Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen
Summary Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling.
CAS / MDL:
64-19-7
研究领域:
生物医学工程
·
组织工程
·
材料科学
·
细胞生物学
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高影响因子
危险品化学品经营许可证（带存储）