweet Charlie, indicating that the expression of HSP17.4 activated the up-regulation of downstream signals of SA and inhibited the JA signal pathway. The experiments that used SA, methyl jasmonate, and their inhibitors to treat plants provide additional evidence that the antagonism between SA and JA regulates the resistance of strawberry plants to C . gloeosporioides .
研究领域:
植物病理学
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分子生物学
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植物遗传学
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DOI
PubMed
高影响因子
iScience
·
2022
·
IF 6.107
Junzhe Zhang,Wenxue Lei,Yixuan Meng,Chengqian Zhou,Baihong Zhang,Jun Yuan,Min Wang,Dongqing Xu,Xiangchun Meng,Wenli Chen
Summary Huanglongbing ( HLB ) is a devastating disease of citrus, which is mostly caused by Candidatus Liberibacter asiaticus (C Las ). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle ( Catharanthus roseus ) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI-pNPR1 ( Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1 )-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA NPR1 infiltration. In addition, NPR1-GFP expression in C Las-infected periwinkle leaves was significantly higher than that in healthy periwinkle leaves after infiltration. Our work confirmed that the expression of exogenous NPR1-GFP could reduce the C Las titers by promoting the expression of PR ( pathogenesis related ) genes and ICS ( isochorismate synthase ) gene.
研究领域:
植物学
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遗传学
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分子生物学
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植物病理学
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DOI
PubMed
高影响因子
Science Advances
·
2025
·Vol. 11
·期 39
·
IF 12.5
Yuyu Guo,Cunliang Li,Yao Liu,Hanyi Mao,Yin Zhang,Pascal Genschik,Lili Wang,Shunping Yan
Plants have evolved sophisticated mechanisms to balance growth and defense. The evolutionarily conserved polymerase-associated factor 1 complex (PAF1C) plays multiple roles in transcription. Here, we show that PAF1C regulates the growth-defense tradeoff by repressing defense genes expression in Arabidopsis . Loss of PAF1C leads to increased expression of defense genes, enhanced disease resistance, but compromised growth. Mechanistically, PAF1C binds to defense genes, where it interacts with histone deacetylases, such as HDA6, to promote histone deacetylation, thereby repressing defense genes expression. The plant immune hormone salicylic acid (SA) promotes the interaction between PAF1 and the SA receptor NPR1, which functions as an E3 ubiquitin ligase to mediate the polyubiquitination and degradation of PAF1. Genetically, loss of PAF1 suppresses the immune defects of the npr1 mutant, supporting the notion that PAF1 functions downstream of NPR1. Collectively, this study identifies the NPR1-PAF1C-HDA6 module that regulates the growth-defense tradeoff.
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DOI
PubMed
高影响因子
PLANT DISEASE
·
2020
·Vol. 104
·期 6
·
IF 3.809
Xian Chen,Pedro Laborda,Fengquan Liu
Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae , is one of the most serious diseases of rice. In this study we found that exogenous melatonin can increase rice resistance to BB. Treatment of rice plants with exogenous melatonin (20 µg/ml) increased nitrate reductase, nitric oxide synthase, and peroxidase activity, enabling high intracellular concentrations of melatonin, nitric oxide, and H 2 O 2 . The expression of NPR1 , a key regulator in the salicylic acid signaling pathway, was upregulated more than 10-fold when the plants were challenged with melatonin. Similarly, the messenger RNA level of PDF1.2 , a jasmonic acid–induced defense marker, was 15 times higher in the treated plants than in the control plants. Moreover, three pathogenesis-related proteins, PR1b , PR8a , and PR9 , were upregulated 20-fold in the presence of melatonin. The application of melatonin (100 µg/ml) to soil-grown rice reduced the incidence of BB by 86.21%. Taken together, these results not only provide a better understanding of melatonin-mediated innate immunity to X. oryzae pv. oryzae in rice but also represent a promising cultivation strategy to protect rice against X. oryzae pv. oryzae infection.
研究领域:
植物病理学
·
农学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yao Wang,Liang Yang,Xiao Zhou,Ye Wang,Yijia Liang,Binshao Luo,Yuhao Dai,Zhouling Wei,Shili Li,Rong He,Wei Ding
The exploration of biopolymer-based materials to avoid hazardous chemicals in agriculture has gained enormous importance for sustainable crop protection. Due to its good biocompatibility and water solubility, carboxymethyl chitosan (CMCS) has been widely applied as a pesticide carrier biomaterial. However, the mechanism by which carboxymethyl chitosan-grafted natural product nanoparticles induce tobacco systemic resistance against bacterial wilt remains largely unknown. In this study, water-soluble CMCS-grafted daphnetin (DA) nanoparticles ( [email protected] ) were successfully synthesized, characterized, and assessed for the first time. The grafting rate of DA in CMCS was 10.05 %, and the water solubility was increased. In addition, [email protected] significantly increased the activities of CAT, PPO and SOD defense enzymes, activated the expression of PR1 and NPR1 , and suppressed the expression of JAZ3 . [email protected] could induce immune responses against R. solanacearum in tobacco, including increases in defense enzymes and overexpression of pathogenesis-related (PR) proteins. The application of [email protected] effectively suppressed the development of tobacco bacterial wilt in pot experiments, and the control efficiency was as high as 74.23 %, 67.80 %, 61.67 % at 8, 10, and 12 days after inoculation. Additionally, [email protected] has excellent biosafety. Therefore, this study highlighted the application of [email protected] in manipulating tobacco to generate defense responses against R. solanacearum , which can be attributed to systemic resistance.
研究领域:
植物科学
·
农业生物技术
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纳米技术
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植物病理学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yueyang Zhang,Yue Jiang,Yina Jia,Xiaoyu Pan,Tianrun Zhao,Kaiyue Wang,He Yan,Zhiqing Ma
Background Omphalia lapidescens is a saprophytic and parasitic fungus belonging to the Polypora genus of Tricholomataceae . It has repellent, insecticidal, anti-inflammatory and immunomodulatory effects. Result This study found that the extract of O. lapidescens had significant anti-TMV activity, and the main active component was homopolysaccharide LW-1 by Bioassay-guided fractionation. LW-1 is a glucan with β-(1,3) glucoside bond as the main chain and β-(1,6) glucoside bond as the branch chain, with molecular weight in the range of 172,916–338,827 Da. The protective and inactive efficacies of LW-1(100 mg/L) against TMV were 78.10% and 48.20%, but had no direct effect on the morphology of TMV particles. The results of mechanism of action showed that LW-1 induced the increase of the activity of defense enzymes such as POD, SOD and PAL in Nicotiana glutinosa . The overexpression of resistance genes such as NPR1, PR1 and PR5 , and the increase of SA content. Further transcriptome sequencing showed that LW-1 activated MAPK signaling pathway, plant-pathogen interaction pathway and glucosinolide metabolic pathway in Arabidopsis thaliana . Besides, LW-1 induced crops resistance against plant pathogenic fungi. Conclusion Taken together, the anti-TMV mechanism of LW-1 was to activate MAPK signaling pathway, inducing overexpression of resistance genes, activating plant immune system, and improving the synthesis and accumulation of plant defencins such as glucosinolide. LW-1-induced plant disease resistance has the advantages of broad spectrum and long duration, which has the potential to be developed as a new antiviral agent or plant immune resistance inducer.
研究领域:
植物病理学
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分子生物学
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植物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）