Analytical Methods
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2025
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IF 2.6
Jinran Chen,Xiurui Ke,Zhou Zhou,Wenqi Ye,Hong Liu,Wei Zhang,Xiaohong Liu
We report the fabrication of a substrate with cavity-nanorods and decorated with Ag-nanoplates (C-NR@Ag). The cavities on the substrate are formed by metal assistant chemical etching, and the Ag-nanoplates in the cavities by galvanic cell deposition enhance the SERS performance effectively. Analytes in solution are adsorbed on Ag-nanoplates and located in hot spots, which enhance the SERS performance effectively. The enhancement factor of the Ag-nanoplates decorated on nanorod cavities is calculated to be 3.6 × 106, which is about 3 fold higher than that on the nanorods. The C-NR@Ag substrate is able to detect polychlorinated biphenyls (PCBs) with the lower limit of detection at 1.0 × 10−12 M. Additionally, due to the semi-volatile nature of PCB-77, the lower limit of detection of the C-NR@Ag substrate for PCB-77 was 1.0 × 10−11 M by the non-contact collection method. These results present a novel approach towards enhancing SERS performance and facilitating the rapid detection of PCB-77.
研究领域:
材料科学
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环境科学
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分析化学
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DOI
PubMed
高影响因子
RSC Advances
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2021
·Vol. 11
·期 10
·5506-5511
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IF 3.361
Lin Yuan,Qiang Fu,Maojuan Zhou,Yunqian Ma,Lihua Zang,Yingjian Qin,Dandan Ji,Fengshan Zhang
Polychlorinated biphenyls (PCBs) are synthetic organic compounds that are extremely difficult to break down in water and can accumulate in human fat and organisms. However, methods that can be used to detect large amounts of PCBs remain unsatisfactory, as they are generally overly sensitive and involve complex operations. An aptamer-based catalytic hairpin assembly (aptamer-CHA) reaction for the selective detection of 3,3′,4,4′-tetrachlorobiphenyl (PCB 77) was developed. It combines the advantages of aptamers and signal amplification reactions. The aptamer selectivity recognizes the target, PCB 77, which triggers the sensitive CHA reaction to produce a fluorescence signal. CHA is a sensitive enzyme-free signal amplification method suitable for on-site detection. Therefore, the identification aptamer is the basis for the quantitative detection of PCB 77, with a detection range of 0.01 μg L−1 to 500 μg L−1 and a detection limit of 0.01 μg L−1. In this study, the aptamer was used to improve the selectivity of the reaction, and the CHA reaction improved the sensitivity of the detection system. Such high-sensitivity PCB detection capabilities with simplified procedures may be useful for real-time field detection and other monitoring tasks. This method can be used as a rapid fluorescence detection strategy for other targets in aquatic environments.
研究领域:
环境科学
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Wenchao Jia,Xiangying Jin,Yuhua Wu,Danping Xie,Wenhua Yin,Bo Zhao,Zhonghui Huang,Lijun Liu,Yanyan Yang,Tonghui Cao,Xidan Feng,Sheng Chang
Here, we report a novel aptasensor based on decahedral silver nanoparticles (Ag 10 NPs) enhanced fluorescence polarization (FP) for detecting PCB-77. Using aptamer modified Ag 10 NPs hybridized with DNA sequence labeled fluorescent group as an analytical probe, polychlorinated biphenyls (PCB-77) could be detected with high sensitivity and selectivity. The linear range of determination was 0.02 ng/L to 390 ng/L and the limit of detection was 5 pg/L. In addition, through the optimization of the experiment condition and signal probe DNA (pDNA), we found that the maximum FP signal could be generated when the distance between fluorescein and the surface of Ag 10 NPs was 3 nm. When the aptamer was immobilized on the surface of Ag 10 NPs could be strengthened the anti-interference performance of aptamer nanoprobe and further improved the detection ability. At the same time, we also compared the detection performance of the traditional FP signal enhancer streptavidin (SA) analysis system. The fluorescence polarization aptasensor could detect PCB-77 samples efficiently in complex environmental water, which shows a good application prospect.
研究领域:
分析化学
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环境科学
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纳米技术
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DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
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2021
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IF 4.094
Chuhong Zhu,Qiangsheng Zhao,Dexian Huo,Xiaoye Hu,Xiujuan Wang
We report a simple electrodeposition strategy to fabricate highly surface-roughened Au nanostructure arrays grown on a silicon wafer . Finite element method simulation reveals that lots of hot spots were generated around the tips of nanothorns and in the gaps between neighboring nanothorns by both plasmon coupling and ‘‘lightning-rod’’ effects. It was found that roughening the as-electrodeposited Au nanocones by a subsequent second-step electrodeposition induced a six times improvement in SERS intensity. The peak intensities of SERS spectra show a narrow distribution with a small relative standard deviation of 6.6%, confirming the high uniformity of the fabricated thorny Au nanostructure array SERS substrate. A high SERS sensitivity of the fabricated thorny Au nanostructure arrays was confirmed by a detectable concentration limit of 1 pM rhodamine 6G aqueous solutions. The SERS substrate also showed a high detection sensitivity toward ferbam with a limit of detection of about 2.40 nM. Calibration curves with a linear correlation of logarithmic SERS intensity with logarithmic concentration for R6G and 3,3′,4,4′-tetrachlorobiphenyl (PCB-77, a persistent organic pollutant) were achieved by using the fabricated rough gold nanoarrays, demonstrating a promising application in semi-quantitative or even quantitative detection. This work offered a facile but effective approach toward the rational designs of uniform, reproducible and sensitive SERS substrates for molecular detection.
研究领域:
材料科学
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纳米技术
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分析化学
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表面科学
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产品关联
DOI
高影响因子
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