INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Baolei Fan,Tingting Li,Xiaofang Song,Chao Wu,Chunqi Qian
A specific HPLC-MS/MS (High-Performance Liquid Chromatography with tandem Mass Spectrometry) method was developed and validated for simultaneous determination of several monosaccharides in three kinds Osmanthus fragrans Lour . After extraction, separation, protein removal, pigment removal and hydrolysis , monosaccharides was finally obtained from Osmanthus fragrans Lour . Positive ion mode detection and Multiple Reaction Monitoring (MRM) mode were used for quantitative analysis by PMP pre-column derivatization and Electrospray Ionization (ESI). Analysis and content determination of 6 monosaccharide components in 3 kinds of Osmanthus fragrans Lour . The HPLC separation was achieved on a Shim-pack VP-ODS6022748 (150 L × 2.0) with gradient elution at a flow rate of 0.2 ml/min in a run time of 40 min, and the mobile phase was acetonitrile-5 mmol/L ammonium acetate. PMP derivatization in HPLC-MS/MS can accurately measure Osmanthus fragrans Lour . mannose (Man), ribose (Rib), rhamnose (Rha), galacturonic acid (Gal UA), glucose (Glu), galactose (Gal), xylose (Xyl), fucose (Fuc). The results showed that HPLC-MS/MS pre-column derivatization method was simple and rapid, with small measurement error, but high sensitivity and good repeatability. The analysis of monosaccharide components in polysaccharide components has important practical significance.
研究领域:
植物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Xiaojing Sheng,Yingnan Lin,Jianmin Cao,Yang Ning,Xueli Pang,Jihong Wu,Fanyu Kong
Sweet osmanthus (Osmanthus fragrans Lour.) (OF) is one of the ten most famous flowers in China for its unique and delicate fragrance. A combined solid-phase microextraction and solvent-assisted flavor evaporation method was used to accurately capture the overall aromatic profile and characterize the predominant odorants of fresh osmanthus with the help of gas chromatography (GC)–olfactometry and comprehensive two-dimensional GC–quadrupole time-of-flight mass spectrometry (GC × GC–QTOF–MS). Twenty-six volatiles were identified for the first time in OF. A total of 23 potent odorants, dominated by monoterpene oxides and C6 aliphatic aldehydes, were identified. The efficacy of pectinase, β-glucosidase, and their combination on the aroma enhancement of OF was evaluated by quantitation of the target aroma components using GC–triple quadrupole–MS. The total concentration of key aroma components increased in all three enzyme treatment groups, and the increase was more significant in two β-glucosidase-treated groups. Changes in odor activity values and odor spectrum values of key odorants indicated that the pectinase-treated sample had more prominent floral, green, and potato-like scents. In contrast, the β-glucosidase-treated sample had more dominant floral, woody, almond-like, and fruity notes but less green odor, which was confirmed by sensory evaluation. β-Glucosidase and pectinase complement one another very well, and together, promote a remarkable aroma enhancement in OF.
研究领域:
农学
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食品科学
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分析化学
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植物生化
✓ 已验证
DOI
PubMed
高影响因子
Scientific Reports
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2017
·Vol. 7
·期 1
·1-10
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IF 4.259
Zhou Fei,Zhao Yajing,Li Maiquan,Xu Tao,Zhang Liuquan,Lu Baiyi,Wu Xiaodan,Ge Zhiwei
This study was aimed at investigating the chemical stability (the thermal, light and pH stability) of phenylethanoid glycosides (PhGs) in Osmanthus fragrans Lour. flowers, identifying the degradation products of acteoside and salidroside (major PhGs in O. fragrans flowers) by UPLC–QTOF–MS and studying the anti-hypoxia activity of PhGs after degradation. The degradation of PhGs followed first-order reaction kinetics, and the rate constant of acteoside (4.3 to 203.4 × 10 −3 day −1 ) was higher than that of salidroside (3.9 to 33.3 × 10 −3 day −1 ) in O. fragrans flowers. Salidroside was mainly hydrolyzed to tyrosol during storage, and the degradation products of acteoside were verbasoside, caffeic acid, isoacteoside, etc. In a model of cobalt chloride (CoCl 2 )-induced hypoxia in PC12 cells, the anti-hypoxia ability of PhGs decreased after degradation, which resulted from the reduction of PhGs contents. Particularly, caffeic acid exhibited stronger anti-hypoxia ability than acteoside and could slightly increase the anti-hypoxia ability of degraded acteoside. The results revealed that high temperature, high pH and light exposure caused PhGs degradation, and thus the anti-hypoxia ability of PhGs reduced.
研究领域:
药理学
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植物化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Applied Research on Medicinal and Aromatic Plants
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2020
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IF 1.857
Chenghui Pan,Laijun Zhao,Dayun Zhao
Osmanthus fragrans (Lour) flower is a typical fragrant herb in eastern countries containing multiple bioactive compounds, the extract of which has been widely used as a natural antioxidant additive in food and cosmetics. However, the existing extraction methods use organic solvents during the extraction process, which is non-eco-friendly and harmful to human health. In this study, we introduce, to our knowledge for the first time, microwave-assisted deep eutectic solvent extraction (MA-DESE) to extract antioxidant components from O. fragrans flower. The MA-DESE procedure was optimised, validated, and compared with that of traditional solvent extraction by using response surface methodology. The maximum antioxidant activity of 101.81 mg Trolox equivalent/g of dry weight was achieved at the following optimum MA-DESE parameters: microwave power, 497.12 W; microwave treatment time, 59.03 s; and solid–liquid ratio, 31.14 mL/g. In addition, UPLC-MS analysis showed a greater variety of antioxidant components, such as olivil and osmanthuside H, in O. fragrans flower extract obtained by MA-DESE than by the traditional ethanol reflux method. These results demonstrated that MA-DESE, which is safe and eco-friendly, could substitute for the conventional solvent extraction process, which is time-consuming and tedious, for the extraction of antioxidant components from O. fragrans flower.
研究领域:
植物学
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食品科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2015
·
IF 2.912
Yirong Jiang,Shuqin Mao,Weisu Huang,Baiyi Lu,Zengxuan Cai,Fei Zhou,Maiquan Li,Tiantian Lou,Yajing Zhao
Variations of phenylethanoid glycoside profiles and antioxidant activities in Osmanthus fragrans flowers through the digestive tract were evaluated by a simulated digestion model and UPLC/PDA/MS. Major phenylethanoid glycosides and phenolic acids, namely, salidroside, acteoside, isoacteoside, chlorogenic acid, and caffeic acid, were identified in four cultivars of O. fragrans flowers, and the concentration of acteoside was the highest, being up to 71.79 mg/g dry weight. After simulated digestion, total phenylethanoid glycoside contents and antioxidant activities were significantly decreased. Acteoside was identified as decomposing into caffeic acid, whereas salidroside was found to be stable during simulated digestion. According to Pearson’s correlation analysis, acteoside contents showed good correlations with antioxidant activities during simulated digestion (R2 = 0.994, P < 0.01). In conclusion, acteoside was the major contributor to the antioxidant activity of O. fragrans flowers, and salidroside was considered as the major antioxidant compound of O. fragrans flowers in vivo.
研究领域:
植物学
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药理学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2020
·
IF 4.049
Yongrui He,Meiling Qi
This work presents a new triptycene-based stationary phase (TP-PEG) combining the three-dimensional (3D) triptycene (TP) framework with polyethylene glycol (PEG) moieties for gas chromatographic (GC) separations. Its statically coated capillary column showed high column efficiency of 5263 plates/m determined by naphthalene at 120 °C. Its Rohrschneider-McReynolds constants and Abraham solvation system constants were measured to characterize its polarity and molecular interactions with analytes of different types. As evidenced, the TP-PEG column showed high-resolution performance for the isomers of anilines, phenols, halobenzenes and alkanes with distinct advantages over the PEG columns, particularly those critical isomers such as 3,5-/2,3-xylidine ( R = 2.94), m -/ p -chlorotoluene ( R = 1.92), p -/ m -cresol ( R = 1.89), 2,2-dimethylbutane/2-methylpentane ( R = 1.51), 2,2,3-trimethylbutane /2,3-dimethyl pentane ( R = 1.74) and 2,3-dimethylpentane/ n -heptane ( R = 1.92). In addition, it exhibited good column repeatability and reproducibility with the relative standard deviation (RSD) values of 0.02%–0.09% for run-to-run, 0.13%–0.22% for day-to-day and 2.7%–4.1% for column-to-column, respectively, and a wide operational temperature range (30 °C–280 °C) . Its application to GC–MS analysis of the essential oil of Osmanthus fragrans has proven its good potential for practical analysis of complex samples.
研究领域:
分析化学
·
色谱技术
✓ 已验证
DOI
PubMed
高影响因子
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