Processes
·
2025
·Vol. 13
·期 3
·731
·
IF 2.8
Shuanghu Fan,Zihan Feng,Meiting Xu,Zhenxia Shi,Yufeng Zhang,Peipei Zhang,Xiaoqiang Hou
Phthalic acid esters (PAEs) are mainly used as plasticizers and result in serious environmental contamination worldwide. Microbial biodegradation becomes an efficient strategy for PAE elimination. In the current study, the PAE-degrading strainGordoniasp. LUNF6 was isolated from contaminated soil. Strain LUNF6 can efficiently degrade DBP in a wide range of temperatures, pH values, and salinity levels. This strain is also capable of degrading 11 types of PAEs and displays remediation potential in wastewater. The complete genome of strain LUNF6 was sequenced to determine its efficient degradation performance. Its genome comprises a chromosome (3,971,257 bp) and a plasmid (78,813 bp). After gene function annotation, the complete PAE degradation pathway was proposed. The gene of monoalkyl PAE hydrolase MphGd2 was cloned and heterologously expressed. The protein of MphGd2 was purified by infinity chromatography, and we hydrolyzed MBP to produce PA. These results reveal the molecular mechanism of PAE degradation by strain LUNF6, which will contribute to the application of strain LUNF6 and hydrolase MphGd2 in bioremediation.
研究领域:
环境微生物学
·
分子生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2022
·
IF 4.907
Xiaoyong Song,Zihan Zhang,Yanran Dai,Deshou Cun,Baihui Cui,Yuewei Wang,Yaocheng Fan,Haibin Tang,Liang Qiu,Feihua Wang,Dongru Qiu,Wei Liang
Di-(2-ethylhexyl) phthalate (DEHP), the most extensively used phthalate acid esters (PAEs), poses a potential risk to human and environment. A novel bacterial strain , Rhodococcus sp. LW-XY12, with efficient PAE-degrading capability, was isolated from activated sludge. Strain LW-XY12 could degrade 96.91 ± 0.68% of DEHP (500 mg L −1 ) within 32 h. The degradation curves of DEHP (50–1500 mg L −1 ) and PAE mixture (500 mg L −1 each) fitted well with the modified Gomperz kinetics model. DEHP metabolic pathway was reconstructed by using genome annotation and metabolic intermediate analyses. The DEHP metabolic pathway might comprise de-esterification and β-oxidation. Homologous modeling and molecular docking analysis revealed that DEHP and MEHP (mono-(2-ethylhexyl) phthalate) could be bound to putative carboxylesterase (KXC42_04905) via hydrogen bonding and hydrophobic interaction. A conserved catalytic triad (Ser195-Glu319-His412) might act as an active protein pocket and catalyze the hydrolysis of DEHP. Carboxylesterase (KXC42_04905) could hydrolyze ester bond and a possible mechanism underlying ester bond hydrolysis catalyzed by the carboxylesterase was proposed. Protocatechuate 3,4-dioxygenase, 3-oxoadipate CoA-transferase, benzoate 1,2-dioxygenase, catechol 1,2-dioxygenase, and catechol 2,3-dioxygenase transcripts were detected and significantly up-regulated under DEHP induction. This study sheds light on the functional genes associated with DEHP degradation and metabolic mechanism in this versatile bacterium for degradation of PAEs.
研究领域:
环境微生物学
·
基因组学
·
生物降解
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Hai-Ming Zhao,Rui-Wen Hu,Huan Du,Xiao-Ping Xin,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Jie-Sheng Liu,Dong-Mei Zhou,Ming-Hung Wong,Zhen-Li He
Microbial degradation is considered the most promising method for removing phthalate acid esters (PAEs) from polluted environments; however, a comprehensive genomic understanding of the entire PAE catabolic process is still lacking. In this study, the repertoire of PAE catabolism genes in the metabolically versatile bacterium Rhodococcus sp. 2G was examined using genomic, metabolic, and bioinformatic analyses. A total of 4930 coding genes were identified from the 5.6 Mb genome of the 2G strain, including 337 esterase/hydrolase genes and 48 transferase and decarboxylase genes that were involved in hydrolysing PAEs into phthalate acid (PA) and decarboxylating PA into benzoic acid (BA). One gene cluster ( xyl ) responsible for transforming BA into catechol and two catechol-catabolism gene clusters controlling the ortho ( cat ) and meta ( xyl & mhp ) cleavage pathways were also identified. The proposed PAE catabolism pathway and some key degradation genes were validated by intermediate-utilising tests and real-time quantitative polymerase chain reaction. Our results provide novel insight into the mechanisms of PAE biodegradation at the molecular level and useful information on gene resources for future studies.
研究领域:
微生物学
·
环境科学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2015
·
IF 4.143
Hai-Ming Zhao,Huan Du,Lei Xiang,Yi-Liang Chen,Lei-An Lu,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo
Phthalate ester (PAE) accumulation in crops poses great risks to human health and has aroused great concern. Here, we investigated variations in di- n -butylphthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) accumulation by various Chinese flowering cabbage cultivars and revealed their variation mechanism. There were significant differences ( p < 0.05) in shoot PAE concentrations of 28 cultivars. Moreover, significant positive correlations between DBP and DEHP concentrations in shoots of all cultivars indicated that they could be taken up simultaneously by various cultivars. Due to the lower translocation factor of low-PAE accumulator, its shoot PAEs concentrations were much lower than root compared to high-PAE accumulator. Further, subcellular distribution showed that PAE concentrations of root cell walls and organelles were much higher than those of shoots in low-PAE accumulator. Therefore, lower translocation from root to shoot and more PAEs accumulating in cell walls and organelles of root might act as main formation mechanism of low-PAE accumulator.
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Yazhou Chen,Li Zhang,Yingjie Liu,Shiming Tan,Ruidan Qu,Zirong Wu,Yue Zhou,Jing Huang
Poly-γ-l-glutamic acid (PGA) is an outstanding drug carrier candidate owning to its excellent biodegradability and biocompatibility. The PGA carrier may shield toxic drugs from the body and enable the delivery of poorly soluble or unstable drugs and thereby minimize the side effects and improve drug efficacy. However, the limitation of PGA as a drug carrier is low drug loading efficiency (DLE), which is usually below 30%. In this study, we reported a chemical modification method using l-phenylalanine ethyl ester (PAE). PGA–PAE construct was amphiphilic, which could form micelles in aqueous solution. Cisplatin (CDDP), a commonly used chemotherapy drug whose side effect is well-known, was used as a model molecule to test the drug-loading efficiency of PGA–PAE. In this paper, two sizes of CDDP-loaded PGA–PAE micelles (M(Pt)-1 and M(Pt)-2) were prepared, the average diameter of M(Pt)-1 was 106 ± 6 nm and M(Pt)-2 was 210 ± 9 nm. The DLE of M(Pt)-1 and M(Pt)-2 was 52.8 ± 2.2 and 55.8 ± 1.2%, respectively. Both exhibited excellent biocompatibility, stability, and drug-retaining capability in physiological condition. The in vitro accumulative drug-releasing profile, IC50 for different tumor cell lines HeLa, A549, and HCCC9810, and in vivo pharmacokinetics were similar between these two micelles; however, M(Pt)-1 showed higher tumor tissue retention and longer efficient cancer cell internalization time (up to 20 d). Our results suggested PGA–PAE micelle carriers reduced the toxicity of CDDP and its size at around 100 nm was the better for CDDP high-efficacy.
研究领域:
药理学
·
肿瘤学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
Processes
·
2025
·Vol. 13
·期 10
·3245
·
IF 2.8
Peng Peng,Shuanghu Fan,Meiting Xu,Liyuan Liu,Xiaolin Zhang,Zihan Feng,Haina Du,Zimeng Wang,Qiao Qin,Weiming Feng,Hongyan Liu,Jingjing Guo
Phthalic acid esters (PAEs) are important plasticizers that have led to the heavy pollution of farmland, which has aroused significant and widespread concern for soil health and food safety. Microbial degradation has been recognized as an efficient pathway for removing PAEs from the environment. In this study, the PAE-degrading strain FR1 was isolated from sewage and determined to belong toGlutamicibacter. This strain degraded PAEs efficiently under a wide range of conditions—10–50 °C, pH of 6.0–11.0, and 0–8% salinity—demonstrating its great potential in PAE bioremediation. Genome sequencing provided complete genomic information, showing that the strain comprises one chromosome (3,404,214 bp) and three plasmids (112,089 bp, 80,486 bp, and 40,002 bp). The chromosome harbors 3238 protein genes, of which the PAE hydrolase genesdphGB1andmphGB2have been cloned. The hydrolase DphGB1 from hydrolase family I contained the catalytic triad Ser75-Asp194-His221. After heterogeneous expression and purification, the recombinant protein DphGB1, of about 30 kDa, was obtained. This hydrolase showed strong hydrolytic ability toward DEHP. The protein MphGB2 could also hydrolyze MBP. The molecular docking revealed interaction between DphGB1 and DBP. The main hydrolases of strain FR1-degrading PAEs were functionally identified. These results will promote the elucidation of the catalytic mechanisms of PAE hydrolases and the application of strain FR1 in farmland soil remediation.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Dan Ma,Yang Lyu,Yujia Wang,Xiaochen Li,Junheng Wang,Yingying Wang,Sijun Dong
Phthalates (PAEs), such as dibutyl phthalate (DBP), are emerging contaminants in agriculture that are commonly used in plastic films, pesticides, and soil amendments. Their accumulation in water threatens ecosystems and human health. Biodegradation offers a promising solution, but the DBP degradation mechanisms remain unclear. This study identified two 4,5-PCD isoenzymes (PCD34 and PCD67) in Curvibacter sp. PAE-UM. PCD34 showed higher affinity and broader substrate specificity, while PCD67 had better thermal stability and metal ion sensitivity. Both enzymes converted protocatechuate (PCA) to 4CHMS at 45% efficiency in 5 min. Molecular dynamics revealed the stable binding of PCD67, while mutagenesis enhanced PCD34 activity (124.4%) by reducing steric hindrance. These findings may aid in the engineering of strains for PAE bioremediation in agricultural wastewater.
研究领域:
环境微生物学
·
生物技术
·
酶学
·
分子生物学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Coatings
·
2026
·Vol. 16
·期 6
·711
·
IF 3.4
Hui Liu,Yu Zhu,Xin Cheng,Zhenhua Dong,Qiang Liu
Superhydrophobic materials offer promising prospects for utilization in energy, environmental, and related fields. However, their long-term stability in natural environments is constrained by factors such as mechanical wear and aging, which compromise their practical effectiveness and service life. While notable experimental results have been obtained worldwide, scalable application remains limited by the complexity of the requisite fabrication processes. In this study, a durable superhydrophobic coating was developed through a facile one-step process, utilizing a polyaspartic ester (PAE) matrix reinforced with a composite of self-synthesized fluorinated silica (F-SiO2) and hexagonal boron nitride (h-BN) micro-/nano-structures. This strategy effectively enhanced filler dispersion within the resin matrix and promoted hydrophobicity, yielding a stable superhydrophobic surface. The resulting coating exhibits significant potential for scalable application. The optimized coating demonstrated a water contact angle of 161.2° and a roll-off angle of 7.6°, showing excellent repellency to water, corrosive liquids, and fluids across a wide pH range, along with remarkable self-cleaning performance. Benefiting from the synergistic enhancement of h-BN and F-SiO2, the coating also exhibits superior mechanical durability, maintaining a contact angle of 144.4° after 1000 abrasion cycles. Furthermore, in low-temperature anti-icing tests, the coating significantly delayed ice formation on its surface. Notably, after 1000 h of UV aging tests, the F-SiO2@BN/PAE coating retained its intact superhydrophobic structure, with the water contact angle only slightly decreasing from 159.6° to 152.8°, still within an excellent superhydrophobic state, demonstrating outstanding weather resistance. By integrating surface functionalization with mechanical reliability through a facile one-step fabrication process, this study provides significant insights for the large-scale application of hydrophobic materials in the energy and transportation sectors.
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2016
·Vol. 40
·期 11
·9806-9812
·
IF 3.277
Zhibing Sun,Dezhong Zhou
Three oligomeric linear poly(beta-amino esters) (PAEs) were synthesized and used to improve the gene transfection efficiency and reduce the cytotoxicity of poly(L-lysine) (PLL). The synthesized PAEs can co-assemble with PLL and plasmid DNA to form PLL/PAE/DNA ternary complexes of nanoscale sizes and moderate surface charges. The existence of multiple tertiary amines on PAE backbones imparts the ternary complexes with strong proton buffering capacity and therefore enhances the endosomal escape ability of the ternary complexes. In HeLa cells, the PLL/PAE/DNA ternary complexes show up to 476-fold enhancement in gene transfection efficiency when compared with the PLL/DNA binary complexes. Meanwhile, the incorporation of PAE oligomers significantly reduced the cytotoxicity of PLL. The strategy proposed here may provide insights into the development of PLL based gene delivery systems.
研究领域:
生物化学
·
分子生物学
·
基因治疗
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2020
·
IF 5.715
Pei-Pei Du,Yu-Hong Huang,Huixiong Lü,Lei Xiang,Yan-Wen Li,Hui Li,Ce-Hui Mo,Quan-Ying Cai,Qing X. Li
Phthalic acid esters (PAEs) is a class of prevalent pollutants in agricultural soil , threating food safety through crop uptake and accumulation of PAEs. Accumulation of PAEs varies largely among crop species and cultivars. Nevertheless, how root exudates affect PAE bioavailability , dissipation, uptake and accumulation is still not well understood. In the present study, desorption and pot experiments were designed to investigate how root exudates from high-( Peizataifeng ) and low-( Fengyousimiao ) PAE accumulating rice cultivars affect soil PAE bioavailability, dissipation, and accumulation variation. Rice root exudates including low molecular weight organic acids (LMWOAs) of Peizataifeng and Fengyousimiao could enhance desorption of two typical PAE compounds, di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP), from aged soil to their available fractions by increasing soil dissolved organic carbon (DOC), thus improving their bioavailability in soil. Peizataifeng produced twice higher amounts of oxalic acid , critic acid and malonic acid in root exudates, and exhibited stronger effects on enhancing desorption and bioavailability of DBP and DEHP than Fengyousimiao . Higher (by about 50%) total organic carbon contents of root exudates from Peizataifeng led to higher (by 10–30%) soil microbial biomass carbon and nitrogen than Fengyousimiao , and thus promoted more PAE dissipation from soil than Fengyousimiao . Nevertheless, higher (by 20–50%) soil DOC and significantly higher PAE bioavailability in the soils planted Peizataifeng resulted in greater (by 53–93%) PAE accumulation in roots and shoots of Peizataifeng than Fengyousimiao , confirming by higher (by 1.82–3.48 folds) shoot and root bioconcentration factors of Peizataifeng than Fengyousimiao . This study reveals that the difference in root exudate extent and LMWOAs between Peizataifeng and Fengyousimiao differentiates PAE accumulation.
研究领域:
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Shuqi Liu,Yuxin Jiang,Jiancheng Sun,Fan Yang,Yuqing Wang,Yongqing Lu,Weiqiang Lai,Chao-an Long
Perillaldehyde (PAE) is a green and safe active ingredient of natural plants. The inhibitory activity of PAE against the citrus postharvest pathogen Penicillium digitatum and its ability to control green mold were evaluated by in vitro and in vivo experiments. The results revealed that the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of PAE against P. digitatum were 0.5 and 1 μL/mL, respectively. The results of conductivity, cell activity staining, and calcium fluoride white staining revealed that PAE damaged the membrane and cell wall integrity of P. digitatum . PAE promoted ROS accumulation in P. digitatum , as shown by 2',7'-Dichlorofluorescein diacetate (DCHF-DA) staining, which may induce cell death. To further investigate the molecular regulatory network of PAE in P. digitatum , we performed an RNA-sequencing analysis. The results displayed that differentially expressed genes were significantly enriched in the biogenetic pathway of ribosomes, and all of these genes were downregulated, suggesting that PAE treatment interfered with ribosome biogenesis in P. digitatum and inhibited the normal life processes of cells. Furthermore, PAE treatment reduced the incidence and severity of citrus green mold, and the overall control effect of the PAE treatment group with 5 × MFC was greater than that with 10 × MFC. Therefore, we determined the activity of enzymes related to fruit disease resistance. The results revealed that PAE treatment significantly increased the activities of CAT, POD, and PAL in citrus. These findings demonstrate that PAE prevents green mold disease by increasing the activity of citrus disease-resistant enzymes.
研究领域:
植物病理学
·
采后生物学
·
分子生物学
·
微生物学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 18
·17768-17780
·
IF 2.8
Wu Yang,Chen Xue-Xue,Zhu Ting-Kai,Li Xing,Chen Xiao-Hong,Mo Ce-Hui,Li Yan-Wen,Cai Quan-Ying,Wong Ming-Hung
The extensive use of plasticizers containing di- n -butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) results in high residual concentrations in agricultural soils and poses potential risks to human health through the food chain. Here, two rice cultivars with low ( Fengyousimiao ) and high ( Peizataifeng ) phthalic acid ester (PAE) accumulation were grown in leaching columns packed with DBP- and DEHP-contaminated soils to investigate their transport, fraction distribution, and accumulation in soil-rice-water system. Significant differences in soil vertical distribution of DBP and DEHP were observed among the two cultivars, sterilization and non-sterilization treatments . Both DBP and DEHP could leach to the bottom layer even though their concentrations in both soil and pore water decreased along with soil depth. DBP and DEHP concentrations in pore water were significantly correlated with those in corresponding soil layer at ripening stage. The available fractions including desorbing and non-desorbing fractions were predominant in the total concentrations of DBP and DEHP of soils. DBP and DEHP storages in coarse soil fractions (i.e., coarse and fine sands) with higher bioavailability displayed higher bioconcentration factors compared to finer soil fractions (i.e., silt and clay), and bioconcentration factors of Peizataifeng were higher than those of Fengyousimiao . The variations in vertical migration and accumulation of DBP and DEHP by the two cultivars implied different adverse effects on the security of groundwater and food.
研究领域:
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）