NEW JOURNAL OF CHEMISTRY
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2023
·Vol. 47
·期 26
·12200-12207
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IF 3.3
Botao Zhang,Liyun Ding,Yumei Zhang,Shiyuan Wang,Xingdong Jiang,Fei Ma,Jue Zhao
A novel composite fluorescence probe (oPD-CDs@UiO-66-NH2) was successfully prepared for NO detection in the aqueous phase, where carbon quantum dots (oPD-CDs) with NO recognition units were doped in a hydrothermally stable metal–organic framework material (UiO-66-NH2). oPD-CDs and UiO-66-NH2 combined by electrostatic adsorption, and oPD-CDs were uniformly distributed within the pore channel of UiO-66-NH2. This not only suppressed the aggregate induced fluorescence quenching occurring in carbon quantum dots, but also facilitated the efficient detection of NO. The prepared novel fluorescence probes were more sensitive to NO detection than pure oPD-CDs. NO can be detected in a wide linear concentration range of 6.25–500 μM with a lower limit of detection of 4.27 μM. The probes had high selectivity for NO analogs as well as other common substances. This was mainly attributed to the modification of UiO-66-NH2 by oPD-CDs, which can improve the efficient and selective adsorption of NO.
研究领域:
分析化学
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材料科学
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纳米技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Guang-Li Wang,Xue-Lian Hu,Xiu-Ming Wu,Zai-Jun Li
In this paper, a novel and sensitive assembled quantum dots (QDs)–bienzyme (glucose oxidase (GOD) and horseradish peroxidise (HRP)) hybrid system was designed for the direct determination of glucose. Using o-phenylenediamine (OPD) or 3,3′-diaminobenzidine (DAB) as substrate of HRP, whose corresponding oxidation product (oxOPD or oxDAB) effectively quenched the fluorescence of thioglycollic acid (TGA)-capped CdS QDs. The fluorescence quenching of the CdS QDs was proportional to the concentration of glucose and a detection limit of 0.1 μmol/L (or 20 nmol/L) was achieved under the optimum conditions when using OPD (or DAB) as the substrate of HRP. The sensing system was also applied to the determination of glucose in human serum with satisfactory results without any need for complicated sample pretreatments. Other sugars such as fructose, sucrose and lactose hardly interfered on the detection of glucose. The developed method manifested several advantages of high sensitivity, short analysis time, low cost and easy of operation. Moreover, it had great potential in the direct detection of real samples in clinical diagnostics of various diseases.
研究领域:
生物化学
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医学诊断
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纳米技术
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 5
·3851-3856
·
IF 3.201
Shuyun Zhu,Cuihua Lei,Yue Gao,Jing Sun,Hongwei Peng,Han Gao,Ruixue Zhang,Rui Wang,Xian-En Zhao,Hua Wang
Ascorbic acid (AA), as one of the most important neurochemicals in cerebral systems, plays a vital role in many physiological and pathological processes. Herein, a facile, label-free, and ultrasensitive fluorescence sensing system was developed for the determination of AA based on the Ag+–o-phenylenediamine (OPD) interaction. OPD could be oxidized by Ag+ to generate fluorescent 2,3-diaminophenazine (OPDox). When AA was introduced, on the one hand, AA can inhibit the oxidation process due to its strong reducing capability. On the other hand, AA can be oxidized to dehydro-AA with an absorption peak at 380 nm, which has a good spectral overlap with the emission of OPDox. Thus, the inner filter effect (IFE) between dehydro-AA and OPDox may occur. Therefore, the introduction of AA can intensively suppress the fluorescence of the Ag+–OPD system. Benefitting from the remarkable synergistic effect of the reducing capability of AA and IFE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The whole detection procedure was achieved within 10 min. The linear response range of AA was obtained from 0.05 to 40 μM with a detection limit of 10 nM. This developed method has many merits including more simplicity, good selectivity, excellent biocompatibility, and more cost-effectiveness without using any nanomaterials. Notably, the proposed method was successfully applied to detect AA in rat brain microdialysates in the presence of catecholamines, thus providing a new route for AA detection in physiological and pathological fields.
研究领域:
神经化学
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分析化学
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DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 23
·4260
·
IF 5.2
Yuan Fang,Shusen Ding,Weiran Li,Jingjing Zhang,Hui Sun,Xiaodong Lin
Silver ions (Ag+) exist widely in various areas of human life, and the food contamination caused by them poses a serious threat to human health. Among the numerous methods used for the detection of Ag+, fluorescence and colorimetric analysis have attracted much attention due to their inherent advantages, such as high sensitivity, simple operation, short time, low cost and visualized detection. In this work, Pd/Pt nanoflowers (NFs) specifically responsive to Ag+were synthesized in a simple way to oxidize o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAP). The interaction of Ag+with the surface of Pd/Pt NFs inhibits the catalytic activity of Pd/Pt NFs towards the substrate OPD. A novel dual-channel nanosensor was constructed for the detection of Ag+, using the fluorescence intensity and UV-vis absorption intensity of DAP as output signals. This dual-mode analysis combines their respective advantages to significantly improve the sensitivity and accuracy of Ag+detection. The results showed that the limit of detection was 5.8 nM for the fluorescence channel and 46.9 nM for the colorimetric channel, respectively. Moreover, the developed platform has been successfully used for the detection of Ag+in real samples with satisfactory recoveries, which is promising for the application in the point-of-care testing of Ag+in the field of food safety.Keywords:silver ions;Pd/Pt nanoflowers;fluorescent sensor;colorimetric sensor
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Ke Wang,Jian Liu,Xin Wang,Xiaoyang Liu,Jinshuang Hu,Enshou Li,Yusai Zhao,Rusong Zhao,Shenghong Yang
Copper oxide nanoparticles (CuO NPs) as nanozymes have received extensive attention due to their numerous advantages such as water solubility and biomimetic effects. In this work, we combined the inherent oxidase and ascorbate oxidase mimetic activity of CuO NPs to construct a ratiometric fluorescence system for the detection of ascorbic acid (Vitamin C, AA) in serum and fruits. By feat of the dual mimic nanozymatic activity of CuO NPs, the sole substrate o-phenylenediamine (OPD) can be catalyzed to attain yellow fluorescent 2,3-diaminophenazine (oxOPD), while oxidized AA will react with the pristine OPD to form blue fluorescent product. This method has a sensitive response to AA with a good linear relationship in the concentration range of 1–150 μM and low detection limit of 0.16 μM. Practical sample detection of AA in human serum and fruits can be realized reasonably with the recovery rates between 84.83% and 87.28% and 88.53%-113.1%, respectively. More conveniently, the method can be successfully used for scanning measurement analysis through the associated application (ColorAssist) on smartphones and portable UV lamps.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 16
·5554-5561
·
IF 7.059
Qian Yang,Chuyao Li,Jinhua Li,Maryam Arabi,Xiaoyan Wang,Hailong Peng,Hua Xiong,Jaebum Choo,Lingxin Chen
A blue-, yellow- and red-emitting fluorescence sensor was constructed via the synergy of manganese dioxide (MnO2) nanosheets, o-phenylenediamine (OPD) and red-fluorescent quantum dots (r-QDs), namely MnO2–OPD–QD, for visualization of ascorbic acid (AA) and alkaline phosphatase (ALP). Initially, by virtue of the oxidase-like activity of MnO2 nanosheets, OPD was oxidized into yellow-fluorescent OPDox (emission: 569 nm), which quenched the r-QDs (emission: 659 nm). After interaction with AA, one reaction product of ALP and 2-phospho-L-ascorbic acid (AA2P), the MnO2 nanosheets were reduced and lost the oxidase-like activity, so that the generation of OPDox was lessened and the fluorescence of the r-QDs was thereupon recovered. Further increasing AA or ALP, the MnO2 nanosheets were fully decomposed so that OPD was not oxidized but was reduced into blue-fluorescent OPDred (emission: 441 nm), while the r-QDs were not affected. Overall, AA or ALP triggered emission intensity changes at 441, 569 and 659 nm and led to profuse color variation over the yellow–orange–red–purple range, realizing multiplex and visual determination of AA and ALP in the ranges of 0.5–70 μM and 0.1–100 mU mL−1, respectively. The color variation range of the ternary-emission mode was greatly enlarged, especially compared with that of the single-/dual-emission mode. Accordingly, the MnO2–OPD–QD sensor provided detection limits for AA and ALP as low as 0.18 μM and 0.06 mU mL−1, respectively. Furthermore, the highly selective/sensitive measurement and accurate readout of AA and ALP were accomplished in complicated food and serum samples. The multi-emitting fluorescence sensor held great potential for onsite visualization of various targets in more fields.
研究领域:
分析化学
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生物传感器
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材料科学
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生物化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Rui Zhou,Miao Dong,Yiwei Wu
N, Si, S co-doped graphene quantum dots (N, Si, S-GQDs) were synthesized from waste toner and glutathione (GSH) via a one-pot hydrothermal method. Combined with o-phenylenediamine (OPD), a rapid, visual, and autocatalytic ratiometric fluorescence sensor of N, Si, S-GQDs/OPD was fabricated for detecting Ag(I) and Fe(III) ions by producing 2,3-diaminophenazine (oxOPD) under pH 8, which emits yellow fluorescence at 560 nm while quenching the blue fluorescence of N, Si, S-GQDs at 440 nm due to an inner filter effect. With the increase of Ag(I)/Fe(III), the blue fluorescence of N, Si, S-GQDs at 440 nm was gradually weakened, along with the enhancement of yellow fluorescence at 560 nm. Hence, this color change from blue to yellow under UV light enables semi-quantitative visual detection. The sensor demonstrates high sensitivity with detection limits of 0.016 µg mL −1 for Ag(I) and 0.010 µg mL −1 for Fe(III), and it successfully detects these ions in lake and tap water without pretreatment. The autocatalytic mechanism involves Ag(I) and Fe(III) reduction to Ag nanoparticles and Fe(II), respectively, which further catalyze the reaction, enhancing selectivity and efficiency. The method is cost-effective, simple, and suitable for on-site environmental monitoring.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2024
·
IF 3.3
Yaxin Li,Mei Chen,Hongzhi Lu,Shoufang Xu
A straightforward and highly sensitive ratiometric fluorescence sensor comprising blue emissive carbon dots , silver nanoprisms, and o-phenylenediamine (B-CDs-Ag NPRs-OPD) was developed for the detection of H 2 O 2 and glucose. Ag NPRs were etched into Ag + ions upon exposure to H 2 O 2 , which caused non-fluorescent OPD to be oxidized and produce 2,3-diaminophenazine (DAP), resulting in intense yellow fluorescence emission at 555 nm. DAP can quench the B-CDs based on inner filter effect (IFE), leading to a decrease in their fluorescence intensity at 435 nm. Because glucose oxidase may catalyze the oxidation of glucose to produce H 2 O 2 , this approach can also be used to detect glucose. The unique triangular structure leads to the easy etching of the edge and tip of Ag NPRs by H 2 O 2 into Ag + ions, allowing for the high sensitivity of detection of H 2 O 2 and glucose with limit of detection (LOD) of 0.12 μM and 0.6 μM, respectively. Furthermore, with the increase of the concentrations of H 2 O 2 and glucose, a visible color shift from blue to yellow-green occurs, which can be detected using a smartphone's color recognition application that captures the RGB channel values of the image. Real-time and on-spot detection of H 2 O 2 and glucose were achieved based on smartphone sensing platform, presenting vast potential for applications in various fields.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
Journal of Materials Chemistry C
·
2018
·Vol. 6
·期 30
·8105-8114
·
IF 5.976
Ping Gao,Xiang-Ying Sun,Bin Liu,Hui-Ting Lian,Xin-Qiang Liu,Jiang-Shan Shen
Developing a catalyst with a uniform and well-defined crystal structure is very important to establish a catalytic sensing system with high efficiency. In this work, Cu-BTC metal–organic frameworks (MOFs) without a crystalline CuO or Cu2O phase were successfully synthesized by using a low temperature synthesis method, and the as-prepared Cu-BTC MOFs were found to have outstanding catalytic activity towards the traditional oxidase substrate, o-phenylenediamine (OPD), to generate a colored and fluorescent product. The results of the kinetics analysis revealed that the catalytic behavior of Cu-BTC MOFs towards OPD could well follow the typical Michaelis–Menten equation, indicative of a strong affinity between Cu-BTC MOFs and OPD. On the basis of these findings, taken together the condensation reaction between the –CHO group of formaldehyde and the –NH2 group of OPD to form Schiff base compounds, a novel Cu-BTC MOF-based catalytic sensing system for detecting gaseous formaldehyde could be successfully established.
研究领域:
催化
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材料科学
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分析化学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Wenya Hou,Wuyong Zhao,Sijing Yi,Wenmei Gao,Jiangang Zhang,Jinzhong Zhao,Xiaoxia Liu
Papain , as a classical cysteine protease , has been widely used in the food, pharmaceutical, chemical, and cosmetic fields. However, there are few information about the peroxidase-like activity of papain catalyzed substrate to produce fluorescence. In this study, we found that papain can catalyze H 2 O 2 to convert o-phenylenediamine (OPD), and generate fluorescence emission at 550 nm under 430 nm excitation. Based on this foundation, we report a papain/OPD/H 2 O 2 system for fluorescence detection of uric acid. The method exhibits a wide linear range of 10–1000 μM with a limit-of-detection of 4.6 μM, and has been successfully used to detect uric acid in human serum. This study paves the way for the application of papain as catalyst for fluorescence detection of different target biomolecules , such as cholesterol, glucose, lactate, for which H 2 O 2 is a product of oxidoreductase enzymes .
研究领域:
生物化学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 15
·3757-3764
·
IF 5.256
Jiang-Shan Shen,Jia-Jia Wu,Xiang-Ying Sun,Zhen-Long Wu,Ping Gao,Bin Liu
We found that β-HgS nanocrystals could catalyze the reactions of oxidase substrates such as 3,3′,5,5′-tetramethylbenzidine (TMB) and O-phenylenediamine (OPD) to form coloured and fluorescent products, respectively, but α-HgS nanocrystals could not catalyze these reactions. Further investigation revealed that the predominantly exposed crystal planes may play an important role in determining the catalytic activity. On the basis of these findings, taken together with the reaction of forming a Schiff base between the –NH2 group of OPD and the –CHO group of formaldehyde, a novel, simple, and highly selective photoluminescence (PL) sensing platform for detecting formaldehyde could thereby be successfully developed.
研究领域:
纳米技术
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分析化学
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材料科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Jiaxi Deng,Yiyao Luo,Jia Zheng,Zirui Yuan,Yi Ma,Huibo Luo,Xiaogang Luo,Danqun Huo,Changjun Hou
Baijiu represents the pinnacle of traditional Chinese culinary culture. The influence of Baijiu is evident in various literary works, rituals and banquets, dishes and healthcare, and numerous other areas. The reduction of substances plays a pivotal role in the flavor, aroma, and quality control of Baijiu. Therefore, the present work focuses on Baijiu and the reducing substances in it. In this study, CDs@ZrFe-MOF and three distinct catalase nanase substrates (ABTS, OPD, and TMB) were employed to create fluorescence and colorimetric dual-mode sensor arrays. The oxidation of the enzyme substrate into the appropriate oxidation substrate is facilitated by CDs@MOF in the presence of H₂O₂. The reducing substance effectively inhibits the formation of the oxidation substrate, resulting in alterations in fluorescence and UV intensity. The dual-mode sensor array was found to be effective in detecting 15 different types of reducing chemicals. Furthermore, the colorimetric and fluorescence sensor array demonstrated exceptional detection capability in detecting 19 different types of Luzhou-flavor liquor samples, achieving a 100% accuracy rate. This study will provide an accurate and rapid method for differentiating Baijiu.
研究领域:
分析化学
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纳米技术
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传感器技术
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）