ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2023
·Vol. 415
·期 20
·4875-4883
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IF 4.3
Lv Junying,Jiao Xiaorui,He Di Demi,Hussain Ejaz,Yang Na,Wang Yongxiang,Zhang Hui,Chen Liping,Jin Xing,Liu Ning,Yu Cong
Cysteine (Cys) is a significant biological mercaptan that achieves key roles in several important physiological processes, such as reversible redox homeostasis in living organisms. Abnormal levels of Cys in the human body are directly related to many diseases. In this work, we constructed a sensitive sensor (Cys-NR) by connecting a Cys recognition group to a Nile red derivative. Due to photo-induced electron transfer (PET), the Cys-NR probe showed little fluorescence at 650 nm. With the addition of Cys to the assay solution, the chlorine unit of the probe was substituted by the thiol group of Cys. Further, the amino and sulfhydryl groups in cysteine underwent an intramolecular rearrangement, which led to the Cys-NR probe water solution turning from colorless to pink with an enhancement in fluorescence. The red fluorescence at 650 nm increased about 20 times. Based on the turn-on signal, a selective Cys detection method is developed. The probe signal is not affected by various potential interferences or other competing biothiols and the limit of detection (LOD) is determined to be 0.44 μM. In addition, the probe is further employed for imaging of Cys in live cells, revealing good biological imaging ability that could provide a new way of intracellular Cys detection. Graphical abstract
研究领域:
分析化学
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生物化学
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医学检测
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PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2025
·
IF 4.7
Bei Jiang,Jintao Jiang,Xinrui He,Yilei Zhu,Chao Guo
Accurate quantification of total antioxidant capacity (TAC) is crucial for evaluating the overall antioxidant activity in biological systems and food products. This is of growing importance in diverse applications such as health risk assessment and nutritional recommendations. Here, we introduce a novel Förster resonance energy transfer (FRET)-based sensor for the determination of TAC. The sensor utilizes an iodine-mediated FRET mechanism, employing BODIPY as the donor and Nile Red as the acceptor, both encapsulated within polystyrene (PS) microspheres. In the presence of iodine, its lipophilicity facilitates adsorption into the PS microspheres, resulting in fluorescence quenching due to the heavy-atom effect. This quenching results in an increased distance between the unquenched donors and acceptors, thereby decreasing FRET efficiency. Conversely, the presence of antioxidants reduces iodine concentration, which increases FRET efficiency. This dynamic allows for the development of a ratiometric fluorescence method for TAC determination. Critically, the sensor demonstrates exceptional photostability and resilience to variations in pH and ionic strength, contributing to its analytical robustness. The proposed method exhibits a linear response to ascorbic acid concentrations ranging from 0 to 50 μM, exhibiting good sensitivity (LOD = 2.5 μM) and selectivity. Additionally, the method has been successfully applied to evaluate TAC in ascorbic acid chewable tablets and lemon juice, demonstrating its potential as a robust and efficient tool for TAC determination.
研究领域:
分析化学
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生物医学工程
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高影响因子
Water
·
2020
·Vol. 12
·期 11
·3251
·
IF 2.544
Heejun Kang,Saerom Park,Bokjin Lee,Jaehwan Ahn,Seogku Kim
Recently, environmental pollution from microplastics (MPs) has become a significant reason for increasing the number of studies to develop analysis methods. The Nile red staining method (NR-S), which is staining polymer particles with Nile red (NR) dye, has been widely used for the analysis of MPs in environmental samples. However, this method has several limitations, as it is difficult to stain MPs covered with organic matter residues. In this study, we modified the NR-S method into an NR plate method (NR-P), where the plate is coated with NR instead of staining MPs directly. The optimum concentration of NR solution was obtained (1000 mg/L), and the effectiveness of the NR-P method for the analysis of MPs was assessed using different types (polypropylene, polyethylene, polyethylene terephthalate, and polystyrene), sizes (100–1000 µm), and shapes (sphere, fiber, film, and flake) of plastic materials. The NR-P method demonstrated improved resolution in the overall types, shapes, and sizes of MPs and was better than the control (without NR plate method) and NR-S method. In particular, the NR-P method can effectively observe MPs covered with organic matter, which was a major limitation of the NR-S method. Finally, MPs in sewage field samples were analyzed by the NR-P method with an accuracy of 78% confirmed by FT-IR. We demonstrated that this method is a convenient and efficient alternative for identifying MPs, even for field samples.Keywords:microplastics;Nile red staining;Nile red plate;UV light
研究领域:
环境科学
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分析化学
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高影响因子
MARINE POLLUTION BULLETIN
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2021
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IF 5.553
Chun Wang,Lijuan Jiang,Ruiqing Liu,Meilin He,Xiaoyu Cui,Changhai Wang
Monitoring microplastics in the environment based on the Nile red staining protocol has proven to be a newly emerged method in several instances. However, the methodology is still having the limitations of susceptibility, indiscrimination, and complexity, etc. The objectives of this paper are to explore the effects of wavelength, temperature, H 2 O 2 and NaCl addition, plastic property, and fluorescent index on the Nile red staining in microplastics analysis and propose solutions to these inadequacies. Sample co-stained with H 2 O 2 (ω final = 10%) and NaCl (ω final = 8.8%) will lower the fluorescence intensity of biogenic materials and reduce their interferences. Based on the fluorescence color and intensity of fused fluorographs, the combined fluorescent index for twelve microplastics was significantly different, thus could be preliminarily distinguished. An elevated staining temperature is propitious to fluorescent tagging with Nile Red. Finally, an improved protocol was proposed, which made the methodology streamlined in microplastics analysis.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2016
·Vol. 16
·期 6
·5569-5576
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IF 1.338
Li Qinqin,Li Chenglin,Tong Weijun
Here, a novel poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) for magnetic resonance (MR) and fluorescence imaging was developed for cell imaging. PLGA NPs loaded with fluorescent dye Nile red (NR) and surface-coated with poly(ethyleneimine) (PEI) were produced in a single
step nanoprecipitation process. Diethylenetriamine pentaacetic dianhydride (DTPA) was conjugated to PLGA/NR@PEI NPs through amidation reaction between –COOH of DTPA and –NH2 of PEI, which can chelate gadolinium (Gd3+) as an MR imaging contrast agent. The PLGA/NR@PEI-DTPA-Gd
NPs exhibited a uniform particle size of ∼200 nm and were stable in culture medium. These NPs had a high T
1 relaxivity (R
1) of 28.36 mM−1S−1. They did not introduce serious cytotoxicity against A549 lung cancer cells.
Furthermore, fluorescence and MR imaging studies on A549 lung cancer cells in vitro revealed that PLGA/NR@PEI-DTPA-Gd NPs can serve as an efficient fluorescence/MR dual-modality imaging nanoprobe.
研究领域:
纳米技术
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生物医学工程
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材料科学
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医学成像
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癌症研究
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PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
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IF 6.723
Ruoyan Wei,Zuwu Wei,Lining Sun,Jin Z. Zhang,Jinliang Liu,Xiaoqian Ge,Liyi Shi
Iron ion (Fe3+) which is the physiologically most abundant and versatile transition metal in biological systems, has been closely related to many certain cancers, metabolism, and dysfunction of organs, such as the liver, heart, and pancreas. In this Research Article, a novel Nile red derivative (NRD) fluorescent probe was synthesized and, in conjunction with polymer-modified core–shell upconversion nanoparticles (UCNPs), demonstrated in the detection of Fe3+ ion with high sensitivity and selectivity. The core–shell UCNPs were surface modified using a synthesized PEGylated amphiphilic polymer (C18PMH-mPEG), and the resulting mPEG modified core–shell UCNPs (mPEG-UCNPs) show good water solubility. The overall Fe3+-responsive upconversion luminescence nanostructure was fabricated by linking the NRD to the mPEG-UCNPs, denoted as mPEG-UCNPs-NRD. In the nanostructure, the core–shell UCNPs, NaYF4:Yb,Er,Tm@NaGdF4, serve as the energy donor while the Fe3+-responsive NRD as the energy acceptor, which leads to efficient luminescence resonance energy transfer (LRET). The mPEG-UCNPs-NRD nanostructure shows high selectivity and sensitivity for detecting Fe3+ in water. In addition, benefited from the good biocompatibility, the nanostructure was successfully applied for detecting Fe3+ in living cells based on upconversion luminescence (UCL) from the UCNPs. Furthermore, the doped Gd3+ ion in the UCNPs endows the mPEG-UCNPs-NRD nanostructure with effective T1 signal enhancement, making it a potential magnetic resonance imaging (MRI) contrast agent. This work demonstrates a simple yet powerful strategy to combine metal ion sensing with multimodal bioimaging based on upconversion luminescence for biomedical applications.
研究领域:
生物医学
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化学
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材料科学
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Zhaoyan Yang,Gujie Pei,Jinlin Zhang,Bing Chen,Yeming Qing,Kuo Yang,Dan Zhu,Bingxiang Li,Zhuyuan Wang
Background Nanoplastics are progressively recognized as a significant global pollutant and a severe threat to human health. Owing to their tiny particle size and low environmental concentrations, nanoplastics detection currently relies on sophisticated laboratory instrumentation. Colorimetric assays represent a rapid, user-friendly strategy, as the testing results can be readily identified by the naked eye. However, this technique is generally limited by unsatisfactory detection sensitivity. Surface-enhanced Raman scattering (SERS) technique allows for the highly sensitive analysis of samples in aqueous solutions without direct contact. Nevertheless, the complicated preparation of high-performance SERS substrates greatly hinders their practical application in nanoplastics detection. Results Herein, a novel colorimetric/SERS dual-mode sensing strategy was developed via Nile Red labeling for rapid and sensitive detection of nanoplastics (≥100 nm). Nanoplastics labeled with Nile Red (referred to as NPs@NR) demonstrated a visually discernible coloration to the naked eye, as well as an obvious SERS signal under a portable Raman spectrometer. Moreover, the SERS signal of NPs@NR was collected from a straightforward SERS substrate with an RSD of 9.93% that fabricated by transferring Ag nanoparticles film (ANF) onto the end of a hydrophilic polyvinylidene fluoride (PVDF) strip in one step. Colorimetric assays were capable of qualitative identification and quantitative detection of NPs@NR at a concentration of 10 μg/mL and higher. Furthermore, the ANF-based SERS substrate achieved a low limit of detection (LOD) of 0.01 μg/mL toward NPs@NR. More importantly, the proposed colorimetric/SERS dual-mode strategy presented excellent feasibility for the detection of nanoplastics spiked in real environmental samples. Significance Given integrating the advantages of facile detection through colorimetric analysis and highly sensitive trace assays using SERS, the proposed dual-mode method demonstrates potential for on-site monitoring of nanoplastics in aquatic environments.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Lulu Lv,Junhao Qu,Zihua Yu,Daihuan Chen,Chunxia Zhou,Pengzhi Hong,Shengli Sun,Chengyong Li
Microplastics (particle size <5 mm) are an emerging contaminant for aquatic environmental, which have attracted increasing attention in worldwide range. In this study, an improved fluorescent staining method for detection and quantification of microplastics was developed based on thermal expansion and contraction. This method is effective in detection of polyethylene, polystyrene , polyvinyl chloride and polyethylene terephthalate plastic particles. In order to avoid error statistics caused by pretreatment , various characterizations of microplastics were measured after heated, such as microstructure, compositions and thermostability. The results showed that there was no significant damage to microplastics even under heating condition at 75 °C for 30 min, and the stained microplastics had strong stability for up to two months. Moreover, this method has been successfully applied to the quantification of microplastics in biological samples and result showed there were about 54 particles g −1 (dry weight) microplastics in the Sipunculus nudus. This new method provides a reliable method for quantitative analysis of microplastics in environment and biological tissue.
研究领域:
环境科学
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生物学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）