JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Zhaohui Guo,Christian M. Pedersen,Pengfei Wang,Minjun Ma,Yingqing Zhao,Yan Qiao,Yingxiong Wang
The isomerization of d-glucose to d-fructose plays a key role in the biochemical and chemical conversion of biomass, and it is therefore desirable to develop and improve catalysts for this reaction. In this study, the environmentally friendly polymer poly(amidoamine) (PAMAM) dendrimer’s properties as catalysts for this isomerization are investigated. The experimental results showed that the PAMAM dendrimers, which have basic terminal groups, can effectively promote the d-glucose isomerization reaction. Under the optimized reaction conditions, d-fructose was generated with a 20% maximum yield and above 90% selectivity. 13C and 2H isotope experiments by NMR were carried out to explore the reaction mechanism. When the reaction was performed in D2O, the C1 signal of d-fructose changed to a triplet, which confirmed that the C1 carbon binds to a deuterium atom, i.e., isotopic exchange. It was also found that the deuterium atom at the C2 position of d-glucose-2-d1 cannot transfer to d-fructose. These data indicate that PAMAM dendrimers catalyze d-glucose isomerization through a mechanism, which includes deprotonation, formation of ene-diol intermediate, and proton exchange with the solvent.
研究领域:
生物化学
·
绿色化学
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Caifang Ning,Hui Ma,Christian Marcus Pedersen,Hui Chang,Yingxiong Wang,Yan Qiao
Perfluorooctanoic acid (PFOA) is a persistent environmental contaminant with serious negative health effects, and removal of it from water is therefore highly relevant for environmental safety. In this paper, poly(amidoamine) (PAMAM) dendrimers have been proposed as hosts for the PFOA in order to remove it from aqueous solutions. The different aggregate structures for PAMAM-PFOA complexes with increase of −COO − /−NH 3 + molar ratios were proposed on the basis NMR titrations using 19 F NMR and further analyzed by the relaxation and diffusion measurements. It is suggested that PFOA micelles are transferred to the bilayer with increasing PAMAM concentration. Moreover, the protonated surface amine groups of PAMAM contribute to reduce the critical aggregation concentration (CAC) of PFOA. The study shows that the main driving force for interaction between PAMAM and PFOA is ionic interaction. This research will serve as a starting-point for removing the fluorinated contamination by using PAMAM dendrimers.
研究领域:
环境科学
·
化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuai Zhang,Fuxiong Ren,Kailei Wang,Yuan Gao,Yujia Lu,Junping Han,Li Chen,Haoyun Wang,Yiping Zhao
The rapid development of industries has intensified the issue of oily wastewater pollution, necessitating sustainable solutions. Biomass aerogels, known for their environmental friendliness and biocompatibility, offer promising prospects for oil–water separation. This study fabricated a polyamidoamine (PAMAM)-modified chitosan/cellulose interpenetrating polymer network aerogel via sequential cross-linking and directional freeze-drying. This method endowed the aerogel with excellent mechanical properties, including good strength at a low density (0.06–0.11 g/cm 3 ), high anisotropy at 80 % strain, a high specific surface area (1.93–12.56 m 2 /g), and hydrophobicity (WCA = 139.5°). The aerogel exhibited outstanding separation efficiencies for the carbon tetrachloride/water mixture (5501.85 L·m −2 ·h −1 ) and water-in-oil emulsions (4198.60 L·m −2 ·h −1 , 96.67 %), as well as a removal rate of 61.24 % for the cationic dye RhB. A “demulsification–adsorption synergistic separation” mechanism involving dendritic PAMAM polymers and hydrophobic functional groups was proposed, with performance initially increasing and then decreasing as the PAMAM generation number increased. Despite certain limitations, such as sensitivity to environmental factors, the prepared all-biomass aerogel offered a green, efficient, and mechanically robust solution for the treatment of oily wastewater. This study provided a sustainable strategy for fabricating multifunctional hydrophobic aerogels, paving the way for advanced applications in environmental remediation.
研究领域:
材料科学
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环境科学
·
生物工程
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2016
·
IF 3.773
XiuLing Xu,JingJing Li,ShunPing Han,ChengHao Tao,Luo Fang,Yue Sun,JiaZhen Zhu,ZeHua Liang,FanZhu Li
Effective targeting drug delivery system for glioma treatment is still greatly challenged by the existence of the blood–brain barrier (BBB) and the intracranial overspreading of anti-tumor drug. Herein, we presented a dual-functional glioma targeting delivery of doxorubicin based on the PAMAM G5 dendrimer , modified with folic acid (FA) to target tumor cell, also borneol (BO), a well known safe material derived from traditional Chinese medicine , to facilitate the BBB permeability and reduce the toxicity of naked PAMAM. The intracranial transportation and glioma targeting ability were evaluated on the BBB model and C6 glioma cells in vitro . Also, pharmacokinetics and biodistribution were studied on C6 glioma-bearing rats in vivo . It indeed reduced the cytotoxicity of PAMAM against both HBMEC and C6 cells by coupling BO on the surface, while efficiently boosted BBB permeability with the improvement of transportation ratio by 2 folds to the BO-unmodified conjugates. Furthermore, conjugated FA increased total uptake amount by C6 cells leading to strong inhibition with the 3-fold lower IC 50 value than FA-unmodified DOX conjugate. In comparison with DOX solution, FA-BO-PAMAM/DOX exhibited significantly prolonged half-life time and increased area under the curve and improved DOX accumulation in brain tumor. The tumor growth inhibition, in vivo , was significantly increased up to 57.4%. The median survival time of xenograft rats after administering FA-BO-PAMAM/DOX (28 days) was significantly prolonged compared to free DOX (18 days, P < 0.05) or other controls. In conclusion, this strategy of novel targeting nanocarrier provides a promising method to increase the drug accumulation in the tumor site for therapy of glioma.
研究领域:
药理学
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肿瘤学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Huimin Li,Xinru Yuan,Mei Ren,Jiajia Liu,Yixin Zheng,Ziyi Lin,Zixin Chen,Zhao Yang,Xin Su,Heyun Shen
Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/ref-1, APE1), a vital protein for DNA repair and cellular redox regulation, is frequently overexpressed in tumor cells, underscoring the importance of developing sensitive detection methods for early cancer diagnosis. However, the rapid detection and visualization of nuclear APE1 in tumor cells are still challenging. In this study, we successfully developed a novel DNA fluorescent nanoprobe based on polyamidoamine (PAMAM) for the rapid detection of cytoplasmic and nuclear APE1. The PAMAM surface was modified with arginine (Arg), named PR, and its hydrophobic core encapsulated the 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride (TA) dye to construct fluorescent nanoparticles (TPR). Furthermore, an APE1-responsive dsDNA (SP) was linked on the TPR surface, containing apurinic/apyrimidinic sites (AP sites) and the black hole quencher 2 (BHQ2) and ensuring that fluorescence remains off in the absence of APE1. TPR-SP exhibited a detection range of 0.125–25 U mL–1 and a detection limit as low as 0.03 U mL–1. Compared with the Arg-free nanoprobes (TP-SP), TPR-SP significantly accelerated endocytosis and nuclear penetration, reducing the APE1 detection time to one-quarter (from 2 to 0.5 h). Notably, the APE1 signal in the whole nucleus can also be significantly detected. Thus, the TPR nanoprobe achieves the rapid enrichment and amplification of fluorescence signals, leading to highly sensitive whole cellular APE1 detection. This innovative and efficient detection method greatly expands the technological means for early cancer detection.
研究领域:
生物化学
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分子生物学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 55
·34754-34765
·
IF 3.361
Wei Qin,Kaixuan Xu,Junwei Wang,Xiaofeng Cui,Jianli Zhang,Yaqing Weng
To overcome the urgency of zirconium and hafnium separation, a novel mesoporous silica sorbent (PS-G1.0-MSNs) modified with phosphorous-functionalized G1.0 PAMAM dendrimers was prepared. The adsorption and separation behaviors of PS-G1.0-MSNs adsorbent on Zr(IV) and Hf(IV) were perfromed as a function of acidity, contact time, temperature, and ion concentrations by batch sorption methods. The maximum adsorption capacities for Zr(IV) and Hf(IV) were 25.7 mg g−1 and 5.36 mg g−1 under optimal experimental conditions, respectively, and the separation factor βHf/Zr = 2.0 > 1 demonstrated that the prepared sorbent had preferential selectivity for Hf(IV) in rich Zr(IV) solution. Moreover, kinetic data indicated that the sorption process on Zr(IV) and Hf(IV) achieved equilibrium within 120 min, and followed the pseudo-first-order model with a rate-determining step. The adsorption amount increased as temperature raised from 283 K to 303 K and the isothermal data plotted with the Langmuir model was better than the Freundlich model with monolayer behavior. Thermodynamic data analysis indicated that the sorption process was spontaneous and endothermic. Furthermore, XPS analysis revealed that the metal ion adsorption was mainly induced by the chemical coordination of Zr(IV) and Hf(IV) ions with N, O, P atoms of amide and phosphate groups. The present work provides good guidelines on the design of high efficient sorbent for the separation of Hf(IV) from Zr(IV) solutions.
研究领域:
材料科学
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化学工程
·
环境科学
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Yanshuai Cui,Bo Liang,Longgang Wang,Linlin Zhu,Jianxin Kang,Haotian Sun,Shengfu Chen
The surface charge of dendrimers is one of the key factors that determine their use in nanomedicine. Generation 5 poly(amido amine) dendrimers (G5 PAMAM) encapsulating with fluorescein sodium were employed to study the method to tune surface charge. Firstly, the surface primary amines were reacted with maleic anhydride to introduce double bonds. Then, l -cysteine and cysteamine were conjugated to these double bonds via thiol-ene additions in water, respectively. The surface charges of modified G5 PAMAMs were successfully controlled by tuning the molar ratio of L-cysteine to cysteamine. The surface charges of the resulting modified G5 PAMAMs varied from −16.0 mV to −3.7 mV at physiological pH. In addition, they showed good compatibility with proteins and cells compared with G5 PAMAM. Modified G5 PAMAMs and fibrinogen could coexist in solution without generating noticeable aggregation, while G5 PAMAM induces significant aggregation, indicating these modifications can effectively reduce the interaction force between G5 PAMAM and proteins. Furthermore, modified G5 PAMAMs exhibited negligible hemolysis, while G5 PAMAM caused severe hemolysis. The cytotoxicity assay demonstrated that modified G5 PAMAMs exhibited very low cytotoxicity to both HUVEC cells and KB cells (>90% cell viability) at high concentrations up to 2 mg/mL. The cellular uptake of them was much less efficient compared with that of G5 PAMAM. Moreover, the intravenous injected modified G5 PAMAMs were excreted by kidney with a relatively little accumulation in liver, confirming their good biocompatibility in vivo . It is expected that the modified G5 PAMAMs could be an excellent candidate for contrast agent carriers in the future.
研究领域:
纳米医学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Longgang Wang,Yanshuai Cui,Shengfu Chen,Guangzhi Wang,Dawei Gao,Yanping Liu,Qiaoyue Luo,Zhiwei Liu,Xuwu Zhang
The dendrimer based synthetic mimetic enzyme has been drawing great attention. However, this mimetic enzyme is different from the natural enzymes, which are pH sensitive, biocompatible and keep their catalytic activity in biological complex medium. A single zwitterionic layer composed by primary amine and carboxyl groups may be a useful method to obtain these properties. Herein, we report a novel facile method to prepare a mimetic enzyme. The complexes of generation 5 poly(amido amine) dendrimers (G5 PAMAM) with free hemin (G5Hs) were modified by the maleic anhydride and cysteamine. Results showed that the mimetic enzymes (G5HMCs) had pH sensitivity and good stability by varying the pH from 4 to 9, while significant precipitation was observed for free hemin at pH 5 after two days. The G5HMC (3:1) showed optimal catalytic activity at its isoelectric point. Furthermore, G5HMCs displayed excellent biocompatibility. The G5HMCs incubated with fibrinogen were stable for 24 h, while G5Hs immediately formed large aggregates. G5HMC (3:1 2 mg/mL) displayed little cytotoxicity with HeLa cells or A549 cells for 24 h, while G5H (3:1) had serious cytotoxicity, which was also demonstrated by cell morphology observation. At last, G5HMCs fully preserved their catalytic activity in bovine serum albumin (BSA) solution compared with phosphate buffer saline (PBS) solution, while hemin decreased to 73.5–81.5% catalytic activity in BSA solution, which was caused by the less interaction with BSA for G5HMCs than free hemin. The surface functionalization schemes described in this report would represent a versatile method to prepare water-soluble, pH sensitive, biocompatible, and efficient artificial enzymes for biomedical related applications.
研究领域:
生物化学
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纳米医学
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材料科学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2011
·
IF 4.304
Lihong Zhang,Saijie Zhu,Lili Qian,Yuanying Pei,Yongming Qiu,Yanyan Jiang
This work was based on our recent studies that a promising conjugate, RGD-modified PEGylated polyamidoamine (PAMAM) dendrimer with doxorubicin (DOX) conjugated by acid-sensitive cis -aconityl linkage (RGD-PPCD), could increase tumor targeting by binding with the integrin receptors overexpressed on tumor cells and control release of free DOX in weakly acidic lysosomes. To explore the application of RGD-PPCD to glioma therapy, the effects of the conjugate were further evaluated in glioma model. For comparative studies, DOX was also conjugated to PEG–PAMAM by acid-insensitive succinic linkage to produce the PPSD conjugates, which was further modified by RGD to form RGD-PPSD. In vitro cytotoxicity of the acid-sensitive conjugates against C6 cells was higher than that of the acid-insensitive ones, and further the modification of RGD enhanced the cytotoxicity of the DOX-polymer conjugates as a result of the increased cellular uptake of the RGD-modified conjugates by C6 cells. In vivo pharmacokinetics, biodistribution and antitumor activity were investigated in an orthotopic murine model of C6 glioma by i.v. administration of DOX-polymer conjugates. In comparison with DOX solution, all the conjugates showed significantly prolonged half-life and increased AUC and exhibited higher accumulation in brain tumor than normal brain tissue. Although RGD-PPCD was more than 2-fold lower tumor accumulation than RGD-PPSD, it exhibited the longest survival times among all treatment groups, and therefore, RGD-PPCD conjugate provide a desirable candidate for targeted therapy of glioma.
研究领域:
药理学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Yuan Gu,Yanjun Cai,Yige Kou,Erzhuo Cheng,Hanjie Bi,Min Hu,Songyao Wu,Yong Jiang,Jingxi Zhang,Qingting Wu,Yunyi Li,Bin Yang
Nanomaterial-based drug delivery systems have demonstrated powerful applications in tumor therapy. PAMAM dendrimers by virtue of multivalent modification and biocompatible components were widely used to construction multifunctional targeted chemotherapeutics. In this report, we present a PAMAM dendrimer-based nano-prodrug delivery system for targeted tumor therapy. The RGDyC-PEG-PAMAM-PBA-Res (RPPPR) nano-prodrug was characterized by 1 H NMR, zetasizer, and TEM, the drug loading and pH-sensitive drug release behaviors were investigated. In vitro cell viability assay revealed that the group treated with the nano-prodrug exhibited lower cell viability compared to the control group as concentration increased, confirming the nano-prodrug’s effectiveness in treating tumors. In vitro studies on cellular uptake of the nano-prodrug demonstrated that RPPPR had a high targeting ability, primarily attributed to the RGD-mediated target effect. Relevant data from our hemolysis assay indicated that the nano-prodrug maintained good blood compatibility within the concentration range of 0.1 to 0.3 mg/mL. In vivo experiments focusing on tumor therapy showed that RPPPR suppressed tumor growth and promoted tumor apoptosis. H&E staining, TUNEL assay, and immunohistochemistry results further validated the enhanced therapeutic effect. Importantly, no toxicity was observed in the vital organs such as the heart, liver, lung, kidneys, or spleen. These results suggest the superior antitumor efficacy of RPPPR compared to Resveratrol alone, and thus may be useful for tumor therapy. It is expected to explore new directions to establish a general strategy with dendritic prodrugs for synergistic tumor targeting therapy.
研究领域:
纳米技术
·
药学
·
癌症治疗
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·Vol. 32
·期 7
·3326-3341
·
IF 4.7
Wang Rui,Song Xin,Liu Li,Zhou Chao,Wu Guangfeng
Magnetic particles with active groups on their surfaces can be applied to wastewater purification to remove harmful ions, but the insufficient number of active groups on their surfaces tends to limit their removal rate of ions. Herein, we grafted different generations of dendritic macromolecular polyamidoamine (PAMAM) onto the magnetic chitosan microspheres (MCS) using ethylenediamine tetraacetic acid (EDTA) as core (MCEPs) for the removal of Cu (II). Firstly, 7 μm-sized MCS with good dispersibility was prepared by adjusting conditions such as glacial acetic acid content, emulsifier content and raw material ratio. Secondly, the effect of MCEPs generation, pH and contact time on the adsorption capacity were investigated. The adsorption experiments showed that the MCEPs had a high adsorption capacity, when the adsorbent was the second generation of MCEP (MCEP G2.0), the maximum adsorption capacity can up to 702.9 mg/g and the adsorption process can be completed in 60 min. Moreover, among various isotherms models, MCEP G2.0 was well-fitted by Langmuir adsorption isotherm. Also, adsorption kinetic studies proved that the adsorption mechanism of MCEP G2.0 adsorbent followed the pseudo-second-order kinetic model, demonstrating that the process was chemisorption. The adsorbent has renewability as well, after five adsorption cycles, its adsorption efficiency was still higher than 80%. Therefore, the adsorbent showed a superior adsorption capacity and renewability to remove Cu (II). Graphical
研究领域:
环境科学
·
材料科学
·
化学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 34
·4259-4266
·
IF 0
Longgang Wang,Qinghua Yang,Guanglong Ma,Weifeng Lin,Zhen Wang,Mei Huang,Shengfu Chen
The dendrimer based synthetic enzyme mimic, so-called ‘dendrizyme’, has been of great interest since the early days of dendrimers. However, there is a lack of an effective way to obtain a natural enzyme mimic showing both good biocompatibility and high preservation of catalytic activity in biological complex medium simultaneously. Here we report a novel approach – the synthesis of a generation five dendrimer of poly(amido amine) (PAMAM) incorporating hemin through capping with carboxybetaine acrylamide (CBAA), which could function as peroxidase. Results showed that the nanocapsules of hemin using CBAA-modified PAMAM dendrimers (CBAA-H-PAMAM) exhibited excellent biocompatibility and full preservation of catalytic activity in bovine serum albumin (BSA) solution, compared with free hemin. Results indicated that the ultra-thin shell of zwitterionic CBAA groups reduced nonspecific interaction with proteins while it did not cause any obvious rise in hindrance to mass transfer. Furthermore, the synthetic peroxidase mimic (CBAA-H-PAMAM) exhibited remarkable temperature endurance as compared with natural proteins. Taken together, our results indicate that protein surface mimicking through CBAA attachment might open a new route for synthetic enzymes in biomedical related applications.
研究领域:
生物化学
·
材料科学
·
医学工程
✓ 已验证
DOI
PubMed
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