Colorimetric sensor for cysteine in human urine based on novel gold nanoparticles
Herein, a novel, high sensitive, and specific colorimetric sensor for cysteine (Cys) based on pectinase protected gold nanoparticles (P@AuNPs) has been demonstrated for the first time. The P@AuNPs were synthesized by “MW-assisted heat method” and were characterized by UV–vis, TEM , FT-IR and zeta potential techniques. Cys could cause the aggregation of P@AuNPs due to formation of the strong covalent Au-S bond and electrostatic binding. As the Cys concentration increased, the color of the solutions gradually changed from wine-red to blue as well as the large absorption band shifted from 523 to 650 nm upon P@AuNPs aggregation. In addition, various experimental parameters such as pH, the amount of P@AuNPs and incubation time were investigated for the optimum sensing conditions. The concentration of Cys could be determined by monitoring with the naked eye or a UV–vis spectrometer. The proposed colorimetric sensor showed an extreme selectivity toward the determination of Cys in the presence of 20-fold all other different interferents. Under optimum conditions, this method exhibited two good linear ranges from 4.85×10 −9 to 3.02×10 −4 M (R 2 =0.996) and 3.25×10 −3 to 1.03 × 10 −2 M (R 2 =0.999), with a low detection limit of 4.6×10 −9 M. Moreover, this colorimetric sensor was successfully applied to the detection of Cys in human urine samples, demonstrating its great value for practical application in biological systems.
危险品化学品经营许可证（带存储）