Nigella glandulifera Freyn et Sint seeds (NG) as well as Nigella sativa L. seeds (NS) have been employed as folk remedies for centuries. However, little work has been done on their quality control. In order to assess the quality and guarantee therapeutic effects, the chemical constituents of the two herbs were extensively characterized by ultra-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and a simple, practical, and reliable HPLC fingerprinting method was established and applied to determination of 20 batches of samples. With their chromatographic fingerprints, the two species were successfully differentiated by pattern recognition methods, namely, principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). Results from this study show that the proposed analytical methods can be applied to the quality control of the seeds and have high potential to ensure their safety and efficacy.
Insulin resistance (IR) is a physiological abnormality that occurs when insulin fails to activate the signal transduction pathway in target organs. It was found that supplementation of Nigella sativa seeds with oral antidiabetic medicines helps improve blood glucose control by enhanced β cells activity and alleviation of IR. However, the activities and related mechanisms of phytochemicals from N. sativa seeds have not been thoroughly explored. In this study, the effects of two triterpenoids, 3-O-[β-D-xylopyranose-(1→3)-α-L-rhamnose-(1→2)-α-L-arabinose]-28-O-[α-L-rhamnose-(1→4)-β-D-glucopyranose-L-(1→6)-β-D-glucopyranose]-hederagenin (Hxrarg) and 3-O-[β-D-xylopyranose-(1→3)-α-L-rhamnose-(1→2)-α-L-arabinose]-hederagenin (Hxra), on IR were studied by 3T3-L1 adipocytes model. The results demonstrated that Hxrarg and Hxra inhibited maturation of 3T3-L1 preadipocytes, dramatically stimulated glucose uptake of IR-3T3-L1 adipocytes, promoted transcription of IRS, AKT, PI-3K, and GLUT4 mRNA. Western Blot results suggested that Hxrarg and Hxra were able to markedly up-regulate expression of p-IRS, p-AKT, PI-3K, and GLUT4 proteins. These findings could provide a basic foundation for the continued development and application of N. sativa in medicine and functional foods.
Kishore Chand,Cheng Jiao,Muhammad Nazim Lakhan,Ahmer Hussain Shah,Vinod Kumar,Diaa Eldin Fouad,Muhammad Basit Chandio,Asif Ali Maitlo,Mukhtiar Ahmed,Dianxue Cao
This study introduces a new method for synthesizes of silver nanoparticles (AgNPs), prepared within Nigella Sativa (NS) extract. NS extract in the process is more advantageous over other processes because NS acts as a highly efficient stabilizing as well as reducing agents due to highly metabolites like flavonoids, terpenoids, proteins etc. These agents are responsible for the formation of AgNPs and contain reducing and stabilizing properties. The as-obtained AgNPs were investigated by various characterization techniques such as Ultra-visible spectroscopy (UV–Vis), X-ray diffraction (XRD), Dynamic light scattering (DLS), Zeta Potential, Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), and high-resolution TEM (HR-TEM) and Brunauer-Emmett-Teller (BET). Fourier transform infra-red (FTIR) analysis was performed to identify the potential bimolecules responsible for the reduction of silver ions. The elemental composition was accomplished by using energy dispersive spectroscopy (EDS). N 2 adsorption-desorption isotherm, pore volume, specific surface area and size of NPs was measured by using BET. The maximum absorbance of UV–Vis was found at 426 nm. TEM, SEM, DLS and BET detected spherical, polydispersed Ag-nano particles with size ranged within 10–12 nm. XRD results demonstrated the formation of well crystallized and smaller nano particles. Furthermore, the synthesized AgNPs exhibited strong photo catalytic activity on degradation of Congo red dye due to high energy and the smaller particles.
危险品化学品经营许可证（带存储）