Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 27
·5478-5489
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IF 4.726
Yong Sun,Yaning Wang,Yani Cui,Wen Zou,Yanfei Tan,Jie Liang,Yujiang Fan,Xingdong Zhang
Stimuli-responsive targeted polymeric micelles as drug delivery systems have recently attracted significant attention for the treatment of various cancers, which could improve delivery efficiency by tumor-specific recognition via active targeting strategies. In this research, DOX-incorporated bioreducible polymeric micelles based on PAA-g-PEG/PEG–Fa conjugated polymers were prepared and characterized as nanocarriers for promoting intracellular anti-tumor drug delivery efficiency via folate receptor-mediated endocytosis. The anti-proliferative activity on cancer cells, biodistribution, active-/passive-targeting efficiency, tumor growth inhibition efficiency, and biological toxicity were evaluated in vitro and in vivo. The MTT assay demonstrated that the DOX-encapsulated active-targeting PAA-g-PEG/PEG–Fa micelles had much greater growth inhibition effect against 4T1 and KB than passive-targeting micelles. These active-targeting micelles showed superior tumor accumulation and excellent tumor growth inhibition effect as revealed by the fluorescence optical imaging technique and tumor volume change investigation, as well as the survival study of the tumor-bearing Balb/c mice. Furthermore, the active targeting micelles greatly decreased the toxicity of DOX on the heart and other organs. These potential results encouraged us to further optimize the molecular structure to achieve more excellent targeted therapeutic effect.
研究领域:
药剂学
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肿瘤学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2020
·
IF 3.616
Meifeng Liu,Weichi Chen,Xingyu Zhang,Pengwen Su,Feng Yue,Shaoqun Zeng,Song Du
Madecassoside (MA) exhibits excellent therapeutic effects in wound healing and scar management. However, its high hydrophilic nature and low permeability through skin tissue limits its topical application. Liposomes are widely used to deliver drugs due to their high structural similarity and biocompatibility with cell membranes. However, normal liposome formulations are too fluid to maintain sufficient adhesion to the wound surface. In this study, in order to make an MA formulation conducive to topical administration, poly(ethylene glycol)-poly( ε -caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE), a biodegradable and temperature-responsive copolymer material, was synthesized and applied to improve the adhesion properties of MA liposomes. The mean particle size of the PECE-modified MA liposomes was 213.43±4.68 nm, and the zeta potential was −23.80±15.37 mV under the optimal conditions of EPC (egg yolk lecithin) to PECE at a mass ratio of 1:1. Additionally, PECE-modified MA liposomes maintained a hydrogel state for better adhesion until the temperature reached 43°C. Furthermore, the PECE-modified MA liposomes showed superior wound contraction effects relative to the MA liposomes in second-degree burn experiments using a rat model. These results indicated that PECE-modified MA liposomes have better surface adhesion performance and healing effects than unmodified MA liposomes.
研究领域:
药剂学
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材料科学
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生物医学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2012
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IF 3.35
Cheng Zhang,Guodong Li,Yihan Wang,Fuying Cui,Jien Zhang,Qingshan Huang
In this work, 5-fluorouracil-loaded- poly( l -lactic)-polyethylene glycol/polyethylene glycol (5-FU-loaded-PLLA–PEG/PEG) nanoparticles were prepared using a novel reverse emulsion-solution enhanced dispersion by supercritical fluids (reverse emulsion-SEDS) technique in an effort to obtain an efficient drug delivery system . In the experiment, 5-FU and PEG were dissolved in water PLLA–PEG was dissolved in organic solution, the aqueous solution was added dropwise to the organic solution under magnetic stirring, a reverse emulsion was immediately formed. The reverse emulsion was dried by a SEDS process so that 5-FU-loaded-PLLA–PEG/PEG nanoparticles (5-FU-NPs) were obtained. The particle size, size distribution, surface morphology, and physical and chemical properties of the 5-FU-NPs were investigated by laser diffraction particle size analysis, scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and thermogravimetric analysis (TGA). The drug encapsulation efficiency (EE), drug loading (DL), in vitro release profile and pharmacokinetics of 5-FU-NPs in rat plasma were investigated by high-performance liquid chromatography (HPLC). The in vivo tumor inhibition effect, increase in lifespan and hepatotoxicity of placebo-NPs, 5-FU and 5-FU-NPs were determined using H22 tumor-bearing ICR mice. These results collectively suggest that 5-FU-NPs prepared using SEDS have potential anti-tumor applications as a controlled drug release dosage form without harmful drug toxicity .
研究领域:
药剂学
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生物医学工程
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
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2012
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IF 2.625
Chi Ma,Di Pang,Zuochun Xiong,Wei Bai,Chengdong Xiong
Control of cellular responses is crucial for the use of electrospun membranes in biomedical applications, including tissue engineering or biomedical devices. However, it is still unclear whether adhesion and proliferation of fibroblasts is stimulated or inhibited on polyethylene glycol (PEG)-modified electrospun membranes. In this study, poly( L -lactide-co-glycolide) (PLLGA)–PEG copolymer and pure PEG were blended with PLLGA, and then electrospun onto nonwoven membranes. The effects of blending of PLLGA–PEG or pure PEG on the adsorption of proteins, and further on the adhesion and proliferation of L929 fibroblasts on the electrospun membranes were investigated. Addition of PLLGA–PEG or PEG significantly improved the hydrophilicity of the electrospun membranes. Pure PEG had no obvious effects on the growth of L929 fibroblasts; in contrast, PLLGA–PEG significantly inhibited the adsorption of proteins and the proliferations of the cells on the electrospun membranes. In response to diminished protein adsorption, mRNA expression of genes related to cell adhesion and migration was up-regulated. The limited effects of pure PEG were probably caused by its preferential dissolution, whereas membrane-confined PLLGA–PEG displayed excellent performance on the inhibition of protein adsorption and cell proliferation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:2897–2904, 2012.
研究领域:
生物医学工程
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组织工程
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DOI
PubMed
高影响因子
Materials Research Express
·
2022
·Vol. 9
·期 9
·095101
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IF 2.025
Haibo Chen,Xingli He,Xiaolong Yang,Peng Li,Weibing Wu
Conjugated polymer (CPs) has good near infrared (NIR) absorption and high photothermal conversion effect, which is one of the most promising photothermal therapy (PTT) drugs for cancer therapy. Through the combination of hydrophobic conjugate component of aniline trimer (AT) and hydrophilic component of aldehyde polyethylene glycol (PEG-CHO), linear polymer (PEG-AT-PEG) is synthesized via Schiff base reaction. Self-assembly nanoparticles simply precipitated out by dropping the tetrahydrofuran solution of PEG-AT-PEG into deionized water under ultrasonic dispersion. Owing to the rigid hydrophobic conjugated structure in the middle and the hydrophilic long chain structure at both ends, the long chain material can undergo hydrophobic association and π–π stacking in deionized water to form spherical organic nanoparticle. Such self-assembly nanoparticles have strong NIR absorption at 700 ∼ 800 nm, and can significantly increase the temperature in a short time upon continuous 808 nm laser irradiation, showing good photothermal conversion efficiency. The nanoparticles of PEG-AT-PEG show good biocompatibility to Hela cells at low concentration, when exposed to laser light, the destruction of Hela tumor cells was very distinct in the concentration range of 200 to 800 ug ml−1. Synthetic strategy based on CPs will be possible to develop more effective nanomaterials for treating cancer or tumors.
研究领域:
材料科学
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纳米技术
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癌症研究
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药物化学
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产品关联
DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 25
·期 17
·12458-12468
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IF 3.3
Chufen Yang,Delin Wang,Wenyao Liu,Zexiong Yang,Teng He,Fang Chen,Wenjing Lin
Aiming at achieving the concurrent performances of high loading, well controlled release and active targeted delivery, folate (FA) modified dual pH/reduction-responsive mixed polymeric micelles were rationally assembled using FA-PEG-PDEAEMA and PEG-SS-PCL by dissipative particle dynamics (DPD) simulations. The optimized polymers PEG112-PDEAEMA40, FA-PEG112-PDEAEMA40, and PEG112-SS-PCL70 were synthesized and characterized using 1H NMR, FT-IR and GPC, and their mixed micelles were applied for doxorubicin (DOX) delivery. The drug loading capacity (LC) and encapsulation efficiency (EE) values of the MIX1 (FA-PEG112-PDEAEMA40/PEG112-SS-PCL70) at a DOX/polymer feeding ratio of 15 mg/30 mg were 20.22% and 50.69%, which were higher than those of single polymer micelles and MIX2 (PEG112-PDEAEMA40/PEG112-SS-PCL70). Particle size distributions, mesoscopic morphologies, DPD simulations and in vitro drug release profiles all confirmed the well-controlled release performance of the DOX-loaded micelles formed by MIX1: slow DOX release with a cumulative release of 20.46% in the neutral environment and accelerated release with a cumulative release of 74.20% at pH 5.0 + 10 mM DTT within 120 h, which were similar to those of MIX2. Cytotoxicity assay found that both MIX1 and MIX2 blank micelles were biocompatible, and a superior inhibitory effect of the FA-modified DOX-loaded micelles MIX1 on HepG2 cells was found compared to that of free DOX and non-FA-modified DOX-loaded micelles MIX2. All of these confirmed the superiority of MIX1 micelles with high loading capacity, well controlled release, and enhanced inhibitory effects on HepG2 cells, which might be a prospective candidate for anticancer drug delivery.
研究领域:
药学
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纳米技术
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癌症研究
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产品关联
DOI
PubMed
高影响因子
Materials Advances
·
2022
·Vol. 3
·期 5
·2417-2424
·
IF 0
Yufang Wang,Jianzhu Wang,Mingshuang Sun,Jiyan Zhang,Yanping Bi
Escaping from the immune system to achieve a long circulation time is an essential prerequisite for targeting liposomes. Monomethoxy-poly(ethylene glycol)–distearoylphosphatidylethanolamine (mPEG–DSPE) is the most commonly used stealth polymer for long circulating liposomes. With its monomethoxy end moving freely on the liposomal surface, the linear PEG chains of mPEG–DSPE construct a hydration layer to repel complement proteins and thus evade immune clearance. We describe in this work a new kind of PEG derivative whose two ends were grafted with cholesterol (cholesterol–PEG–cholesterol, CPC). According to the in vitro and in vivo results, the presence of CPC in liposomes was shown to reduce the uptake by phagocyte cells while extending the circulation time. The stealth performance of CPC liposomes is closely related to the molecular weight of PEG moieties. CPC6k performed better than CPC2k, CPC4k and the commonly used mPEG2k–DSPE. CPC on the liposomal surface would not let the free end of PEG act as the immune recognition site, meanwhile, the PEG chain of CPC tends to adopt a twisted ring-like conformation rather than a linear conformation, which is considered to be associated with the stealth effect. This finding adds to the range of stealth liposomes and may be valuable for developing new drug carriers.
研究领域:
药剂学
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生物医学工程
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产品关联
DOI
POLYMER DEGRADATION AND STABILITY
·
2019
·
IF 3.78
Piotr Rychter,Kamila Lewicka,Małgorzata Pastusiak,Marian Domański,Piotr Dobrzyński
Nowadays, researchers in the field of agrochemicals are trying to find effective methods to limit the excessive usage of pesticides and protect the environment. One of the most popular and promising techniques is the immobilization of pesticides using environment-friendly polymers. In the conducted research, it was demonstrated that the family of lactide/glycolide copolymers are unsuitable for this purpose due to very slow degradation in the soil. We examined influence of relatively small content of PEG blocks in newly synthesized poly( l -lactide-co-glycolide)-poly(ethylene glycol)–poly( l -lactide-co-glycolide) (PLGA–PEG–PLGA) on the degradation in soil, in activated sludge and usefulness of this terpolymers as a biodegraded carrier for the controlled release of two commonly used herbicides (Metazachlor and Pendimethalin). Presence of even small amount of PEG block in the terpolymer chain increases slightly the hydrophilicity of the polymeric chain, thereby increasing its susceptibility to hydrolytic and unexpectedly strongly to enzymatic degradation in soil environment. The amorphism of used as a carriers PLGA-PEG-PLGA terpolymers and the lack of the phenomenon of increasing the degree of crystallinity during degradation allow to achieve uniform progress of degradation, and thus is obtained a relatively constantly release of tested herbicides. In this way, it is possible to determine the optimal profile of degradation of polymer in soil and proper rate of diffusion and leaching of active agents which allows the release of herbicides in optimal and effective doses within 3–4 months of its application.
研究领域:
农业科学
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高分子化学
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环境科学
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DOI
高影响因子
Polymers
·
2025
·Vol. 17
·期 3
·422
·
IF 4.9
Qiannian Wang,Yuan Sang,Jiang Yang,Hailing Liu
Polymer corrosion inhibitors are reported to form dense films on carbon steel surfaces, and their thermostability enables survival in harsh downhole environments. In this paper, PEG-OTs was synthesized by mechanochemistry using ball mill by grafting tosyl on PEG. Using this solvent-free green chemistry, non-toxic PEG and PEG-OTs with various molecular weights (600, 2000, and 10,000 g/mol) were prepared and used as corrosion inhibitors. The corrosion inhibition performance of 5 × 10−3mol/L inhibitors on Q235 carbon steel in 0.5 M HCl solution was investigated using static weight-loss, electrochemical impedance spectroscopy, polarization curves, SEM, and contact angle measurements. The results show that, after modification, PEG-OTs has an elevated corrosion inhibition effect compared to PEG. A maximum of 90% corrosion inhibition efficiency was achieved using static weight-loss. The morphology study shows that a dense film formed to protect carbon steel. Thanks to their polymeric structure, a higher molecular weight leads to better corrosion inhibition.
研究领域:
材料科学
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腐蚀与防护
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绿色化学
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产品关联
DOI
PubMed
高影响因子
INTEGRATED FERROELECTRICS
·
2015
·
IF 0.357
Yue-Zhong Wang,Bing Yu,Xiao-Dan Xu,Hua Yuan,Hai-lin Cong,Xiao-Hui Gao
In this paper, we reported an effective microfluidic-based method for fast continuous synthesis of monodisperse PEG/SiO2 hybrid microspheres. The T-junction microfluidic chip equipped with rounded channels and the online photopolymerization system were applied for the microspheres microfabrication. The size of the microspheres was well controlled by the rounded channels and flow rate ratios of immiscrible fluids. We can obtain porous PEG microspheres when the hybrid microspheres were corroded by hydrofluoric acid. What's more, the interpenetrating polymer network structures of SiO2 microspheres were obtained after the hybrid microspheres treated via a calcination process.
研究领域:
材料科学
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化学工程
·
纳米技术
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产品关联
DOI
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2017
·
IF 0
Yang Ou,Zhao-hui Tang,Lu Sun,Hai-yang Yu,Jia Li,Mei-hui Zhao,Hui Xu
Combretastatin A4 (CA4) possesses varying ability to cause vascular disruption in tumors, while the short half-life, low water solubility and deactivation of many CA4 analogs during storage limited its antitumor efficacy and drug stability. A novel macromolecular conjugate of CA4 (CA4-PL) was synthesized by covalent bonding of CA4 onto poly( L -glutamic acid)-graft-polyethylene glycol (PLG-g-PEG) via Yamaguchi reaction. The obtained CA4-PL was characterized by 1 H NMR, GPC, and UV methods, and the properties of the nanoparticles composed of CA4-PL, including critical aggregation concentration, size and size distribution, and morphology, were investigated. CA4-PL can self-assemble to form micelle-like nanoparticles of 80~120 nm in diameter, which may have potential to improve the blood circulation period as well as the targetability of CA4, and find applications to treat various tumors when combined with traditional chemotherapy or radio therapy.
研究领域:
药剂学
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肿瘤学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
CHEMBIOCHEM
·
2024
·
IF 2.6
Xiang Zhu,Weizhe Luo,Donghui Zhang,Runhui Liu
With the increasing use of polyethylene glycol (PEG) based proteins and drug delivery systems, anti-PEG antibodies have commonly been detected among the population, causing the accelerated blood clearance and hypersensitivity reactions, poses potential risks to the clinical efficacy and safety of PEGylated drugs. Therefore, vigilant monitoring of anti-PEG antibodies is crucial for both research and clinical guidance regarding PEGylated drugs. The enzyme-linked immunosorbent assay (ELISA) is a common method for detecting anti-PEG antibodies. However, diverse coating methods, blocking solutions and washing solutions have been employed across different studies, and unsuitable use of Tween 20 as the surfactant even caused biased results. In this study, we established the optimal substrate coating conditions, and investigated the influence of various surfactants and blocking solutions on the detection accuracy. The findings revealed that incorporating 1% bovine serum albumin into the serum dilution in the absence of surfactants will result the credible outcomes of anti-PEG antibody detection.
研究领域:
药学
·
免疫学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）