JOURNAL OF PLANT GROWTH REGULATION
·
2019
·Vol. 39
·期 2
·631-640
·
IF 2.179
Jia Chunhua,Yu Xiaojing,Zhang Min,Liu Zhiguang,Zou Peng,Ma Jun,Xu Yachun
The growth and development of cold-season plants are susceptible to high temperature. Melatonin is a plant growth regulator with potential to improve plant tolerance to biological and abiotic stresses. In our study, cherry radish ( Raphanus sativus L. var. radculus pers ) was cultured at a high temperature (35 °C/30 °C day/night) and different concentrations (0, 11.6, 17.4, 29.0, 34.8 and 67.0 mg L −1 ) of melatonin were applied to these high-temperature stressed plants to its effects on biomass, quality, antioxidant enzyme activity, chlorophyll and endogenous hormone contents. The plants were grown under normal temperature (25 °C/20 °C) as control and high-temperature condition as HT-stress treatment. The results revealed that under high temperature with 29.0 mg L −1 melatonin treatment, cherry radish biomass was significantly increased by 12.9%, and the soluble protein and soluble solid were increased by 18.7 and 9.2%, respectively. The activity of antioxidant enzyme, ascorbate peroxidase and peroxidase were increased by 43.7 and 45.5%, respectively. The chlorophyll a and carotenoid contents were increased by 7.4 and 20.0% compared with the control at 27 days. The auxin and abscisic acid contents were significantly increased by 28.5 and 6.7% compared with HT at 9 days. Thus, application of optimal rate of melatonin had a positive effect on cherry radish growth under high-temperature stress.
研究领域:
植物学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiaojiao Hou,Huxuan Gong,Zan Gong,Xiao Tan,Xiangxiang Qin,Jing Nie,Hua Zhu,Shian Zhong
We reported here an interesting source of Alpinia zerumbet Polysaccharides (named AZPs) from the residues after extracting essential oil by steam distillation from Alpinia zerumbet fructus. After a series of purifications, a homogeneous polysaccharide (AZP-2) of molecular weight 1.25 × 10 5 Da was obtained. Structure, anti-inflammatory activity, and anti-inflammatory mechanism were investigated. AZP-2 was mainly composed of galactose, arabinose, xylopyranose, glucose, and galacturonic acid. The main linkage structure of AZP-2 was determined after integrating the nuclear magnetic resonance (NMR) and methylation analysis, and the structure was comparatively complex. The results indicated that AZP-2 significantly decreased the production of NO and ROS in the inflammatory model established by lipopolysaccharide (LPS) stimulated RAW264.7, particularly at the concentration of 200 μg/mL. Furthermore, AZP-2 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines. Notably, the mechanism of AZP-2 exhibiting inhibitory effects was related to regulating the NF-κB signaling pathway. Overall, AZP-2 could be used as a potential anti-inflammatory agent for further in-depth studies.
研究领域:
药理学
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生物化学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 40
·期 2
·472-479
·
IF 2.741
Guoyan Liu,Linwu Zhuang,Dandan Song,Chunliang Lu,Xin Xu
We developed a simple and meaningful preparative method for the separation and purification of the main phenolic compounds from the leaves of celery ( Apium graveolens L. var. dulce Mill./Pers.) and we established an accurate and specific analytical method for the identification of the main phenolic compounds from celery leaves. The crude extract from celery leaves was prefractioned by polyamide resin to enrich the phenolic compounds. They were then purified further by preparative high-performance liquid chromatography, and seven main phenolic compounds were obtained: including chlorogenic acid, luteolin 7- O -β -d- apiofuranosyl(1→2)-β -d- glucopyranoside, luteolin 7- O -β -d- glucopyranoside, apiin, chrysoeriol 7- O -β -d- apiofuranosyl(1→2)-β -d- glucopyranoside, luteolin 7- O -[β -d- apiofuranosyl(1→2)-(6′′- O -malonyl)]-β -d- glucopyranoside, and apigenin 7- O -[β -d- apiofuranosyl(1→2)-(6′′- O -malonyl)]-β -d- glucopyranoside. Their purities were measured by using high-performance liquid chromatography, and their chemical structures were confirmed using UV spectrophotometry, ultra high performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry, and NMR spectroscopy. Our studies indicate that preparative high-performance liquid chromatography combined with polyamide resin is a simple and meaningful preparative method for the separation and purification of phenolic compounds from the leaves of celery or other plants, and the use of UV spectrophotometry, ultra high performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry, and NMR spectroscopy is an accurate and specific analytical method for the identification of phenolic compounds.
研究领域:
生药学
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分析化学
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植物科学
✓ 已验证
DOI
PubMed
高影响因子
Eco-Environment & Health
·
2025
·
IF 17.6
Lianzhou Shen,Yue Cai,Juan Gao
Nanoscale zero-valent iron loaded on biochar (nZVI@BC) has been proven to be effective in activating persulfate to remediate soil organic pollutants. However, studies on subsequent plant growth and microbial community changes in remediated soil remain limited. In this study, nZVI@BC, nZVI, and nanoscale biochar (nBC) were ball-mill produced and applied as amendments in pot experiments with PAH-contaminated soil to investigate their impacts on soil-crop (radish, Raphanus sativus L.) systems, and the widely distributed phenanthrene (Phe) was selected as model pollutant. The results indicate that nZVI@BC could induce more (75%) Phe accumulation in radish compared to the control treatment, but did not result in significant differences in plant biomass or enzyme activity. In Phe contaminated treatments, the Fe content of radish shoots increased from 86.87 ± 5.61 mg/kg DW without material application to 125.20 ± 11.93 mg/kg DW with nZVI@BC, while no significant differences were observed in roots. nZVI@BC and nBC increased the non-desorbed fraction of PAHs with low bio-availability by 13.6% and 10.2%, respectively, after 45 days compared to the control treatment. Illumina MiSeq sequencing revealed that nZVI@BC did not adversely affect the richness and diversity of soil microbial communities. Instead, it promoted the enrichment of bacteria related to the degradation of organic pollutants, such as Lysobacter and Spingomonas . The findings suggest that nZVI@BC after chemical oxidation remediation might be harmful to subsequent plants and ecosystems but much better than nZVI alone. The amount of nZVI@BC should be accurately calculated before chemical oxidation remediation.
研究领域:
环境科学
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生态学
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农业科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Yu Guo,Yan Li,Zhuang Li,Li Jiang,Xifeng Cao,Wenbin Gao,Jun Wang,Duqiang Luo,Fengli Chen
As an important natural product, the sufficient separation of plant essential oil (EO) is helpful to improve its utilization value. In this work, deep eutectic solvent-homogenate based microwave-assisted hydrodistillation (DES-HMAHD) was developed and applied to isolate EO from the fruits of Litsea cubeba (Lour.) Pers. Different types of DES were investigated in terms of the EO kinetics and composition, among which oxalic acid/choline chloride (OA/ChCl) had obvious advantages. Following, molar ratio of OA and ChCl (1:1), water content (50%), liquid-solid ratio (12.5:1 mL/g), homogenate time (2 min), and microwave power (700 W) were found to be the optimum conditions. Gas chromatography-mass spectrometer (GC-MS) analysis showed that the EO isolated from DES-HMAHD contained a large proportion of m -cymene and trans -linalool oxide, which were quite different from the conventionally reported L. cubeba EO. In addition, the proposed DES-HMAHD resulted in higher separation efficiency and economic value, as well as lower environmental impact, as compared with other techniques. Afterwards, the EO isolated by different methods was evaluated from the perspective of biological activity. The EO obtained by DES-HMAHD showed higher antioxidant activity (DPPH and ABTS) but lower antifungal activity, which was related to its chemical composition. In general, DES-HMAHD produced a kind of L. cubeba EO with different components, which provided a scientific foundation for the sufficient isolation of plant EO and its application in the natural products.
研究领域:
植物学
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天然产物化学
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药用植物研究
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiayi Zhang,Yumeng Zhang,Mingxuan Gu,Yingjie Wang,Ruonan Yu,Ling Weng,Chunjie Zhao,Min Zhao
Polyporus umbellatus (Pers.) Fries (PU), a medicinal fungus, contains polysaccharides (PUPs) as its primary bioactive components. In this paper, physicochemical properties, biological activities and in vitro fermentation prebiotic properties of PUPs, extracted by 90 °C hot water (PUP-W), 0.9 % NaCl (PUP-N), citric acid (PUP-S) and 0.1 M NaOH (PUP-A) were compared. The PUPs were composed of multiple monosaccharides, with significant differences in chemical composition and structure. The extracted PUPs demonstrated notable antioxidant, antitumor and prebiotic activities. With the highest yield (2.96 ± 0.12), PUP-A exhibited better biological activities due to its small molecular weight. The antioxidant ability (clearing DPPH (IC 50 = 0.64) and ABTS free radicals (IC 50 = 1.23)) of PUP-A was strong, so was the ability of ROS clearing. PUP-A significantly decreased the cell survival rate of HepG2 (IC 50 = 1090). In terms of prebiotic properties, both PUP-N and PUP-A showed substantial promoting effects on some beneficial bacteria, like Bacteroidetes at the phylum level and Lactobacillus at the genus level. In summary, our study suggests that alkali is the better solvent and provides new insights into the relationship between the structure, biological activities, and gut microbiota of PUPs, laying the foundation for better development of the role of PU.
研究领域:
微生物学
·
生物化学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-9
·
IF 4.259
Li Shan-Shan,Guan Qi-Yuan,Meng Gang,Chang Xiao-Feng,Wei Ji-Wu,Wang Peng,Kang Bin,Xu Jing-Juan,Chen Hong-Yuan
Better understanding the drug action within cells may extend our knowledge on drug action mechanisms and promote new drugs discovery. Herein, we studied the processes of drug induced chemical changes on proteins and nucleic acids in human breast adenocarcinoma (MCF-7) cells via time-resolved plasmonic-enhanced Raman spectroscopy (PERS) in combination with principal component analysis (PCA). Using three popular chemotherapy drugs (fluorouracil, cisplatin and camptothecin) as models, chemical changes during drug action process were clearly discriminated. Reaction kinetics related to protein denaturation, conformational modification, DNA damage and their associated biomolecular events were calculated. Through rate constants and reaction delay times, the different action modes of these drugs could be distinguished. These results may provide vital insights into understanding the chemical reactions associated with drug-cell interactions.
研究领域:
生物物理学
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药学
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细胞生物学
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光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）