Journal of Advanced Research
·
2020
·
IF 6.992
Yuanze Zhou,Lijing Liao,Xikai Liu,Biao Liu,Xinxin Chen,Yan Guo,Chuanlong Huang,Yucheng Zhao,Zhixiong Zeng
Plant tryptophan decarboxylase (TDC) is a type II Pyridoxal-5′-phosphate-dependent decarboxylase (PLP_DC) that could be used as a target to genetically improve crops. However, lack of accurate structural information on plant TDC hampers the understanding of its decarboxylation mechanisms. In the present study, the crystal structures of Oryza sativa TDC ( O sTDC) in its complexes with pyridoxal-5′-phosphate, tryptamine and serotonin were determined. The structures provide detailed interaction information between TDC and its substrates. The Y359 residue from the loop gate is a proton donor and forms a Lewis acid-base pair with serotonin/tryptamine, which is associated with product release. The H214 residue is responsible for PLP binding and proton transfer, and its proper interaction with Y359 is essential for Os TDC enzyme activity. The extra hydrogen bonds formed between the 5-hydroxyl group of serotonin and the backbone carboxyl groups of F104 and P105 explain the discrepancy between the catalytic activity of TDC in tryptophan and in 5-hydroxytryptophan. In addition, an evolutionary analysis revealed that type II PLP_DC originated from glutamic acid decarboxylase, potentially as an adaptive evolution of mechanism in organisms in extreme environments. This study is, to our knowledge, the first to present a detailed analysis of the crystal structure of Os TDC in these complexes. The information regarding the catalytic mechanism described here could facilitate the development of protocols to regulate melatonin levels and thereby contribute to crop improvement efforts to improve food security worldwide.
研究领域:
植物学
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生物化学
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遗传学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 40
·91997-92006
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IF 5.8
Ran
Chunmei,Liu Yubin,Li Ke,Wang Chenye,Pu Jian,Sun Hongwen,Wang Lei
Although the combined effect of organic ligands and heavy metals in the environment on plants have been frequently reported, their complexed interaction in plants and the physiological effects remain to be revealed. Metal complexing agent benzotriazole (BTR) has extensive environmental pollution. In this study, root-splitting experiments were designed to identify the in vivo and in vitro effects of BTR on the accumulation and translocation of Cu in rice ( Oryza sativa L . ), and the concentrations and translocation factor (TF) of Cu and BTR in different parts of rice were measured. In the in vitro interaction treatments, low BTR concentrations enhanced Cu uptake and lateral transport in rice, while higher levels of BTR’s exposure (i.e., ≥ 100 μM) resulted in opposite effects. Differently, significant increase in the lateral transport of Cu and vertical translocation of BTR in rice presented in the in vivo interaction treatments. TF of Cu from root A to root B (TF RA-RB ) increased from 0.05 to 0.272 with the BTR concentration increasing from 0 to 100 μM, and higher TF of BTR from root to shoot (TF R-S ), ranging from 1.00 to 1.75, compared with single BTR exposure treatments was observed. The phytotoxicity of BTR expressed by the catalase activity was significantly alleviated by the in vivo accumulated Cu in rice.
研究领域:
环境科学
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植物生理学
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毒理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
·
2022
·
IF 6.028
Wenxiao Zhang,Haiou Wang,Qian Bai,Xintong Li
To understand uptake and accumulation process of organophosphate esters (OPEs), growth, concentrations of OPEs, and transcript regulation of lipid transporters in rice seedlings were investigated under six OPE congeners exposures. The increase of seedlings’ growth and accumulation of OPEs in plants were observed. Root concentration factors (RCFs) of aryl-OPEs were the highest in consistent with their strong hydrophobicity, indicating that they were more liable to be accumulated in roots. Meanwhile, shoot/root concentration factors (SRCFs) of halogenated alkyl-OPEs were the highest in accordance with their low hydrophobicity, implying that they were easily translocated to shoots. Lipid transporters involved in uptake and accumulation of OPEs, evidence from the transcript responses of their lipid transporters. Moreover, the only positive relationship between OsTIL expressions and accumulation of OPEs demonstrated the dominant role of OsTIL among the investigated transporters. In addition, although all of OPE congeners could be docked into active cavities of OsTIL with varied markedly binding affinities, hydrophobicity OPEs performed stronger binding energy with OsTIL after homology modeling and molecular docking analysis. Therefore, uptake and accumulation of OPEs were corporately charged by OPEs’ physicochemical properties and lipid carrier transports in coincidence with the experimental observation about accumulation of OPEs in roots and shoots.
研究领域:
环境科学
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植物生理学
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分子生物学
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产品关联
DOI
高影响因子
Journal of Chemistry
·
2019
·
IF 1.727
Yuchao Feng,TianXin Fu,Liyuan Zhang,Changyuan Wang,Dongjie Zhang
The analytical method for the metabolomics of the 60 rice seeds from two main rice origins in Heilongjiang Province was developed based on gas chromatography coupled with mass spectrum. The specific differential metabolites between two rice origins were identified, and the distinguish of the two main origins was illustrated by using the R software platform with XCMS software package for gas chromatography coupled with mass spectrum data processing, combined with multivariate statistical analysis software. The result indicated that the 173 peaks were detected, and 54 of which were structurally identified, covering amino acids, aliphatic acid, sugar, polyols, and so on. By comparing the data of Wuchang and Jiansanjiang origins, it was found that there were 9 special metabolites in Wuchang origin and 8 special metabolites in Jiansanjiang origin. The 10 differential metabolites with significant changes (, VIP ≥ 1) were filtrated. It is indicated that the differential metabolites of rice carry information of their origin and there are the differences in the metabolites of rice in two main origins. The proposed method is expected to be useful for the metabolomic researches of rice.
研究领域:
农业科学
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植物科学
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分析化学
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代谢组学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yi Hu,Nuzahat Habibul,Yan-Yun Hu,Fan-Li Meng,Guo-Ping Sheng
Ciprofloxacin (CIP), a widely used fluoroquinolone antibiotic, is frequently detected in aqueous environments, and could be assimilated by vegetable plants to possess potential threats to human and animal health through food chains. However, plant uptake of CIP in different chemical speciation has still far from clear now. Thus, the toxicity and uptake of CIP by rice plants were investigated under different solution pH, owing to its contribution to different chemical speciation of CIP. Results display that high pH-driven changes of CIP from cation (CIP + ) to anion (CIP − ) decreased its adsorption and uptake by excised roots and intact plants, respectively. However, CIP concentrations in roots, stems and leaves all exhibited no significant differences with increasing solution pH. Moreover, six intermediates of CIP were detected and two possible transformation pathways were proposed in rice plants, including firstly oxidation and following consecutive cleavage of piperazine ring. After accumulated in plant tissues, CIP significantly inhibited the plant growth, decreased the photosynthetic pigments contents and enhanced the antioxidant enzyme activities in a concentration-dependent manner. Besides, high pH exacerbated the growth inhibition and changed the oxidative damage responses of rice plants to CIP. These findings indicate that the uptake and toxicity of CIP in rice plants were influenced by solution pH-driven changes of its chemical speciation.
研究领域:
环境科学
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植物科学
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毒理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shifeng Tang,Lin Zhang,Zhaohui Tong,Zhiping Wu,Hui Wang,Peng Zhan,Lishu Shao,Yan Qing,Yougen Wu,Jin Liu
As an essential trace element for plant growth and development , manganese plays a crucial role in the uptake of the heavy metal cadmium by rice ( Oryza sativa L.). In this study, we developed a novel slow-release manganese fertilizer named Mn@LNS-EL. Initially, lignin nanoparticles were derived from sodium lignosulfonate, and a one-step emulsification strategy was employed to prepare a water-in-oil-in-water (W/O/W) Pickering double emulsions. These double emulsions served as the template for interfacial polymerization of lignin nanoparticles and epichlorohydrin, resulting in the formation of microcapsule wall materials. Subsequently, manganese fertilizer (MnSO 4 ) was successfully encapsulated within the microcapsules. Hydroponic experiments were conducted to investigate the effects of Mn@LNS-EL on rice growth and the cadmium and manganese contents in the roots and shoots of rice under cadmium stress conditions. The results revealed that the treatment with Mn@LNS-EL markedly alleviated the inhibitory effects of cadmium on rice growth, leading to notably lower cadmium levels in the rice roots and shoots compared to the specimens treated without manganese fertilizer. Specifically, there was a reduction of 37.9 % in the root cadmium content and a 17.1 % decrease in the shoot cadmium content. In conclusion, this study presents an innovative approach for the high-value utilization of lignin through effective encapsulation and slow-release mechanisms of trace-element fertilizers while offering a promising strategy for efficiently remediating cadmium pollution in rice.
研究领域:
农业科学
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环境科学
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材料科学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
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IF 8.8
Hanxin Wu,Xiaohan Jiang,Jianhao Tong,Jing Wang,Jiyan Shi
Nowadays, Lead (Pb) and Cadmium (Cd) contamination in rice is an important worldwide environmental concern. Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) and Nano hydroxyapatite (n-HAP) are promising materials to manage Pb and Cd contamination. This study systematically investigated the effect of Fe 3 O 4 NPs and n-HAP on Pb and Cd stressed rice seedlings’ growth, oxidative stress , Pb and Cd uptake and subcellular distribution in roots. Furthermore, we clarified the immobilization mechanism of Pb and Cd in the hydroponic system. Fe 3 O 4 NPs and n-HAP could reduce Pb and Cd uptake of rice mainly through decreasing Pb and Cd concentrations in culture solution and combining with Pb and Cd in root tissues. Pb and Cd were immobilized by Fe 3 O 4 NPs through complex sorption processes and by n-HAP through dissolution-precipitation and cation exchange, respectively. On the 7th day, 1000 mg/L Fe 3 O 4 NPs reduced the contents of Pb and Cd in shoots by 90.4% and 95.8%, in roots by 23.6% and 12.6%, 2000 mg/L n-HAP reduced the contents of Pb and Cd in shoots by 94.7% and 97.3%, in roots by 93.7% and 77.6%, respectively. Both NPs enhanced the growth of rice seedlings by alleviating oxidative stress and upregulating glutathione secretion and antioxidant enzymes activity . However, Cd uptake of rice was promoted at certain concentrations of NPs. The subcellular distribution of Pb and Cd in roots indicated that both NPs decreased the percentage of Pb and Cd in the cell wall, which was unfavorable for Pb and Cd immobilization in roots. Cautious choice was needed when using these NPs to manage rice Pb and Cd contamination.
研究领域:
环境科学
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农学
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纳米技术
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植物生理学
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2016
·Vol. 9
·期 12
·3501-3508
·
IF 2.167
Shao Yufang,Chen Ying,Liu Jian,Wu Pingping,Ying Yibin,Xie Jing
High-resolution inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine potential toxic element pollution in rice and paddy soil in a lead-acid battery production area. The contamination levels of chromium (Cr), cadmium (Cd), and lead (Pb) in rice and soil at three sampling areas were determined, and the uptake mechanism of Cr, Cd, and Pb from soil to rice grain was preliminarily analyzed. The results show that the range of potential toxic elements in rice concentrations is 3.914–11.788, 0.057–1.215, and 0.187–1.585 mg kg −1 for Cr, Cd, and Pb, respectively. For the paddy soils, Cd concentration is 0.610 mg kg −1 , which exceeds 0.300 mg kg −1 , a value recommended by Chinese Environmental Quality. Bioaccumulation factor (BAF, an index of the ability of plant to absorb a particular metal with respect to its concentration in the soil substrate) values of Cr, Cd, and Pb were less than 1 in all rice samples, suggesting that the bioavailability of these metals was relatively low in rice. The correlations between BAF values with organic matter (OM) and pH were calculated to find the mobility and availability of potential toxic elements in soil. At last, the estimated daily intake (EDI) dose was determined to evaluate the health risk to local population via consumption of rice contaminated with Cr, Cd, and Pb. This study indicated that the accumulation and translocation of potential toxic elements from soil to rice could cause the health risk for the local population.
研究领域:
环境科学
·
农业化学
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毒理学
·
公共卫生
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Huijun Liu,Mengjun Wang,Siyuan Jin,Qian Guo,Shuping Wang,Haohua Ni,Hainan Lu,Zhiheng Li
Massive use of plastic products has caused their accumulation in soils, releasing large amounts of endogenous plastic additives (e.g., benzotriazole ultraviolet stabilizers, in short BZT-UVs) into terrestrial ecosystems. However, their plant toxicity is little known. Herein, we investigated the occurrence of BZT-UVs in contaminated farmland and selected three BZT-UV congeners to explore their toxic effects on the antioxidant, photosynthetic, and metabolic perturbation on rice ( Oryza sativa ). Results showed that the mean concentrations of ∑BZT-UVs in soil and plant samples were 180.7 ng/g dw and 156.4 ng/g dw, respectively. UV-P, UV-327 and UV-328 were the dominant BZT-UV congeners in both of soils and plants. Three BZT-UV congeners caused oxidative damages to rice in a dose-dependent manner, especially for UV-328. Functional genes involved in chlorophyll synthetases was inhibited by over 50 % under the stress of BZT-UVs, whereas those responsible for chlorophyll degradation were obviously promoted. The chlorophyll content was thus decreased, leading to a weakened photosynthesis system and an unbalanced carbon metabolism. The transcriptome and metabolome proved that the flux of carbohydrate metabolism and amino acid metabolism were obviously promoted in plants induced by BZT-UVs, which could inhibit the growth of rice. These findings offered insights into the coordinated responses of plants and advanced our understanding of potential ecological risks of BZT-UVs to terrestrial ecosystems.
研究领域:
环境科学
·
植物生理学
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毒理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
NanoImpact
·
2024
·
IF 4.7
Nan Zhao,Chang Liu,Chenyang Ji,Xuefeng Jiang,Jinyu Zhao,Liwen Qiang,Hangbiao Jin
Titanium dioxide nanoparticles (TiO 2 NPs) have been widely used in agriculture, which increased the risk to soil-plant systems. Studies have demonstrated that TiO 2 NPs can induce phytotoxicity. However, the toxicity mechanisms, particularly under the stress of TiO 2 NPs with different crystalline forms, remain inadequately reported. In this study, we combined transcriptomics and metabolomics to analyze the toxicity mechanisms in rice ( Oryza sativa L.) under the stress of anatase (AT) or rutile (RT) TiO 2 NPs (50 mg/kg, 40 days). The length (decreased by 1.1-fold, p = 0.021) and malondialdehyde concentration (decreased by 1.4-fold, p = 0.0027) of rice shoots was significantly reduced after AT exposure, while no significant changes were observed following RT exposure. Antioxidant enzyme activities were significantly altered both in the AT and RT groups, indicating TiO 2 NPs induced rice oxidative damage (with changes of 1.1 to 1.4-fold, p < 0.05). Additionally, compared to the control, AT exposure altered 3247 differentially expressed genes (DEGs) and 56 significantly differentially metabolites in rice (collectively involved in pyrimidine metabolism, TCA cycle, fatty acid metabolism, and amino acid metabolism). After RT exposure, 2814 DEGs and 55 significantly differentially metabolites were identified, which were collectively involved in fatty acid metabolism and amino acid metabolism. Our results indicated that AT exposure led to more pronounced changes in biological responses related to oxidative stress and had more negative effects on rice growth compared to RT exposure. These findings provide new insights into the phytotoxic mechanisms of TiO 2 NPs with different crystalline forms. Based on the observed adverse effects, the study emphasizes that any form of TiO 2 NPs should be used with caution in rice ecosystems. This study is the first to demonstrate that AT is more toxic than RT in paddy ecosystems, providing crucial insights into the differential impacts and toxic mechanisms of TiO 2 NPs with different crystalline forms. These findings suggest prioritizing the use of RT when TiO 2 NPs are necessary in agricultural development to minimize toxicity risks.
研究领域:
农业
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环境科学
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植物生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Youming Dong,Qiongli Bao,Minling Gao,Weiwen Qiu,Zhengguo Song
Although the compound pollution of microplastics and arsenic (As) in paddy soil can affect the growth and quality of rice, relevant research on this phenomenon was limited. Therefore, we combined a pot experiment with computational chemistry to explore the effects and mechanism of polystyrene (PSMP) and polytetrafluoroethylene (PTFE) microplastics on As bioavailability. PSMP and PTFE interacted with rice root exudates through van der Waals forces, approached the rice root system, inhibited root activity, reduced the relative abundance of Geobacteria and Anaeromyxobacter , and consequently reduced the iron plaques on the root surfaces. Consequently, As uptake by the rice was inhibited. PSMP and PTFE reduced the hemoglobin content by directly destroying its tertiary structure, thereby retarding rice growth. In contrast, As increased the hemoglobin content by inducing reactive oxygen species in rice. Under the influence of PSMP, PTFE, and As, the activities of soluble starch synthase and pyrophosphorylase in rice grains were inhibited, and starch accumulation decreased. Thus, PSMP, PTFE, and As reduced rice biomass and yield owing to their physiological toxicity and adverse impacts on root activity. Grain yields in soil with an As content of 86.3 mg·kg –1 , 0.5% small particle-sized PSMP, and 0.5% small particle-sized PTFE decreased by 30.7%, 20.6%, and 19.4%, respectively, compared to the control. This study determined the comprehensive mechanism through which PSMP and PTFE affect As bioavailability, which is critical for managing rice biomass and low yields in As and microplastic co-contaminated soil.
研究领域:
环境科学
·
植物生理学
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土壤化学
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农学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Yingying Sun,Jie Chen,Wei Wang,Lizhong Zhu
Persistent organic pollutants (POPs) in agricultural soil often triggered metabolic alterations and phytotoxicity in plants, ultimately threatening crop quality. Unraveling the phytotoxic mechanisms of POPs in crops is critical for evaluating their environmental risks. Herein, the molecular mechanism of POP-induced phytotoxicity in rice ( Oryza sativa L.) was analyzed using metabolic profile, enzyme activity , and gene expression as linkages, including polycyclic aromatic hydrocarbons, polybrominated diphenyl ethers , polychlorinated biphenyls, and phthalate esters. Despite no observable changes in phenotypic traits (e.g., biomass and length of aboveground), the levels of reactive oxygen species (ROS) were promoted under stresses of the tested POPs, particularly 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), dibutyl phthalate (DBP), and di(2-ethylhexyl) phthalate (DEHP). Metabolomics analysis revealed that ROS contents positively correlated with metabolic perturbation levels ( r = 0.83), among which the galactose metabolism was significantly inhibited when exposed to DBP, DEHP, or BDE-47. The α-Galactosidase (α-Gal) involved in galactose metabolism was targeted as the key enzyme for the phytotoxicity of DBP, DEHP, and BDE-47, which was revealed by the inhibition of saccharide levels (45.5−82.1%), the catalytic activity of α-Gal (18.5−24.3%), and the gene expression (28.5−34.5%). Molecular docking simulation suggested that the three POPs occupied the active sites of α-Gal and formed a stable protein-ligand complex, thus inhibiting the catalytic activity of α-Gal. Partial least-squares regression analysis indicated that α-Gal activity was negatively associated with hydrogen bond acceptor, rotatable bond, and topological polar surface area of POPs. The results offered novel insights into the molecular mechanisms of phytotoxicity of POPs and provided important information for evaluating the environmental risk of POPs.
研究领域:
环境科学
·
植物生理学
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毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）