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Modulation of the catalytic performance of OYE3 by engineering key residues at the entrance of the catalytic pocket

Shijin Wu,Qiang Wang,Xiaojing Ma,Lequan Qiu,Hongde Yan

The amino acid residues at the entrance of the catalytic pocket may impose steric hindrance on the substrate to enter the active center of the enzyme. Based on the analysis of the three-dimensional structure of the Saccharomyces cerevisiae old yellow enzyme 3 (OYE3), four bulky residues were chosen and mutated to small amino acids. The results showed that mutation of the W116 residue had interesting impacts on the catalytic performance. All four variants became inactive for the reduction of ( R )-carvone and ( S )-carvone, but inverted the stereoselectivity for the reduction of ( E / Z )-citral. The mutation of the F250 residue had a more positive effect on the activity and stereoselectivity. Two variants, F250A and F250S, showed excellent diastereoselectivity and activity for the reduction of ( R )-carvone (de > 99%, c > 99%) and increased diastereoselectivity and activity for the reduction of ( S )-carvone (de > 96%, c > 80%). One variant of the P295 residue, P295G, displayed excellent diastereoselectivity and activity only for the reduction of ( R )-carvone (de > 99%, c > 99%). Mutation of the Y375 residue had a negative impact on the activity of the enzyme. These findings provide some solutions for rational enzyme engineering of OYE3.