Analytical Methods
·
2023
·Vol. 16
·期 1
·51-61
·
IF 3.1
Jing Qi,Qianyi Qi,Zhou Zhou,Yixuan Wu,Aiting Cai,Jinran Wu,Bairong Chen,Qingxiang Wang,Lin Chen,Feng Wang
The detection of epidermal growth factor receptor (EGFR) mutation L858R in circulating tumor DNA (ctDNA) is beneficial for the clinical diagnosis and personalized therapy of non-small cell lung cancer (NSCLC). Herein, for the first time, the combination of the primer exchange reaction (PER) and clustered regularly interspaced short palindromic repeats (CRISPR) and its associated nucleases (Cas) 14a was used in electrochemical biosensor construction for the detection of ctDNA EGFR L858R. EGFR L858R, as the target, induced the isothermal amplification of the PER reaction, and then the CRISPR/Cas14a system was activated; subsequently, the substrate ssDNA-MB was cleaved and the electron on the surface of the gold electrode transferred, resulting in the fluctuation of the electrochemical redox signal on the electrode surface, whereas the electrochemical signal will be stable when EGFR L858R is absent. Therefore, the concentration of EGFR L858R can be quantified by electrochemical signal analysis. The low detection limit is 0.34 fM and the dynamic detection range is from 1 fM to 1 μM in this work. The PER-CRISPR/Cas14a electrochemical biosensor greatly improved the analytical sensitivity. In addition, this platform also exhibited excellent specificity, reproducibility, stability and good recovery. This study provides an efficient and novel strategy for the detection of ctDNA EGFR L858R, which has great potential for application in the diagnosis and treatment of NSCLC.
研究领域:
分子生物学
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临床诊断
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癌症研究
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
TETRAHEDRON
·
2017
·
IF 2.651
Zhenyu Tian,Kun Meng,Yanhong Shi,Naixia Zhang,Xuehui Liu,Hongwei Jin,Yongmin Zhang,Lihe Zhang,Demin Zhou,Sulong Xiao
A newly synthesized hexavalent oleanolic acid/per- O -methylated α -cyclodextrin conjugate shows significant differences in NMR spectra with previously synthesized analogs. Characterization of the product by 1 H NMR, 13 C NMR, COSY, HSQC , HMBC , TOCSY , NOE, and ROESY experiments were performed. Detailed investigations revealed that the compound has two conformations in solution and the ratio of them was 1:1. Further variable-temperature NMR study revealed that the two conformations were stable at temperature range of 273–323 K in CDCl 3 solution.
研究领域:
有机化学
·
药物化学
✓ 已验证
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Ai Ting Cai,Qing Ping Fu,Ming Zhong Sun,Hong Mei Chen,Yun Gang Wang,Hui Xiang Jv
The detection of BRAF mutations in circulating tumor DNA is beneficial for early diagnosis and personalized therapeutic approaches of melanoma. However, the sensitive and reliable trace detection of BRAF mutations remains a major challenge. Therefore, in this study, the combination of two isothermal signal amplification techniques (primer exchange reaction (PER) and hybridization chain reaction (HCR)) was proposed to construct an electrochemical biosensor for the efficient and sensitive detection of BRAF mutations. The presence of target triggered PER and HCR sequentially to achieve dual-cascade amplification of positive electrochemical signal feedback. Compared to the detection limit (1.27 pM or 0.23 pM) of single-signal amplification technology (PER or HCR), the detection limit of dual-signal amplification techniques was significantly lower (0.34 fM). In addition, the dual-signal amplification strategy exhibited other excellent sensing properties, such as a wide linear detection range (0.01 pM to 1 nM), excellent specificity, satisfactory reproducibility and stability, and the ability to achieve low-level target detection in diluted serum samples. Based on all of these features, the proposed strategy indicate broad application prospects for detecting low-abundance nucleic acid targets in clinical samples.
研究领域:
生物传感器
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分子诊断
·
生物化学
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癌症研究
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分析化学
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遗传学
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电化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Jingni Fu,Luning Zhang
Relying on the nanometer-thick water core and large surface area-to-volume ratio (∼2 × 108 m–1) of common black film (CBF), we are able to use a pH-sensitive dye (carboxy-seminaphthorhodafluor-1, SNARF-1) to detect ammonia and acetic acid gas adsorption into the CBF, with the limit of detection reaching 0.8 ppm for NH3 gas and 3 ppb for CH3COOH gas in the air. Data analysis reveals that fluorescence signal change is linearly proportional to the gas concentration up to 15 ppm and 65 ppb for NH3 and CH3COOH, respectively.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Qi Hua,Xuepeng Chi,Wei Zhang,Hongyu Song,Ying Wang,Zhenguo Liu,Hongfang Wang,Baohua Xu
Monocrotaline (MCT) is a toxic pyrrolizidine alkaloid found in plants of the Crotalaria genus. As primary pollinators of Crotalaria plants, honeybees come into contact with this harmful substance. However, limited research has been conducted on the effects of MCT on Apis mellifera , particularly the risks of long-term exposure to sublethal concentrations. Through evaluating the proboscis extension reflex (PER) ability, analyzing the honeybee brain transcriptome, and analyzing the honeybee hemolymph metabolome, we discovered that sublethal concentrations of MCT impair the olfactory and memory capabilities of honeybees by affecting tryptophan (Trp) metabolism. Furthermore, MCT upregulates the expression of the corazonin receptor ( CrzR ) gene in the honeybee brain, which elevates reactive oxygen species (ROS) levels in the brain while reducing glucose levels in the hemolymph, consequently shortening the honeybees' lifespan. Our findings regarding the multifaceted impact of MCT on honeybees lay the foundation for exploring its toxicological pathways and management in honeybee populations.
研究领域:
昆虫学
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毒理学
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分子生物学
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DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2014
·Vol. 68
·期 8
·1132-1136
·
IF 1.193
Tao Hong-Wen,Wang Xia,Yi Ping-Gui,Deng Zhi-Hong,Yu Xian-Yong,Li Xiao-Fang
A class of stereo-isomerically-pure alkanediol monoglycosides, 2-hydroxyethyl per- O -acetyl pyranosides ( IIIa-IIIf ), was conveniently prepared by a one-pot reaction of per- O -acetylated pyranoses ( Ia-If ) and 2-( tert -butyldimethylsilyloxy)ethanol ( II ) with catalysis by BF 3 ·OEt 2 . The α -( IIIa ) or β -linked glycosides ( IIIb-IIIf ) with 1,2- trans -configuration were obtained from glycosyl donors with participation of the neighbouring 2- O -acetyl group. BF 3 ·OEt 2 , along with hydrogen fluoride released from BF 3 ·OEt 2 under the experimental conditions used, facilitates the subsequent de-protection of siloxane to successfully afford 2-hydroxyethyl per- O -acetyl-pyranosides.
研究领域:
有机化学
·
糖化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Chihong Ma,Qin Zhou,Jinjin Shi,Hua Gao,Di Huang,Huimin Xue,Han Wang,Zhenzhong Zhang,Sen Yang,Junli Zhang,Kaixiang Zhang
The clustered regularly interspaced short palindromic repeats (CRISPR) system provides a new molecular diagnostic tool for construction of biosensor platforms due to its high programmability and target specificity. Herein, we developed a CRISPR-empowered electrochemical biosensor by combining the advantages of CRISPR/Cas13a and primer exchange reaction (PER), named PER-E-CRISPR, for target amplification-free and sensitive detection of miR-21. Dual-signal amplification procedures involve the binding of target miR-21 induced by CRISPR-based amplification, along with the hybridization of multiple short single-stranded DNA strands with PER concatemers. When target miR-21 is present, CRISPR/Cas13a specifically recognizes the target miRNA , triggering the trans -cleavage activity of CRISPR/Cas13a. Then Cas13a/crRNA/miRNA cleaved the predesigned ribonucleotide site in hairpin 1 (HP1) and released trigger to open hairpin 2 (HP2) modified on the electrode surface. Then PER bridge sequence contained in HP2 is exposed and hybridized with PER concatemers, following multiple short single-stranded DNA tagged with methylene blue (ssDNA-MB) bond with the PER concatemers. Under optimized conditions, PER-E-CRISPR assay for detecting miR-21 exhibits linearity in dynamic range from 10 −13 to 10 −7 M, and we obtained a limit of detection (LOD) of 30.2 fM. The established PER-E-CRISPR biosensor shows perfect practical performance in actual plasma, which may have great promising prospects for miRNA detection in the field of molecular diagnosis.
研究领域:
分子诊断
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生物技术
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电化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Kun Zhang,Han Yan,Xiaoduo Chen,Zusen Chen,Xiaonan Feng,Ya Hu,Zheqian Chen,Xiaoyuan Guo,Chuanzi Gao,Yanzhou Bao,Jizhou Wu,Bintian Zhang
Cationic per- and polyfluoroalkyl substances (PFAS) are emerging environmental contaminants of growing concern, yet rapid and reliable sensing strategies remain scarce due to limited molecular selectivity and poor resistance to false-positive signals. Herein, we report a portable electrochemical sensor for perfluorooctane sulfonamide quaternary ammonium (PFOSAmS) based on a molecularly imprinted poly(4-hydroxybenzoic acid) membrane integrated onto a disposable screen-printed electrode. Molecular electrostatic potential analysis reveals strong anionic sites within the imprinted polymer that favor electrostatic attraction to the cationic headgroup of PFOSAmS, while the aromatic backbone provides hydrophobic domains complementary to the perfluorinated chain. The formation of imprinted cavities effectively enhances the selectivity for PFOSAmS over structurally related PFAS. Specific binding of PFOSAmS generates a dual electrochemical response, characterized by a decrease in probe current and a positive shift in redox potential, enabling intrinsic discrimination of false-positive signals. Nonspecific adsorption only reduces interfacial electron transfer without affecting the redox potential, whereas binding within the imprinted cavities disturbs the local electrostatic and dielectric environment, leading to a characteristic potential shift. Under optimized conditions, the sensor exhibits a detection range from 50 pM to 5 µM with a detection limit of 7 pM (5.1 ng/L). Selectivity evaluation against 20 potential interferents, including seven representative PFAS, confirms negligible influence. Practical detection of PFOSAmS in tap, bottled, and river water samples further demonstrates the robustness of the sensing platform. This work establishes a rapid, low-cost, and false-positive-resilient analytical tool for sensitive and accurate detection of cationic PFAS.
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Wang Shixun,Wang Shengnan,Wei Zhiquan,Wang Yiqiao,Zhang Dechao,Chen Ze,Zhi Chunyi
Zinc-ion batteries have demonstrated promising potential for future energy storage, whereas drawbacks, including dendrite growth, hydrogen evolution reaction, and localized deposition, heavily hinder their development for practical applications. Herein, unlike elaborated structural design and electrolyte excogitation, we introduce an effective parts-per-million (ppm)-scale electrolyte additive, phosphonoglycolic acid (PPGA), to overcome the intrinsic issues of zinc negative electrode in mild acidic aqueous electrolytes. Profiting from absorbed PPGA on zinc surface and its beneficial interaction with hydrogen bonds of adjacent water molecules, stable symmetric stripping/plating of zinc in aqueous ZnSO 4 electrolyte at around 25 o C was achieved, procuring 362 and 350 days of operation at 1 mA cm -2 , 1 mAh cm -2 and 10 mA cm -2 , 1 mAh cm -2 , respectively. As a proof-of-concept, an Ah-level Zn||Zn 0.25 V 2 O 5 ·nH 2 O pouch cell examined the validity of PPGA and sustained 250 cycles at 0.2 A g -1 and around 25 o C without capacity loss. The Zn||Br 2 redox flow battery demonstrated an operation of over 800 h at 40 mA cm -2 , 20 mAh cm -2 with an average coulombic efficiency of 98%, which is attributed to restrained dendrite growth and side effects. This work is believed to open up new ways forward for knowledge of electrolyte additive engineering.
研究领域:
能源存储
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Dengdiao Yao,Jian Shao,Dantong Jia,Weiling Sun
Hexafluoropropylene oxide dimer acid (HFPO-DA) and perfluoroethylcyclohexane sulfonate (PFECHS) are increasingly used as alternatives for perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS). However, their immunotoxicity and underlying molecular mechanisms remain poorly understood. Here, to assess immunotoxic effects, zebrafish embryos were exposed to environmentally relevant concentrations of PFOA, PFOS, HFPO-DA, and PFECHS for four days. Results revealed that all four per- and polyfluoroalkyl substances (PFAS) resulted in decreased heart rate and spontaneous movement, and induced oxidative stress in zebrafish larvae. Notably, HFPO-DA exhibited more severe oxidative stress than PFOA. Immune dysfunction was observed, characterized by elevated cytokine, complement factor, nitric oxide, and neutrophil content, along with a significant decrease in lysozyme content. Transcriptomic analysis revealed the activation of Toll-like receptor (TLR)/NOD-like receptor (NLR)/RIG-I-like receptor (RLR) and associated downstream genes, indicating their pivotal role in PFAS-induced immunomodulation. Molecular docking simulations demonstrated stable interactions between PFAS and key receptors (TLR2, NOD2 and RIG-I). Overall, HFPO-DA and PFECHS exhibited immunotoxic effects in zebrafish larvae similar to legacy PFAS, providing important information for understanding the toxic mode of action of these emerging alternatives.
研究领域:
环境毒理学
·
免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2012
·
IF 2.332
Liqiang Wu,Zhikui Yin
A simple and clean synthesis of per- O -acetylation carbohydrate derivatives has been accomplished by treatment of sugars with a stoichiometric quantity of acetic anhydride under solvent-free conditions in the presence of sulfonic acid functionalized nano γ-Al 2 O 3 as an efficient and environmentally benign catalyst.
研究领域:
有机化学
·
催化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
eLife
·
2025
·
IF 0
Gaohang Wang,Wei Qi,Rui Huang,Liming Wang
Sweet taste perception, a critical aspect of the initiation of feeding behavior, is primarily regulated by an animal’s internal metabolic state. However, non-metabolic factors, such as motivational and emotional states, can also influence peripheral sensory processing and hence feeding behavior. While mating experience is known to induce motivational and emotional changes, its broader impact on other innate behaviors, such as feeding, remains largely uncharacterized. In this study, we demonstrated that the mating failure of male fruit flies suppressed sweet taste perception via dopamine signaling in specific neural circuitry. Upon repetitive failure in courtship, male flies exhibited a sustained yet reversible decline of sweet taste perception, as measured by the proboscis extension reflex (PER) towards sweet tastants as well as the neuronal activity of sweet-sensing Gr5a+ neurons in the proboscis. Mechanistically, we identified a small group of dopaminergic neurons projecting to the subesophageal zone (SEZ) and innervating with Gr5a+ neurons as the key modulator. Repetitive sexual failure decreased the activity of these dopaminergic neurons and in turn, suppressed Gr5a+ neurons via Dop1R1 and Dop2R receptors. Our findings revealed a critical role for dopaminergic signaling in integrating reproductive experience with appetitive sensory processing, providing new insights into the complex interactions between different innate behaviors and the role of brain’s reward systems in regulating internal motivational and emotional states.
研究领域:
神经科学
·
行为生物学
·
感觉生物学
·
分子神经科学
·
神经行为学
✓ 已验证
产品关联
DOI
PubMed
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