TALANTA
·
2023
·
IF 6.1
Pengfei Ma,Hualin Guo,Ke Li,Yin Zhang,Huiqing Guo,Zhouping Wang
Patulin (PAT) and ochratoxin A (OTA) are the two main mycotoxins present in apples. Herein, a sensitive aptasensor for simultaneous detection of PAT and ochratoxin OTA was developed. Dual-color gold nanoclusters (AuNCs) with enhanced fluorescence properties were synthesized and employed as fluorescence amplifiers. Two separated fluorescence peaks at 650 nm and 530 nm were monitored simultaneously by employing single excitation (405 nm), corresponding to the aptamer probes of Cys@BSA-AuNCs-Apt PAT and Arg@ATT-AuNCs-Apt OTA , respectively. The fluorescent aptasensor demonstrated satisfying specificity, storage ability and accuracy. Under the optimal experimental conditions, the linear detection range for PAT and OTA was 0.10–50 ng/mL, with the limit of detection of 0.09 ng/mL and 0.06 ng/mL, respectively. Most importantly, practicability of the constructed aptasensor were confirmed by conducting the determination of PAT and OTA in apple juice sample, indicating the great potential of the aptasensor in practical detection applications.
研究领域:
食品科学
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分析化学
·
生物技术
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DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Tongfu Huang,Mei Wang,Nian Hong,Hanfeng Cui,Qiqi Fan,Guobing Wei,Longshua Qin,Jing Zhang,Hao Fan
Herein, an autonomous driven DNA walker-based aptasensor is proposed for the detection of Ochratoxin A (OTA). A nanoscale metal-organic framework (MOF) is synthesized and used to load Mn 2+ , a cofactor for DNAzyme. The DNA walker and Mn 2+ @MOF are assembled on the Au surface, integrating the DNA walker and metal auxiliary ions. The proposed sensor avoids the addition of metal auxiliary ions of DNAzyme from the external environment, which achieves the fully autonomous driving of DNA walker and facilitates the on-site detection of OTA. In addition, the substrate strands are modified with a newly synthesized tetraferrocene signal marker, further achieving signal amplification. The sensor demonstrates a sensitivity of 0.289 pg/mL and is successfully applied to real food sample analysis.
研究领域:
生物化学
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分析化学
·
食品科学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Weiwei Qiu,Feng Gao,Natsumi Yano,Yusuke Kataoka,Makoto Handa,Weiqiang Yang,Hidekazu Tanaka,Qingxiang Wang
Electrochemical aptasensors involved in chemical labeling are often single-use and sensitivity-limited because the probes are commonly single-point labeled and irreversible. In this work, the specific coordination between Zr4+ and phosphate group (−PO43–) was employed to construct a new aptasensor that is highly sensitive and reusable, using Ochratoxin A (OTA) as the test model. The OTA binding aptamer (OBA) was hybridized with the thiolated supporting sequence (TSS) immobilized on the surface of a gold electrode. The UiO-66 with a formula of [Zr6O4(OH)4(BDC)6], one of the class of Zr metal–organic frameworks (MOFs), was then particularly grafted on the terminal of OBA through the specific coordination between Zr4+ and 5′-PO43–, i.e., the Zr–O–P coordination bond. Similarly, as much as the 5′-PO43– and 3′-methylene blue dual-labeled sequences (DLS) were further assembled on UiO-66 due to the large surface area of MOF and rich active sites of Zr4+. Owing to the specific coordination for signal amplification, the developed aptasensor shows greatly enhanced sensitivity. A wide detection range from 0.1 fM to 2.0 μM and an ultralow detection limit of 0.079 fM (S/N = 3) for OTA were obtained. Additionally, the TSS can rehybridize with a new OBA to regenerate the aptasensor but without complicated pretreatments, enabling a aptasensor that is readily reusable for OTA detection. The aptasensor was successfully applied for OTA detection in the red wine samples, demonstrating a promising prospect for food safety monitoring.
研究领域:
生物化学
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食品安全检测
·
纳米材料科学
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DOI
高影响因子
Toxins
·
2021
·Vol. 13
·期 11
·802
·
IF 4.546
Qian-Wen Zheng,Xu-Fen Ding,Hui-Jun Cao,Qian-Zhi Ni,Bing Zhu,Ning Ma,Feng-Kun Zhang,Yi-Kang Wang,Sheng Xu,Tian-Wei Chen,Ji Xia,Xiao-Song Qiu,Dian-Zhen Yu,Dong Xie,Jing-Jing Li
Ochratoxin A(OTA) is considered to be one of the most important contaminants of food and feed worldwide. The liver is one of key target organs for OTA to exert its toxic effects. Due to current lifestyle and diet, nonalcoholic fatty liver disease (NAFLD) has been the most common liver disease. To examine the potential effect of OTA on hepatic lipid metabolism and NAFLD, C57BL/6 male mice received 1 mg/kg OTA by gavage daily. Compared with controls, OTA increased lipid deposition and TG accumulation in mouse livers. In vitro OTA treatment also promoted lipid droplets accumulation in primary hepatocytes and HepG2 cells. Mechanistically, OTA prevented PPARγ degradation by reducing the interaction between PPARγ and its E3 ligase SIAH2, which led to activation of PPARγ signaling pathway. Furthermore, downregulation or inhibition of CD36, a known of PPARγ, alleviated OTA-induced lipid droplets deposition and TG accumulation. Therefore, OTA induces hepatic steatosis via PPARγ-CD36 axis, suggesting that OTA has an impact on liver lipid metabolism and may contribute to the development of metabolic diseases.Keywords:fatty liver disease;lipid metabolism;OTA;PPARKey Contribution:The in vitro and in vivo study reveal that OTA induces hepatic steatosis via PPARγ-CD36 axis. OTA prevents PPARγ from ubiquitination-mediated degradation by reducing the expression of the E3 ligase SIAH2 and SIAH2-PPARγ interaction.
研究领域:
毒理学
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肝病学
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分子生物学
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Chenxi Yang,Yingying Yang,Wanzhen Xu,Hao Xiong,Lulu Feng,Yongshu Li,Xiaoyue Xiao,Wudan Cai,Huan Liu,Qin Wu,Jianjun Hou,Xixia Liu
The extraction of mycotoxins from complex food matrices for immunoassay often requires organic solvents, which can compromise antibody integrity and detection sensitivity. This study aimed to investigate the tolerance patterns of an ochratoxin a (OTA)-specific nanobody in organic solvents, elucidating the relationship between its structural stability and functional activity. Direct competitive enzyme-linked immunosorbent assay, fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, Fourier transform infrared spectroscopy, and molecular docking were employed to systematically analyze the tolerance thresholds, antibody activity retention rates, and structural changes of the OTA nanobody in eight organic solvents.The results showed that the OTA nanobody exhibited higher tolerance in methanol, ethylene glycol, glycerol, acetone and dimethyl sulfoxide (thresholds of 40%–60%), while lower tolerance was observed in acetonitrile and dimethylformamide (thresholds of 20% and 10%, respectively). Results of antibody activity retention rate and spectral analysis showed that methanol induced minimal structural and functional damage to OTA nanobodies; acetone severely impaired the nanobodies' structure and activity with significant destruction of their core domain; acetonitrile might affect antibody activity and tolerance via a non-structural mechanism; dimethylformamide exerted a drastic conformational impact, leading to complete structural disorder, acute activity loss, and the poorest tolerance. Molecular docking results indicated that organic solvents primarily interacted with framework residues via hydrogen bonds, without occupying the core antigen-binding region. This study elucidates the tolerance mechanism of the OTA nanobody in organic solvents, providing a theoretical basis for its application in complex sample detection and the design of solvent-resistant mutants.
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PubMed
高影响因子
Advanced Sustainable Systems
·
2026
·Vol. 10
·期 6
·e70537
·
IF 5.6
Pei Jia,Liangliang Peng,Rui Li,Sihua Hu,Jinbo Cao,Fusheng Zhong,Xingyan Lin,Qingqing Deng,Li Wang
Ochratoxin A (OTA) is very difficult to detect by conventional fluorescent technique directly due to its natural fluorescence is very weak. For the first time, it was found that in the presence of HPO 4 2− , the fluorescence intensity of OTA could be dramatically enhanced by more than 30-fold within the range of 0.025–0.45 µM. Based on this unique ability of HPO 4 2− , a novel fluorescent sensing platform for OTA was developed, which exhibited good sensitivity and high selectivity. The detection range for OTA was from 0.025 to 0.45 µM with a detection limit of 7.3 nM. The proposed method is direct, simple, rapid, sensitive, selective, and does not require any sophisticated procedures. Furthermore, this strategy can be extended to the fluorescent colorimetric detection of OTA. This study successfully established a robust, user-friendly analytical method, effectively addressing a key limitation in the direct fluorescent detection of OTA.
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaokun Wang,Hao Ding,Yinghui Sun,Yanling Ma,Guoqing Wang,Jiadong Chen,Jaebum Choo,Lingxin Chen
To address the need for highly sensitive and reliable detection of trace ochratoxin A (OTA) in food matrices, we developed a ratiometric fluorescent aptasensor by integrating CRISPR/Cas12a, hybridization chain reaction (HCR), and horseradish peroxidase (HRP)-induced inner filter effect (IFE). The mechanism involves OTA releasing an activator that initiates CRISPR/Cas12a trans-cleavage, blocking HCR assembly. This reduces HRP levels, limiting the conversion of o -phenylenediamine (OPD) to fluorescent 2,3-diaminophenazine (DAP) (emitting at 562 nm) while maintaining strong emission from 2-amino terephthalic acid (BDC-NH 2 ) at 426 nm. The F 426 /F 562 ratio serves as a “signal-on” indicator, enabling sensitive OTA detection over 0.1 pM to 10 nM, with a detection limit of 0.0417 pM. The method exhibits excellent reproducibility, with intra-day and inter-day relative standard deviations (RSDs) of 1.91 %–3.87 % and 1.79 %, respectively, along with recovery rates of 90.1 %–110.6 % in real samples. These advantages highlight its significant potential for CRISPR/Cas-based OTA detection.
研究领域:
食品安全
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分子生物学
·
分析化学
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DOI
PubMed
高影响因子
Journal of Biophotonics
·
2016
·Vol. 10
·期 5
·657-663
·
IF 3.818
Jingjing Yao,Guangxu Xing,Junling Han,Yaning Sun,Fangyu Wang,Ruiguang Deng,Xiaofei Hu,Gaiping Zhang
Novel direct and indirect competitive fluorescence-linked immunosorbent assays ( c FLISA and ic FLISA) for detection of ochratoxin A (OTA) were described using CdTe quantum dots (QDs) as fluorescent label. CdTe QDs were successfully synthesized, which had an emission wavelength of 615 nm. The high purity monoclonal antibody against OTA was prepared through cell thawing and the octylic acid-ammonium sulfate method. The OTA MAbs were successfully coupled with CdTe QDs, and which also retained the original biological activity. The 50% inhibition values (IC 50 ) of the c FLISA and ic FLISA were 0.630 ng/mL, 0.234 ng/mL, the limits of detection (LOD) were 7.06 × 10 –3 and 4.15 × 10 –3 ng/mL, and detection ranges were 7.06 × 10 –3 − 18.34 ng/mL and 4.15 × 10 –3 − 4.88 ng/mL, in-order. The recoveries were 96.0–104.7% along with coefficients of variation (CVs) below 10%. The FLISA provided novel method for determination of OTA and the potential of MAb–CdTe QDs for the establishment of fluorescent immunochromatographic test strip.
研究领域:
分析化学
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食品安全
·
毒理学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2026
·
IF 3.1
Lin-Lin Hu,Yang Yi,Xing-He Ke,Min-Min Ou,Lan-Lan Nong,Li-Zhou Qin,Shao-Chen Sun,Caizhu Wang,Bi-Yun Liao
IntroductionOchratoxin A is a toxic mycotoxin produced by fungi, which is commonly detected in contaminated cereals and animal feeds. It exhibits nephrotoxicity, hepatotoxicity, genotoxicity, immunotoxicity, and reproductive toxicity.MethodsIn the present study, we investigated the adverse effects of ochratoxin A on organelle distribution using a porcine oocyte model.ResultsWe demonstrated that in vitro exposure to ochratoxin A inhibited cumulus cell expansion and polar body extrusion in oocytes. Ochratoxin A reduced cortical endoplasmic reticulum distribution and increased GRP78 expression, indicating the induction of endoplasmic reticulum stress. In contrast, the Golgi apparatus exhibited abnormal accumulation, accompanied by decreased Rab7 expression and aberrant localization, suggesting impaired vesicular transport. Similarly, lysosomes accumulated in the cytoplasm of porcine oocytes following ochratoxin A treatment. We also found that mitochondrial function was disrupted, as evidenced by reduced ATP production and aberrant mitochondrial membrane potential.ConclusionTaken together, these results indicate that ochratoxin A impairs organelle distribution, induces endoplasmic reticulum stress, and causes vesicular transport defects during porcine oocyte maturation.
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高影响因子
TALANTA
·
2022
·
IF 6.556
Zefeng Mao,Xiaojuan Wang,Ruipeng Chen,Zixuan Zhou,Shuyue Ren,Jun Liang,Zhixian Gao
The CRISPR-Cas system has achieved breakthrough applications in the field of molecular diagnostics. CRISPR/Cas12a can accurately identify subtle changes in a target nucleic acid sequence and has a wide range of applications such as in highly sensitive detection methods. In this study, an upconversion-magnetic probe-DNA-Fe 3 O 4 probe was designed to replace traditional fluorescent probes using nucleic acid aptamers to design a biosensing method powered by CRISPR/Cas12a. The CRISPR/Cas12a technology can be widely used for sensitive, rapid, and stable detection of ochratoxin A. In our sensing strategy, the ochratoxin A capture probe was used to capture ochratoxin A and release the Cas protein activation chain, further triggering the single-stranded DNA degradation activity of CRISPR/Cas12a and removal of the fluorescent probe to generate a fluorescent signal. Compared with traditional commercial kits, our method was more rapid and exhibited comparable detection capability.
研究领域:
分子生物学
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食品安全检测
·
分析化学
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DOI
PubMed
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2022
·Vol. 86
·期 9
·1192-1199
·
IF 2.337
Wu Jinhai,Zhao Jiamei,Liu Mingzhu,Zhao Zunquan,Qiu Yu,Li Hanle,Wu Jin,Bai Jialei
We developed a new ochratoxin A (OTA) aptamer biosensor to promptly detect OTA in food. Mesoporous silica nanoparticles were used as carriers, and aptamers were used as recognition probes and gating molecules. The fluorescent dye rhodamine 6G was loaded into mesoporous silica, and through electrostatic contact, the OTA aptamer was adsorbed on amino-modified mesoporous silica. The fluorescent dye released from the mesopore in the presence of OTA because of the conformational change induced in the aptamer by the target. The amount of ochratoxin was determined by measuring the fluorescence intensity. Our findings revealed a positive relationship between the fluorescence intensity and OTA concentration, with a limit of detection of 0.28 ng mL−1, and the detection range was 0.05–200 ng mL−1. The recovery rate was 80.7%–110.8% in real samples. The proposed approach is suitable for the quantification of other toxins.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 11
·955
·
IF 5.4
Xiujin Chen,Dong Gao,Jiaqi Chen,Xueqing Wang,Chifang Peng,Hongli Gao,Yao Wang,Zhaozhou Li,Huawei Niu
Sensitive detection of ochratoxin A (OTA) is significant and essential because OTA may pose risks to human and animal health. Here, we developed an electrochemical aptasensor for OTA analysis using polyamidoamine (PAMAM) dendrimers as a signal amplifier. As a carrier, PAMAM has numerous primary amino groups that can be coupled with thiolated complementary strand DNA (cDNA), allowing it to recognize aptamers bound to the surface of horseradish peroxidase (HRP)-modified gold nanoparticles (AuNPs), thereby improving the sensitivity of the aptasensor. When monitoring the positive samples, OTA was captured by the aptamer fixed on the HRP-conjugated AuNP surface by specific recognition, after which the formed OTA-aptamer conjugates were detached from the electrode surface, ultimately decreasing the electrochemical signal monitored by differential pulse voltammetry. The novel aptasensor achieved a broad linear detection range from 5 to 105ng L−1with a low detection limit of 0.31 ng L−1. The proposed aptasensor was successfully applied for OTA analysis in red wine, with recovery rates ranging from 94.15 to 106%. Furthermore, the aptasensor also exhibited good specificity and storage stability. Therefore, the devised aptasensor represents a sensitive, practical and reliable tool for monitoring OTA in agricultural products, which can also be adapted to other mycotoxins.Keywords:Ochratoxin A;PAMAM;electrochemical aptasensor;graphene oxide;AuNPs
研究领域:
分析化学
·
食品安全
·
纳米技术
·
毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）