International Journal of Oral Science
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2026
·Vol. 18
·期 1
·50
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IF 17.6
Liu Xiya,Yuan Zuoying,Wan Zhuo,Yuan Xiaojing,Wang Yue,Gao Yike,Zhang Linxue,Song Rui,Sang Jingyi,Wang Yang,Han Yiming,Zhao Yuming,Wang Cun-yu
Porphyromonas gingivalis ( P. gingivalis ) invades host cells to evade antibiotics, driving the recurrence of periodontitis. However, current clinical treatment strategies remain ineffective in addressing this intracellular invasion by the bacteria. Here, we report a host-directed strategy that blocks bacterial invasion by targeting key host factors, including collagen I (using FT011), lipid rafts (using simvastatin), integrin β1 (using ATN-161), and FAK signaling (using YH-306). Inhibiting these targets significantly reduced P. gingivalis infection in human oral keratinocytes and periodontal ligament stem cells. Crucially, simvastatin restored the osteogenic ability of P. gingivalis -infected PDLSCs. These drugs subsequently functioned as effective antibiotic adjuvants. The combination of simvastatin or YH-306 with metronidazole, a standard-of-care antibiotic for periodontitis, exhibited potent synergistic effects, significantly eradicating intracellular bacteria at reduced antibiotic dosages. In a rat periodontitis model, simvastatin-metronidazole therapy yielded superior outcomes, significantly mitigating bacterial load, inflammation, and alveolar bone loss. The repurposing of clinically available drugs, such as simvastatin, as antibiotic adjuvants represents a promising, readily translatable therapeutic approach for periodontitis.
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Microbiology Spectrum
·
2025
·
IF 3.8
Ru Qu,Ming Li,Ping Li,Ying Song,Biao Dong,Juan Liu,Xuan Mo,Zhenjiang Zech Xu,Xiaochang Huang
Porphyromonas gingivalis is a well-known periodontal pathogen that has also been linked to systemic diseases. In this study, our results revealed that P. gingivalis exacerbated inflammation, as indicated by weight loss, decreased colon length, and increased colonic tissue inflammation. Furthermore, P. gingivalis increased the population of Th17 cells in the spleen and upregulated the expression of pro-inflammatory cytokines in mice. Additionally, P. gingivalis aggravated the gut microbiota dysbiosis by reducing the beneficial bacteria like S24-7. In addition, we discovered that paeoniflorin, a widely recognized biologically active phytochemical, had the potential to mitigate the exacerbated inflammation caused by P. gingivalis. Specifically, paeoniflorin treatment rebalanced immune cell populations, reduced the levels of inflammatory cytokines, and reshaped the composition of the gut microbiota. In summary, our findings suggest that paeoniflorin holds promise as a potential therapeutic agent for alleviating the inflammatory effects induced by P. gingivalis.
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International Journal of Nanomedicine
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2020
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IF 5.115
Gaofeng Liang,Hao Shi,Yijun Qi,Jinghua Li,Aihua Jing,Qiwei Liu,Wenpo Feng,Guangda Li,Shegan Gao
Introduction Biofilms protect bacteria from antibiotics and this can produce drug-resistant strains, especially the main pathogen of periodontitis, Porphyromonas gingivalis. Carbon quantum dots with various biomedical properties are considered to have great application potential in antibacterial and anti-biofilm treatment.Methods Tinidazole carbon quantum dots (TCDs) and metronidazole carbon quantum dots (MCDs) were prepared by a hydrothermal method with the clinical antibacterial drugs tinidazole and metronidazole, respectively. Then, TCDs and MCDs were characterized by transmission electron microscopy, UV–visible spectroscopy, infrared spectroscopy and energy-dispersive spectrometry. The antibacterial effects were also investigated under different conditions.Results The TCDs and MCDs had uniform sizes. The results of UV–visible and energy-dispersive spectrometry confirmed their important carbon polymerization structures and the activity of the nitro group, which had an evident inhibitory effect on P. gingivalis, but almost no effect on other bacteria, including Escherichia coli, Staphylococcus aureus and Prevotella nigrescens. Importantly, the TCDs could penetrate the biofilms to further effectively inhibit the growth of P. gingivalis under the biofilms. Furthermore, it was found that the antibacterial effect of TCDs lies in its ability to impair toxicity by inhibiting the major virulence factors and related genes involved in the biofilm formation of P. gingivalis, thus affecting the self-assembly of biofilm-related proteins.Conclusion The findings demonstrate a promising new method for improving the efficiency of periodontitis treatment by penetrating the P. gingivalis biofilm with preparations of nano-level antibacterial drugs.
研究领域:
口腔医学
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纳米生物学
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Biomaterials Science
·
2023
·
IF 6.6
Qinghua Cao,Xiang Xiao,Chengcheng Tao,Rui Shi,Rui Lv,Ruochen Guo,Xinyi Li,Baiyan Sui,Xin Liu,Jian Liu
As a key pathogen of periodontitis, P. gingivalis requires support of the initial colonizing bacterium (S. gordonii preferably) to form symbiotic biofilms on gingival tissues with enhanced antibiotic resistance. Here, we report a new strategy to treat periodontitis biofilms with S. gordonii membrane-coated H2O2 self-supplied nanocomposites (ZnO2/Fe3O4@MV NPs) in a “Jenga” style. Integration of our special MV coatings enables selectively enhanced internalization of the cargos in S. gordonii, thus inducing severe damage to the foundational bacterial layer and collapse/clearance of symbiotic biofilms consequently. This strategy allows us to clear the symbiotic biofilms of S. gordonii and P. gingivalis with active hydroxyl radicals (˙OH) derived from ZnO2–Fe3O4@MV NPs in a H2O2 self-supplied, nanocatalyst-assisted manner. This “Jenga-style” treatment provides a cutting-edge proof of concept for the removal of otherwise robust symbiotic biofilms of periodontitis where the critical pathogens are difficult to target and have antibiotic resistance.
研究领域:
牙科学
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纳米技术
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微生物学
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材料科学
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CARBOHYDRATE POLYMERS
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2021
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IF 9.381
Tao Wu,Lin Huang,Jing Sun,Jiahui Sun,Qi Yan,Bo Duan,Lina Zhang,Bin Shi
The guided tissue regeneration technique is an effective approach to repair periodontal defect. However, collagen barrier membranes used clinically lose stability easily, leading to soft tissue invasion, surgical site infection, and failure of osteogenesis. An ideal barrier membrane should possess proper antibacterial, osteoconductive activities, and favorable biodegradation. In this study, zinc oxide nanoparticles were homogeneously incorporated into the chitin hydrogel (ChT-1%ZnO) through one-step dissolution and regeneration method from alkaline/urea solution the first time. The remaining weights of ChT-1%ZnO in 150 μg/mL lysozyme solution was 52% after 5 weeks soaking. ChT-1%ZnO showed statistical antibacterial activities for P. gingivalis and S. aureus at 6 h, 12 h, and 24 h. Moreover, ChT-1%ZnO exhibits osteogenesis promotion in vitro , and it was further evaluated with rat periodontal defect model in vivo . The cemento-enamel junction value in ChT-1%ZnO group is 1.608 mm, presenting a statistical difference compared with no-membrane (1.825 mm) and ChT group (1.685 mm) after 8 weeks postoperatively.
研究领域:
牙科
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生物材料
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纳米技术
·
组织工程
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