Journal of Zhejiang University-SCIENCE B
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2024
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IF 4.7
Jia Xiaodan,Lu Ke,Liang Xufang
Neuropeptide Y receptor Y8 (NPY8R) is a fish-specific receptor with two subtypes, NPY8AR and NPY8BR. Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation; this has been found in only a few fish, at present. In order to better understand the physiological function of npy8br , especially in digestion, we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology to generate npy8br −/− Japanese medaka ( Oryzias latipes ). We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability, ultimately affecting their growth. Specifically, npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes ( npy and agrp ). npy8br −/− medaka larvae fed for 10 d (10th day of feeding) still had incompletely digested brine shrimp ( Artemia nauplii ) in the digestive tract 8 h after feeding, the messenger RNA (mRNA) expression levels of digestion-related genes ( amy, lpl, ctra , and ctrb ) were significantly decreased, and the activity of amylase, trypsin, and lipase also significantly decreased. The deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes ( gh and igf1 ). Hematoxylin and eosin (H&E) sections of intestinal tissue showed that npy8br −/− medaka larvae had damaged intestine, thinned intestinal wall, and shortened intestinal villi. So far, this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.
研究领域:
分子生物学
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遗传学
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水产养殖
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生理学
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PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2024
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IF 3.9
Mengcheng Zhuo,Xi Wang,Yanhong Shi,Kun Chen,Xuchun Qiu
Organophosphorus pesticides (OPs), such as chlorpyrifos (CPF), are the most commonly used pesticides worldwide. Considering that OPs will eventually enter aquatic ecosystems due to runoff from agricultural lands, accidental leakage, and other unforeseen emergencies, monitoring water pollution of those substances is crucial for environmental protection and public health. In this study, Japanese medaka ( Oryzias latipes ) were exposed to CPF (0.03, 0.06, and 0.12 mg/L) for 6 h, and the time-series variations in their locomotor behavior and vocal traits were investigated. Compared with that measured before exposure, significantly changed locomotor behavior and vocal traits in Japanese medaka exposed to CPF could be observed at 4 h after exposure and thereafter, and the pattern of behavioral changes depends on the CPF concentrations. Exposure to CPF also changed the frequency-sound pressure level curve of Japanese medaka at 6 h after exposure, especially at 0.12 mg/L. Moreover, CPF exposure could significantly inhibit the acetylcholinesterase (AChE) activity in the brains and eyes of medaka, which exhibited significant correlations with the variation of locomotor behavioral and vocal traits. Considering that inhibiting the AChE activity is the primary mechanism underlying the neurobehavioral toxicity of all OPs, our finding suggested that simultaneously monitoring changes in the locomotor behavioral and vocal traits has a high potential to reflect the pollution of organophosphorus substances.
研究领域:
环境科学
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毒理学
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水生生物学
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生态毒理学
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PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
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2015
·Vol. 34
·期 11
·2611-2617
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IF 3.225
Lixia Li,Hongxing Chen,Ran Bi,Lingtian Xie
Chromium (Cr) is an essential element but is toxic to aquatic organisms at elevated concentrations. In the present study, adult Japanese medaka ( Oryzias latipes ) were exposed to a sublethal hexavalent chromium (Cr(VI)) concentration via dissolved and dietary exposures for 6 d. Various measurements of Cr were made: bioaccumulation in different tissues, subcellular distribution in the liver, effects on antioxidants and acetylcholinesterase (AChE), and Cr-induced lipid peroxidation. The results showed that bioaccumulation increased dramatically in all tested tissues from dissolved exposure but only significantly in the intestine from dietary treatment, implying that dissolved exposure may be predominant for Cr accumulation in medaka. Subcellular distribution revealed that Cr accumulated in the liver was mainly (46%) associated with the heat-stable protein fraction. Among the antioxidants examined, catalase (CAT) responded to dissolved Cr exposure in most tissues whereas superoxide dismutase (SOD) was less responsive. Malondialdehyde concentrations were significantly elevated in most tissues examined in the dissolved Cr-exposed fish, but were only elevated in the liver and intestine in the dietary Cr-exposed fish. The AChE activity in the brain was stimulated by 49% in the dissolved Cr-exposed fish. Reductions in condition factor and gonadosomatic index were also observed. These data help in an understanding of Cr tissue distribution and the acute effects of Cr in Japanese medaka. Environ Toxicol Chem 2015;34:2611–2617. © 2015 SETAC
研究领域:
环境毒理学
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鱼类生物学
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PubMed
高影响因子
AQUATIC TOXICOLOGY
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2015
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IF 3.451
Dan Li,Qin Chen,Jinling Cao,Hongxing Chen,Lixia Li,Nina Cedergreen,Haibo Xie,Lingtian Xie
One of the ultimate goals of green chemistry is to produce greener and more environmentally friendly chemicals to replace the existing toxic chemicals. In this study, Japanese medaka were exposed to 1.5 mg/L of bisphenol A or lignin-derived bisphenol for 60 days, and the expressions of various biochemical markers, effects on reproduction, and histopathology were evaluated. The results showed that concentrations of liver vitellogenin of LD-BP exposed males were approximately 125% higher compared to the control males. Total number of eggs from the BPA and LD-BP exposed fish was approximately 47% ( p < 0.001) and 25% ( p < 0.05) less than the control fish, respectively. Total number of brood was lower from the BPA (46%, p < 0.05) and LD-BP (17%, p < 0.05) exposed fish than that of the control fish. Relative to the control fish, catalase and glutathione- S -transferase were significantly affected by the two chemicals in all tested tissues. BPA and LD-BP caused lipid peroxidation in all the tested tissues. Furthermore, acetylcholinesterase and α-glucosidase activity were significantly inhibited. Histopathological analysis showed that both the testis and ovary were mildly damaged by both chemicals. LD-BP affected medaka slightly more severe than BPA except on the reproduction, which was most likely due to different uptake, translocation, binding to targets and metabolism. Our results demonstrated that chronic exposure to both chemicals caused several adverse effects to medaka. Further research on the toxicity of LD-BP to other aquatic organisms is needed before substitution of traditional BPA with LD-BP can be recommended.
研究领域:
环境科学
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毒理学
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生态学
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2017
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IF 3.743
Hongxing Chen,Zhongbao Guo,Yu Zhou,Dan Li,Lei Mu,Paul L. Klerks,Yongju Luo,Lingtian Xie
We previously demonstrated that chronic exposure to hexavalent chromium (Cr(VI)) causes a variety of adverse effects in the Japanese medaka ( Oryzias latipes ). The present study investigated the transition of acute to chronic effects by assessing the influences of Cr(VI) exposure concentration and exposure duration on Cr accumulation and their effects on fish growth and antioxidant physiology. Juvenile fish were exposed to 0.05, 0.5, 4 or 8 mg/L Cr(VI) for 28 days. Endpoints were evaluated on days 3, 7, 14, 21 and 28. In addition, Cr depuration was examined for fish from two exposure groups (0.5 and 8 mg/L). Chromium accumulation was rapid initially, then continued at a slower rate till the end of the exposure period without showing signs of reaching a steady state. Depuration patterns differed between the two exposure groups, but both reached 50% in 14 days. The rapid initial accumulation was accompanied by increased lipid peroxidation and elevated activities of antioxidants (e.g., GST , SOD and CAT). Activities of these enzymes had mostly returned to baseline levels by day 7, but there was no evidence of further cellular damage from ROS. Effects on fish length and weight continued to be evident over the 28-day exposure period. Our study suggest that the initial effects of Cr(VI) exposure may not be a good predictor of more-chronic effects in fish as a consequence of an efficient acclimation response by the antioxidant system that limits ROS-mediated toxicity.
研究领域:
环境毒理学
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鱼类生物学
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PubMed
高影响因子
AQUATIC TOXICOLOGY
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2016
·
IF 3.557
Hongxing Chen,Lei Mu,Jinling Cao,Jingli Mu,Paul L. Klerks,Yongju Luo,Zhongbao Guo,Lingtian Xie
Chromium (Cr) is an essential metal and a nutritional supplement for both human and agricultural uses. It is also a pollutant from a variety of industrial uses. These uses can lead to elevated Cr levels in aquatic environments, where it can enter and affect aquatic organisms. Its accumulation and subsequent effects in fish have received relatively little attention, especially for chronic exposure. In the present study, Japanese medaka were chronically exposed to dissolved or dietary Cr(VI) for 3 months. Cr accumulation in liver, gills, intestine, and brain was evaluated. Effects on the antioxidant system, nervous system (acetylcholinesterase, AChE), digestive system (α-glucosidase, α-Glu), and tissue histology (liver and gills) were also assessed. Cr accumulation was observed in the intestine and liver of fish exposed to Cr-contaminated brine shrimp. However, chronic dissolved Cr exposure led to significant Cr accumulation in all organs tested. Analysis of the subcellular distribution of Cr in medaka livers revealed that 37% of the Cr was present in the heat stable protein fraction. The dissolved Cr exposure had pronounced effects on the antioxidant system in the liver, with an elevated ratio of reduced glutathione/oxidized glutathione (GSH/GSSG) and decreases in GSH and glutathione S -transferase (GST). The α-Glu activity in the intestine was significantly inhibited. In addition, Cr exposure caused histopathological alterations in the gills and liver. In general, the effects of dietary Cr were relatively minor, possible due to the much lower accumulation in the fish. Our results imply that Japanese medaka accumulate Cr mainly via uptake of dissolved Cr(VI).
研究领域:
生态毒理学
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环境科学
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水生毒理学
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鱼类生理学
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金属累积
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DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
·
2025
·
IF 2.8
Pi Duan,Li Xiang,Xu Zitian,Wang Peitong,Liang Zhiying,Li Bingying,Guo Yafen,Huang Yongsi,Song Xiaohong,Wang Junjie,Wang Xuegeng
Due to the known adverse effects of bisphenol A (BPA), bisphenol F (BPF) and bisphenol S (BPS) have been widely adopted as substitutes. However, they are now detected at significant levels globally, often at levels rivaling or exceeding those of BPA, raises concerns regarding their potential ecological and health risks. Our previous work indicated that BPS exhibits lower toxicity than BPF in Chinese medaka (Oryzias sinensis). To validate this, we compared their toxicity in Japanese medaka (Oryzias latipes) using both acute and developmental exposure tests. In the 96-hr acute test, embryos exposed to concentration gradient showed a BPF median lethal concentration of 128 mg/L, while BPS exposure resulted in high survival even at the highest concentrations. Based on the observed acute toxicity, we investigated the underlying mechanism of BPF and evaluated vitamin C rescue. Vitamin C treatment effectively improved embryos survival rates. Subsequently, in the 15-day developmental exposure, 2 μg/L BPF significantly increased yolk sac edema and malformation rates. Bisphenol exposure did not show concentration-dependent malformation increases. Transcriptomic analysis revealed BPF induced more differentially expressed genes (DEGs) than BPS at the tested concentrations (0.02, 0.2, 2 μg/L): 177, 14 and 24 DEGs were identified in BPF-treated groups, respectively. In contrast, only 4, 2, and 12 DEGs were detected in the corresponding BPS-treated groups. Further analysis of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways enrichments provided further insights into the potential toxicological mechanisms of BPF. This study provides a comparative toxicity assessment of BPF and BPS, elucidates BPF's acute and developmental toxicity pathways, and offers toxicological data for risk assessment and regulation, which also provides a direction for in-depth research on toxicity mechanisms.
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PubMed
高影响因子
AQUATIC TOXICOLOGY
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2015
·
IF 3.451
Hongxing Chen,Jinling Cao,Lixia Li,Xing Wu,Ran Bi,Paul L. Klerks,Lingtian Xie
Maternal transfer of metals can be an important exposure route for animals. The maternal transfer of Cr and its effects on reproduction in fish are still largely unknown. In this study, Japanese medaka were exposed to a sublethal Cr(VI) concentration for 6 days (acute) and for 3 months (chronic). Chromium accumulation in the gonads, maternal transfer of Cr, and effects of Cr on the reproduction, histopathology and expressions of antioxidants in the gonads were evaluated. Both acute and chronic exposures resulted in significant Cr accumulation in gonads, eggs and larvae. In chronic Cr-exposed fish, approximately 61% of the Cr accumulated in the ovary was depurated by spawning during the first 3 days after exposure, suggesting that maternal transfer is a very important pathway for accumulation in offspring. The chronic exposure caused decreases in body weight, standard length, gonad weight, gonad-somatic index (GSI) and fecundity. The last of these was most severely affected: the total number of broods and eggs per female decreased by 57.1% and 75.9%, respectively. Moreover, egg weight and fertilization rate were also reduced (by approximately 20%) following chronic Cr(VI) exposure. Histopathological analyses showed that the Cr exposure resulted in the onset of follicular atresia and a reduction in the number of mature oocytes, along with a reduction in abundance of mature spermatozoa in testes. The GSH/GSSG ratio was greatly elevated after chronic Cr(VI) exposure, implying that GSH played a role in scavenging the reactive oxygen species generated by the reduction of Cr(VI) inside cells. This study provides evidence for the maternal transfer of Cr, highlights the importance of spawning in Cr depuration from the ovary, and demonstrates that chronic Cr(VI) exposure has serious impacts on reproduction in the Japanese medaka. Our results suggest that the issue of chronic Cr pollution deserves more attention than it has received to date.
研究领域:
毒理学
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水生生态学
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环境科学
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生殖生物学
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生态毒理学
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DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2026
·
IF 5.2
Xuchun Qiu,Ying Wu,Yue Liu,Mengcheng Zhuo,Kun Chen,Yuki Takai,IkJoon Kang,Yohei Shimasaki,Yuji Oshima
Pollution of chlorpyrifos (CPF), a widely utilized organophosphorus pesticide, presents potential risks to aquatic organisms. However, there is still a lack of information regarding the impacts of chronic CPF exposure on the vocal communication of sonic fish. In this study, we examined the effects of CPF (at 0, 0.3, and 3.0 μg/L for 21 days) on the locomotor behavior, vocal traits, and brain transcriptional profile of Japanese medaka ( Oryzias latipes ). The results indicated that exposure to CPF led to a significant reduction in exploratory behavior and social interaction, as well as alterations in the vocal traits of the Japanese medaka. In addition, CPF exposure significantly inhibited acetylcholinesterase activity in the eyes of Japanese medaka. Transcriptome analysis identified 68 and 1014 differentially expressed genes (DEGs) in medaka exposed to CPF at 0.3 μg/L (with 38 genes upregulated and 30 downregulated) and 3.0 μg/L (with 455 genes upregulated and 559 downregulated), respectively. The DEGs in both exposure groups were enriched in pathways associated with cardiac muscle contraction and adrenergic signaling in cardiomyocytes, suggesting that CPF may affect fish muscle function and thus modify their vocal traits. Furthermore, exposure to CPF at 3.0 μg/L also disrupted several critical biological pathways associated with the nervous and endocrine systems, providing insight into the underlying mechanisms responsible for the behavioral and vocal impairments induced by CPF. Given the crucial role that vocal signals play in survival and reproductive success, it is essential to examine further the ecological implications of CPF-induced alterations in the acoustic signals of sonic fish.
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高影响因子
PLoS One
·
2026
·Vol. 21
·期 5
·e0347661
·
IF 2.8
Lan-Chen Zhang,Yan Li,Ming-Fei Wu,Jin-Qiu Jia,Pan-Pan Jia
Zebrafish ( Danio rerio ), Marine medaka ( Oryzias melastigma ), and freshwater medaka ( Oryzias latipes ) are three major animal species in evaluation of environmental pollutant toxicity and building of human disease models with conserved physiological and molecular pathways. Bacterial strains from gut microbiota should firstly be isolated for their functions’ exploration. In this study, we optimized a culture-based workflow under aerobic and anaerobic conditions to recover intestinal bacteria from adult zebrafish (ZF), marine medaka (MM), and freshwater medaka (OL) using both dissection-based and in vivo sampling. The isolates were then identified by Gram staining, 16S rRNA gene sequencing, phylogenetic analysis, and API 20E-based biochemical characterization. The culturable bacteria isolated from gut microbiota were identified belonged to 14 genera in ZF, 12 genera in MM, and 18 genera in OL. Comparative analysis showed clear differences in the composition of cultured gut bacteria among the three fish species. Overall, the library and the differences of gut bacteria in three key fish models provided the potential applications of bacterial strains as the probiotics, to against the fish pathogens and to increase the pollutant toxicity-resistant. Moreover, the bacterial library will support the deep researches combined with germ-free (GF) animals to clarify the relationships of intestinal microbiota to host health.
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PubMed
高影响因子
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