Applied Sciences-Basel
·
2021
·Vol. 11
·期 19
·9127
·
IF 2.679
Tianji Liu,Yitong Wang,Junguo Li,Qing Yu,Xiaoman Wang,Di Gao,Fuping Wang,Shuang Cai,Yanan Zeng
The effects of Ca2+in converter slag and electric arc furnace slag leaching solutions on CO2content, the effects of CO2content in solutions on the accumulation of chlorophyll a ofNannochloropsissp., and the mechanisms were studied. Results showed that addition of 50 mg/L converter slag leaching solution and 400 mg/L electric arc furnace slag leaching solution (lower than 50 vol% concentration) promoted the accumulation of chlorophyll a ofNannochloropsissp., while an increased concentration of 70 vol% inhibited its accumulation. The highest concentration of chlorophyll a was obtained on the addition of 10 vol%. With 10 vol% leaching solutions added, chlorophyll a concentration for converter slag reached 4.2 mg/L, 1.45 times as much as that of pure F/2 medium, and chlorophyll a concentration for electric arc slag reached 3.2 mg/L, 1.10 times as much as that of pure F/2 medium. Under the same addition proportion, the promoting or inhibiting effect from converter slag on the accumulation of chlorophyll a ofNannochloropsissp. was more obvious than that for electric arc furnace slag. The reason for the promotion of chlorophyll a accumulation ofNannochloropsissp. was that an increase of Ca2+in the solutions led to an increase of CO2, thereby promoting the photosynthetic rate of microalgae. The study provided new ideas for the green application of solid waste and industrial production of microalgae.Keywords:leaching solutions;converter slag;electric arc furnace slag;Nannochloropsissp.;chlorophyll a;CO2content
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2024
·
IF 5.8
Liqiang Zhang,Ningmin Zhu,Chaochao Wang,Rongzheng Cui,Riyi Lin
Two-step catalytic pyrolysis of Nannochloropsis sp (NS) was conducted with SBA-15 and ZSM-5. Thermogravimetric analyzer (TG) and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) were used to investigate the effects of SBA-15 and ZSM-5 on the two-step pyrolysis of NS. The comparative study showed that ZSM-5 and SBA-15 had different effects on the pyrolysis characteristic of NS because of their different property. ZSM-5 had no effects on the kinetic mechanism, but SBA-15 obviously changed DTG curves and the reaction order of the low temperature pyrolysis stage. SBA-15 increased the contents of amines and alkanes to 19.40% and 5.62% in the first step compared with those of the one-step pyrolysis (1.95% and 1.83%). Due to the stronger acid sites, ZSM-5 improved the content of olefins (33.79%) and esters (10.79%) in the first step and the contents of nitriles (11.75%) and aromatic hydrocarbons (31.01%) in the second step compared with those of one-step pyrolysis (10.61%, 5.41%, 0.00%, 8.42%). Acid catalyst had no positive effects on the generation of N-heterocyclic compounds. This study is expected to provide a reference for the efficient pyrolysis utilization of microalgae.
研究领域:
生物能源
·
催化化学
·
环境科学
✓ 已验证
DOI
高影响因子
ENERGY CONVERSION AND MANAGEMENT
·
2022
·
IF 11.533
Liqiang Zhang,Jiaojiao Liu,Dawei Li,Zhengda Yang,Xinwei Wang,Riyi Lin
To explore an effective method of utilizing nitrogen-rich microalgae, the two-step pyrolysis (TSP) characteristic of Nannochloropsis sp (NCS) over ZSM-5 were studied by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and thermogravimetric analyzer (TG). The results showed that the TG process of NCS was classified into three stages. ZSM-5 increased the final weight loss and the pyrolysis temperature of NCS. The kinetic analysis was conducted by Flynn-Wall-Ozawa method, and showed that the pyrolysis of NCS conformed to one-dimensional shrinking core model. ZSM-5 did not affect the kinetic model, but decreased the activation energy. Compared with one-step pyrolysis, TSP improved the selectivity of N-heterocyclic compounds in the first step. Catalytic TSP further reduced the complexity of products significantly, and increased the selectivity of olefins in the first step, nitriles and aromatic hydrocarbons in the second step. The possible catalytic TSP reaction pathways of NCS were proposed.
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Changyong Cai,Xueying Chen,Fenfang Li,Zhijian Tan
Three-phase partitioning (TPP) had been developed for lipids extraction from microalgae . However, the volatile and flammable organic solvents (typically t -butanol) were widely used in the conventional TPP. In this study, CO 2 -responsive deep eutectic solvents (DESs) were used as the alternative solvents and applied for lipid extraction from Nannochloropsis sp . by using a TPP system. These DESs can be separated from lipid and easily recycled by bubbling CO 2 and N 2 after extraction. The response characteristics of the optimal DES-3 (HBA: HBD = 3:1) were studied by FT-IR, 1 H NMR, and 13 C NMR. Furthermore, factors influencing the extraction process were studied in detail. The maximal extraction yield (172 mg/g) was obtained under the optimized conditions: ammonium sulfate of 20 wt%, liquid–solid ratio of 35:1, extraction temperature of 80 °C, and extraction time of 90 min, which was much higher than the t -butanol-based TPP system (135 mg/g). The lipid was recovered from DES after extraction, and the fatty acid composition was analyzed after TPP. Finally, the recovered DES was reused for lipid extraction, and the results indicated good cyclic stability for this DES after five cycles. This study presents a green, efficient, and sustainable strategy for lipid extraction and further biofuel production.
研究领域:
生物能源
·
绿色化学
·
微藻生物技术
·
提取技术
·
可持续工程
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2018
·
IF 5.807
Qingtai Chen,Dong Liu,Chongchong Wu,Kaisheng Yao,Zhiheng Li,Nan Shi,Fushan Wen,Ian D. Gates
An activity-tunable biocatalyst for Nannochloropsis sp. cell-walls degradation was prepared by co-immobilization of cellulase and lysozyme on the surface of amino-functionalized magnetic nanoparticles (MNPs) employing glutaraldehyde. The competition between cellulase and lysozyme during immobilization was caused by the limited active sites of the MNPs. The maximum recovery of activities (cellulase: 78.9% and lysozyme: 69.6%) were achieved due to synergistic effects during dual-enzyme co-immobilization. The thermal stability in terms of half-life of the co-immobilized enzymes was three times higher than that in free form and had higher catalytic efficiency for hydrolysis of cell walls. Moreover, the co-immobilized enzymes showed greater thermal stability and wider pH tolerance than free enzymes under harsh conditions. Furthermore, the co-immobilized enzymes retained up to 60% of the residual activity after being recycled 6 times. This study provides a feasible approach for the industrialization of enzyme during cell-walls disruption and lipids extraction from Nannochloropsis sp.
研究领域:
生物技术
·
生物化学
·
纳米技术
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）