Journal of Materials Chemistry B
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2022
·Vol. 10
·期 32
·6214-6227
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IF 7.571
Xu Zhang,Jian Li,Jin Chen,Zi-Xin Peng,Jiang-Nan Chen,Xinyi Liu,Fuqing Wu,Peng Zhang,Guo-Qiang Chen
The hierarchical three-dimensional (3D)-printing scaffolds based on microbial polyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB) were designed and used for bone tissue engineering via surface functionalization on 3D-printed (P34HB) scaffolds using polydopamine (PDA)-mediated recombinant human bone morphogenetic protein-2 (BMP2), leading to enhanced bone formation in a rat model with a calvarial critical-size bone defect. Taking advantage of the adhesive property of PDA under alkaline and aerobic conditions, osteogenic BMP2 was captured on the surface of PHA scaffolds, resulting in their enhanced osteogenic bioactivity, better stem cell adhesion and proliferation, and sustainable release of a bioactive substance over a period of 30 days. These contributed to notable differences in alkaline phosphatase (ALP) activity, mineralization, expressions of osteogenesis-related genes, as well as finally enhanced in vivo bone formation in rats. The functionalized 3D-printed P34HB scaffolds via the PDA-mediated osteogenic activity were developed as a versatile platform for bone tissue regeneration.
研究领域:
组织工程
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生物医用材料
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骨再生
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生物技术
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Bingxu Zhang,Xujian Li,Chuan Jiang,Chuanguang Wang,Haifeng Que,Cheng Zheng,Zhixiao Ji,Xudong Tao,Hongtao Xu,Changcan Shi
The effective prevention and treatment of anastomotic leakage after intestinal anastomosis for colorectal diseases is still a major clinical challenge. In order to assist intestinal anastomosis healing and avoid anastomotic leakage caused by high tension, low blood supply or infection, we designed a double-layer nanofiber intestinal anastomosis scaffold, which was composed of electrospun PTMC/PHA nanofibers as the main layer, and electrospun PVA/OHA-Gs nanofibers with antibacterial properties as the antibacterial surface layer. This double-layer scaffold has good toughness, its maximum tensile force value could reach 8 N, elongation could reach 400 %, and it has hydrophilic properties, and its contact angle was about 60°. On the basis of reducing anastomotic tension and isolating intestinal contents, this double-layer nanofiber anastomotic scaffold not only played an antibacterial effect in the short term after surgery to reduce inflammatory response, but also had the characteristics of multiple three-dimensional network structure like extracellular matrix which could promote tissue healing. The PVA/OHA-Gs@PTMC/PHA scaffold was implanted into a rabbit model simulating mechanical intestinal obstruction, and the results showed that the nanofibers of the scaffold could be degraded in vivo while maintaining a certain stability, that is, the overall structure of the PVA/OHA-Gs@PTMC/PHA scaffold would not shrink and deform due to degradation in a certain period of time. Therefore, the treatment with this scaffold showed better healing at the anastomotic site. Compared to the direct anastomosis group and pure PTMC scaffold group, the double-layer scaffold group promoted a faster return to normal anastomotic strength within 7 days. This PVA/OHA-Gs@PTMC/PHA double-layer nanofiber flexible scaffold appears to be a promising therapeutic strategy to prevent anastomotic leakage after intestinal anastomosis.
研究领域:
生物医学工程
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组织工程
·
外科手术研究
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产品关联
DOI
PubMed
高影响因子
Materials Research Express
·
2019
·Vol. 6
·期 7
·075328
·
IF 1.449
Kun Li,Wen-Wen Zhang,Xiao-Juan Shi,Lan-Xiang Liu,Kai Li,Juan Xu,Jin-Ju Ma,Hong Zhang
Microbial production of polyhydroxyalkanoic acid (PHA) with customized features has been attempted by humans through metabolic engineering. However, few studies have explored the green chemical synthesis of customized PHA using hydroxy fatty acids. In the present study, new mcl-PHA having C16-co-C16, blocked by esterification of hydroxy groups in the side chain, was synthesized by melt polycondensation using aleuritic acid (9, 10, 16-trihydroxyhexadecanoic acid) and lactic acid as long-chain fatty acid monomers and active blocking molecule, respectively. The results showed that when the two monomers reacted at 150 °C for 24 h, the properties of PHA were largely retained, along with free hydroxyl groups as much as possible. Comparison of the effect of different ratios of active small molecule (lactic acid) on the properties of new mcl-PHA revealed that copolymerization mainly happened between the long-chain monomers, and lactic acid served as a blocker by esterification in the side chain on the hydroxy groups of mcl-PHA. Further, the properties of side chain-esterified mcl-PHA (SCE-mcl-PHA) changed from rigid to soft with increasing ratio of active lactic acid monomer. The tensile strength of mcl-PHA was similar to that of commercially available products PLA and PHBV. The mechanical properties of SCE-mcl-PHA significantly changed by adding only 5 wt% lactic acid monomer. Besides, the cell proliferation analysis showed that the new PHA had minimum cytotoxicity, which was close to PLA and PHBV. Therefore, these new mcl-PHA and SCE-mcl-PHA have great potential for application in food, medicine, cosmetics, and other fields, and hence require further investigation.
研究领域:
生物技术
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材料科学
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化学工程
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聚合物化学
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产品关联
DOI
高影响因子
JOURNAL OF MICROENCAPSULATION
·
2024
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IF 3
Lili Li,Lin Jing,Zijun Tang,Jingguo Du,Yonglong Zhong,Xu Liu,Mingqing Yuan
Aim To construct a novel nano-carrier with dual ligands to achieve superior anti-tumour efficacy and lower toxic side effects.Methods Liposomes were prepared by thin film hydration method. Ultraviolet, high performance liquid chromatography, nano-size analyser, ultrafiltration centrifugation, dialysis, transmission electron microscope, flow cytometry, Cell Counting Kit-8, confocal laser scanning microscopy, transwell, and tumorsphere assay were used to study the characterisations, cytotoxicity, and in vitro targeting of dg-Bcan targeting peptide (BTP-7)/pHA-temozolomide (TMZ)/tetra(4-carboxyphenyl)porphyrin (TCPP)-Lip.Results BTP-7/pHA-TMZ/TCPP-Lip was a spheroid with a mean diameters of 143 ± 3.214 nm, a polydispersity index of 0.203 ± 0.025 and a surface charge of −22.8 ± 0.425 mV. The drug loadings (TMZ and TCPP) are 7.40 ± 0.23% and 2.05 ± 0.03% (mg/mg); and the encapsulation efficiencies are 81.43 ± 0.51% and 84.28 ± 1.64% (mg/mg). The results showed that BTP-7/pHA-TMZ/TCPP-Lip presented enhanced targeting and cytotoxicity.Conclusion BTP-7/pHA-TMZ/TCPP-Lip can specifically target the tumour cells to achieve efficient drug delivery, and improve the anti-tumour efficacy and reduces the systemic toxicity.
研究领域:
药学
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肿瘤学
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2019
·
IF 1.398
Yufeng Sun,Jiameng Liu,Yatao Huang,Minmin Li,Jia Lu,Nuo Jin,Yan He,Bei Fan
Fresh kidney beans (Phaseolus vulgaris L.) are common green vegetable that are widely cultured and consumed worldwide. The benefits of fresh kidney beans are highly substantial, but at the same time, toxicity caused by phytohemagglutinin (PHA) has raised concerns. PHA from fresh kidney beans accounted for 40% of poisoning events induced by poisonous plants in China from 2004 to 2013. However, an understanding of PHA levels in fresh kidney beans remains elusive. An analysis method for PHA based on agglutination of PHA to erythrocyte cell was established. We investigated the effect of cultivar, bean part, maturity, and cooking method on PHA content in fresh kidney beans. We demonstrated significant differences in the distribution of PHA among various predominant, fresh Chinese kidney bean cultivars and parts. One major Chinese strain, Zihuayoudou, was found to contain the highest amount of PHA, which was abundant in fresh kidney bean seeds. Moreover, PHA concentrations were found to decrease with the maturity of the fresh product. Further, cooking methods had a significant effect on PHA levels in fresh kidney beans. PHA could be effectively removed when stir-frying for more than 18 min, or braising for more than 10 min. These findings provide insight into the PHA content of fresh kidney beans in China and will be helpful to guide food safety measures.
研究领域:
食品科学
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毒理学
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植物生物学
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DOI
高影响因子
Bioresource Technology Reports
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2024
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IF 0
Yi Zhang,Yuqi Zou,Qiuyue Tao,Mingfeng Yang,Marie Harder,Han Wang,Bo Liang,Ioanna Ntaikou,Georgia Antonopoulou,Gerasimos Lyberatos
Polyhydroxyalkanoate (PHA) is a kind of cellular biopolymers, and a renewable alternative to conventional plastics. To promote low-cost PHA production, this study developed a simple and scalable process to recover this polymer from a phenol-fed mixed culture. Cultures containing >50 % PHA were first acidified then treated with NaClO, leading to digestion of cellular materials by HClO. NaClO dosage, HCl/NaClO ratio, and treatment time were tested for their effects on product Purity and Recovery rate. Compared with NaClO alone which obtained 55–74 % Purity and 57–73 % Recovery, acidification improved the extraction efficiency to 62–83 % Purity and 75–97 % Recovery of cellular PHA. Statistical analysis was performed on dosage, ratio and treatment time. The chemical cost of obtaining 1 kg pure PHA ranged $7–10, but can be greatly reduced by applying a denser culture. However, HClO reduced the molecular weight of the PHA products, which needs to be addressed in future studies.
研究领域:
生物高分子材料
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微生物发酵
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生物降解塑料
✓ 已验证
DOI
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Mingfeng Yang,Yuqi Zou,Xiao Wang,Xiang Liu,Chunli Wan,Marie Harder,Qun Yan,Jiangkun Nan,Ioanna Ntaikou,Georgia Antonopoulou,Gerasimos Lyberatos,Yi Zhang
Polyhydroxyalkanoates (PHA) are a class of intracellular storage polymers with the potential to supplement/replace petroleum-based plastics. Production of PHA from waste organics can reduce its cost, but toxic carbon sources such as simple aromatics are seldom used. This study investigates the feasibility of mixed toxic phenolics as substrates, utilizing a phenol-acclimated consortium of high PHA accumulation capability. Phenol was employed as the main feedstock, and four kinds of mono- and di-chlorinated phenols (CPs) were individually mixed with it as possible impurities. The inclusion of CPs was found to reduce substrate removal and PHA accumulation rates, compared to phenol alone. The degree of inhibition was positively-correlated with CP concentration, but the mechanisms differed. 2- and 3-chlorinated phenols formed dark, toxic, dead-end products; 4-chlorinated phenol (4-CP) appeared to exert competitive inhibition; and 2,4-dichlorinated phenol acted like an inert component. Transformation pathway study discovered that phenol was degraded via meta-cleavage, while 2- and 3-CPs were transformed into 3-chlorocatechol as the intermediate. FTIR, DSC , NMR, HPLC and elemental analysis were carried out to characterize the extracted polymer. The results show that phenol was converted to poly(3-hydroxybutyrate), while the inclusion of mono-chlorinated phenol promoted the production of PHA containing 3-hydroxyvalerate (3HV) monomers . A recovery test after mild CP exposure found that the affected biomass was able to resume phenol degradation and PHA synthesis immediately after simple aeration, indicating its robustness and applicability in a real industrial setting.
研究领域:
环境生物学
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微生物学
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生物化学
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2019
·
IF 5.667
Lin-Ping Yu,Xu Zhang,Dai-Xu Wei,Qiong Wu,Xiao-Ran Jiang,Guo-Qiang Chen
Fluorescent materials play an important role in biomedical fields. However, the main types of fluorescent materials suffer from several disadvantages especially the biotoxicity, which largely restrict its wider applications in biological fields. In this study, a highly efficient rare-earth-modified fluorescent material was successfully designed and fabricated based on polyhydroxyalkanoates, which are known as biodegradable and biocompatible materials. A new Functional-PHA polymer was microbially synthesized by engineered Halomonas bluephagenesis and was used as a basal matrix to generate the rare-earth-modified PHA. N-Acetyl-l-cysteine-grafted PHA (NAL-grafted-PHA) was first produced via a UV-initiated thiol–ene click reaction and the rare earth metal ions (Eu3+ and Tb3+) were subsequently chelated onto the NAL-grafted-PHA through the coordination effect. The composite material exhibited intense photoluminescence properties under UV laser excitation, indicating the excellent features as fluorescent material. The enhanced hydrophilicity and superior biocompatibility of rare-earth-chelated PHA were confirmed, suggesting its great potential application value in biomedical fields.
研究领域:
材料科学
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生物技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2019
·
IF 4.213
Haiyu Ni,Shuwen Xu,Pengfei Gu,Adelijiang wusiman,Yue Zhang,Tianxin Qiu,Zhenguang Liu,Han Ni,Yuanliang Hu,Jiaguo Liu,Yi Wu,Deyun Wang
This study aimed to optimize the preparation conditions for cetyltrimethylammonium bromide-modified Polygonatum sibiricum polysaccharide cubosomes (CTAB-modified PSP-Cubs) by response surface methodology (RSM). Glyceryl-monooleate (GMO) was used as the lipid base for the cubosomes. The optimal preparation conditions of CTAB-modified PSP-Cubs were as follows: the mass percentage of PSP to GMO (X 1 ), Poloxamer 407 (F127) to GMO (X 2 ) and water to GMO (X 3 ) was 1.4%, 9% and 50%, respectively. The encapsulation efficiency (EE) of CTAB-modified PSP-Cubs under the experimental conditions was 62.4 ± 3.6%, which was close to our predicted value. The particle size, polydispersity index (PDI) and zeta potential of CTAB-modified PSP-Cubs were 427.7 ± 8.0 nm , 0.236 ± 0.024 and 19.2 ± 0.4 mV, respectively. Specific modifications were able to slow down in vitro release behaviors and reduce cytotoxicity to some extent. The effects of CTAB-modified PSP-Cubs on splenic lymphocytes were also investigated. When splenic lymphocytes were treated with CTAB-modified PSP-Cubs together with LPS or PHA , the results showed more favorable effects on cellular proliferation than blank cubosomes or free PSP at certain concentrations.
研究领域:
药剂学
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免疫学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2022
·
IF 8.91
Yuqi Zou,Mingfeng Yang,Qiuyue Tao,Keliang Zhu,Xiang Liu,Chunli Wan,Marie K. Harder,Qun Yan,Bo Liang,Ioanna Ntaikou,Georgia Antonopoulou,Gerasimos Lyberatos,Yi Zhang
PHAs are a form of cellular storage polymers with diverse structural and material properties, and their biodegradable and renewable nature makes them a potential green alternative to fossil fuel-based plastics. PHAs are obtained through extraction via various mechanical, physical and chemical processes after their intracellular synthesis. Most studies have until now focused on pure cultures, while information on mixed microbial cultures (MMC) remains limited. In this study, ultrasonic (US) disruption and alkaline digestion by NaOH were applied individually and in combination to obtain PHAs products from an acclimated MMC using phenol as the carbon source. Various parameters were tested, including ultrasonic sound energy density , NaOH concentration, treatment time and temperature, and biomass density. US alone caused limited cell lysis and resulted in high energy consumption and low efficiency. NaOH of 0.05–0.2 M was more efficient in cell disruption, but led to PHAs degradation under elevated temperature and prolonged treatment. Combining US and NaOH significantly improved the overall process efficiency, which could reduce energy consumption by 2/3rds with only minimal PHAs degradation. The most significant factor was identified to be NaOH dosage and treatment time, with US sound energy density playing a minor role. Under the semi-optimized condition (0.2 M NaOH, 1300 W L -1 , 10 min), over 70% recovery and 80% purity were achieved from a 3 g L −1 MMC slurry of approximately 50% PHAs fraction. The material and thermal properties of the products were analyzed, and the polymers obtained from US + NaOH treatments showed comparable or higher molecular weight to previously reported results. The products also exhibited good thermal stability and rheological properties, compared to the commercial standard. In conclusion, the combined US and NaOH method has the potential in real application as an efficient process to obtain high quality PHAs from MMC, and cost-effectiveness can be further optimized.
研究领域:
生物技术
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环境工程
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2024
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IF 10.7
Yuan Ding,Ruohan Chen,Jianfen Zhou,Yanning Bao,Nana Meng,Xudong Zheng,Shengmin Yang,Jiasheng Lu,Zhixuan Jiang,Yu Liu,Cao Xie,Linwei Lu,Weiyue Lu
There remain several intractable challenges for chemotherapy in glioma treatment, including the blood-brain barrier (BBB), blood-brain tumor barrier (BBTB), and tumor heterogeneity caused by cancer stem cells (CSCs), which are resistant to conventional chemotherapy. Here, we established a nano strategy to kill glioma cells and CSCs, combining carfilzomib and bis(diethyldithiocarbamate)copper. The synergistic drug combination disturbed cell protein metabolism at different stages and induced apoptosis and cuproptosis. The Y-shaped targeting ligand pHA-VAP-modified nanodiscs were designed to help the chemotherapeutic agents cross the BBB/BBTB and finally accumulate in tumor site. This all-stage targeting and all-stage treatment nanomedicine significantly prolonged the survival in glioma-bearing mice and might inspire the rational design of advanced drug delivery platforms.
研究领域:
肿瘤学
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药理学
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纳米技术
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
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2024
·
IF 10.5
Juanhui Lin,Yuansong Wei,Xiaxian Gu,Miaoru Liu,Mengru Wang,Renxiang Zhou,Duowu Zou,Lichen Yin,Chunhua Zhou,Duanmin Hu
Severe acute pancreatitis (SAP) is an inflammatory disease of the pancreas accompanied with intestinal injury, and effective therapeutic modalities are still highly lacking. Herein, a facile and effective nanotherapeutics (pHA@IBNCs) is developed to alleviate pancreatic inflammation and restore intestinal barrier for SAP treatment. Epigallocatechin gallate (EGCG, an anti-oxidant), interleukin-22 (IL-22, an anti-inflammatory and epithelial barrier-protecting cytokine), and bovine serum albumin (a framework protein), are assembled via non-covalent interactions to form nanocomplexes (IBNCs). Then, phenylboronic acid-modified hyaluronic acid (pHA) is synthesized and coated onto IBNCs via formation of the reversible boronate ester bonds to obtain pHA@IBNCs. Upon intravenous injection, pHA@IBNCs could efficiently accumulate at the lesion sites of sodium taurocholate (STC)-induced SAP mice, based on their prolonged blood circulation time and pHA-mediated targeting of activated intestinal epithelial cells and macrophages. Inside the inflammatory microenvironment, over-produced reactive oxygen species (ROS) trigger the shedding of the pHA layer and release of the drug payloads. Thereby, EGCG cooperates with IL-22 to attenuate pancreatitis and restore the intestinal barrier by scavenging ROS, suppressing pro-inflammatory cytokines secretion, and promoting the repair of intestinal epithelia. Such a nano-therapeutic approach targeting multiple pathological events may serve as a promising paradigm for the effective management of SAP.
研究领域:
纳米医学
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炎症学
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胃肠病学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）