COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Wei Zhuang,Linjiao He,Jiahua Zhu,Jianwei Zheng,Xiaojing Liu,Yihui Dong,Jinglan Wu,Jingwei Zhou,Yong Chen,Hanjie Ying
The use of graphene oxide (GO) nanosheets for functional enzyme support has attracted intensive interest owing to their unique planar structure and intriguing physical and chemical properties. However, the detailed effects of the interface properties of GO and its functionalized derivatives on active biomolecules are not well understood. We immobilize nuclease P 1 , a common industrial nucleic acid production enzyme, on pristine and amino poly(ethylene glycol) (PEG-NH 2 ) modified GO nanosheets with interface property heterogeneity using two approaches, physical adsorption and chemical crosslinking. It is demonstrated that nuclease P 1 could be stable immobilized on the surface of pristine GO by physical adsorption and on the edge of modified GO nanosheets by chemical crosslinking. The resultant loading capacity of nuclease P 1 on pristine GO is as high as 6.45 mg/mg as a consequence of strong electrostatic and hydrophobic interactions between the enzyme and carrier. However, it is determined that the acid resistance, thermal stability, reusability and degradation efficiency of the immobilized enzyme on PEG-NH 2 -modified GO are obviously improved compared to those of the enzyme immobilized on pristine GO. The enhanced catalytic behavior demonstrates that GO and its derivatives have great potential in efficient biocatalytic systems.
研究领域:
生物化学
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纳米生物学
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酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2023
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IF 8
Yongai Xiong,Tingting Chen,Lei Chen,Rongshan Cai
Introduction Radiotherapy and chemotherapy are the fundamental causes of myelosuppression in cancer patients, which usually induce a serious hematopoietic system toxicity, causing the hemocytes and immunity decline of patients. Ziyuglycoside I (ZgI), an active ingredient isolated from traditional Chinese medicine Sanguisorba officinalis L, has been demonstrated to increase the leucocytes and protect hematopoietic stem cells, which is related to its promotion of autophagy in hematopoietic stem cells.Methods In the present study, we formulated the SH-PEG-NH2-coated gold nanoparticles loading ZgI (ZgI-AuNPs) with a enhanced autophagy promotion in hematopoietic stem cells. ZgI-AuNPs were prepared by HAuCl4-sodium citrate reduction method, and the synthesis of ZgI-AuNPs was validated by XRD, FT-IR, DSC, and TEM findings. Furthermore, the drug loading rate and the release of ZgI were evaluated, and the ZgI-AuNPs’ effects on autophagy and immunofluorescence staining for LC3B were tested. Finally, the effect of ZgI-AuNPs on the autophagy and hematopoietic ability of HSCs in vivo was also carried out.Results The prepared ZgI-AuNPs have an irregular cubic crystal structure by TEM observation, and the average particle size was 340 ± 16.5 nm determined by DLS. The XRD, FT-IR and DSC detection showed that the ZgI had been well loaded in AuNPs, and the AuNPs can load the ZgI at a content of 160.63 ± 1.35 μg·mg−1. Meanwhile, the AuNPs can reduce the drug release rate of ZgI. Importantly, the ZgI-AuNPs enhanced autophagy of HSCs both in vitro and in vivo. At the same time, the gold nanoparticles enhance the hematopoietic effect of ZgI on mice HSCs.Conclusion Our research suggests that SH-PEG-NH2-coated gold nanoparticles loading ZgI has potential application in myelosuppression therapy.
研究领域:
纳米技术
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药理学
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血液学
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肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2017
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IF 2.892
Juan Han,Li Wang,Yun Wang,Jian Dong,Xu Tang,Liang Ni,Lei Wang
A geometrically symmetric four – arm dendritic polymer 4-arm-PEG-NH 2 was employed to modify on the surface of Fe 3 O 4 as support for cellulase immobilization. Glutaraldehyde was used as a coupling agent of this magnetic support to covalently immobilize cellulase. The morphology and properties of the synthesized magnetic material were characterized by Fourier transform infrared (FT-IR) spectroscopy, vibrating-sample magnetometer (VSM), thermogravimetric analysis (TGA), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The properties of immobilized enzyme such as the optimum temperature and pH, Michaelis constant, thermal stability, reusability and storage stability were investigated. The loading capacity of cellulase was 132 mg/g. Compared with free cellulase, immobilized cellulase showed wider pH and temperature ranges, higher operational stability and good storage stability. Besides, the results of hydrolysis filter paper and microcrystalline cellulose showed that the catalytic effect of immobilized cellulase on the free cellulase was increased by about 76%.
研究领域:
生物化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2018
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IF 2.932
Xueqiao Xie,Peng Luo,Juan Han,Tong Chen,Yun Wang,Yunfeng Cai,Qian Liu
In the present study, a novel and efficient immobilization for horseradish peroxidase (HRP) had been developed by using 6-arm magnetic composite microsphere (Fe 3 O 4 @PAA-6-arm-PEG-NH 2 ) containing 6-arm polyethylene glycol (6-arm-PEG-NH 2 ) and Fe 3 O 4 . The morphology and chemical properties of Fe 3 O 4 @PAA-6-arm-PEG-NH 2 were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectra (FTIR), X-ray powder diffraction (XRD), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The immobilization conditions and loading capacity of the carrier toward HRP were also investigated. According to the results, the optimum immobilization conditions were as follows: glutaraldehyde concentration of 0.10 mol L −1 , the carrier concentration of 7 mg L −1 , the temperature of 35 °C and immobilization time of 180 min. The loading capacity of the immobilized HRP arrived to 139.82 mg g −1 (RSD = 0.87%). Compared with free HRP, the stability of the immobilized HRP had been enhanced in a wide range of temperature and pH. The activity of the immobilized HRP retained 71.05% of its initial activity after 60 days storage. It was also found that the activity of the immobilized HRP retained 61.06% of its initial activity after eight times of successive reuse. The immobilized HRP could hydrolyze phenol to 94.4% within 10 min. It proved that the immobilized HRP could improve the performance of HRP, which had a good prospect in biological application.
研究领域:
生物化学
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材料科学
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酶工程
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2024
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IF 18.5
Zhicheng Le,Yufeng Shou,Renee R. Li,Ling Liu,Runcheng Tan,Christopher John Charles,Zhijia Liu,Yongming Chen,Andy Tay
Persistent inflammation, characterized by the intense interplay of inflammatory chemokine secretion and immune cell infiltration, is a hallmark of many skin disorders including diabetic wounds and psoriasis with inadequate therapeutic interventions. Monocyte chemotactic protein-1 (MCP-1) is an inflammatory chemokine that plays a key role in recruiting and polarizing monocytes into pro-inflammatory macrophages to establish a vicious cycle that worsens the inflamed tissue microenvironment. Here, the sponge-like microneedles (HPMN) technology is described to alleviate inflammatory skin disorders. Heparin/4-arm PEG-NH 2 network crosslinked onto microneedle surface spatially attracted and sequestered multiple inflammatory chemokines including MCP-1. Enrichment of MCP-1 on microneedles recruits and traps inflammatory monocytes within the porous structure of microneedles. Subsequent removal of microneedles not only depletes inflammatory chemokine, MCP-1, but also its cellular source. As a result, HPMN treatment facilitates 47.1% smaller open wound area in mice and 27.2% shorter wound length in pigs. To demonstrate the versatility of the HPMN technology, it is also shown that combining the method with standard-of-care immunosuppressants reduces 45.1% epidermis thickening and attenuated immune cell influx in a mouse psoriasis model. Overall, the HPMN technology is a novel demonstration of employing inflammatory chemokine and cell extraction to treat a broad range of inflammatory skin disorders.
研究领域:
生物医学工程
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皮肤科
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炎症
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伤口愈合
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药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
Polymers
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2022
·Vol. 14
·期 21
·4694
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IF 4.967
Ran Yu,Chao Xu,Xiaopei Wu,Honglian Dai
We designed and synthesized the polyoxyethylene diamine (H2N-PEG-NH2) and poly(amide-imide)-polyethylene glycol (PAI-PEG) copolymers. The physical and chemical properties, mechanical properties, and in vitro biocompatibility of the materials were characterized. The results showed that the best elongation at break and recovery were obtained when the amount of PEG was 5 wt%. With the increase in PEG content, the degradation rate, hydrophilic property, tensile strength and tensile modulus of the copolymer decreased to a certain extent. The material had the best thermal stability and mechanical properties when 5 wt% PEG was added. Cytocompatibility evaluation showed that the addition of PEG could enhance the cell compatibility of the material and make it potentially suitable for application in bone repair.Keywords:polyoxyethylene diamine (H2N-PEG-NH2);poly(amide-imide) (PAI);biocompatibility
研究领域:
材料科学
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生物医学工程
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生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2018
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IF 5.08
Meng Li,Ying Wang,Huiming Lin,Fengyu Qu
Here, hollow CuS nanocubes about 250–300 nm in size were synthesized based on the Kirkendall effect by using CuO nanocubeas precursor and template. The reactant concentration and reaction time could be adopted to adjust the final composition and hollow structure. The as-synthetic CuS nanocube was assembled by a great deal of nanoparticles (15–20 nm), making abundant porous structure in the shell layer. The localized surface plasmon resonance and the novel porous hollow structure (improve light reflex) further make sure the enhanced Near-infrared (NIR) light absorption as well as photothermal conversion efficiency (30.3%). Moreover, the mechanism of reactive oxygen species (ROS) generation was investigated in detail, revealing that the released Cu + ion and the oxygen are the determined factors. To further improve the monodispersity and biocompatibility, PEG-NH 2 modified nanostructure (CuS@PEG) was prepared and it possessed high loading efficiency to doxorubicin hydrochloride (DOX). Moreover, DOX-CuS@PEG reveals the acid and NIR sensitive-release performance. The synergistic effect of chemotherapy associated with photothermal therapy (PTT) and photodynamic therapy (PDT) display the enhanced specific cytotoxicity to cancer cells.
研究领域:
纳米技术
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癌症治疗
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2023
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IF 9.7
Qiying Lv,Kai Chi,Xiaolei Shi,Miaodeng Liu,Xiaoye Li,Cheng Zhou,Lin Shi,Huiling Fan,Huan Liu,Jia Liu,Yan Zhang,Shuai Wang,Lin Wang,Zheng Wang
Selectively generating active free radical (AFR) in tumor microenvironment (TME) can promote irreversible oxidation of biomolecules and damage tumor cells, resulting in effective tumor inhibition. However, therapeutic efficacy of AFR-based tumor suppression approaches is often limited by insufficient amount of H 2 O 2 or O 2 within TME. To overcome this obstacle, we design a pH/photothermal dual responsive nanosystem ( [email protected] ) for combined photothermal and nanocatalytic therapy in the near-infrared biowindow. Here the Fe single-atom dispersed N, S-doped carbon nanosheets (FeSA) nanozyme is dispersed by phospholipid-polyethylene glycol-amine (DSPE-PEG-NH 2 ), and further loads artesunate (AS) via an amide reaction. Upon 808-nm laser irradiation in TME, the AS is released and further be catalyzed by the FeSA nanozyme to produce cytotoxic C-centered AFRs, and further be accelerated due to the photothermal conversion performance of FeSA (23.35%). The nanocatalytic process of FeSA nanozyme is realized by density functional theory (DFT). The tumor inhibition rates of a CT26 xenograft model is 92% through a photothermal-enhanced nanocatalytic synergistic therapy, and negligible systematic toxicity is observed. This work offers a potential paradigm of multifunctional single atomic catalysts (SACs) for enhancing tumor nanocatalytic therapy. Statement of significance We designed a pH/photothermal dual responsive nanosystem ( [email protected] ) for nanocatalytic therapy: (1) the nanosystem responsively releases AS under 808-nm laser irradiation in TME; (2) FeSA in the nanosystem can act as heme mimetic to convert AS into high cytotoxic C-centered free radicals for nanocatalytic therapy; (3) the photothermal conversion performance of FeSA further enhances the catalytic process to yield abundant AFR. Both in vitro and in vivo results demonstrate that this nanosystem can efficiently inhibit tumor growth through a photothermal-enhanced nanocatalytic synergistic therapy.
研究领域:
癌症治疗
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纳米技术
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光热疗法
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药物递送系统
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）