mLife
·
2022
·Vol. 1
·期 3
·287-302
·
IF 0
Lige Zhang,Huan Liu,Junbiao Dai,Ping Xu,Hongzhi Tang
Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent pollutants with adverse biological effects and pose a serious threat to ecological environments and human health. The previously isolated phenanthrene-degrading bacterial consortium (PDMC) consists of the genera Sphingobium and Pseudomonas and can degrade a wide range of PAHs. To identify the degradation mechanism of PAHs in the consortium PDMC, metagenomic binning was conducted and a Sphingomonadales assembly genome with 100% completeness was obtained. Additionally, Sphingobium sp. SHPJ-2, an efficient degrader of PAHs, was successfully isolated from the consortium PDMC. Strain SHPJ-2 has powerful degrading abilities and various degradation pathways of high-molecular-weight PAHs, including fluoranthene, pyrene, benzo[a]anthracene, and chrysene. Two ring-hydroxylating dioxygenases, five cytochrome P450s, and a pair of electron transfer chains associated with PAH degradation in strain SHPJ-2, which share 83.0%–99.0% similarity with their corresponding homologous proteins, were identified by a combination of Sphingomonadales assembly genome annotation, reverse-transcription quantitative polymerase chain reaction and heterologous expression. Furthermore, when coexpressed in Escherichia coli BL21(DE3) with the appropriate electron transfer chain, PhnA1B1 could effectively degrade chrysene and benzo[a]anthracene, while PhnA2B2 degrade fluoranthene. Altogether, these results provide a comprehensive assessment of strain SHPJ-2 and contribute to a better understanding of the molecular mechanism responsible for the PAH degradation. Impact statement Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and have attracted widespread attention due to their adverse effects. Several bacteria acquired the capability to degrade PAHs, with Sphingobium strains being the most dominant genus. However, the degradation mechanisms in Sphingobium strains that degrade high-molecular-weight PAHs are poorly understood. Here, Sphingobium sp. strain SHPJ-2, a highly efficient PAH degrader in the phenanthrene-degrading bacterial consortium, was identified and characterized. In addition, several ring-hydroxylating dioxygenases and P450s which can degrade high-molecular-weight PAHs were also identified. Our findings reveal that strain SHPJ-2 has efficient PAHs-degrading genetic resources and could help unveil the degradation mechanisms related to PAH degradation in Sphingomonads.
研究领域:
环境微生物学
·
生物修复
·
分子生物学
✓ 已验证
DOI
BIOCHEMICAL GENETICS
·
2020
·Vol. 58
·期 4
·551-565
·
IF 2.027
Xie Shaolin,Zhou Aiguo,Feng Yongyong,Zhang Yue,Li Junyi,Sun Zhuolin,Fan Lanfen,Zou Jixing
Cytochrome P4501A (CYP1A) has been used as a specific biomarker for monitoring water contamination such as PAHs, PCBs and dioxins. In the present study, the cyp1a gene of Gambusia affinis was cloned and sequenced and their expressions under PAHs exposure were characterized. The newly identified cyp1a encodes a protein with 521 amino acids that shared 96–80% identity with other Cyprinodontiformes fishes. RT-PCR analysis revealed that the basal mRNA level of cyp1a was highly expressed in liver and intestine. The expression level of cyp1a was significantly induced by exposure to 100 μg/L 3, 4-Benzopyrene (BaP) for 5 days in the muscle, testis, brain, liver and intestine of adult male fish. Except in the testis, the induced mRNA level of cyp1a ultimately decreased after prolonging the exposure time to 25 days. As for testis, the induced mRNA level of cyp1a was maintained at a high level during the entire exposure time under 100 μg/L BaP exposure. Furthermore, the expression of cyp1a increased with exposure time under a relatively low exposure concentrations 1 μg/L. Regarding the effects of other PAHs, D(a,h)A, BbF, and BaA showed a statistically significant effect of induction on mRNA level of cyp1a in the muscle, testis, brain, liver and intestine.
研究领域:
环境科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 22
·13530-13536
·
IF 3.361
Yaxiong Zeng,Ming Zhang,Daohui Lin,Kun Yang
Recycling of washing effluent by selective sorption using resins is a feasible method to lower the operation costs of surfactant enhanced remediation (SER). In this study, correlations capable of predicting the selective sorption removal of polycyclic aromatic hydrocarbons (PAHs) by resin SP850 from TX100 solution to recycle washing effluent in SER were developed. A negative relationship of sorption coefficients (log Kf) of PAHs by resin SP850 with TX100 initial concentrations (log C0,TX100) and water solubilities (log Sw) of PAHs was observed, which indicated that solubility enhancement of PAHs in TX100 micelles was responsible for the decreasing of the selective sorption. Freundlich exponential coefficients (1/n) of PAHs were relatively constant (0.775 ± 0.012), suggesting that the sorption of PAHs by SP850 in the presence of surfactant is a surface adsorption process. The modified selectivity parameter (S*), having a relationship with log C0,TX100 and PAHs log Sw as well, could be employed to evaluate the efficiency of the selective sorption process and select the optimal TX100 concentration in washing effluents. For example, at the given SP850 dose of 1.0 g L−1, the optimal TX100 concentrations (CopTX100) for naphthalene, acenaphthene, phenanthrene, pyrene, anthracene and benzanthracene were about 4200, 7100, 8000, 10 000, 18 000 and 19 500 mg L−1, respectively, having a negative relationship with their log Sw. Moreover, the CopTX100 was independent of the solid-to-solution ratio of SP850 and TX100 solution containing PAHs. These correlations would be helpful for the application of SER in contaminated soils by giving a method to quantitatively predict the selective sorption behaviors of PAHs by SP850 from TX100 solution, especially for the CopTX100, using the Sw of organic compounds and surfactant concentrations.
研究领域:
环境科学
·
化学工程
·
水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED TOXICOLOGY
·
2023
·
IF 3.3
Miao Yang,Kanmin Mao,Xin Cao,Hongjuan Liu,Xinzheng Wang,Weifeng Mao,Liping Hao
The metabolism of polycyclic aromatic hydrocarbons (PAHs) and the elimination kinetics of their mono-hydroxylated metabolites (OH-PAHs) following single exposure to different combinations of four PAHs (PAH4) were studied. Male Sprague–Dawley rats were orally exposed to a single dose of benzo[a]pyrene (B[a]P) or PAH2 (B[a]P + chrysene), PAH3 (B[a]P + chrysene + benz[a]anthracene), and PAH4 (B[a]P + chrysene + B[a]A + benzo[b]fluoranthene) with each combination adjusted to the same dose of individual compound. OH-PAHs including 3-hydroxybenzo[a]pyrene, 3-hydroxychrysene, 3-hydroxybenz[a]anthracene, and 1-hydroxypyrene (1-OHP) were detected in serum and urine samples collected at six intervals over a 72-h period post-dosing. The hepatic mRNA levels of cytochrome P450 (CYPs) were determined to ascertain the expression induction of PAHs metabolic enzymes. Results showed OH-PAHs (except 1-OHP) peaked within 8 h in serum and were excreted from urine within 24–48 h. The serum and urinary concentration of 3-hydroxybenzo[a]pyrene was significantly increased after PAH4 exposure compared with other PAHs combinations. Inversely, urinary concentration of 3-hydroxychrysene was decreased after PAH4 exposure, and the kinetics of 3-hydroxybenz[a]anthracene or 1-OHP were not different depending on the PAHs combinations. Also, CYPs were markedly induced by PAHs. Notably, the induction levels of CYP1A1 and CYP1B1 were significantly higher after PAH4 exposure compared with B[a]P exposure. The results indicated the metabolism of B[a]P was accelerated after PAH4 exposure which might be partly due to the induction of CYPs. These results confirmed PAHs are rapidly metabolized and suggested potential interactions of PAHs may happen among PAH4 mixture.
研究领域:
毒理学
·
药理学
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环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Xuemei Wang,Zhen Zhang,Jing Ru,Pengfei Huang,Yuan Ma,Jie Zhang,Xinzhen Du,Xiaoquan Lu
In view of the serious carcinogenic, teratogenic and mutagenic properties of polycyclic aromatic hydrocarbons (PAHs), which cause significant harm to human beings and the environment. Therefore, developing rapid adsorption and efficient analytical methods are research topic with practical significance. Herein, an innovative MOFs@COFs-based molecular trap materials were prepared with hierarchical porous core–shell structure and used as solid-phase microextraction (SPME) fiber coating, which combined with high performance liquid chromatography (HPLC) to achieve the efficient separation, capture, and detection for 7 PAHs in real samples. The possible adsorption mechanism was systematically verified and explained. Under the optimized conditions, the method developed in this experiment had a wide linear range (0.005–400 µg·L −1 ) with a good linear correlation coefficient (R 2 > 0.9984), low detection limits (0.002–0.405 µg·L −1 , S/N = 3) and low quantification limits (0.005–1.351 µg·L −1 , S/N = 10). The relative standard deviations of individual fibers and different batches of fibers were 0.26–3.64% and 0.40–5.91%, respectively. Besides, the fiber showed higher enrichment capacity and extraction efficiency for PAHs compared to commercial SPME fibers and other material fibers, and exhibited excellent acid-base stability and reproducibility. This work provided a favorable synthetic pathway for the preparation of MOFs@COFs composites by layer-modified assembly synthesis strategy, showed excellent practical applicability as a SPME fiber coating combined with HPLC, and could be realized to provide technical support for future studies on the separation, adsorption, and analysis of PAHs in the petrochemical industrial zone of the Yellow River Basin.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Yue Gao,Yanan Xue,Kai Zhen,Jiacheng Guo,Xuejiao Tang,Peng Zhang,Cuiping Wang,Hongwen Sun,Jizhou Wu
The remediation of soil contaminated with hydrophobic organic pollutants has attracted great public concern. In the present study, a novel catalyst using biochar supported ferro ferric oxide modified by carboxymethyl cellulose (CMC-Fe 3 O 4 /BC) was developed to activate the Fenton reaction for hazardous hydrophobic organic pollutants , and the degradation mechanisms were analyzed in terms of free radicals , electron transfer pathways and degradation intermediates. The results showed that the CMC-Fe 3 O 4 /BC-activated H 2 O 2 system degraded nearly 100% of pyrene in the aqueous system after a 1440-min reaction. The catalyst was also applied to remediate industrial field soil contaminated with PAHs and γ-HCH. The removal rate of the total pollutants reached 61.1% after a 10-day reaction, which was higher than that of Fe 3 O 4 /BC without modification. CMC enabled the Fe 3 O 4 particles to more equably distribute on the BC surface, further effectively activating H 2 O 2 to generate more ⋅OH and forming different degradation products compared to the Fe 3 O 4 /BC. Additionally, the CMC-Fe 3 O 4 /BC-activated H 2 O 2 system obviously enhanced electron transfer on the BC surface. Thus, the PAHs and γ-HCH could be degraded via electron transfer pathways.
研究领域:
环境科学
·
土壤修复
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
·
2021
·
IF 5.553
Jun Mu,Qingxue Leng,Guangfeng Yang,Baikang Zhu
Recurring oil spill accidents have been a global challenge and contribute to PAHs' heavy accumulation in marine sediments. The rapid bioremediation of PAHs with high concentrations in marine sediments has rarely been studied. In this study, four representative PAHs in crude oil were tested for fast anaerobic biodegradation. An efficient system for the anaerobic degradation of high-concentration PAHs was obtained using petroleum-acclimated marine sediments as inoculants in the treatment system. The degradation efficiencies of benzo[ b ]fluoranthene, benzo[ a ]pyrene, pyrene, and phenanthrene reached 0.21, 1.71, 3.89, and 4.10 mg/(L·d), respectively, which are 16, 2.8, 1.8, and 1.0 times higher than the reported values. Nitrate was preferred to sulfate as an electron acceptor. The acclimated sediment contains a high abundance of hydrocarbon-degrading bacteria. The number and diversity of species in the treatment system supplemented with PAHs decreased, but the abundance of some hydrocarbon-degrading bacteria and hydrocarbon-intermediate utilising bacteria increased, and ecological succession was observed.
研究领域:
环境科学
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微生物学
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生物修复
·
海洋生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Guanqing Jiang,Xu Song,Jiaying Xie,Tongxing Shi,Qiaoyuan Yang
Polycyclic aromatic hydrocarbons (PAHs) in PM 2.5 pose potentially serious threats to human health. In this study, the distribution characteristics of 16 priority controlled, fine PM (PM 2.5 )-bound PAHs in the ambient air of Guangzhou city were analysed from 2016 to 2019. Four high-molecular-weight PAHs with the highest annual average concentrations were benzo[ghi]perylene (BghiP; 0.757 ng/m 3 ), indeno(1,2,3-cd)pyrene (IcdP; 0.627 ng/m 3 ), benzo[ b ]fluoranthene (BbF, 0.519 ng/m 3 ) and 3,4-benzopyrene (BaP; 0.426 ng/m 3 ). Increasing concentrations of BghiP, IcdP, BbF and BaP were associated with increasing numbers of outpatient visits for respiratory diseases, indicating that exposure to these PAHs potentially causes acute respiratory injury in residents. Acute exposure of the human bronchial epithelial cell line BEAS-2B cells to BghiP, IcdP, BbF and BaP in vitro resulted in acute inflammation, DNA damage and apoptosis. Further bioinformatic analysis indicated that nuclear receptor subfamily 1 group D member 1 ( NR1D1 ) may be a key target gene involved in mediating the toxic effects of BghiP. Collectively, our results suggest that BghiP and the other PAHs represented by it can damage the respiratory system and induce lung cancer. This study provides valuable evidence regarding the potential health risks posed by local ambient PAHs pollution.
研究领域:
环境科学
·
公共卫生
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Chunhui Zhang,Yue Gao,Cuiping Wang,Hongwen Sun
Here, we present a first investigation of the inhibition mechanism of surfactant Triton X-100 (TX-100) on the oxidation degradation of polycyclic aromatic hydrocarbons (PAHs) in site soil aggregates using sodium citrate assisted Fe 2+ -activated persulfate (SC/Fe 2+ /PS). First, TX-100 was not only competed the adsorption sites of soil aggregates with PS, but also consumed PS, which inhibit the PAHs remediation rate in the TX-100 elution followed by the SC/Fe 2+ /PS oxidation system from 55.6% in the oxidation system to 50.3%. Furthermore, in the oxidation followed by elution system, PAHs was adsorbed on the iron minerals produced during the oxidation, which would be form a bound PAHs that was difficult to react with PS, and then re-eluted to the soil by the TX-100. Additionally, it was found that the oxidative and the elution efficiency of PAHs exhibited negative correlations with aggregate particle sizes. Finally, soil microorganism communities were more strongly changed by SC/Fe 2+ /PS oxidation and PAHs concentration than that of TX-100 elution, with obvious alterations bacteria than fungi, the effects of SC/Fe 2+ /PS and PAHs concentration on microorganism communities were opposite. This study provided a proof of regulating mechanisms for the site soil remediation using surfactants combined with the iron-PS system. Environmental Implications Surfactants may have inhibitory effects in advanced oxidative systems on the removal of PAHs and may alter soil microorganism diversity. It is necessary to comprehensively analyze the intrinsic regulatory mechanisms, and the correlation between the remediation process and soil microbial community structure of surfactants affecting the remediation efficiency of PAHs in site soil aggregates. This work comprehensively discussed the controlling mechanisms of surfactants affect the oxidation remediation of PAHs in the site soil and providing new insights for the design of the remediation technologies for the soil polluted with PAHs in the future.
研究领域:
环境科学
·
土壤化学
·
环境工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yunli Liu,Haiyang Hu,Giulio Zanaroli,Ping Xu,Hongzhi Tang
Polycyclic aromatic hydrocarbons (PAHs) and heterocyclic derivatives are organic pollutants that pose a serious health risk to human beings. In this study, a newly isolated Pseudomonas brassicacearum strain MPDS could effectively degrade PAHs and heterocyclic derivatives, including naphthalene , fluorene, dibenzofuran (DBF) and dibenzothiophene (DBT). Notably, strain MPDS is able to degrade fluorene, DBF and DBT uniquely via a lateral dioxygenation pathway, while most reported strains degrade fluorene, DBF and DBT via an angular dioxygenation pathway or co-metabolize them via a lateral dioxygenation pathway. Strain MPDS completely degraded 50 mg naphthalene (in 50 mL medium) in 84 h, and OD 600 reached 1.0–1.1; while, it stabilized at OD 600 0.5–0.6 with 5 mg fluorene or DBF or DBT. Meanwhile, 65.7% DBF and 32.1% DBT were degraded in 96 h, and 40.3% fluorene was degraded in 72 h, respectively. Through genomic and transcriptomic analyses, and comparative genomic analysis with another DBF degradation strain, relevant gene clusters were predicted, and a naphthalene-degrading gene cluster was identified. This study provides understanding of degradation of PAHs and their heterocyclic derivatives, as well as new insights into the lateral dioxygenation pathway of relevant contaminants.
研究领域:
环境微生物学
·
生物修复
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 48
·38021-38029
·
IF 3.84
Xun'an Ning,Lingzhi Shen,Jian Sun,Chaoping Lin,Yaping Zhang,Zuoyi Yang,Shujun Chen
The degradation of polycyclic aromatic hydrocarbons (PAHs) in textile dyeing sludge by O3/H2O2 treatment was investigated. Fluorene (Fl), phenanthrene (Ph), anthracene (An) and dibenz[a,h]anthracene (DBA) were selected to represent typical PAHs. The effect of radical inhibitors, H2O2, O3 flow rate, pH and reaction time on the degradation of the four types of PAHs were examined to determine the optimal conditions for degradation and to clarify the mechanism(s) of PAHs degradation. The results indicated that the combined O3/H2O2 treatment resulted in high removal rates of the selected PAHs: 89% for Fl, 66% for Ph, 71% for An and 81% for DBA. These results correspond to the optimal conditions of an O3 flow rate of 0.4 L min−1, a H2O2 concentration of 0.60 mol L−1, a reaction time of 15 min and pH 7. The removal rate (within 30 min) increased by 5% for Fl, 27% for Ph, 21% for An and 5% for DBA through the addition of H2O2 to the O3 process. The O3 flow rate, reaction time and pH were the key parameters affecting PAH degradation with multiple interacting factors (p < 0.05) by the O3/H2O2 treatment. The degradation of the PAHs can be fitted well by pseudo-first-order kinetics (R2 = 0.9656). The combined O3/H2O2 treatment is demonstrated to be an efficient approach for the degradation of PAHs in textile dyeing sludge.
研究领域:
环境工程
·
化学工程
·
废水处理
✓ 已验证
产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2024
·
IF 5.6
Yuke Hou,Yangjian Hu,Min Li,Jiahui Nong,Fengyuan Xie,Yuhan Fan,Jianhao Zhang,Xianming Zeng,Minyi Han,Xinglian Xu,Xia Wang
The cold plasma (CP) technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon (PAH) in this investigation. Two different CP treatments methods were implemented in the production of beef patties, to investigate their inhibition and degradation capacity on PAHs. With 5 different cooking oils and fats addition, the inhibition mechanism of in-package cold plasma (ICP) pretreatment was explored from the aspect of raw patties fatty acids composition variation. The results of principal component analysis showed that the first two principal components accounted for more than 80 % of the total variation in the original data, indicating that the content of saturated fatty acids was significantly positively correlated with the formation of PAHs. ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids, which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased. Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls, utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality. In conclusion, CP treatment degraded PAHs through stepwise ring-opening oxidation in 2 reported pathways, the toxicity of PAHs contaminated products was alleviated after CP treatment.
研究领域:
食品科学
·
环境化学
·
等离子技术
·
肉类加工
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）