APPLIED CATALYSIS B-ENVIRONMENTAL
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2023
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IF 22.1
Qingjie Luan,Xiangdong Xue,Hongtai Feng,Lu Tao,Dongxue Zhou,Tengfei Chen,Mengxi Tan,Wenjun Dong
Molecular-type cocatalysts can achieve the maximum electron transfer and minimum light absorption loss by virtue of their size advantage. Herein, a novel hydrophilic molecule-semiconductor hybrid P 4 @Vs-ZnIn 2 S 4−x photocatalyst was successfully developed by in-situ P 4 molecular anchoring on S defect ZnIn 2 S 4−x (V S -ZnIn 2 S 4−x ). The electron-rich P 4 molecules are anchored on the Zn-S 4 surface through S vacancies, forming Zn-P and S-P bonds that can promote electron injection from the electron-rich P 4 molecular to Vs-ZnIn 2 S 4−x substrate and reduce the adsorption/desorption barrier of H intermediate on S atom of Zn-S 4 plane. Meanwhile, the electrostatic potential depression around P 4 promoted the directional migration of in-plane photogenerated electrons, and pooled electrons to the highly active P sites and S sites, thereby accelerating the kinetics of hydrogen evolution. As a result, P 4 @Vs-ZnIn 2 S 4−x achieves an optimal photocatalytic hydrogen evolution activity of 26.1 mmol g −1 h −1 under visible light, which is 6.2 times higher than 4.2 mmol g −1 h −1 for Vs-ZnIn 2 S 4−x .
研究领域:
材料科学
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化学
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DOI
高影响因子
MOLECULAR MEDICINE
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2024
·Vol. 30
·期 1
·1-16
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IF 6
Xie Bin,Chen Qiong,Dai Ziyu,Jiang Chen,Chen Xi
Background Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory lung disease associated with high morbidity and mortality worldwide. Oxidative injury and mitochondrial dysfunction in the airway epithelium are major events in COPD progression.Methods and resultsThe therapeutic effects of Progesterone (P4) were investigated in vivo and in vitro in this study. In vivo, in a cigarette smoke (CS) exposure-induced COPD mouse model, P4 treatment significantly ameliorated CS exposure-induced physiological and pathological characteristics, including inflammatory cell infiltration and oxidative injury, in a dose-dependent manner. The c-MYC/SIRT1/PGC-1α pathway is involved in the protective function of P4 against CS-induced COPD. In vitro, P4 co-treatment significantly ameliorated H2O2-induced oxidative injury and mitochondrial dysfunctions by promoting cell proliferation, increasing mitochondrial membrane potential, decreasing ROS levels and apoptosis, and increasing ATP content. Moreover, P4 co-treatment partially attenuated H2O2-caused inhibition in Nrf1, Tfam, Mfn1, PGR-B, c-MYC, SIRT1, and PGC-1α levels. In BEAS-2B and ASM cells, the c-MYC/SIRT1 axis regulated P4’s protective effects against H2O2-induced oxidative injury and mitochondrial dysfunctions.Conclusion P4 activates the c-MYC/SIRT1 axis, ameliorating CS-induced COPD and protecting both airway epithelial cells and smooth muscle cells against H2O2-induced oxidative damage. PGC-1α and downstream mitochondrial signaling pathways might be involved.
研究领域:
呼吸病学
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药理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
POLYMER
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2025
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IF 4.5
Naifan Dong,Jintang Duan,Cailiang Zhang,Xueping Gu,Lianfang Feng,Liu Liu
Polysiloxanes with amino functional groups exhibit remarkable backbone flexibility, lubricity, and softness, positioning them a promising class of polymer materials. The phosphazene base (Bu-t-P 4 ) serves as an effective catalyst for the ring-opening copolymerization (ROCP) of octamethylcyclotetrasiloxane (D 4 ) and N-β-(aminoethyl)-γ-aminopropylmethyldiethoxysilane (KH-602). Under catalysis by 100 ppm of Bu-t-P 4 at 110 °C for 1 h, high yield of 93.59 % was achieved, resulting in amino silicone oil with a number-average molecular weight of 4.3 × 10 3 g/mol, and a narrow molecular weight distribution (2.00). Fourier-transform infrared spectroscopy and nuclear magnetic resonance (NMR) confirmed that the product's structure corresponds to expected chemical shifts. In situ Raman spectroscopy monitoring and NMR analysis was employed to propose and validate the polymerization mechanism within the Bu-t-P 4 -catalyzed system involving both cyclic and linear monomers. This study demonstrates an effective strategy, affirming that Bu-t-P 4 can be used as an efficient organic catalyst for the modified polysiloxanes.
研究领域:
材料科学
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有机化学
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产品关联
DOI
高影响因子
Frontiers in Plant Science
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2022
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IF 6.627
Wang Juanying,Zhao Hanyu,Chen Ting,Lin Wenxiong,Lin Sheng
Background: Plant growth-promoting bacteria (PGPB) represents a common biological fertilizer with remarkable effect in improving crop production and environmental friendliness.Methods: In the present work, we presented a detailed characterization of plant morphology and physiology, metabolism, and pharmacological activity of A. roxburghii between Burkholderia ambifaria LK-P4 inoculation and un-inoculation (CK) treatment by routine analytical techniques (include microscopy and enzymatic activity assays and so on) coupled with metabolomics approaches.Results: Morphological and physiological results showedthat the P4 bacteria could significantly increase plant stomatal density, freshweight, survival rate,and the content of total flavonoids in leaves but reducethe amount of free amino acid. Furthermore, metabolite data showed that fatty acids (linoleic acid, linolenic acid, stearic acid) and active substance (kyotorphin and piceatannol) were specifically up-regulated in P4 inoculation. It was also demonstrated that the differential metabolites were involved in citrate cycle, glyoxylate and dicarboxylate metabolism, and biosynthesis of unsaturated fatty acids pathway. In addition, pharmacological efficacy found that A. roxburghii under P4 inoculation can significantly decrease (p < 0.05) blood glucose levels and protect the organs of mice with similar effect of Glibenclamide tablets.Conlusion: Overall, it can be seen that the exogenous P4 bacteria can promote the growth and increase content of special metabolites in A. roxburghii. This study provided theoretical basis and supported for the high-yield and high-quality bionic cultivation of A. roxburghii.
研究领域:
植物学
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微生物学
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药理学
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DOI
PubMed
高影响因子
Journal of Cleaner Production
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2023
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IF 11.1
Guanting Liu,Yuanjia Ma,Xiao Yang
White phosphorus (P 4 ) is an essential raw material for the production of phosphorus chemicals. The conventional carbothermal reduction process used to extract P 4 from phosphate rock is known as energy intensive and environmentally polluting, posing obstacles to meeting the urgent global demands for clean production and carbon neutrality . Moreover, the low product purity restricts its application in high-value industries. This paper presents an original approach that is both simple and coke-free for extracting high-purity P 4 . The method involves electrolyzing dissolved phosphate rock in molten CaCl 2 at a mild temperature. We undertake an in-depth investigation into the dissolution behavior of phosphate rock in molten CaCl 2 and the electrolysis behavior of the melt. The experiments demonstrate the rapid dissolution of phosphate in the rock when exposed to molten CaCl 2 , reaching saturation within a short time (e.g., within 10 min at 850 °C). The dissolution of phosphate shows a preference over silicates present in the phosphate rock. The electrochemical reduction of PO 4 3− in molten CaCl 2 is electrochemically reversible, as observed at potential scan rates not exceeding 0.20 V s −1 . The obtained P 4 product through electrolysis is of higher purity compared to the industrial-grade commercial product produced via carbothermal reduction. The implementation of pre-electrolysis can further improve the purity of the product and the current efficiency of the electrolysis process. The theoretical electricity and carbon consumption in the electrolysis method are estimated to be 9.12 kWh and 0.53 kg, respectively, for every 1 kg of P 4 produced. These values are lower than the conventional carbothermal reduction process. This cutting-edge electrolysis method represents a paradigm shift towards a more sustainable development of the phosphorus chemical industry, thereby showcasing its potential for revolutionizing the field.
研究领域:
化学工程
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环境科学
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材料科学
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产品关联
DOI
高影响因子
eLife
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2022
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IF 8.713
Gang Zhai,Tingting Shu,Guangqing Yu,Haipei Tang,Chuang Shi,Jingyi Jia,Qiyong Lou,Xiangyan Dai,Xia Jin,Jiangyan He,Wuhan Xiao,Xiaochun Liu,Zhan Yin
Disruption of androgen signaling is known to cause testicular malformation and defective spermatogenesis in zebrafish. However, knockout of cyp17a1, a key enzyme responsible for the androgen synthesis, in ar-/- male zebrafish paradoxically causes testicular hypertrophy and enhanced spermatogenesis. Because Cyp17a1 plays key roles in hydroxylation of pregnenolone and progesterone (P4), and converts 17α-hydroxypregnenolone to dehydroepiandrosterone and 17α-hydroxyprogesterone to androstenedione, we hypothesize that the unexpected phenotype in cyp17a1-/-;androgen receptor (ar)-/- zebrafish may be mediated through an augmentation of progestin/nuclear progestin receptor (nPgr) signaling. In support of this hypothesis, we show that knockout of cyp17a1 leads to accumulation of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and P4. Further, administration of progestin, a synthetic DHP mimetic, is sufficient to rescue testicular development and spermatogenesis in ar-/- zebrafish, whereas knockout of npgr abolishes the rescue effect of cyp17a1-/- in the cyp17a1-/-;ar-/- double mutant. Analyses of the transcriptomes among the mutants with defective testicular organization and spermatogenesis (ar-/-, ar-/-;npgr-/- and cyp17a-/-;ar-/-;npgr-/-), those with normal phenotype (control and cyp17a1-/-), and rescued phenotype (cyp17a1-/-;ar-/-) reveal a common link between a downregulated expression of insl3 and its related downstream genes in cyp17a-/-;ar-/-;npgr-/- zebrafish. Taken together, our data suggest that genetic or pharmacological augmentation of the progestin/nPgr pathway is sufficient to restore testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling.
研究领域:
生物学
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生殖生物学
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内分泌学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENT INTERNATIONAL
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2020
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IF 7.577
Miaoling Lin,Rui Hua,Jing Ma,Yao Zhou,Pei Li,Xiya Xu,Zhiqiang Yu,Song Quan
Background Bisphenol A (BPA) is a widespread endocrine-disrupting chemical with estrogen like effects, which could interfere with the human reproductive system by disrupting the normal function of granulosa cells (GCs) leading to abnormal ovarian function. However, the mechanism of its toxicity on human GCs has not been clearly described thus far. Methods 106 normogonadotropic infertile women undergoing their first in-vitro fertilization-embryo transfer (IVF-ET) cycle were recruited. Urinary BPA level and the early outcomes of IVF-ET were analysed. Patients were divided to low and high BPA exposure groups using the median urinary BPA concentration as the cut-off value. In-vivo and in-vitro studies were conducted using mice and human granulosa cell line (KGN cells). Female Kunming mice approximately 6–8 weeks of age were poisoned with BPA at different dosages (1, 10 or 100 μg/kg) by oral gavage once daily for 2 weeks, while KGN cells were exposed to BPA at the concentration of 1, 10 or 100 nM for 24 h, 48 h or 72 h. BPA-induced ovarian morphologic changes were analysed by histopathology investigation. Cell viability and apoptosis were evaluated using CCK-8, TUNEL and flowcytometric, respectively. Hormone levels were determined using ELISA and the molecular mechanism studies were conducted using immunofluorescence, RT-PCR and western blots. Results The oocyte retrieval rate, maturation rate and embryo implantation rate significantly decreased with the higher level of urinary BPA concentration. Peak E2 level was lower in high BPA group, but no statistical significance could be observed. In BPA treated mice, cystic dilation of the follicles with a decreased number of GCs could be observed histopathologically. Decreased E2, P4 and AMH level and GCs autophagy could be detected both in-vivo and in-vitro with the activation of AMPK/mTOR/ULK1 signalling pathway. As being confirmed in KGN cells, phosphorylated AMPK and ULK1 increased while phosphorylated mTOR decreased, and by inhibition autophagy using knockdown of AMPK or 3-MA, adverse effects of BPA exposure in-vitro could be reversed. Conclusion BPA exposure might abnormally influence human ovarian functions leading to abnormal folliculogenesis by activation of autophagy in GCs through AMPK/mTOR/ULK1 pathway.
研究领域:
内分泌学
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生殖生物学
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毒理学
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细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）