Materials Science & Engineering C-Materials for Biological Applications
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2013
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IF 2.404
Wanyun Liu,Junchao Wei,Ping Huo,Yunhua Lu,Yiwang Chen,Yen Wei
In this work, PEG–PLLA electrospun fibers were developed as a new controlled release system for macrolide antibiotic drug brefeldin A (BFA). SEM and XRD analyses of the BFA-loaded PEG–PLLA fibers revealed that the average diameter of fibers was below 950 nm with smooth surfaces, and the drug was well incorporated into the fibers in amorphous form. The release profiles of BFA in PBS were measured by HPLC, demonstrating that the controlled release of BFA could be gained for long time. The in vitro antitumor activity against human liver carcinoma HepG2 cells of the fibers containing 3%, 6%, 9%, 12% and 15% BFA were examined by MTT method, and the results showed that cell growth inhibition rates at 72 h were 64%, 77%, 80%, 81% and 85%, respectively. These results strongly suggested that the BFA/PEG–PLLA fibers had an effect of controlled release of BFA and were suitable for postoperative chemotherapy of cancers.
研究领域:
药学
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肿瘤学
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材料科学
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PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1081
·
IF 4.2
Jieqiong Ding,Jie Su,Binhua Luo,Liqiong Ding
Lycorine is a kind of natural active ingredient with a strong antitumor effect. In this study, folate ligand-conjugated polyethylene glycol-block-poly(l-lactide) (PEG-PLLA) nanoparticles (FA-PEG-PLLA NPs) were designed to deliver lycorine to enhance its anti-glioma activity. The successful preparation of the FA-PEG-PLLA polymer was confirmed by1H-NMR, FT-IR, XRD, TGA, and DSC. The optimal formulation for LYC@FA-PEG-PLLA NPs was determined by response surface analysis as follows: sodium dodecyl sulfate (SDS) of 1%, carrier material of 0.03 g, organic phase volume of 3 mL, and ultrasonic power of 20%. The LYC@FA-PEG-PLLA NPs exhibited an encapsulation efficiency of 83.58% and a particle size of 49.71 nm, demonstrating good stability. Hemolysis experiments, MTT assays, and cell scratch assays revealed excellent biocompatibility of FA-PEG-PLLA and superior anti-glioma activity of LYC@FA-PEG-PLLA NPs compared to the raw drug. Additionally, cell apoptosis assays, ROS experiments, and western blot analysis demonstrated that LYC@FA-PEG-PLLA NPs contributed to cell apoptosis by inducing ROS generation and increasing the expression of NF-κB inhibitory protein IκBα. These results suggested that LYC@FA-PEG-PLLA NPs hold promise for glioma treatment.
研究领域:
药学
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纳米技术
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肿瘤学
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
Journal of Functional Biomaterials
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2026
·Vol. 17
·期 2
·71
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IF 5.2
Siqi Zhang,Yuan Gao,Danyang Wang,Yongjie Chi,Fang Wu,Lianyan Wang,Hailan Jin
Skin aging could lead to dermal collagen loss and elastic fiber degradation, ultimately manifesting as skin laxity. We aimed to counteract this by using poly-L-lactic acid (PLLA) microsphere (MS)-based fillers to facilitate long-term volume restoration through collagen regeneration. However, conventional MSs exhibit limitations such as broad size distribution and surface irregularities, which are frequently associated with significant adverse reactions. This study employed shirasu porous glass (SPG) membrane emulsification to fabricate uniform and well-shaped polyethylene glycol-block-poly (L-lactic acid) (PEG-PLLA) MSs. A single-factor experiment was employed to optimize the parameters. The optimal preparation conditions for PEG-PLLA MSs were as follows: PEG-PLLA concentration of 40 mg/mL, polyvinyl alcohol (PVA) concentration of 0.5%, and magnetic stirring speed of 200 rpm. Under the optimal conditions, the average particle size of PEG-PLLA MSs was 58.982 μm, and the span value (SPAN) was 1.367. In addition, a cytotoxicity assay was performed, and the results revealed no significant toxicity of the MSs toward L929 mouse fibroblasts at concentrations below 500 μg/mL. Furthermore, PEG-PLLA MSs significantly enhanced the production of key extracellular matrix (ECM) components—type I collagen (Col-I), type III collagen (Col-III), and hyaluronic acid (HA)—while simultaneously alleviating cellular oxidative stress responses. This work offers a reliable and reproducible fabrication strategy for developing biocompatible MS fillers with controllable particle sizes.
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高影响因子
ACS Applied Polymer Materials
·
2021
·
IF 4.089
Wenyuan Xie,Chenguang Jiang,Xing Yu,Xinyu Shi,Shubin Wang,Yu Sun,Ming Yin,Defeng Wu
Biodegradable polymersomes that can potentially be employed in drug delivery systems were fabricated by blending poly(l-lactide)-b-poly(ethylene glycol)-b-poly(l-lactide) (PLLA-PEG-PLLA) and poly(d-lactide)-b-poly(ethylene glycol)-b-poly(d-lactide) (PDLA-PEG-PDLA) block copolymers in a weight ratio of 1:1 in an aqueous solution. A series of amphiphilic PLLA-PEG-PLLA and PDLA-PEG-PDLA triblock copolymers were synthesized with different hydrophobic chain lengths through ring-opening polymerization. The self-assembly of pure PLLA-PEG-PLLA and an equimolar mixture of enantiomers of PLLA-PEG-PLLA and PDLA-PEG-PDLA was investigated by transmission electron microscopy, X-ray diffraction, and dynamic light scattering. Various nanostructures including spherical micelles, worm-like micelles, and vesicles were obtained by controlling the hydrophobic chain length and the incubation temperature. In the pure PLLA-PEG-PLLA systems, the hydrophobic–hydrophilic balance dominated the self-assembled morphologies. In equimolar enantiomer mixtures, self-assembled morphologies were greatly affected by the formation of the PLLA-PDLA stereocomplex (SC). There was an inverse correlation between the SC crystallinity and the curvature of the self-assembled structures─the higher the SC content, the smaller the curvature. This rule was not applicable when the SC content was too high (≳50%), which produced spherical micelles as the main nanostructures. Since the SC-induced self-assembly could be controlled by changing the incubation temperature, the polymersomes can potentially be used in many applications, such as nanoreactors and nanovehicles.
研究领域:
材料科学
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药物递送系统
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高分子化学
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DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2020
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IF 2.69
Jianping Mao,Pengfei Wei,Zuoying Yuan,Wei Jing,Jingjing Cao,Guangping Li,Jianxun Guo,Honggang Wang,Dafu Chen,Qing Cai
There are intensive needs for scaffolds with new designs to meet the diverse requirements of bone repairing. Biodegradable microspheres are highlighted as injectable micro-scaffolds thanks to their advantages in filling irregular defects via a minimally invasive surgery. In this study, microspheres with surface micropores were made via the W1/O/W2 double emulsion method using amphiphilic triblock copolymers (PLLA-PEG-PLLA) composed of poly(L-lactide) (PLLA) and poly(ethylene glycol) (PEG) segments. When the PEG fraction was controlled as 10 wt.%, the microspheres demonstrated higher cell affinity than the smooth-surfaced PLLA microspheres. After being further functionalized with polydopamine coating and apatite deposition, the PLLA-PEG-PLLA microspheres could up-regulate the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSCs) significantly. Before subcutaneous implantation, bone morphogenetic protein-2 (BMP-2) was adsorbed onto the biomineralized microspheres by taking advantages of the strong affinity of apatite to BMP-2. The resulted microspheres induced ectopic osteogenesis efficiently without causing biocompatibility problems. In summary, this study provided a simple strategy to prepare functionalized microspheres with osteoconductivity and osteoinductivity, which showed great potential in promoting bone regeneration as injectable micro-scaffolds.
研究领域:
生物医学工程
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组织工程
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材料科学
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再生医学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Biao-Qi Chen,Ranjith Kumar Kankala,Yang Zhang,Shu-Ting Xiang,Han-Xiao Tang,Qi Wang,Da-Yun Yang,Shi-Bin Wang,Yu Shrike Zhang,Gang Liu,Ai-Zheng Chen
In an attempt to attain synergistic therapeutic benefits and address various intrinsic limitations of the highly efficient black phosphorus quantum dots (BPQDs), we fabricated poly( L -lactide)-poly(ethylene glycol)-poly( L -lactide) triblock copolymer (PLLA-PEG-PLLA)-based nanocomposites co-loaded with BPQDs and gambogic acid (GA) using the supercritical carbon dioxide (SC-CO 2 ) technology to achieve photoacoustic (PA) imaging-guided synergistic chemo-photothermal therapy. On the one hand, BPQDs displayed near-infrared (NIR)-induced hyperthermia through the high photothermal conversion efficiency. On the other hand, the NIR-responsive release of GA facilitated early apoptosis through specific binding to stress-induced overexpression of heat shock protein (HSP)-90 for combating thermoresistant tumor cells. GA significantly promoted the photothermal therapy (PTT) efficiency by enhancing both early and late apoptosis of BPQDs. Moreover, the encapsulation of BPQDs in the polymer significantly improved their chemical as well as photothermal stabilities. Our findings suggested that these nanocomposites fabricated using the eco-friendly supercritical fluid (SCF) technology provided good protection to the biodegradable BPQDs, offering a great potential towards cancer ablation through augmented synergistic effects.
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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材料科学
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药学
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DOI
高影响因子
危险品化学品经营许可证（带存储）