DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
·
2019
·
IF 3.119
Liting Wu,Enxu Zhou,Along Gao,Linghe Kong,Siwei Wu,Xia Bian,Yuan Li,Bingxi Li,Shengli Fu,Zheng Guo,Jianmin Ye
B lymphocyte-induced maturation protein 1 (Blimp-1), a transcription factor containing zinc finger , is required and sufficient to trigger terminal differentiation of B cells in mammals. The Blimp-1 ( OnBlimp-1 ) from Nile tilapia ( Oreochromis niloticus ) was identified and characterized its expression pattern during B cell activation and maturation. The cDNA of OnBlimp-1 open reading frame is 2547 bp encoding a protein of 848 amino acids and the predicted molecular weight is 93.36 kDa. OnBlimp-1 contains a SET domain and five ZnF_C2H2 domains, which shares high homology with that of other species. OnBlimp-1 transcription was detected in all examined tissues with high expression in the spleen (SPL). Analysis of sorted lymphocyte populations , including IgM + and IgM − cells from peripheral blood (PBL), SPL and anterior kidney (AK), indicated that the OnBlimp-1 transcription was highly expressed in the IgM + B cells. Upon LPS stimulation, OnBlimp-1 expression was up-regulated in tissues of PBL, SPL and AK significantly. The expression of OnBlimp-1 , as well as the secreted IgM , was significantly up-regulated in the SPL and AK leukocytes stimulated with anti-OnIgM monoclonal antibody and LPS in vitro , respectively. Above results suggest that OnBlimp-1, a cytokine regulating the terminal differentiation of activated B cells to antibody-secreting cells, is likely to play important roles in B cell activation and maturation in Nile tilapia.
研究领域:
免疫学
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分子生物学
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鱼类生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MARINE ENVIRONMENTAL RESEARCH
·
2022
·
IF 3.737
Shaoying Xing,Ping Li,Shuwen He,Zhihan Cao,Xu Wang,Xuqian Cao,Bin Liu,Chengzhuang Chen,Hong You,Zhi-Hua Li
Triphenyltin (TPT) has attracted considerable attention owing to its vitality, bioaccumulation , and lurking damage. TPT widely exists in complex salinity areas such as estuaries and coastal regions. However, there are few studies on the toxicological behavior of TPT under different salinity. In the study, juvenile Nile tilapia ( Oreochromis niloticus ) were utilized as model animals to investigate the effects of environmental relevant TPT exposure on the osmoregulation and energy metabolism in gill under different salinity. The results showed that salinity and TPT single or combined exposure affected the morphology of the gill tissue. After TPT exposure, Na + -K + -ATPase (NKA) activity significantly decreased at 0 ppt, while NKA and Ca 2+ -Mg 2+ -ATPase (CMA) activities significantly increased at 15 ppt. In addition, significantly higher succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) activities were found in the control fish compared to the TPT-exposed ones at 15 ppt. Quantitative real-time PCR results showed that TPT exposure affected the expression of osmoregulation and energy metabolism-related genes under different salinity. Overall, TPT exposure interfered with osmoregulation and energy metabolism under different salinity. The study will provide reference data for assessing the toxicity of organotin compounds in complex-salinity areas.
研究领域:
水生毒理学
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环境科学
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鱼类生理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FISH BIOLOGY
·
2022
·Vol. 101
·期 3
·431-440
·
IF 2.504
Along Gao,Liting Wu,Lan Li,Biao Han,Jianmin Ye
The CXC chemokine receptors (CXCRs) are members of the seven transmembrane (7-TM) G-protein-coupled receptor superfamily that involves innate and adaptive immune systems. In this study, CXCR3a and CXCR3b from Nile tilapia ( Oreochromis niloticus ) were cloned and identified, designated as OnCXCR3a and OnCXCR3b . The open reading frames of OnCXCR3a and OnCXCR3b were 1074 and 1080 bp, encoding the predicted proteins of 357 and 359 amino acids, respectively. Multiple alignment analysis of OnCXCR3a- and OnCXCR3b-deduced protein sequences with the mammalian and bird sequences indicated the presence of typical structural features of chemokine receptors, including a 7-TM domain and conserved motifs. Quantitative real-time PCR analysis revealed that OnCXCR3a and OnCXCR3b were constitutively expressed in a wide range of tissues. When stimulated with Streptococcus agalactiae , Aeromonas hydrophila , polyinosinic:polycytidylic acid and lipopolysaccharide in vivo or in vitro on leukocytes, the mRNA levels of OnCXCR3a and OnCXCR3b were significantly upregulated. Overall, these results indicated that OnCXCR3s might be involved in host immune responses in Nile tilapia.
研究领域:
免疫学
·
分子生物学
·
鱼类生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2019
·
IF 3.298
Liting Wu,Yanjian Yang,Linghe Kong,Xia Bian,Zheng Guo,Shengli Fu,Fang Liang,Bingxi Li,Jianmin Ye
In teleost fish, IgM + B cells play important roles in innate and adaptive immunity. Different IgM + B cells are detected in teleost, named IgM lo and IgM hi B cell subsets , according to the distinct expression levels of membrane IgM (mIgM). However, the study on the heterogeneity in IgM + B cell subsets remains poorly understood. In this study, the comparative transcriptomic profiles of IgM − , IgM lo and IgM hi from peripheral blood of Nile tilapia ( Oreochromis niloticus ) were carried out by using RNA-sequencing technique. A total of 6045 and 5470 differentially expressed genes (DEGs) were detected in IgM lo and IgM hi cells, respectively, as compared with IgM − lymphocytes, whereas 3835 genes were differentially expressed when IgM lo compared to IgM hi cells. Analysis of the KEGG database indicated that the DEGs were enriched in immune system categories and signaling transduction and interaction in IgM − vs IgM hi , IgM − vs IgM lo and IgM lo vs IgM hi . Comparatively, in IgM lo vs IgM hi , GO enrichment analysis indicated that the DEGs enriched in nucleic acid binding transcription factor activity. Analysis of crucial transcription factors for B cell differentiation indicated that IgM lo and IgM hi cell clusters belonged to the different B cell subsets. The data generated in this study may provide insights into understanding the heterogeneity of IgM + cells in teleost, and suggest that IgM + B cells play a crucial role in innate immunity .
研究领域:
免疫学
·
转录组学
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鱼类生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2020
·
IF 0
Liting Wu,Along Gao,Yang Lei,Jun Li,Kangsen Mai,Jianmin Ye
Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP1), a kind of protein tyrosine phosphatases (PTPs), is a critical regulator of antigen receptor signal transduction . Signal transduction of BCR is regulated by phosphatases in teleost as in mammals. In this study, SHP1 from Nile tilapia ( Oreochromis niloticus ) (OnSHP1) was identified and characterized, including the expression pattern against bacterial infection and regulation function in BCR signaling pathway. The open reading frame of OnSHP1 contains 1749 bp of nucleotide sequence , encoding a protein of 582 amino acids. The OnSHP1 protein was highly conversed compared to that of other species, including two amino-terminal SH2 domains at the N terminus and a PTP catalytic domain . Transcriptional expression analysis revealed that OnSHP1 was detected in all examined tissues and highly expressed in spleen . The up-regulated OnSHP1 expression was observed in peripheral blood, spleen and anterior kidney following challenge with Streptococcus agalactiae or lipopolysaccharide (LPS) in vivo , as well as that displayed in leukocytes stimulated with S. agalactiae or LPS in vitro . Further, after induction with mouse anti-tilapia IgM monoclonal antibody in vitro , OnSHP1 was significantly up-regulated in leukocytes. When spleen leukocytes treated with PTP Inhibitor II in vitro , the phosphorylation level of OnSHP1 at the phosphorylation sites (Y 535 and Y 557 ) and the cytoplasmic free Ca 2+ concentration were up-regulated significantly. Overall, the findings of this study indicate that SHP1 gets involved in host defense against bacterial infection and BCR signaling pathway in Nile tilapia .
研究领域:
免疫学
·
分子生物学
·
鱼类生物学
·
病原-宿主互作
✓ 已验证
产品关联
DOI
PubMed
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Xing Li,Baijian Jiang,Zhiqiang Zhang,Meiling Huang,Jiamin Feng,Yongxiong Huang,Kwaku Amoah,Yu Huang,Jichang Jian
Interleukin 8 ( IL8 ) is vital in promoting inflammation and is a crucial mediator in various physiopathological processes while influencing immunological function. The effect of IL8 on the immunological response to acute bacterial infections in Nile tilapia ( Oreochromis niloticus) remains unknown. This work found an IL8 gene from Nile tilapia ( On-IL8 ). It includes a 285 bp open reading frame and codes for 94 amino acids. The transcript levels of On-IL8 were highest in the head-kidney tissue and sharply induced by Streptococcus agalactiae and Aeromonas hydrophila . Besides, in vitro experiments revealed that On-IL8 regulated a variety of immunological processes and promoted inflammatory responses. Moreover, On-IL8 suppressed the NF-κB signaling pathway , consistent with in vitro results. These significant findings serve as the basis for further investigation into how IL8 confers protection to bony fish in opposition to bacterial infections.
研究领域:
免疫学
·
水产养殖
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2017
·
IF 3.148
Liangliang Mu,Xiaoxue Yin,Jing Liu,Liting Wu,Xia Bian,Yuhong Wang,Jianmin Ye
Mannose-binding lectin (MBL) is a pattern recognition protein that plays an important role in innate immunity capable of activating the lectin pathway of the complement system. In this study, a MBL homologue (OnMBL) was identified from Nile tilapia ( Oreochromis niloticus ) and characterized at expression and agglutination functional levels. The open reading frame of OnMBL is 687 bp of nucleotide sequence encoding polypeptides of 228 amino acids. The deduced amino acid sequence is highly homology to teleost and similar to mammalian MBL, containing a canonical collagen-like region, a carbohydrate recognition domain and a neck region. Expression analysis revealed that the OnMBL was highly expressed in the liver, and also exhibited in other tissues including hind kidney , intestines, head kidney and spleen. In addition, the OnMBL expression was significantly up-regulated in spleen and head kidney following challenges with a Gram-positive bacterial pathogen ( Streptococcus agalactiae ) and a Gram-negative bacterial pathogen ( Aeromonas hydrophila ). Recombinant OnMBL ((r)OnMBL) protein was able to agglutinate both S. agalactiae and A. Hydrophila in vitro. Taken together, the results of this study indicated that OnMBL, possessing apparent agglutination ability to bacterial pathogens, might be involved in host defense against bacterial infection in Nile tilapia.
研究领域:
免疫学
·
分子生物学
·
鱼类病理学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE
·
2018
·
IF 2.71
Shuo Liu,Meng Chen,Fangfang Yan,Enxu Zhou,Bingxi Li,Shengli Fu,Xiaoxue Yin,Zheng Guo,Jianmin Ye
Polymeric immunoglobulin receptor (pIgR) plays important roles in transport of polymeric immunoglobulins across mucosal epithelial cells and prevention of access of pathogens into the organisms. In this study, a pIgR ortholog ( OnpIgR ) was cloned and identified from Nile tilapia ( Oreochromis niloticus ). The open reading frame (ORF) of OnpIgR is 1023 bp, encoding a predicted protein of 341 amino acids. The deduced amino acid sequence of OnpIgR is highly homologous to other teleost's pIgRs, containing two Ig-like domains (ILDs), a transmembrane region and an intracellular region. Expression analysis revealed that OnpIgR was exhibited in all tested tissues including liver, spleen, skin and intestines, and highly expressed in the liver. Immunofluorescence detection indicated that the natural pIgR expressed on the membrane of the epithelial cells. After challenge of bacterial pathogen ( Streptococcus agalactiae ), OnpIgR expression was significantly up-regulated in intestines and skin, as well as intestinal epithelial cells. In addition, recombinant OnIFN-γ induced the expression of pIgR in the intestinal epithelial cells. Moreover, recombinant OnpIgR protein was able to bind both S. agalactiae and A. hydrophila in vitro , and interact with (r)OnIgM and (r)OnIgT. Taken together, the results indicated that OnpIgR, involving in the polymeric immunoglobulin transport, might play an important role in mucosal immunity against bacterial infection.
研究领域:
免疫学
·
水产养殖
·
分子生物学
✓ 已验证
DOI
高影响因子
Food Science and Human Wellness
·
2022
·
IF 8.022
Jun Yang,Mingyue Shen,Ting Wu,Xianxiang Chen,Huiliang Wen,Jianhua Xie
In this study, chondroitin sulfate was extracted from Oreochromis niloticus bones (OCS) and isolated to three fractions (OCS-1, OCS-2, and OCS-3). The physicochemical properties and structure characterization including monosaccharide, disaccharide compositions, molecular weight ( Mw ) of OCS were determined by HPAEC, HPLC-SAX, HPGPC, FT-IR spectra, and 1D/2D NMR. Moreover, their thermal properties, crystalline structure, and microstructure were also analyzed. Results showed that their Mw were between 10 kDa and 50 kDa. CS-6 was the predominant disaccharide unit in four OCS, and the CS-4/CS-6 ratios were close to CS from shark cartilage. Besides, the results of antioxidant activity showed that different fractions of OCS had a distinct DPPH radical, hydroxyl radical, and ABTS + radical scavenging activity. OCS-1 has the highest scavenging activities in DPPH and hydroxyl radical compared with other fractions, which showed a higher medicinal value. Those findings may lay some theoretical basis for the potential application development of OCS.
研究领域:
生物化学
·
食品科学
·
药学
✓ 已验证
DOI
高影响因子
Frontiers in Immunology
·
2021
·
IF 7.561
Wu Liting,Gao Along,Li Lan,Chen Jianlin,Li Jun,Ye Jianmin
Teleost fish anterior kidney (AK) is an important hematopoietic organ with multifarious immune cells, which have immune functions comparable to mammalian bone marrow. Myeloid and lymphoid cells locate in the AK, but the lack of useful specific gene markers and antibody-based reagents for the cell subsets makes the identification of the different cell types difficult. Single-cell transcriptome sequencing enables single-cell capture and individual library construction, making the study on the immune cell heterogeneity of teleost fish AK possible. In this study, we examined the transcriptional patterns of 11,388 AK leukocytes using 10× Genomics single-cell RNA sequencing (scRNA-seq). A total of 22 clusters corresponding to five distinct immune cell subsets were identified, which included B cells, T cells, granulocytes, macrophages, and dendritic cells (DCs). However, the subsets of myeloid cells (granulocytes, macrophages, and DCs) were not identified in more detail according to the known specific markers, even though significant differences existed among the clusters. Thereafter, we highlighted the B-cell subsets and identified them as pro/pre B cells, immature/mature B cells, activated B/plasmablasts, or plasma cells based on the different expressions of the transcription factors (TFs) and cytokines. Clustering of the differentially modulated genes by pseudo-temporal trajectory analysis of the B-cell subsets showed the distinct kinetics of the responses of TFs to cell conversion. Moreover, we classified the T cells and discovered that CD3+CD4−CD8−, CD3+CD4+CD8+, CD4+CD8−, and CD4−CD8+ T cells existed in AK, but neither CD4+CD8− nor CD4−CD8+ T cells can be further classified into subsets based on the known TFs and cytokines. Pseudotemporal analysis demonstrated that CD4+CD8− and CD4−CD8+ T cells belonged to different states with various TFs that might control their differentiation. The data obtained above provide a valuable and detailed resource for uncovering the leukocyte subsets in Nile tilapia AK, as well as more potential markers for identifying the myeloid and lymphoid cell types.
研究领域:
免疫学
·
鱼类生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE
·
2020
·
IF 3.225
Xiaoxue Yin,Hairong Wu,Liangliang Mu,Kailiang Han,Heyi Xu,Jichang Jian,Anli Wang,Jianmin Ye
Calreticulin (CRT) as a multifunctional Ca 2+ -binding protein is highly conserved in variety species. Recently, the structure and function analysis of CRT in teleost has attracted wide attention. The present study first amplified the open reading frame of CRT mRNA from Nile tilapia ( Oreochromis niloticus ), then characterized its tissue distribution and expression pattern against the challenge of pathogenic bacteria. Furthermore, analyzed the function of OnCRT in regulating phagocytosis and inflammatory response by monocytes/macrophages (MO/Mø). The results showed that the ORF of OnCRT is 1269 bp of nucleotide and encoding a polypeptide with 422 amino acids. OnCRT protein is highly homologous to the CRT in other species, containing three conserved structure domain, the N-domain, P-domain and C domain. The OnCRT mRNA was widely exhibited in the collected tissues of natural tilapia, and with the maximum in the liver. Following the challenges of Streptococcus agalactiae and Aeromonas hydrophila in vivo and in vitro , the expression of OnCRT transcripts was significantly upregulated. The recombinant OnCRT protein was purified with the His Band Resin columns, and confirmed to have the capacity of binding with bacteria and promoted the phagocytosis of MO/Mø. In addition, the expressions of inflammatory factors and chemokines in MO/Mø were increased significantly upon the stimulation of (r)OnCRT. In summary, the present study indicated that OnCRT was likely to get involved in host defense against bacterial infection in O. niloticus .
研究领域:
免疫学
·
分子生物学
✓ 已验证
DOI
高影响因子
Frontiers in Nutrition
·
2023
·
IF 5
Yucheng Hou,Xian’e Ren,Yongchun Huang,Kun Xie,Keyao Wang,Liyang Wang,Fengyan Wei,Feng Yang
The purpose of this research was to explore the different hydrodynamic cavitation (HC) times (0, 5, 10, 15, 20 min; power 550 W, pressure 0.14 MPa) on the emulsifying properties of tilapia myofibrillar protein (TMP). Results of pH, particle size, turbidity, solubility, surface hydrophobicity, and reactive sulfhydryl (SH) group indicated that HC changed the structure of TMP, as confirmed by the findings of intrinsic fluorescence and circular dichroism (CD) spectra. Furthermore, HC increased the emulsifying activity index (EAI) significantly ( P < 0.05) and changed the emulsifying stability index (ESI), droplet size, and rheology of TMP emulsions. Notably, compared with control group, the 10-min HC significantly decreased particle size and turbidity but increased solubility ( P < 0.05), resulting in accelerated diffusion of TMP in the emulsion. The prepared TMP emulsion showed the highest ESI (from 71.28 ± 5.50 to 91.73 ± 5.56 min), the smallest droplet size (from 2,754 ± 110 to 2,138 ± 182 nm) and the best rheological properties, as demonstrated by the microstructure photographs. Overall, by showing the effect of HC in improving the emulsifying properties of TMP, the study demonstrated HC as a potential technique for meat protein processing.
研究领域:
食品科学
·
蛋白质化学
·
生物物理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）