Guoliang Zhang,Yaolong Zhang,Yu Zhang,Changshun Wang,Jin Tan,Ruxangul Jamal,Tursun Abdiryim,Ahmat Ali
Hydroxyl-functionalized MWCNs and PEDOT are better suited for loading MIP. • The sensor detects pefloxacin at the picomolar level. • Synthesis of ultra-thin tubular PEDOT-OH@Au/MWCNs-OH nanocomposites. • The excellent thin-layered structure offers an efficient electron transport pathway. To safeguard public health and prevent the increase of bacterial resistance, the creation of practical and accurate detection methods for the quantitative analysis of fluoroquinolone antibiotics is essential. In this study, a tubular PEDOT-OH@Au/MWCNTs-OH substrate material was prepared using a one-step in situ oxidation method, with chloroauric acid as the oxidant. Pyrrole (Py) was used as the functional monomer, and pefloxacin (PFX) as the template molecule, to ingeniously design a molecularly imprinted electrochemical sensor (MIP/PEDOT-OH@Au/MWCNT-OH) with sensitive sensing capabilities. As expected, the unique structure and properties of PEDOT-OH@Au/MWCNTs-OH provided the sensor more surface area and enhanced electrochemical activity, increasing the number of distinct recognition sites. Based on experimental results, the MIP/PEDOT-OH@Au/MWCNT-OH sensor showed good anti-interference and stability, a low detection limit of 0.149 nM, S/N = 3, and a broad linear range of 0.002–30 μM. Additionally, the sensor achieved a recovery rate of 97%–103% in detecting PFX in pefloxacin mesylate ointment. This sensor shows promise as a highly selective and sensitive portable detection method for the quantitative analysis of PFX in the environmental monitoring. Download: Download high-res image (239KB) Download: Download full-size image
Ao Zhuang,Yongjun Bian,Jianwei Zhou,Suna Fan,Huili Shao,Xuechao Hu,Bo Zhu,Yaopeng Zhang
Various attractive materials are being used in bioelectronics recently. In this paper, hydroxymethyl-3,4-ethylenedioxythiophene (EDOT-OH) has been in situ integrated and polymerized on the surface of the regenerated silk fibroin (RSF) film to construct a biocompatible electrode. In order to improve the efficiency of in situ polymerization, sodium dodecyl sulfate (SDS) was adopted as surfactant to construct a well-organized and stable poly(hydroxymethyl-3,4-ethylenedioxythiophene) (PEDOT-OH) coating, whereas ammonium persulfate was used as oxidant. The effects of dosages of surfactant and oxidant, initial pH value, and monomer concentration on the polymerization were studied. Under the optimal conditions, the RSF/PEDOT-OH film exhibited a square resistance of 3.28 × 105 Ω corresponding to a conductance of 6.1 × 10–3 S/cm. Scanning electron microscope images indicated that PEDOT-OH was deposited uniformly on the surface of the RSF film with SDS. Furthermore, Fourier transform infrared spectroscopy confirmed that interactions existed between the peptide linkages of silk fibroin (SF) macromolecules and PEDOT-OH. The RSF/PEDOT-OH film displayed favorable electrochemical stability, biocompatibility, and fastness. This study provides a feasible method to endow conductivity to RSF materials in various forms. In addition, the conductive layer and biocompatible silk substrate make the RSF/PEDOT-OH biomaterial highly suitable for potential applications in bioelectric devices, sensors, and tissue engineering.
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