Coucong Gong,Yuan Shen,Jingyi Chen,Yonggui Song,Shuiliang Chen,Yonghai Song,Li Wang
In this work, a novel hydrogen peroxide (H 2 O 2 ) biosensor was developed based on the microperoxidase-11 (MP-11) encapsulated in metal-organic frameworks, PCN-333 (Al) (PCN stands for porous coordination network), which was grown on the three-dimensional (3D) kenaf stem-derived porous carbon (3D-KSC) firmly and uniformly. The ball-flower-like PCN-333 (Al) with mesopores of 5.5 nm was especially suitable for encapsulating MP-11 (1.1 × 1.7 × 3.3 nm). Scanning electron microscopy and energy dispersive X-ray spectroscopy were used to characterize the 3D-KSC electrode and the 3D-KSC/PCN-333 (Al)@MP-11 electrode. UV–vis spectroscopy was used for characterizing the encapsulation of MP-11 in the 3D-KSC/PCN-333 (Al) electrode. Cyclic voltammetry and differential pulse voltammetry were used to characterize the electrochemical behaviors and performance of the biosensor. The results showed that the encapsulated MP-11 molecules showed a better performance than free enzyme. Under the optimized experimental conditions, the resulted H 2 O 2 biosensor exhibited a wide linear range from 0.387 μM to 1.725 mM and a low detection limit of 0.127 μM with good stability and high selectivity, which was superior to some H 2 O 2 biosensors.
In this work, a novel H 2 O 2 biosensor was developed based on the composites of microperoxidase-11 (MP-11) encapsulated in graphene oxide (GO)-PCN-333 (Al) (PCN stands for porous coordination network, one of the metal-organic frameworks). The PCN-333(Al) firmly and uniformly grew on the surface of GO sheet with large specific surface and excellent electrical conductivity, which provided a lots of pores for the encapsulation of MP-11 in the composites and selectively accumulated analytes into its mesopores to improve the selectivity of enzymatic reaction. The MP-11 molecules might freely move through mesopores of PCN-333 (Al) to maintain bioactivity, which remarkably enhanced the mass transfer rate and quickened the electron transfer for electrochemical biosensors. Scanning electron microscopy was used to characterize the GO and PCN-333(Al)-GO composites. Cyclic voltammetry and differential pulse voltammetry were used to characterize the electrochemical behaviors and performance of the H 2 O 2 biosensor. The target electrode showed excellent performance with a wide linear range from 10 μ M to 800 μ M, a low detection limit of 3 μ M, good stability and high selectivity, which was superior to some other H 2 O 2 biosensors.
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