Materials Express
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2021
·Vol. 11
·期 7
·1057-1063
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IF 0
Tian Jinjing,Lu Xiufang,Cheng Yixin,Fang Zhongwei,Ren Gengpu,Liu Nuofan
Amino terminal pro-brain natriuretic peptide (NT-proBNP) is an important biomarker for clinical diagnosis, risk stratification, prognosis evaluation, and efficacy monitoring in patients with acute and chronic heart failure. Traditionally, NT-proBNP detection is based on immunochromatography,
which is time-consuming, tedious, requires professional-trained operators, etc. Here we developed a simple, fast, unlabeled, highly sensitive, and low-cost carbon nanotubes (CNTs)@Au NPs-modified electrochemiluminescence NT-proBNP sensor, and evaluated its feasibility in the clinical monitoring
of cardiac function in patients with heart failure. Notably, the sensor has a small batch size difference and good reproducibility and can be used for mass production. The device offers a new opportunity and direction for the clinical monitoring of NT-proBNP.
研究领域:
医学诊断
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生物传感
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纳米技术
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DOI
RSC Advances
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2023
·Vol. 13
·期 16
·10503-10507
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IF 3.9
Chenlong Jiang,Xiangde Lai,Feng Han,Zhijun Gao,Haixia Yang,Xuan Zhao,Huajie Pang,Bin Qiao,Hua Pei,Qiang Wu
Heart failure (HF) is a major public health problem triggered by heart circulation disorders. Early detection and diagnosis are conducive to the prevention and treatment of HF. Hence, it is necessary to establish a simple and sensitive method to monitor the diagnostic biomarkers of HF. The N-terminal B-type natriuretic peptide precursor (NT-proBNP) is acknowledged as a sensitive biomarker. In this study, a visual detection method for NT-proBNP was developed based on the oxidized 3,3′,5,5′-tetramethylbenzidine (TMB2+)-mediated etching of gold nanorods (AuNRs) and double-antibody-sandwich ELISA. The etching color for different amounts of NT-proBNP was obvious and significant differences could be ascertained based on the blue-shift of the longitudinal localized surface plasmon resonance (LLSPR) of the AuNRs. The results could be observed by the naked eye. The constructed system showed a concentration range from 6 to 100 ng mL−1 and a low detection limit of 6 ng mL−1. This method exhibited negligible cross-reactivity toward other proteins, and the recoveries of the samples ranged from 79.99% to 88.99%. These results demonstrated that the established method is suitable for the simple and convenient detection of NT-proBNP.
研究领域:
生物标志物检测
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纳米技术
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分析化学
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医学诊断
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2023
·Vol. 11
·期 12
·2754-2761
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IF 7
Yu Ji,Shuang He,Yang Chen,Pei Zhang,Jing Sun,Ya Li,Kaida Kuang,Nengqin Jia
A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy)32+@HKUST-1/TPA and Ce2Sn2O7/K2S2O8 probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy)32+, effectively improving the anodic signal intensity, while the emerging Ce2Sn2O7 emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy. This dual-signal immunosensor has a wide linear range (5 × 10−4–1 × 104 ng mL−1) and a low quantitative detection limit, simultaneously showing high sensitivity, stability and reproducibility, as well as the detection capability of actual serum samples. This dual signal-calibrated immunoassay platform not only reduces the false positive rate of detection results but also provides a promising method for the early diagnosis of heart failure.
研究领域:
生物医学工程
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分析化学
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心血管疾病诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Qian Luo,Xiaomin Qian,Xiaona Mi,Yifeng Tu
A new type of immunosensor was prepared by using a gold nanoparticles/aminozeolitic imidazolate framework-67 nanocomposite (AuNPs/ZIF-67) as the sensing matrix and luminol as the signaling probe to detect the heart failure biomarker N -terminal probrain natriuretic peptide (NT-proBNP). The hybrid nanocomposite was deposited on the surface of indium tin oxide (ITO)-coated glass via the linkage of 3-aminopropyltrimethoxysilane (APTMS); thereafter, the NT-proBNP antibody was immobilized to endow biological specificity, and bovine serum albumin was used to block residual active sites. The resultant sensor has an excellent response when NT-proBNP was attached to the antibody via immunoreaction; here, the output signal was reduced because of the depressed mass transportation of the electrochemiluminescence (ECL) probe and electron transference. The resultant ECL immunosensor displayed a broad linear range from 10 pg/mL to 50 ng/mL, and the low detection limit was 0.74 pg/mL. The ECL immunosensor also has good specificity, stability and reproducibility and can be used to detect NT-proBNP in real blood samples.
研究领域:
生物化学
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纳米技术
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分析化学
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医学诊断
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Haixia Yang,Xiangde Lai,Chenglong Jiang,Xuan Zhao,Huajie Pang,Dongxia Li,Zhijun Gao,Bin Qiao,Feng Han,Jia Tian
Heart failure (HF) is the leading cause of death from cardiovascular disease, and early diagnosis using serum markers holds extraordinary significance for the treatment of patients. N-terminal brain natriuretic peptide (NT-proBNP) is the gold standard for HF diagnosis. Herein, a novel, rapid, sensitive, and immune-sensing strategy for NT-proBNP is reported employing an enzyme-linked immunosorbent assay (ELISA) that combines the use of magnetic biological probes and the fluorogenic substrate Ampliflu Red. Functionalized immunomagnetic nanoparticles with large surface areas provide an abundant reaction platform and reduce the analysis time due to their superparamagnetic properties. Ampliflu Red is catalyzed by horseradish peroxidase (HRP) using hydrogen peroxide (H2O2) to produce strong fluorescence and a low background. The reported method achieved a good detection limit of 2.73 ng mL−1 and a wide linear range from 3.13 to 100 ng mL−1. The analysis time was less than 1 h, which is superior to that of traditional ELISA (3–4 h). In addition, the recoveries of spiked human serum were satisfactory, indicating that the proposed strategy has potential applications for the rapid determination of NT-proBNP and other biomarkers in clinical settings.
研究领域:
心血管疾病
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医学诊断
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生物化学
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纳米技术
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临床化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2018
·
IF 8.173
Jinhui Feng,Faying Li,Xiaojian Li,Yaoguang Wang,Dawei Fan,Bin Du,Yueyun Li,Qin Wei
A new, photoelectrochemical immunosensor was proposed on the basis of the La-CdS/3D ZnIn 2 S 4 /Au@ZnO sensitization structure for detection of aminoterminal pro-brain natriuretic peptides (NT-proBNP). The Au@ZnO-modified electrode was first assembled with 3D ZnIn 2 S 4 , and then further deposited with lanthanum doped cadmium sulfide (La-CdS) via successive ionic layer adsorption and reaction strategy. The Au@ZnO has excellent photoelectric activity and electrical conductivity . The ZnIn 2 S 4 with 3D architectures not only exhibit high photocurrent intensity under visible-light irradiation but also have large surface for the La-CdS deposition. Meanwhile, the La-CdS doped structure could depress the charge recombination, which effectively promotes separation of the generated electron-hole (e - /h + ) pairs and consequently enhances the photocurrent conversion efficiency. The polydopamine (PDA) was used not only as a cross-linker reagent for the immobilization of the anti-NT-proBNP but also as an electron donor for promoting the photo-generated e - /h + separation of the semiconductors. Under optimal conditions, the well-designed photoelectrochemical immunoassay exhibited a low detection limit of 0.32 pg mL −1 and a wide linear range from 0.8 pg mL −1 to 45 ng mL −1 for target NT-proBNP detection. Meanwhile, it also presented good reproducibility, specificity, and stability and might open a new promising strategy for the detection of other important tumor markers.
研究领域:
生物传感器
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分析化学
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材料科学
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光化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 46
·7634-7642
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IF 4.776
Dawei Fan,Chunzhu Bao,Xin Liu,Dan Wu,Yong Zhang,Huan Wang,Bin Du,Qin Wei
A unique hierarchical mesoporous SnO2 microflower was utilized for the fabrication of an immunosensor. The modified nitrogen-doped carbon quantum dots (NCQDs) improved the photoelectrochemical (PEC) activity of the SnO2, and contributed to the in situ assembly of Bi2S3 nanoparticles on the surface of the SnO2 materials. Under the stimulation of visible light and ascorbic acid as an efficient electron donor, the NCQDs and Bi2S3 co-sensitized mesoporous SnO2 microflower (SnO2/NCQDs/Bi2S3) modified ITO electrode exhibited an excellent photoelectric signal arriving at around 150 μA. Moreover, a novel label-free PEC sensor based SnO2/NCQDs/Bi2S3 composite was constructed, which enabled the ultrasensitive detection of N-terminal pro-B-type natriuretic peptide (NT-proBNP). The photocurrents decreased linearly with the logarithm of NT-proBNP concentration in the range of 0.01–50 ng mL−1 with a detection limit of 3.7 pg mL−1 (S/N = 3). Furthermore, the novel PEC immunosensor showed perfect selectivity, good stability and reproducibility, which suggest promising applications in the sensing and clinical diagnosis of cardiac disease.
研究领域:
分析化学
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生物传感器
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心脏病学
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光电化学
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DOI
高影响因子
Journal of Analysis and Testing
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2023
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IF 4.7
Gong Xue,Wu Jiangling,Zhang Jiajia,Jiang Zhongwei,Wang Yi,Zhang Pu
Heart failure (HF) is a clinical hallmark of cardiovascular syndrome leading to substantial morbidity, repeated hospitalization, and enormous healthcare expenditure. As elderly populations continue to increase, biomarker-guided diagnosis and treatment for age-related cardiac decline have become essential. Accumulating evidence demonstrates that N-terminal proBNP (NT-proBNP) can provide a unique window into the diagnosis and risk stratification with HF. Herein, a simple yet robust aptasensor is developed for on-site recognizing the NT-proBNP by its targeting aptamer, thus achieving the accurate diagnosis of HF. This aptasensing system is prepared by absorbing the fluorophore-labeled aptamer strand onto the graphene oxide (GO), leading to efficient quenching without possible off-site signal leakage. The aptamer strand can specifically identify target NT-proBNP molecules via a versatile conformational transformation, resulting in the desorption of the aptamer-NT-proBNP complexes from GO and re-generation fluorescence signal, thus allowing sensitive detection of NT-proBNP in 37 clinical blood samples. Taking advantage of the high specificity of aptamer-guaranteed recognitions of NT-proBNP, this aptasensor system readily achieves better diagnostic performance for HF than commercially adopted chemiluminescence immunoassay (Siemens, CLIA) in hospitals in terms of accuracy (89.2% vs 83.8%), specificity (89.5% vs 84.2%), and positive predictive value (88.9% vs 83.3%). This work provides a stable option for the diagnosis and treatment of elderly HF-related diseases in clinics.
研究领域:
医学
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生物技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Luhai Wang,Jiaze Sun,Siwei Dai,Pengfei Zhang,Yefei Zhu,Yu Zhang
Myocardial fibrosis is a pathological condition characterized by the excessive accumulation of extracellular matrix proteins, primarily collagen, within the myocardium. Early symptoms of myocardial fibrosis are often subtle, leading to late detection. As the disease progresses, it is often associated with life-threatening complications. Therefore, myocardial fibrosis urgently demands a diagnostic system that is rapid, effective, and preferably non-invasive. In this study, we developed a highly sensitive microfluidic light-initiated chemiluminescent assay (LICA) for the simultaneous detection of cTnI, NT-proBNP, HA, LN, PICP, and PIIINP, the key biomarkers involved in the occurrence and development of myocardial fibrosis. The system is highly integrated and compact, incorporating reagent lyophilization and whole blood separation technologies, and is suitable for point-of-care testing. By combining advantages of centrifugal microfluidics and LICA, the system effectively shortens the detection time for key biomarkers associated with MACEs. Moreover, the system provides optimal analytical performance, with LoDs of 0.0018 ng mL−1, 0.0127 ng mL−1, 3.52 ng mL−1, 6.88 ng mL−1, 4.68 ng mL−1, and 0.072 ng mL−1 for cTnI, NT-proBNP, HA, LN, PIIINP and PICP, respectively. Ultimately, the system can achieve an AUC of 0.548, 0.670, 0.772, 0.752, 0.833 and 0.713 for cTnI, NT-proBNP, HA, LN, PIIINP, and PICP, respectively, and an overall combined ROC of 0.946 for the detection of myocardial fibrosis, indicating its excellent efficacy and potential for widespread clinical applications.
研究领域:
医学诊断
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生物工程
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心血管病学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Biaobiao Wang,Haoyu Wang,Xingbo Yu,Sitong Wu,Xing Guo,Jianlong Ji,Shengbo Sang,Chuangang Zhou,Dong Zhao,Deliang Shen
Integrates FeGa magnetostrictive effect to eliminate gravity induced interference and amplify film deformation for enhanced biosensor sensitivity. • Employs detection antibody based dual signal amplification, achieving a low NT-proBNP LOD of 0.01 pg/mL in PBS. • Exhibits stable linear ranges (0.01–1.5 pg/mL and 1.6–15 pg/mL) and high recoveries (96%–106%) in pooled saliva. • This product exhibits excellent anti-interference performance and long-term stability for non-invasive heart failure diagnosis. While the low concentration of salivary N-terminal pro-B-type natriuretic peptide (NT-proBNP) has hindered its use in early diagnosis and monitoring of heart failure (HF), its non-invasiveness and convenience are favored by many. In this study, a biosensor based on multi field coupling and sandwich immunoassay with matched capture and detection antibodies has been developed for high-sensitivity detection of NT-proBNP. Based on the magnetostrictive effect of Fe Ga alloy under magnetic field, the gravity interference of the sensitive layer in traditional sensors is eliminated, the deformation of the thin film is amplified, and the sensitivity of the sensor is improved. In addition, by adding detection antibodies to achieve secondary binding of antigens and antibodies, the surface stress is further amplified, reducing the detection limit of the sensor to 0.01 pg/mL. In addition, the accurate detection of NT-proBNP in pooled saliva samples (PSS) indicates that the prepared biosensor has good practicality. All these results show that the multi field coupling and the dual-antibody sandwich strategy contributes to improving the performance of the biosensors and provides a platform for high-sensitivity detection of clinical biomarkers. Download: Download high-res image (144KB) Download: Download full-size image
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DOI
高影响因子
Small
·
2025
·
IF 12.1
Qinglian Liu,Jianglin Wu,Jiajia Zhang,Fuan Wang,Zhongwei Jiang,Yi Wang,Xue Gong,Pu Zhang
Heart failure (HF) is a globally threatening cardiovascular disease associated with poor quality of life and high mortality, therefore, timely diagnosis and risk prediction for HF disease are urgently needed. Herein, a compact yet robust self-regenerated hybridization circuit (SHC) aptasensor is developed for the amplified detection of N-terminal pro-brain natriuretic peptide (NT-proBNP), a “gold standard biomarker” for HF. The aptamer transduction module can specifically recognize NT-proBNP, thus initiating the cascade hybridization reaction with the successively self-regenerated triggers that reversely initiate the cross-hybridization reaction. Benefiting from the aptamer-specific recognition and the self-replicated signal amplification, the robust SHC aptasensor demonstrated a more impressive diagnostic performance for HF in elderly patients than the clinical fluorescence immunochromatography assay (FICA) in terms of positive predictive value (21 vs 17), specificity (39 vs 32), and diagnostic accuracy (37 vs 36). Furthermore, this approach allows for differentiation among HF patients with varying disease severities, achieving a sufficiently high accuracy of 78.3%, thus facilitating more timely and accurate therapeutic intervention. The versatile and reliable SHC system offers a new approach to analyzing low-abundance biomarkers from clinical rare specimens, which is highly important for early disease diagnosis and prognosis assessment.
研究领域:
医学
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生物传感
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心血管病学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Yan Gao,Junpeng Shi,Xia Sun,Chen Wu,Longlong Cao,Xiaofang Luo,Jinyuan Wang,Xiao Zhang,Yun Zhang
Fluorescent immunochromatographic test strips are critical for highly sensitive, rapid detection. However, their sensitivity for detecting trace biomarkers is often affected by autofluorescence and photon absorption of the samples (nitrocellulose membrane substrates and blood chromophores). Here, we report for the first time an immunochromatographic test strip based on near-infrared (NIR) persistent luminescence nanoparticles (PLNPs) for the highly sensitive detection of the biomarker N-terminal pro-brain natriuretic peptide (NT-proBNP). NIR PLNPs exhibit long NIR persistent luminescence (PersL) properties after excitation, allowing separation of excitation and detection, thereby eliminating sample autofluorescence interference. In addition, the PersL in the NIR region greatly reduces the absorption of photons by the sample, further improving the detection sensitivity. After optimization, the test strips can detect NT-proBNP at levels as low as 15.28 pg/mL, which covers the required clinical detection range. The assay demonstrated a recovery rate of 94–101% in human serum samples, and the inter and intrabatch coefficients of variation were less than 15%. The concentrations of NT-proBNP measured in clinical samples correlated well with those of the Roche electrochemiluminescence immunoassay, which is widely used in clinical practice. This NIR PersL immunochromatographic test strip has potential applications in the highly sensitive and immediate detection of trace biomarkers.
研究领域:
生物医学工程
·
临床检验
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）